Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,709,561 |
(C)6→7 |
coding (915/1692 nt) |
eptB ← |
KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,709,555 | 1 | . | C | 97.5%
| 128.7
/ ‑3.3
| 41 | G307G (GGC→GGG) | eptB | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (20/19); minor base A (0/1); total (20/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01 |
GTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAA > NC_000913/3709426‑3709682
|
gtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctg < 1:157135/139‑4 (MQ=255)
tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATTCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 1:733117/1‑135 (MQ=255)
cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTAc > 1:1075514/1‑139 (MQ=255)
aTCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACa < 2:540318/139‑1 (MQ=255)
gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGACCCCCC‑TGACGTACAAACATGCAACg < 1:221238/139‑1 (MQ=255)
caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGagag > 2:969076/1‑139 (MQ=255)
tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc > 1:817968/1‑105 (MQ=255)
tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc < 2:817968/105‑1 (MQ=255)
cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACATACAAACATGCAACGCAGTGAGAGTTTgg < 1:86546/139‑1 (MQ=255)
gCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:285094/139‑1 (MQ=255)
tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg < 1:338984/139‑1 (MQ=255)
ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGGTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc < 1:570509/139‑1 (MQ=255)
gCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg < 1:790056/139‑1 (MQ=255)
cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 2:171471/1‑139 (MQ=255)
cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 1:20360/1‑139 (MQ=255)
agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAgg > 1:113886/1‑139 (MQ=255)
agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg > 1:78951/1‑139 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 2:682814/128‑1 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca > 1:682814/1‑128 (MQ=255)
tGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGa > 1:463604/1‑139 (MQ=255)
aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc > 2:805563/1‑139 (MQ=255)
tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:526167/106‑1 (MQ=255)
tAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 2:526167/1‑106 (MQ=255)
aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa < 1:554959/139‑1 (MQ=255)
gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 2:87122/71‑1 (MQ=255)
gCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 1:87122/1‑71 (MQ=255)
tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:1003556/62‑1 (MQ=255)
tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 2:1003556/1‑62 (MQ=255)
aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtctc > 2:184387/1‑139 (MQ=255)
gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 1:149172/60‑1 (MQ=255)
gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 2:149172/1‑60 (MQ=255)
cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGTGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg > 1:172155/1‑139 (MQ=255)
cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg > 1:1054990/1‑139 (MQ=255)
cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGCTTGGTTGCGGTATCACAGGAGTACCCACGGAACGCCGCCAGATTTTTCTCCTggg > 2:245027/1‑139 (MQ=255)
gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt < 2:463604/139‑1 (MQ=255)
tctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg < 1:805563/139‑1 (MQ=255)
ctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCgg < 2:588570/139‑1 (MQ=255)
gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg < 1:278531/139‑1 (MQ=255)
ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGTTTGCGTTATca > 2:961932/1‑86 (MQ=255)
ttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca < 1:961932/86‑1 (MQ=255)
tctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg < 2:113886/139‑1 (MQ=255)
tctTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCATGACGTACAAACATGCAACGCAGTGAGAGTTTGATTGCGGTATCACAGGAGTAACCACGTATCGCCGCCAGAGTTTTCTCCTGGGCCAGTTTCCGCGTg < 2:733117/139‑1 (MQ=255)
ttAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc < 2:493320/36‑2 (MQ=255)
ttAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc > 1:493320/1‑35 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAAc > 2:703271/1‑47 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAAc < 1:703271/47‑1 (MQ=255)
cGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGcc > 1:858179/1‑139 (MQ=255)
aTTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGTGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaa < 2:172155/139‑1 (MQ=255)
tATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa > 1:163485/1‑139 (MQ=255)
|
GTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAA > NC_000913/3709426‑3709682
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
