Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,709,561 (C)6→7 coding (915/1692 nt) eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,709,5551.C100.0% 183.6 / NA 52G307G (GGC→GGG) eptBKDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/28);  total (24/28)

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCA  >  NC_000913/3709418‑3709688
                                                                                                                                          |                                                                                                                                     
gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                      <  1:956581/139‑1 (MQ=255)
 cAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cc                                                                                                                                     <  2:164432/139‑1 (MQ=255)
      ttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc                                                                                                                                <  2:145242/139‑2 (MQ=255)
         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATTTGCGCTGCGGATTATCTTCCGCG‑ccccccctga                                                                                                                             >  1:1223266/1‑135 (MQ=255)
         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                                 >  1:798287/1‑135 (MQ=255)
         tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc                                                                                                                                 <  2:798287/135‑1 (MQ=255)
               aTCGTGTTGCTGTAGAACCACATTTCGCTCTTCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                      <  1:1380457/124‑1 (MQ=255)
               aTCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                      >  2:1380457/1‑124 (MQ=255)
                tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                        <  2:1291522/122‑1 (MQ=255)
                tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                        >  1:1291522/1‑122 (MQ=255)
                      tGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATATGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGc                                                                                                                 <  2:485044/139‑1 (MQ=255)
                        cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa                                                                                                               <  1:1510783/139‑1 (MQ=255)
                         tGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAAc                                                                                                              <  2:1223266/139‑1 (MQ=255)
                          gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACg                                                                                                             <  2:1185665/139‑1 (MQ=255)
                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGc                                                                                                            >  1:1498110/1‑139 (MQ=255)
                           tAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGc                                                                                                            >  2:467368/1‑139 (MQ=255)
                            aGAACGACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATATTCTGTTCTTTTAATGTGTGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCa                                                                                                           <  1:936586/139‑1 (MQ=255)
                                         gCTATGCATAGTGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                              <  1:977544/139‑1 (MQ=255)
                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCTACGCAGTGAGAGTTTGGTTg                                                                                            <  1:1025953/139‑1 (MQ=255)
                                            ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                           <  2:1498110/139‑1 (MQ=255)
                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa                                                                                                               <  2:1405413/101‑1 (MQ=255)
                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCaa                                                                                                               >  1:1405413/1‑101 (MQ=255)
                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                                         >  2:497910/1‑139 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACg                                                                                                             >  2:354613/1‑96 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACg                                                                                                             <  1:354613/96‑1 (MQ=255)
                                                                           aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc                                                            <  2:604782/139‑1 (MQ=255)
                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                         <  2:159286/89‑1 (MQ=255)
                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGt                                                                                                         >  1:159286/1‑89 (MQ=255)
                                                                                cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtt                                                       >  1:910924/1‑139 (MQ=255)
                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtttt                                                     <  2:910924/139‑1 (MQ=255)
                                                                                   cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttttt                                                    >  1:264613/1‑139 (MQ=255)
                                                                                   cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttttt                                                    >  2:747102/1‑139 (MQ=255)
                                                                                        ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt                                                      >  2:634223/1‑132 (MQ=255)
                                                                                        ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt                                                      <  1:634223/132‑1 (MQ=255)
                                                                                          gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCAc                                                                       >  2:1497880/1‑113 (MQ=255)
                                                                                          gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCAc                                                                       <  1:1497880/113‑1 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                            >  2:121558/1‑139 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc                                                                                                                                <  1:591962/54‑2 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc                                                                                                                                >  2:591962/1‑53 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc                                                                                                                                   <  1:746280/51‑1 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccc                                                                                                                                   >  2:746280/1‑51 (MQ=255)
                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                            <  2:807191/121‑1 (MQ=255)
                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                            >  1:807191/1‑121 (MQ=255)
                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt                          >  2:120495/1‑139 (MQ=255)
                                                                                                                aTGTGCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTAc                       >  2:1186493/1‑139 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                       >  1:997523/1‑41 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACaa                                                                                                                       <  2:997523/41‑1 (MQ=255)
                                                                                                                   tgCGCTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCt                    <  1:121558/139‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGc                                                                                                            >  2:794099/1‑49 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGc                                                                                                            <  1:794099/49‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc                                                            <  1:1101250/97‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc                                                            >  2:1101250/1‑97 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct                                                  >  2:1182333/1‑107 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct                                                  <  1:1182333/107‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                           <  2:1138434/114‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                           >  1:1138434/1‑114 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                <  1:897440/125‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                                >  2:897440/1‑125 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                  <  1:1186493/139‑1 (MQ=255)
                                                                                                                       cTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATa                >  2:182191/1‑139 (MQ=255)
                                                                                                                       cTGCGGATTATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATa                >  1:1284137/1‑139 (MQ=255)
                                                                                                                               tATCTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa        <  1:697660/139‑1 (MQ=255)
                                                                                                                                  cTTCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc     <  2:264613/139‑1 (MQ=255)
                                                                                                                                    tCCGCGCCCCCCCTGACGTACAATCATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                                                         <  2:973233/85‑1 (MQ=255)
                                                                                                                                    tCCGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGa                                                         >  1:973233/1‑85 (MQ=255)
                                                                                                                                     ccGCGCCCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCa  <  2:1284137/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCCTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCCA  >  NC_000913/3709418‑3709688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A