Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,825,783 C→T 62.7% intergenic (‑135/‑52) norR ← / → norV anaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,825,7830CT62.7% 124.6 / 168.0 142intergenic (‑135/‑52)norR/norVanaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin
Reads supporting (aligned to +/- strand):  ref base C (33/20);  new base T (43/46);  total (76/66)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

Only 100 of 143 total aligned reads displayed.

AACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCG  >  CP009273/2825634‑2825928
                                                                                                                                                     |                                                                                                                                                 
aaCGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATc                                                                                                                                                   >  2:1650517/1‑150 (MQ=255)
         aCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAg                                                                                                                                          <  1:135233/150‑1 (MQ=255)
         aCTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAg                                                                                                                                          <  1:2562225/150‑1 (MQ=255)
         aCTCATCTGTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAg                                                                                                                                          <  2:2220646/150‑1 (MQ=255)
              tCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGa                                                                                                                                     >  2:2022871/1‑150 (MQ=255)
               cTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAc                                                                                                                                    >  1:2715867/1‑150 (MQ=255)
               cTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGAc                                                                                                                                    >  1:2027387/1‑150 (MQ=255)
                   gCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCt                                                                                                                                <  2:4659033/150‑1 (MQ=255)
                   gCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCt                                                                                                                                <  2:1250850/150‑1 (MQ=255)
                    ccTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCtt                                                                                                                               <  1:381440/150‑1 (MQ=255)
                    ccTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCtt                                                                                                                               <  1:381441/150‑1 (MQ=255)
                      tCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCGATTTGCAATTAGCAAGACATCtttt                                                                                                                             >  1:2058018/1‑150 (MQ=255)
                         cTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAg                                                                                                                          <  2:730687/150‑1 (MQ=255)
                                tttGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGc                                                                                                                   <  2:2349913/150‑1 (MQ=255)
                                 ttGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCt                                                                                                                  <  1:1178933/150‑1 (MQ=255)
                                 ttGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCt                                                                                                                  >  1:2551570/1‑150 (MQ=255)
                                  tGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTg                                                                                                                 <  1:184038/150‑1 (MQ=255)
                                    aCTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCACTTAGCAAGACATCTTTTTAGACCACGCTGaa                                                                                                               >  2:236919/1‑150 (MQ=255)
                                    aCTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGaa                                                                                                               <  1:4441578/150‑1 (MQ=255)
                                      tataGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATa                                                                                                             >  2:33736/1‑150 (MQ=255)
                                      tataGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATa                                                                                                             >  2:2462699/1‑150 (MQ=255)
                                       ataGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATaa                                                                                                            <  1:2032022/150‑1 (MQ=255)
                                        taGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATaaa                                                                                                           <  1:2238823/150‑1 (MQ=255)
                                          gATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAAtt                                                                                                         >  1:4158568/1‑150 (MQ=255)
                                            tatTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGa                                                                                                       <  2:4222532/150‑1 (MQ=255)
                                              ttGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAgg                                                                                                     >  2:3882680/1‑150 (MQ=255)
                                              ttGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAgg                                                                                                     >  2:4652562/1‑150 (MQ=255)
                                                   atatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCt                                                                                                <  1:4737394/150‑1 (MQ=255)
                                                    tatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGTAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTa                                                                                               >  2:2732613/1‑150 (MQ=255)
                                                    tatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTa                                                                                               <  1:3307939/150‑1 (MQ=255)
                                                    tatCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTa                                                                                               <  1:3307949/150‑1 (MQ=255)
                                                     atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                              <  2:1255720/150‑1 (MQ=255)
                                                     atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                              >  2:1398546/1‑150 (MQ=255)
                                                     atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                              >  2:4796739/1‑150 (MQ=255)
                                                     atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                              >  2:4316910/1‑150 (MQ=255)
                                                     atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                              >  2:4316904/1‑150 (MQ=255)
                                                     atCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAAATATATTTATAGAGTAAAAACAATCAGATAAAAAACTTGCACGCAATCTGCAATTAGAAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAt                                                                                              >  2:1398650/1‑150 (MQ=255)
                                                        gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTc                                                                                           <  1:4770930/150‑1 (MQ=255)
                                                        gACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTc                                                                                           <  1:2777510/150‑1 (MQ=255)
                                                                   tgatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGa                                                                                >  2:402993/1‑150 (MQ=255)
                                                                    gatAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTGAGAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGaa                                                                               <  1:2609970/150‑1 (MQ=255)
