Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,709,561 |
(C)6→7 |
coding (915/1692 nt) |
eptB ← |
KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,709,555 | 1 | . | C | 97.5%
| 134.3
/ ‑3.3
| 40 | coding (921/1692 nt) | eptB | KDO phosphoethanolamine transferase, Ca(2+)‑inducible |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/24); total (16/24) |
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 |
GTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC > NC_000913/3709423‑3709686
|
gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:1385399/139‑1 (MQ=255)
ttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc < 1:822139/139‑2 (MQ=255)
tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 1:1221055/1‑135 (MQ=255)
tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccctga > 2:215444/1‑135 (MQ=255)
tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cc < 2:336091/131‑1 (MQ=255)
tCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cc > 1:336091/1‑131 (MQ=255)
ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa < 2:715631/139‑1 (MQ=255)
ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa < 1:1399847/139‑1 (MQ=255)
ccATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTa < 2:1295061/139‑1 (MQ=255)
tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACaa < 2:1083793/139‑1 (MQ=255)
tCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACaa < 2:1221055/139‑1 (MQ=255)
tgtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 2:619216/1‑131 (MQ=255)
tgtTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 1:619216/131‑1 (MQ=255)
aaCCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGt < 2:634407/139‑1 (MQ=255)
acaTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTgaga > 2:993964/1‑139 (MQ=255)
caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 2:324309/109‑1 (MQ=255)
caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 1:324309/1‑109 (MQ=255)
cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg < 1:670095/98‑1 (MQ=255)
cGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg > 2:670095/1‑98 (MQ=255)
ctGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc < 1:902984/139‑1 (MQ=255)
gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 2:305951/1‑121 (MQ=255)
gTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 1:305951/121‑1 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 2:200823/88‑1 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 1:200823/1‑88 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac > 1:800054/1‑129 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac < 2:800054/129‑1 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg > 1:1139031/1‑134 (MQ=255)
aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg < 2:1139031/134‑1 (MQ=255)
aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:10973/75‑1 (MQ=255)
aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 2:10973/1‑75 (MQ=255)
aTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcg < 1:993964/139‑1 (MQ=255)
aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc < 2:813816/139‑1 (MQ=255)
aCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa > 1:1175528/1‑139 (MQ=255)
cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc > 1:989206/1‑64 (MQ=255)
cTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑ccccccc < 2:989206/65‑2 (MQ=255)
ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg < 1:398313/69‑1 (MQ=255)
ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACg > 2:398313/1‑69 (MQ=255)
tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt > 1:621893/1‑138 (MQ=255)
tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAttt < 2:621893/138‑1 (MQ=255)
cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c < 1:868098/56‑1 (MQ=255)
cAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c > 2:868098/1‑56 (MQ=255)
cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt > 1:354911/1‑89 (MQ=255)
cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt < 2:354911/89‑1 (MQ=255)
aaaaTGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGcc > 1:755853/1‑139 (MQ=255)
ctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa < 2:34786/93‑1 (MQ=255)
ctTTTAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTaa > 1:34786/1‑93 (MQ=255)
ttAATGTGCGCTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt > 1:1345886/1‑139 (MQ=255)
ttAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc < 1:306876/35‑1 (MQ=255)
ttAATGTGCGCTGCGGATTATCTTCCGCG‑cccccc > 2:306876/1‑35 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg > 2:603678/1‑122 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg < 1:603678/122‑1 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTa < 1:133550/133‑1 (MQ=255)
cgcTGCGGATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTa > 2:133550/1‑133 (MQ=255)
cacTGCGGATTATCTTCCGCG‑CCCCCCTTGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa > 1:335810/3‑139 (MQ=255)
gATTATCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGa < 1:136212/139‑1 (MQ=255)
aTCTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAAt < 2:148872/139‑1 (MQ=255)
cTTCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc < 2:1345886/139‑1 (MQ=255)
ttCCGCGCCCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAcc < 1:215444/139‑1 (MQ=255)
|
GTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACC > NC_000913/3709423‑3709686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
