Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A22 F101 I1 R1 19 63.1 2283916 94.2% 2151448 141.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,604,632 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,604,6320CA98.5% 221.4 / NA 68A186E (GCA→GAA) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (33/34);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTACTT  >  NC_000913_3_pae_tpiA/4604486‑4604776
                                                                                                                                                  |                                                                                                                                                 
gcccgaacgaaTGAACAATCAGTGGCACCTTAAGCAAAACCGGACGTTAAGCCCGACGGAGAGGGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGaac                                                                                                                                                 <  1:210112/147‑1 (MQ=255)
           tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                      >  1:200087/1‑149 (MQ=255)
             aaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAtgt                                                                                                                                    >  2:169114/1‑149 (MQ=255)
               cAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGACAGAAATTCAGACGTGTATGTCGCGAGGTTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGa                                                                                                                                  <  1:835637/149‑1 (MQ=255)
                aaTCAGTGGTACATTAACCAAAGCCGGGCGTTAAGCCCGCGGGGGAGCGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCACATTACAAGCAATATCAAAACGAACCGTGAAAATAAATTTAATGTGAt                                                                                                                                 >  2:347307/1‑149 (MQ=255)
                aaTCAGTGGTACATTAACCAAAGCCGGACGTTCAGCCTAGGGGGTAGCGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCCGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAt                                                                                                                                 >  1:558301/1‑149 (MQ=255)
                   cAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTc                                                                                                                              <  1:36494/149‑1 (MQ=255)
                       ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGaaa                                                                                                                          <  1:811148/149‑1 (MQ=255)
                        gTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAAc                                                                                                                         <  1:261427/149‑1 (MQ=255)
                          aCATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTg                                                                                                                       <  1:424320/149‑1 (MQ=255)
                          aCATTAACCAAAGCCGGACGGTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTg                                                                                                                       <  1:435456/149‑1 (MQ=255)
                           cATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACtct                                                                                                                      >  2:268433/1‑147 (MQ=255)
                            aTTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTggg                                                                                                                     >  2:196173/1‑149 (MQ=255)
                             ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGc                                                                                                                    <  1:308288/149‑1 (MQ=255)
                             ttAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAAATGGGc                                                                                                                    >  1:478167/1‑149 (MQ=255)
                                 ccAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCa                                                                                                                <  2:335835/149‑1 (MQ=255)
                                  cAAAGCCCGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCATGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑g                                                                                                              <  1:1111488/149‑1 (MQ=255)
                                   aaaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAACTTTAATTTGATGTCGAAACTgggaggcggt                                                                                                              >  1:257535/1‑142 (MQ=255)
                                     gcccGGACGTTAAGTCCGACGGAGAGTGAAATATTGCGGTTTATGTGGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTc                                                                                                           <  2:938795/147‑1 (MQ=255)
                                           aggtgtggCCCGACGGAGAGAGAAAGAGTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAACCTGGGCGTCA‑GTTCTGACGc                                                                                                     <  2:232832/142‑1 (MQ=255)
                                              ttAAGGCGGTCGGAGTGTGTAATGTTGCGCTTTATGTCGCGTGGTTACTCAATGACACAAATTGCCTAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATATATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAgg                                                                                                  <  2:323256/149‑1 (MQ=255)
                                               tAAGCCCTACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAggg                                                                                                 >  2:723266/1‑149 (MQ=255)
                                                    ccGACGGGGAGAGAAATATTGCGCTTTATGTGGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTAACTTTTTAACTGGGCGTCA‑GTTCTGACGCAGGgttgtt                                                                                            <  2:245361/149‑1 (MQ=255)
                                                           agagagaAATATTGCGCTTTATGTAGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAATGCAATATCAAGACGATCCGTGAACATAAATTTAATGTGGTGTCGAAACTGGGCGTAC‑GTTCTTATGCAGGGTTGTTGGAGGcc                                                                                     >  2:631711/1‑149 (MQ=255)
                                                            gagagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCg                                                                                    <  2:428542/149‑1 (MQ=255)
                                                               agaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTCGTTGGAGGCCGccg                                                                                 >  1:251282/1‑147 (MQ=255)
                                                                gaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGa                                                                                <  1:169114/149‑1 (MQ=255)
                                                                 aaaTATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCG‑GTTCTGACCCAGGGTGGTTGGAGGCCGCAGat                                                                               >  1:917664/1‑149 (MQ=255)
                                                                     atTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGGGGCCGCAGGTATTc                                                                           >  2:489315/1‑149 (MQ=255)
                                                                         cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                       <  2:478167/149‑1 (MQ=255)
                                                                         cgcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGtt                                                                       <  1:999955/149‑1 (MQ=255)
                                                                            tttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTCAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTTGGCGTCA‑GTTCTGACGCAGGGTTGTTGGCGGCACCAGATATTTTGTtatg                                                                    >  2:171244/1‑149 (MQ=255)
                                                                            tttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTc                                                                           <  1:326413/142‑1 (MQ=255)
                                                                            tttATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTc                                                                           >  2:326413/1‑142 (MQ=255)
