Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A7 F1 I2 R1 | 766 | 64.2 | 3232166 | 90.9% | 2938038 | 104.5 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,709,561 | (C)6→7 | coding (915/1692 nt) | eptB ← | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,709,555 | 1 | . | C | 100.0% | 200.4 / NA | 58 | G307G (GGC→GGG) | eptB | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (30/28); total (30/28) | |||||||||||
GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCC > NC_000913/3709418‑3709687 | gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c < 2:529414/139‑1 (MQ=255)gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c < 1:226026/139‑1 (MQ=255) aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c > 2:833133/1‑136 (MQ=255) aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c < 1:833133/136‑1 (MQ=255) gtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctg < 2:1269267/139‑4 (MQ=255) tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 2:1054758/1‑135 (MQ=255) ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTa < 1:585374/139‑1 (MQ=255) tGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑c < 1:1504646/139‑1 (MQ=255) tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGc > 2:1493836/1‑139 (MQ=255) aGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCa < 1:658090/139‑1 (MQ=255) gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAg > 1:952037/1‑139 (MQ=255) aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGt < 2:952037/139‑1 (MQ=255) caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGagag > 2:1371893/1‑139 (MQ=255) gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCgg < 1:1478497/139‑1 (MQ=255) gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCgg > 1:1577070/1‑139 (MQ=255) aGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATc < 2:1270843/139‑1 (MQ=255) agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 2:296668/87‑1 (MQ=255) agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 1:296668/1‑87 (MQ=255) tCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa < 1:1614856/139‑1 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc > 2:412067/1‑139 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc > 2:1321376/1‑139 (MQ=255) aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc > 1:375189/1‑139 (MQ=255) tGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa > 2:1586664/1‑139 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa > 2:51212/1‑86 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa < 1:51212/86‑1 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt < 2:309155/128‑1 (MQ=255) aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt > 1:309155/1‑128 (MQ=255) tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccgc > 2:608217/1‑139 (MQ=255) aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctg > 2:1319152/1‑68 (MQ=255) aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctg < 1:1319152/71‑4 (MQ=255) cTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCCAACGCAGTGAGAGTTTGGTTGc < 1:645791/107‑1 (MQ=255) cTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCCAACGCAGTGAGAGTTTGGTTGc > 2:645791/1‑107 (MQ=255) cTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAg > 1:469516/1‑139 (MQ=255) ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑ttt > 1:1333863/1‑139 (MQ=255) aCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTcc < 2:469516/139‑1 (MQ=255) gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTgg > 1:1218077/1‑139 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct < 2:208131/55‑3 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct < 2:911939/55‑3 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct > 1:911939/1‑53 (MQ=255) cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct > 1:208131/1‑53 (MQ=255) gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGttt < 2:283251/139‑1 (MQ=255) gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGttt < 2:1333863/139‑1 (MQ=255) ttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTTCTCCTGGGCCAGttt < 1:412067/139‑1 (MQ=255) tctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCg < 2:375189/139‑1 (MQ=255) tctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCg < 2:1466742/139‑1 (MQ=255) gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCg < 2:1218077/139‑1 (MQ=255) gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCg < 1:1493836/139‑1 (MQ=255) ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTg < 1:1542791/74‑1 (MQ=255) ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTg > 2:1542791/1‑74 (MQ=255) ttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt > 1:805799/1‑139 (MQ=255) aTGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACa < 1:1195462/42‑1 (MQ=255) aTGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACa > 2:1195462/1‑42 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTa < 2:208801/35‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTa > 1:208801/1‑35 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGt < 2:181553/63‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGt > 1:181553/1‑63 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCgg > 1:1445224/1‑68 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCgg < 2:1445224/68‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTtctc < 1:215631/108‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTtctc > 2:215631/1‑108 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa < 2:1610748/139‑1 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa > 1:1217012/1‑139 (MQ=255) cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa > 1:1610748/1‑139 (MQ=255) cgcTGCGGAATATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa > 2:1082655/1‑139 (MQ=255) cGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTtctc < 1:697058/103‑1 (MQ=255) cGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTtctc > 2:697058/1‑103 (MQ=255) cGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGcc > 2:355189/1‑139 (MQ=255) cGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGcc > 1:949237/1‑139 (MQ=255) aTTATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaa < 1:1586664/139‑1 (MQ=255) tATCTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa < 1:1321376/139‑1 (MQ=255) cTTCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc > 2:238769/1‑139 (MQ=255) tCCGCGCCCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAccc > 1:387711/1‑139 (MQ=255) | GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATG‑CAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGA‑TTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCC > NC_000913/3709418‑3709687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |