Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,709,561	(C)_6→7	coding (915/1692 nt)	eptB ←	KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,709,555	1	.	C	98.3%	196.1 / ‑3.6	58	coding (921/1692 nt)	eptB	KDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (31/26); total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGC  >  NC_000913/3709418‑3709676

gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                           <  2:632433/139‑1 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  2:779552/139‑1 (MQ=255)

     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTGCCGCG‑cccccc                                                                                                                      >  2:656214/1‑139 (MQ=255)

       tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct                                                                                                                    <  2:35639/139‑3 (MQ=255)

       tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct                                                                                                                    <  2:315160/139‑3 (MQ=255)

         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga                                                                                                                  >  2:804858/1‑135 (MQ=255)

         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga                                                                                                                  >  2:132812/1‑135 (MQ=255)

                     ttGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATg                                                                                                      <  1:396214/139‑1 (MQ=255)

                        cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCaa                                                                                                   <  1:804858/139‑1 (MQ=255)

                        cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCaa                                                                                                   <  1:665553/139‑1 (MQ=255)

                        cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  1:56401/120‑1 (MQ=255)

                        cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      >  2:56401/1‑120 (MQ=255)

                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCaa                                                                                                   <  1:904338/136‑1 (MQ=255)

                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCaa                                                                                                   >  2:904338/1‑136 (MQ=255)

                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACg                                                                                                 >  2:194991/1‑138 (MQ=255)

                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACg                                                                                                 <  1:194991/138‑1 (MQ=255)

                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      >  1:786014/1‑115 (MQ=255)

                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  2:786014/115‑1 (MQ=255)

                                        cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGNNCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTgg                                                                                   >  2:46152/1‑139 (MQ=255)

                                          ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa                                                                                                              <  2:382964/110‑1 (MQ=255)

                                          ctctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa                                                                                                              >  1:382964/1‑110 (MQ=255)

                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTg                                                                                >  2:60084/1‑139 (MQ=255)

                                            ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                               <  1:908820/139‑1 (MQ=255)

                                             tGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      >  1:635506/1‑99 (MQ=255)

                                             tGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  2:635506/99‑1 (MQ=255)

                                                    cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                                                       >  1:565353/1‑139 (MQ=255)

                                                    cGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                                                       >  2:904309/1‑139 (MQ=255)

                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                             >  1:976360/1‑83 (MQ=255)

                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                             <  2:976360/83‑1 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                           >  2:829696/1‑82 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                           <  1:829696/82‑1 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                  >  2:851608/1‑139 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                  >  1:462603/1‑139 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  2:44578/87‑1 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      >  1:918600/1‑87 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  2:918600/87‑1 (MQ=255)

                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      >  1:44578/1‑87 (MQ=255)

                                                          ggTTAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTACGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGCATCACAGGAGt                                                                 <  1:237074/139‑1 (MQ=255)

                                                          ggTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                 <  1:656214/139‑1 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                             >  2:608144/1‑139 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                             >  2:281074/1‑139 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                             >  1:496055/1‑139 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                             >  2:823591/1‑139 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      >  2:575626/1‑82 (MQ=255)

                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                      <  1:575626/82‑1 (MQ=255)

                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAg                                                                                              <  2:768193/99‑1 (MQ=255)

                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAg                                                                                              >  1:768193/1‑99 (MQ=255)

                                                                            aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                           <  2:722840/63‑1 (MQ=255)

                                                                            aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                           >  1:722840/1‑63 (MQ=255)

                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct                                                                                                                    <  1:757385/66‑3 (MQ=255)

                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccct                                                                                                                    >  2:757385/1‑64 (MQ=255)

                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtttt                                         >  1:690998/1‑139 (MQ=255)

                                                                                     gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAAc                                                                                                  <  1:117464/79‑1 (MQ=255)

                                                                                     gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAAc                                                                                                  >  2:117464/1‑79 (MQ=255)

                                                                                     gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct                                      <  2:462603/139‑1 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTACTGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                >  2:971572/1‑139 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGt                                                                                       >  2:209750/1‑84 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGt                                                                                       <  1:209750/84‑1 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGGGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                >  1:279825/1‑139 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                                                       <  1:593383/116‑1 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGaa                                                       >  2:593383/1‑116 (MQ=255)

                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCATGACGTACAAACATACAACGCAGTGAGAGATTGGTTGCGGTATCAAAGGAGTAACCACGGAACGCCGCAAGATTTTTCTCCTggg                                >  1:55991/1‑139 (MQ=255)

                                                                                                  gttctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                         <  1:904309/139‑1 (MQ=255)

                                                                                                    tctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg                       <  2:496055/139‑1 (MQ=255)

                                                                                                     ctgttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCgg                      >  2:899720/1‑139 (MQ=255)

                                                                                                       gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa                                                                                                              <  1:1008992/49‑1 (MQ=255)

                                                                                                       gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa                                                                                                              >  2:1008992/1‑49 (MQ=255)

                                                                                                       gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                    <  2:861821/139‑1 (MQ=255)

                                                                                                         tctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTg                  <  1:971572/139‑1 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                                                         <  2:759580/75‑1 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATc                                                                         >  1:759580/1‑75 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                    <  2:949459/128‑1 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                    >  1:949459/1‑128 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGc         >  1:1000128/1‑139 (MQ=255)

                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGc         >  2:811297/1‑139 (MQ=255)

                                                                                                                   tgCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGGGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCt        <  2:279825/139‑1 (MQ=255)

                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGc                                                                                                     <  2:341808/44‑1 (MQ=255)

                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGc                                                                                                     >  1:341808/1‑44 (MQ=255)

                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa      >  1:53596/1‑139 (MQ=255)

                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa      >  2:291339/1‑139 (MQ=255)

                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa      >  1:145410/1‑139 (MQ=255)

                                                                                                                       cTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACCTGCAACg                                                                                                 <  1:647036/46‑1 (MQ=255)

                                                                                                                       cTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACg                                                                                                 >  2:647036/1‑46 (MQ=255)

                                                                                                                         gCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGc  <  1:60084/139‑1 (MQ=255)

                                                                                                                             aTTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                                                   <  2:449527/70‑1 (MQ=255)

                                                                                                                             aTTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGa                                                                   >  1:449527/1‑70 (MQ=255)

                                                                                                                                       gcg‑cCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                               <  1:246631/95‑1 (MQ=255)

                                                                                                                                       gcg‑cCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                               >  2:246631/1‑95 (MQ=255)

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGC  >  NC_000913/3709418‑3709676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A