                                                                              tATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgta                                                                  >  2:151524/1‑149 (MQ=255)
                                                                                 gTCGTGTGGCTACTCAATGACAAAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGc                                                               <  2:97801/149‑1 (MQ=255)
                                                                                 gTCGCGTGGCTACTCAATGACCCAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGc                                                               <  1:719468/149‑1 (MQ=255)
                                                                                      gTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGGCGCAGGGTTGTTGGGGGCCGCAGATTTTGTGTTATGTATGTgggtt                                                          >  1:131941/1‑146 (MQ=255)
                                                                                      gTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAt                                                          >  2:778373/1‑149 (MQ=255)
                                                                                       tGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCAtt                                                         <  2:562487/149‑1 (MQ=255)
                                                                                         gCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGc                                                       <  1:910469/149‑1 (MQ=255)
                                                                                                aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa                                                >  1:824011/1‑149 (MQ=255)
                                                                                                  tGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCCGATATTCTGTTATGTATGCGGCATTGCGAAACAAGt                                              >  1:806099/1‑149 (MQ=255)
                                                                                                  tGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGCTATTCTGTTATGTATGCGGCATTGCGAAACAAGt                                              >  1:186845/1‑149 (MQ=255)
                                                                                                    acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGATGTTGGAGGCCGCAGATATTCTGTTATGTATGCGCCATTGCGAAACAAGTaa                                            >  2:375287/1‑149 (MQ=255)
                                                                                                     cacaAATTGCCAAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                           >  2:1069629/1‑149 (MQ=255)
                                                                                                       caAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGAAC‑GTGTGGACCCATGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgt                                         <  1:787846/149‑1 (MQ=255)
                                                                                                       caAATGTCCGAGCAGCTTAATCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTGA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAtgt                                         <  2:954631/149‑1 (MQ=255)
                                                                                                         aaTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCCGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATgtgt                                       >  2:258226/1‑149 (MQ=255)
                                                                                                          aTTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                      >  1:945081/1‑149 (MQ=255)
                                                                                                                tagcaacTTAAACGCAATATCAAAATGATACGTGAAAATAATGTGGAGCTGATTGCGAAACTGGGCGTCA‑GTTCTGCTGCAGGGTTGTTGTAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATcc                                <  2:690593/148‑1 (MQ=255)
                                                                                                                 agcagcTTAAACGCCATAACAGAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATccc                               <  1:593868/149‑1 (MQ=255)
                                                                                                                   cagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTa                             <  2:956313/149‑1 (MQ=255)
                                                                                                                    agcTTAAACGCAACATCAAAACGATCCGTTAACATAAATTTACTGCGATGGCATAGCGGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGGATATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTAt                            <  1:723266/149‑1 (MQ=255)
                                                                                                                       ttAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTaa                         >  1:952759/1‑149 (MQ=255)
                                                                                                                          aaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGgttgtt                                                                                            >  1:1028874/1‑79 (MQ=255)
                                                                                                                          aaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGgttgtt                                                                                            <  2:1028874/79‑1 (MQ=255)
                                                                                                                             gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGAAGGGTTGTTGGAGGCCGCAGGTATTCTCTTATGTATGCGGCCTTGCGAAACAAGTACTGCATTGCATCCCTCTTAAACCgac                   >  2:1034783/1‑147 (MQ=255)
                                                                                                                              gaaTATCAAAACGGTCCTTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                  <  2:53876/148‑1 (MQ=255)
                                                                                                                                  atCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGGCGCGAATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCatgatg              >  2:695886/1‑149 (MQ=255)
                                                                                                                                       aaCGATCCGTGAACATGTGTTTGGTGTGATGTCGAAAC‑CGNCACCATGTGCTGACGCGGGACTGTCGGGTTCGTGAGATGGTCGGTGATGTATGCGGCATTGCGAAACAAGTAANGTGTTGCATCCCTATTAATCCGCATGATGCCggg         >  2:1096299/1‑149 (MQ=255)
                                                                                                                                       aaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCggg         >  2:799614/1‑149 (MQ=255)
                                                                                                                                        accctCCTTGAACATAAATTTAATGTGATGGCGAAACTGGGCGTTA‑GTTCTGACGCAGGGTTGTTGGAGGCCGAAGATATTCTGTTATGTATTCTGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGt        <  1:171244/145‑1 (MQ=255)
                                                                                                                                         cGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGtt       >  2:440388/1‑149 (MQ=255)
                                                                                                                                           acccGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa     <  1:456254/147‑1 (MQ=255)
                                                                                                                                              cGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTGATGTGTTGCATTCCTATTAATCCGCATGATGCCGGGTTTACtt  >  2:1066176/1‑149 (MQ=255)
                                                                                                                                              cGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTACtt  <  1:1139174/149‑1 (MQ=255)
                                                                                                                                               gTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                  >  2:663433/1‑132 (MQ=255)
                                                                                                                                               gTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat                  <  1:663433/132‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCA‑GTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTACTT  >  NC_000913_3_pae_tpiA/4604486‑4604776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A