                                                                     atAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGaaa                                                                              <  2:3287042/150‑1 (MQ=255)
                                                                      tAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGaaaa                                                                             >  2:3845763/1‑150 (MQ=255)
                                                                         tCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATa                                                                          <  2:2764390/150‑1 (MQ=255)
                                                                         tCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATa                                                                          <  2:334489/150‑1 (MQ=255)
                                                                          cATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATaa                                                                         <  1:8847/150‑1 (MQ=255)
                                                                           aTTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAc                                                                        >  2:1902935/1‑150 (MQ=255)
                                                                              ttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcatt                                                                     >  2:3780112/1‑150 (MQ=255)
                                                                              ttGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcatt                                                                     >  2:2082881/1‑150 (MQ=255)
                                                                                gACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattca                                                                   <  1:402993/150‑1 (MQ=255)
                                                                                 actactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattcat                                                                  <  2:230477/150‑1 (MQ=255)
                                                                                  atactCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAAcattcatt                                                                 <  2:3640424/149‑1 (MQ=255)
                                                                                       cATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGtt                                                            <  1:1011985/150‑1 (MQ=255)
                                                                                          tAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGt                                                         >  1:4008151/1‑150 (MQ=255)
                                                                                          tAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGt                                                         >  1:1797804/1‑150 (MQ=255)
                                                                                           aaTGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTc                                                        >  2:283014/1‑150 (MQ=255)
                                                                                           aaTGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTc                                                        >  2:1701253/1‑150 (MQ=255)
                                                                                             tGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCaa                                                      >  2:1794543/1‑150 (MQ=255)
                                                                                              gggCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAAc                                                     >  2:151061/1‑150 (MQ=255)
                                                                                              gggCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAAc                                                     <  2:1076409/150‑1 (MQ=255)
                                                                                               ggCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACg                                                    >  1:1749124/1‑150 (MQ=255)
                                                                                                      ttttATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTgg                                             >  1:4393193/1‑150 (MQ=255)
                                                                                                       tttatttaTAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTggg                                            >  1:1736099/1‑150 (MQ=255)
                                                                                                                 gagTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGa                                  <  1:4054439/150‑1 (MQ=255)
                                                                                                                 gagTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGa                                  <  2:4043017/150‑1 (MQ=255)
                                                                                                                  agTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCACTTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAt                                 <  2:847550/150‑1 (MQ=255)
                                                                                                                  agTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGAt                                 <  2:3861952/150‑1 (MQ=255)
                                                                                                                        aaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcac                           >  1:2483165/1‑150 (MQ=255)
                                                                                                                        aaCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcac                           <  1:1001458/150‑1 (MQ=255)
                                                                                                                         aCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacg                          >  1:1898498/1‑150 (MQ=255)
                                                                                                                         aCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacg                          >  1:3845735/1‑150 (MQ=255)
                                                                                                                         aCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacg                          >  2:1330623/1‑150 (MQ=255)
                                                                                                                         aCAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacg                          >  1:1899792/1‑150 (MQ=255)
                                                                                                                            aTCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggca                       >  2:256603/1‑150 (MQ=255)
                                                                                                                            aTCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggca                       <  1:2797392/150‑1 (MQ=255)
                                                                                                                            aTCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggca                       >  2:3846416/1‑150 (MQ=255)
                                                                                                                            aTCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggca                       >  2:480837/1‑150 (MQ=255)
                                                                                                                               aGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGACGTGCGTGATTTTcacggcacgg                    >  1:536011/1‑150 (MQ=255)
                                                                                                                               aGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggcacgg                    >  1:2972350/1‑150 (MQ=255)
                                                                                                                               aGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggcacgg                    >  1:535997/1‑150 (MQ=255)
                                                                                                                               aGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTcacggcacgg                    >  1:535924/1‑150 (MQ=255)
                                                                                                                                gATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGa                   <  1:694864/150‑1 (MQ=255)
                                                                                                                                gATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGa                   <  1:694086/150‑1 (MQ=255)
                                                                                                                                 aTAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGaa                  <  2:1785356/150‑1 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCTCGGCACGGact                 >  2:2413748/1‑148 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                 >  2:2414442/1‑150 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                 <  1:33736/150‑1 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                 >  2:787131/1‑150 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                 >  2:44685/1‑150 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                 >  2:44339/1‑150 (MQ=255)
