Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F6 I1 R1	16	59.9	2107472	93.9%	1978916	143.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,604,632	C→A	A186E (GCA→GAA)	bglJ →	bgl operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,604,632	0	C	A	97.5%	264.4 / ‑6.4	81	A186E (GCA→GAA)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (40/39); minor base G (2/0); total (42/39)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_pae_tpiA/4604489‑4604773

cgaccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATa                                                                                                                                          <  2:616522/149‑1 (MQ=255)

   ccgacTGAACAAGCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAt                                                                                                                                       <  2:421856/149‑1 (MQ=255)

        tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                  >  2:980309/1‑149 (MQ=255)

        tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                  <  2:549013/149‑1 (MQ=255)

         gAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtg                                                                                                                                 <  2:256210/149‑1 (MQ=255)

         gAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtg                                                                                                                                 >  1:263209/1‑149 (MQ=255)

          aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGAACCGTGAAGAGAAATTTAAtgt                                                                                                                                >  1:630797/1‑149 (MQ=255)

           aCAATCAGTGGTACATTAAACAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgtg                                                                                                                               <  2:507175/149‑1 (MQ=255)

            cAATCAGTGGTACATTAACCAAGGGCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTGGCGGGGCTAGTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCTGTGAACATAAATTTAATGTGa                                                                                                                              <  2:928069/149‑1 (MQ=255)

             aaTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGTCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGACGAGCAGCTTAAACGCAATATCGAGACTGACCGTGGACATGAATTTAAtttatt                                                                                                                             >  2:607066/1‑144 (MQ=255)

             aaTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAt                                                                                                                             >  2:92918/1‑149 (MQ=255)

              aTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCGACGCAATGACACGAATTGGCGAGCAGGTTAAAAGCGATATGAAGACGATACGTGGACATAAAGTTaacatagtg                                                                                                                            >  2:569602/1‑142 (MQ=255)

                   tGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGaa                                                                                                                       >  1:827918/1‑149 (MQ=255)

                    ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGTAGAGATTAATCTTGCGATTGAGGGCGCGGGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGaaa                                                                                                                      <  1:140032/149‑1 (MQ=255)

                     gTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTaa                                                                                                                                   <  2:4119/135‑1 (MQ=255)

                     gTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTaa                                                                                                                                   >  1:4119/1‑135 (MQ=255)

                      tACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGAGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCTGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATCAATTTAATGTGATGTCGAAACt                                                                                                                    >  2:253172/1‑149 (MQ=255)

                      tACAATAACAAAAGCCGCATGTAAACCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACt                                                                                                                    <  2:50907/149‑1 (MQ=255)

                          ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGc                                                                                                                <  1:571944/149‑1 (MQ=255)

                                  aGCCGGACGTGAAGGCCGACGGATAGAAAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTc                                                                                                        <  2:263209/149‑1 (MQ=255)

                                   gccttaCGTTAAGCCCGACGGGGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCATAAACGCAATATCAAAACGATCCGTTAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCt                                                                                                       <  2:270682/144‑1 (MQ=255)

                                   gCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATGAAAACGATCCGTGAACATAAATTTAATCTGATGTGGAAACTGGGCGTCAGttgt                                                                                                       >  1:547354/1‑147 (MQ=255)

                                     cGGGCGATAAGCCCAAGGAACAGAGAAATGTGGCCCGTTATGTCGCGTTGATAATGAGGGACACAAAGTGCCGAGCAACTTAAAGGCAATATCCAAACGCTCCGTGAACATAAATTCACTGTGATGTCGAAACTGGGCGTCAGTTCTGa                                                                                                     <  2:160143/149‑1 (MQ=255)

                                     cGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa                                                                                                     >  2:559728/1‑149 (MQ=255)

                                          gTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg                                                                                                >  1:15636/1‑149 (MQ=255)

                                           ttAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAgg                                                                                               <  2:827918/149‑1 (MQ=255)

                                           ttAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACACAgg                                                                                               <  2:801720/149‑1 (MQ=255)

                                            tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                                                              <  1:980309/149‑1 (MQ=255)

                                            tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAggg                                                                                              <  1:92918/149‑1 (MQ=255)

                                                   ggcGGAGAGAGAAATCGTGCGTGTTGTGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCACGTTAAACGCAATATCAAAACGATCCGTGAACATAAGTTTAATGTGATGTCGAAACAGGGCGTCAGTTCTGACGCAGGGTTGTTgg                                                                                       <  2:954957/147‑1 (MQ=255)

                                                    aCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTgg                                                                                                                  >  2:478086/1‑121 (MQ=255)

                                                    aCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTgg                                                                                                                  <  1:478086/121‑1 (MQ=255)

                                                          agagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGc                                                                                <  1:908783/149‑1 (MQ=255)

                                                          agagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGACCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGc                                                                                >  1:687924/1‑149 (MQ=255)

                                                            agaAATATTGCACTTTATGTCGCGTGGCTACTCAATGACACAAATATCCGAGCAGCTTAAAAGCAATCTCCAAACGATCCGTGACCATAAATTTAATGTGATGTCGCAACTGGGCGTCCCTTATGACGAGGGGTTATTGGTGGCCGGAg                                                                              >  2:450131/1‑149 (MQ=255)

                                                                 tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATAtt                                                                         <  1:596197/149‑1 (MQ=255)

                                                                      cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTTAACGCAATATCAAAGCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                    >  1:925978/1‑149 (MQ=255)

