Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F130 I1 R1	38	76.4	2725422	95.3%	2597327	137.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,604,632	C→A	A186E (GCA→GAA)	bglJ →	bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,604,632	0	C	A	98.7%	266.7 / ‑3.5	74	A186E (GCA→GAA)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (34/39); total (35/39)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

ACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAC  >  NC_000913_3_pae_tpiA/4604495‑4604774

acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTaa                                                                                                                                    <  1:109993/150‑1 (MQ=255)

acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTaa                                                                                                                                    <  1:363122/150‑1 (MQ=255)

acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTaa                                                                                                                                    <  3:336366/150‑1 (MQ=255)

  tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAAtg                                                                                                                                  >  2:51714/1‑150 (MQ=255)

        aTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGt                                                                                                                            <  1:552429/150‑1 (MQ=255)

           aGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGa                                                                                                                         <  4:336699/150‑1 (MQ=255)

               gTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACt                                                                                                                     >  1:786048/1‑150 (MQ=255)

                  cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTggg                                                                                                                  <  1:542312/150‑1 (MQ=255)

                  cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATAGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTggg                                                                                                                  >  2:151909/1‑150 (MQ=255)

                    ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCg                                                                                                                >  3:109564/1‑150 (MQ=255)

                    ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAAATTAATGTGATGTCGAAACTGGGCg                                                                                                                >  1:251681/1‑150 (MQ=255)

                     tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGa                                                                                                                         >  1:257745/1‑140 (MQ=255)

                     tAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGa                                                                                                                         <  2:257745/140‑1 (MQ=255)

                         cAAAGCCGGGCGTTAAGCCCGACGGAGAGAGAAATACTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTAGGCGTCact                                                                                                           >  4:415188/1‑148 (MQ=255)

                         cAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGt                                                                                                           >  2:647598/1‑150 (MQ=255)

                          aaaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGtt                                                                                                          <  1:555127/150‑1 (MQ=255)

                               cGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAc                                                                                                     <  2:142143/150‑1 (MQ=255)

                               cGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAc                                                                                                     <  2:142134/150‑1 (MQ=255)

                                    gTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                                                                >  1:238374/1‑150 (MQ=255)

                                     ttAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                                                               >  2:800860/1‑150 (MQ=255)

                                     ttAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                                                               <  3:243142/150‑1 (MQ=255)

                                      tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt                                                                                              >  2:494679/1‑150 (MQ=255)

                                      tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt                                                                                              >  2:307985/1‑150 (MQ=255)

                                      tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt                                                                                              >  3:455347/1‑150 (MQ=255)

                                         gCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgt                                                                                           <  2:551903/150‑1 (MQ=255)

                                           ccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg                                                                                         <  4:35815/150‑1 (MQ=255)

                                              aCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAg                                                                                      <  1:494679/150‑1 (MQ=255)

                                                ggAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAgg                                                                                     <  2:805118/150‑1 (MQ=255)

                                                ggAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCAGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGc                                                                                    >  4:503512/1‑150 (MQ=255)

                                                   gagagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGc                                                                                 >  3:481769/1‑150 (MQ=255)

                                                        aaaTATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGata                                                                            >  1:426266/1‑150 (MQ=255)

                                                            atTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGa                                                                                                                               >  4:458342/1‑95 (MQ=255)

                                                            atTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGa                                                                                                                               <  3:458342/95‑1 (MQ=255)

                                                            atTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt                                                                        <  1:377376/150‑1 (MQ=255)

                                                            atTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt                                                                        <  3:169299/150‑1 (MQ=255)

                                                             ttGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTg                                                                       <  4:354580/150‑1 (MQ=255)

                                                             ttGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTg                                                                       >  2:756713/1‑150 (MQ=255)

                                                              tGCGCGTTATGTCGCGTGCCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAACACGCTCCGCGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGt                                                                      <  1:624383/150‑1 (MQ=255)

                                                                cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTta                                                                    <  1:51714/150‑1 (MQ=255)

                                                                cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACAATCCGTGAACAT‑AAAGTTGAGTTGATGTCGAAACTGGGCGTCAGTTCTGACGCGAGTTTGTTGGAGGCCGCAGCAATTCGGtaa                                                                    >  2:245175/1‑148 (MQ=255)

                                                                cgcTTTATGTCGAGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTta                                                                    >  4:449592/1‑150 (MQ=255)

                                                                 gcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtat                                                                   <  1:307985/150‑1 (MQ=255)

                                                                           gcgTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                       >  2:73719/1‑120 (MQ=255)

                                                                           gcgTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                       <  1:73719/120‑1 (MQ=255)

                                                                               ggCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa                                                                                                  >  1:228511/1‑105 (MQ=255)

                                                                               ggCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa                                                                                                  <  2:228511/105‑1 (MQ=255)

                                                                                gCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATGTTCTGTTATGTATGCGGCATTGCg                                                    >  2:195766/1‑150 (MQ=255)

                                                                                      cAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa                                              <  4:109564/150‑1 (MQ=255)

                                                                                       aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg                                                                                                       >  1:604503/1‑92 (MQ=255)

                                                                                       aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg                                                                                                       <  2:604503/92‑1 (MQ=255)

                                                                                       aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAg                                             >  1:109423/1‑150 (MQ=255)

                                                                                       aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAg                                             >  1:823389/1‑150 (MQ=255)

                                                                                           acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                         <  2:786048/150‑1 (MQ=255)

                                                                                           acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                         >  2:711935/1‑150 (MQ=255)

                                                                                             acaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgt                                       >  1:188316/1‑150 (MQ=255)

                                                                                                aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                    <  1:151909/150‑1 (MQ=255)

                                                                                                 aTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTg                                   <  1:711935/150‑1 (MQ=255)

                                                                                                 aTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTg                                   <  1:382208/150‑1 (MQ=255)

                                                                                                              ttAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAAt                      >  2:498761/1‑150 (MQ=255)

                                                                                                              ttAAACGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAAt                      >  4:215096/1‑150 (MQ=255)

                                                                                                                  aCGCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGc                  >  3:44893/1‑150 (MQ=255)

                                                                                                                    gCAATATCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                <  2:823389/150‑1 (MQ=255)

                                                                                                                          tCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCt                            >  1:574290/1‑132 (MQ=255)

                                                                                                                          tCAAAACGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCt                            <  2:574290/132‑1 (MQ=255)

                                                                                                                            aaaaCGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtatg                                                              <  1:486997/96‑1 (MQ=255)

                                                                                                                            aaaaCGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtatg                                                              >  2:486997/1‑96 (MQ=255)

                                                                                                                            aaaaCGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCgg        >  2:43532/1‑150 (MQ=255)

                                                                                                                              aaCGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGt      <  1:125978/150‑1 (MQ=255)

                                                                                                                               aCGATCCGTGCACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGtt     >  2:52668/1‑150 (MQ=255)

                                                                                                                               aCGATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGtt     <  2:386066/150‑1 (MQ=255)

                                                                                                                                 gATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa   <  3:449592/150‑1 (MQ=255)

                                                                                                                                 gATCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa   <  4:44893/150‑1 (MQ=255)

                                                                                                                                  aTCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc  <  2:117737/150‑1 (MQ=255)

                                                                                                                                  aTCCGTGAACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAc  <  3:73754/150‑1 (MQ=255)

ACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACAT‑AAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTAC  >  NC_000913_3_pae_tpiA/4604495‑4604774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A