                                                                                                                                  tAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAat                 <  1:2462699/150‑1 (MQ=255)
                                                                                                                                    aaaaaCTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATGGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAatat               <  1:1700401/150‑1 (MQ=255)
                                                                                                                                     aaaaCTGGCACGCAATTTGAAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAtata              <  2:3876479/150‑1 (MQ=255)
                                                                                                                                     aaaaCTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAtata              <  1:1121250/150‑1 (MQ=255)
                                                                                                                                       aaCTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATaaa            >  2:849117/1‑150 (MQ=255)
                                                                                                                                          tGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACg         >  2:646043/1‑150 (MQ=255)
                                                                                                                                               cGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTgcg    >  1:2005098/1‑150 (MQ=255)
                                                                                                                                                gCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTgcgc   >  2:3220766/1‑150 (MQ=255)
                                                                                                                                                 cAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGcgcg  <  1:380474/150‑1 (MQ=255)
                                                                                                                                                 cAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGcgcg  <  1:380471/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
AACGGAAAAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCG  >  CP009273/2825634‑2825928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  CP009273/2825641‑2825930
                                                                                                                                              |                                                                                                                                                   
AAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATT                                                                                                                                              <  SRR19973081.4339549/150‑1 (MQ=60)
  ACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAG                                                                                                                                            <  SRR19973081.2578178/150‑1 (MQ=60)
  ACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAG                                                                                                                                            <  SRR19973081.1898497/150‑1 (MQ=60)
  ACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAG                                                                                                                                            <  SRR19973081.136515/150‑1 (MQ=60)
  ACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAG                                                                                                                                            <  SRR19973081.2797920/150‑1 (MQ=60)
        CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGAC                                                                                                                                      >  SRR19973081.2732647/1‑150 (MQ=60)
        CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGAC                                                                                                                                      >  SRR19973081.2040090/1‑150 (MQ=60)
        CTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGAC                                                                                                                                      >  SRR19973081.2040071/1‑150 (MQ=60)
         TTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCGGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACA                                                                                                                                     <  SRR19973081.4689486/150‑1 (MQ=60)
             CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTT                                                                                                                                 <  SRR19973081.383923/150‑1 (MQ=60)
             CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTT                                                                                                                                 <  SRR19973081.383940/150‑1 (MQ=60)
             CCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTT                                                                                                                                 <  SRR19973081.383941/150‑1 (MQ=60)
               TCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCGATTTGCAATTAGCAAGACATCTTTT                                                                                                                               >  SRR19973081.2071002/1‑150 (MQ=60)
                        ATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACG                                                                                                                      >  SRR19973081.1574186/1‑150 (MQ=60)
                          TTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCT                                                                                                                    <  SRR19973081.1186460/150‑1 (MQ=60)
                          TTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCACGACATCTTTTTAGAACACGCT                                                                                                                    >  SRR19973081.2567397/1‑150 (MQ=60)
                          TTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCT                                                                                                                    >  SRR19973081.2567467/1‑150 (MQ=60)
                           TGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTG                                                                                                                   <  SRR19973081.4036537/150‑1 (MQ=60)
                           TGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTG                                                                                                                   <  SRR19973081.185674/150‑1 (MQ=60)
                            GACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGA                                                                                                                  >  SRR19973081.1181796/1‑150 (MQ=60)
                             ACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAA                                                                                                                 <  SRR19973081.4468276/150‑1 (MQ=60)
                             ACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAA                                                                                                                 >  SRR19973081.4811242/1‑150 (MQ=60)
                                ATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAA                                                                                                              <  SRR19973081.2044752/150‑1 (MQ=60)
                                 TAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAA                                                                                                             <  SRR19973081.2253000/150‑1 (MQ=60)
                                   GATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATT                                                                                                           >  SRR19973081.4183977/1‑150 (MQ=60)
                                      ATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAG                                                                                                        >  SRR19973081.772676/1‑150 (MQ=60)
                                            ATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCT                                                                                                  <  SRR19973081.4766053/150‑1 (MQ=60)
                                             TATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTA                                                                                                 <  SRR19973081.3328163/150‑1 (MQ=60)
                                             TATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTA                                                                                                 <  SRR19973081.3328153/150‑1 (MQ=60)
                                              ATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTAT                                                                                                >  SRR19973081.657469/1‑150 (MQ=60)
                                                 GACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTC                                                                                             <  SRR19973081.2794514/150‑1 (MQ=60)