                                                                      cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTTAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                    >  1:926386/1‑149 (MQ=255)

                                                                      cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                    <  2:311909/149‑1 (MQ=255)

                                                                      cgcTTTATCTCGCGTGGATACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                    <  1:897939/149‑1 (MQ=255)

                                                                            acgTCGCGTAGCTCCCTGATGACACAAAGTGCCGAGCATCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATTTGATGTCGAAACTCGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtat                                                              <  2:337979/147‑1 (MQ=255)

                                                                              gTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGc                                                            >  2:605444/1‑149 (MQ=255)

                                                                                cgcgTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCgg                                                          <  1:437184/149‑1 (MQ=255)

                                                                                    acatgaCTCAAATACACAAAGTGCCGAGCAGCTTAAACGCAATATCAAAACTATCCGTTAACATAAATTTAATGTGATGTCGAAACTGGGCGTTAGTTCTGACGCAGGGTTGTTGGATGCCGCAGATATTCTGTTATGTATGCGGCAtt                                                      <  2:557375/144‑1 (MQ=255)

                                                                                     ggCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTg                                                     <  1:282111/149‑1 (MQ=255)

                                                                                      gCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGc                                                    >  1:647446/1‑149 (MQ=255)

                                                                                         aCTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGAAGGGGGGTCGGACGCAGACGCTATGCTGTTATGTATGCGGCATTGCGag                                                 >  1:140418/1‑148 (MQ=255)

                                                                                          cTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAACGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGaac                                                >  1:989737/1‑148 (MQ=255)

                                                                                           tCAATGACACAAATTGCCGAGCAGCTTAAACGCAATCTCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCCGTTATGTATGCGGCATTGCGaacc                                               >  2:135676/1‑147 (MQ=255)

                                                                                           tCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc                                               >  1:1047863/1‑149 (MQ=255)

                                                                                           tCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGAGATTCTGTTATGTATGCGGCATTGCGAAAc                                               >  1:184485/1‑149 (MQ=255)

                                                                                             aaTGACACAAAGTGCCGAGCAGCTTAAAAGAAAAATCAAGTCGCTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGTTATTTGGGTATGTATGTGGCATTGTGAAACaa                                             <  2:481649/149‑1 (MQ=255)

                                                                                                gaaacaAATTGCCTGGCAGGTTAAATGAAATATCAAAAGGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTa                                          <  2:735724/146‑1 (MQ=255)

                                                                                                 acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATCTTCTGTTATGTATGCGGCATTGCGAAACAAGTaa                                         >  1:429440/1‑149 (MQ=255)

                                                                                                  cacaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                        <  2:202347/149‑1 (MQ=255)

                                                                                                  cacaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                        <  2:15636/149‑1 (MQ=255)

                                                                                                   taagaTTGCGGAGCCGCGTAAACTGAATATCAAAGCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg                                       <  2:214330/145‑1 (MQ=255)

                                                                                                   acaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAATTGGGCGTCGTTTCTGAGGCAGGGTTGTTTGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg                                       >  1:472936/1‑149 (MQ=255)

                                                                                                   acaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg                                       >  1:169160/1‑149 (MQ=255)

                                                                                                   acaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtg                                       >  2:826859/1‑149 (MQ=255)

                                                                                                     aaaTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgtg                                     <  2:939690/149‑1 (MQ=255)

                                                                                                     aaaTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTCAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgtg                                     >  2:937619/1‑149 (MQ=255)

                                                                                                      aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGATACGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATgtgt                                    >  2:178462/1‑149 (MQ=255)

                                                                                                           ccGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg                                                                                 <  2:626536/99‑1 (MQ=255)

                                                                                                           ccGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCg                                                                                 >  1:626536/1‑99 (MQ=255)

                                                                                                              agcagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGCGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATccc                            >  1:769999/1‑149 (MQ=255)

                                                                                                                 gggttAAACGCCATATGAAAACGATCAGTCAACATAAATTTAATGTGATGTGGAAACTGGGCGTCAGTTCTGACGCAGGGTTCTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTAt                         <  2:858579/146‑1 (MQ=255)

                                                                                                                   cTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTa                       >  1:273409/1‑149 (MQ=255)

                                                                                                                     tAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAATCAAGTAATGTGTTGCATCCCTATTAAt                     >  1:81221/1‑149 (MQ=255)

                                                                                                                      aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATc                    <  1:882438/149‑1 (MQ=255)

                                                                                                                        aCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCg                  >  1:587954/1‑149 (MQ=255)

                                                                                                                          gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCa                >  2:949434/1‑149 (MQ=255)

                                                                                                                          gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCa                <  2:687924/149‑1 (MQ=255)

                                                                                                                          gCAACAGCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGGATCCCTATTAATCCGCa                <  2:860989/149‑1 (MQ=255)

                                                                                                                             atatCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCatga             >  2:376158/1‑149 (MQ=255)

                                                                                                                             atatCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCatga             >  2:376364/1‑149 (MQ=255)

                                                                                                                                tCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGc          >  1:757446/1‑149 (MQ=255)

                                                                                                                                  aaaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCg        >  2:582534/1‑149 (MQ=255)

                                                                                                                                    aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCGTGATGCCggg      >  1:126783/1‑149 (MQ=255)

                                                                                                                                    aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCggg      >  1:476347/1‑149 (MQ=255)

                                                                                                                                        aTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa  <  2:630797/149‑1 (MQ=255)

CGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_pae_tpiA/4604489‑4604773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A