                                                 GACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTC                                                                                             <  SRR19973081.4799760/150‑1 (MQ=60)
                                                    CATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTAT                                                                                          >  SRR19973081.2457383/1‑150 (MQ=60)
                                                             GATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTGAGAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAA                                                                                 <  SRR19973081.2626236/150‑1 (MQ=60)
                                                                   CATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAA                                                                           <  SRR19973081.8976/150‑1 (MQ=60)
                                                                   CATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAA                                                                           >  SRR19973081.1965941/1‑150 (MQ=60)
                                                                   CATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAA                                                                           >  SRR19973081.3855052/1‑150 (MQ=60)
                                                                         GACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCA                                                                     <  SRR19973081.405564/150‑1 (MQ=60)
                                                                                CATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTT                                                              <  SRR19973081.1018838/150‑1 (MQ=60)
                                                                                   TAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGT                                                           >  SRR19973081.4032491/1‑150 (MQ=60)
                                                                                   TAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGT                                                           >  SRR19973081.1809550/1‑150 (MQ=60)
                                                                                      TGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAA                                                        >  SRR19973081.3334167/1‑150 (MQ=60)
                                                                                        GGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACG                                                      >  SRR19973081.1760592/1‑150 (MQ=60)
                                                                                             AATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTATCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACT                                                 <  SRR19973081.2601018/150‑1 (MQ=60)
                                                                                               TTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGG                                               >  SRR19973081.4419705/1‑150 (MQ=60)
                                                                                                TTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGG                                              >  SRR19973081.1747489/1‑150 (MQ=60)
                                                                                                TTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGG                                              >  SRR19973081.1747525/1‑150 (MQ=60)
                                                                                                   ATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAG                                           >  SRR19973081.3254538/1‑150 (MQ=60)
                                                                                                          GAGTAAAAACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGA                                    <  SRR19973081.4078911/150‑1 (MQ=60)
                                                                                                                 AACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCAC                             <  SRR19973081.1008252/150‑1 (MQ=60)
                                                                                                                 AACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCAC                             >  SRR19973081.2498602/1‑150 (MQ=60)
                                                                                                                  ACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACG                            >  SRR19973081.3869365/1‑150 (MQ=60)
                                                                                                                  ACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACG                            >  SRR19973081.1911960/1‑150 (MQ=60)
                                                                                                                  ACAATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACG                            >  SRR19973081.1910659/1‑150 (MQ=60)
                                                                                                                     ATCAGATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCA                         <  SRR19973081.2814452/150‑1 (MQ=60)
                                                                                                                        AGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGACGTGCGTGATTTTCACGGCACGG                      >  SRR19973081.539830/1‑150 (MQ=60)
                                                                                                                        AGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGG                      >  SRR19973081.2990659/1‑150 (MQ=60)
                                                                                                                        AGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGG                      >  SRR19973081.539816/1‑150 (MQ=60)
                                                                                                                        AGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGG                      >  SRR19973081.539742/1‑150 (MQ=60)
                                                                                                                         GATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGA                     <  SRR19973081.698736/150‑1 (MQ=60)
                                                                                                                         GATAAAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGA                     <  SRR19973081.699517/150‑1 (MQ=60)
                                                                                                                           TAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   <  SRR19973081.2477963/150‑1 (MQ=60)
                                                                                                                           TAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAAT                   <  SRR19973081.34148/150‑1 (MQ=60)
                                                                                                                             AAAAACTGGCACGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATGGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATAT                 <  SRR19973081.1711551/150‑1 (MQ=60)
                                                                                                                              AAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATA                <  SRR19973081.1128583/150‑1 (MQ=60)
                                                                                                                                        CGCAATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCG      >  SRR19973081.2017607/1‑150 (MQ=60)
                                                                                                                                          CAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCG    <  SRR19973081.382970/150‑1 (MQ=60)
                                                                                                                                          CAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCG    <  SRR19973081.382973/150‑1 (MQ=60)
                                                                                                                                            ATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGACTATAAAACGCTGCGCGGC  >  SRR19973081.2331393/1‑150 (MQ=60)
                                                                                                                                            ATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  SRR19973081.2331694/1‑150 (MQ=60)
                                                                                                                                            ATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  SRR19973081.255584/1‑150 (MQ=60)
                                                                                                                                            ATTTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  SRR19973081.255590/1‑150 (MQ=60)
                                                                                                                                              |                                                                                                                                                   
AAACTCATCTTTGCCTCACTGTCAATTTGACTATAGATATTGTCATATCGACCATTTGATTGATAGTCATTTTGACTACTCATTAATGGGCATAATTTTATTTATAGAGTAAAAACAATCAGATAAAAAACTGGCACGCAATCTGCAATTAGCAAGACATCTTTTTAGAACACGCTGAATAAATTGAGGTTGCTATGTCTATTGTGGTGAAAAATAACATTCATTGGGTTGGTCAACGTGACTGGGAAGTGCGTGATTTTCACGGCACGGAATATAAAACGCTGCGCGGC  >  CP009273/2825641‑2825930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