Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F19 I0 R1 34 56.7 1876034 94.8% 1778480 147.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_tpiA 4,604,632 C→A 100% A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,604,6320CA100.0% 234.1 / ‑6.7 74A186E (GCA→GAA) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (39/34);  minor base G (1/0);  total (40/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_pae_tpiA/4604492‑4604773
                                                                                                                                             |                                                                                                                                             
ccgacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAt                                                                                                                                       >  1:757183/1‑149 (MQ=255)
  gacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAAAAAAATATAGAGCTTAATGTCGCGTAGCTACTCAATAACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAttt                                                                                                                                     >  1:371985/1‑149 (MQ=255)
   acTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTa                                                                                                                                    >  2:168844/1‑149 (MQ=255)
    cTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATCAATTTaa                                                                                                                                   <  1:671353/149‑1 (MQ=255)
    cTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTaa                                                                                                                                   <  2:722675/149‑1 (MQ=255)
     tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                  >  2:758107/1‑149 (MQ=255)
     gtaaCAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                  <  1:846438/147‑1 (MQ=255)
         cAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGa                                                                                                                              <  2:557508/149‑1 (MQ=255)
          aaTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAt                                                                                                                             >  2:346631/1‑149 (MQ=255)
          aaTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATAACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAAAGATCCGTGAACATAAATTTAATGTGAt                                                                                                                             >  1:922073/1‑149 (MQ=255)
                tGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATACATTTAATGTGATGTCGaa                                                                                                                       >  2:528696/1‑149 (MQ=255)
                 ggTACATTAACCAAAGCCGGACGTTAAGTCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGaaa                                                                                                                      <  1:149321/149‑1 (MQ=255)
                 ggTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGaaa                                                                                                                      <  1:260823/149‑1 (MQ=255)
                         aaCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGt                                                                                                              <  1:529287/149‑1 (MQ=255)
                            cAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAg                                                                                                           >  1:552141/1‑149 (MQ=255)
                             aaaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCaat                                                                                                          >  2:585167/1‑147 (MQ=255)
                             aaaGCCGGACGTTAAGCACGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCGACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGt                                                                                                          <  1:608614/149‑1 (MQ=255)
                              aaGCCGGACGTTACGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTTGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt                                                                                                         <  1:513826/149‑1 (MQ=255)
                              aaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGtt                                                                                                         <  1:462108/149‑1 (MQ=255)
                              aaGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGAGAACATAAATGTAATGTGATGTCGAAACTGGGCGTCAGtt                                                                                                         >  1:414301/1‑149 (MQ=255)
                                 ccGGACTTTAAACCTGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg                                                                                                      <  2:734951/149‑1 (MQ=255)
                                 ccGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGACGAGCAGCTTAAACGCAATCTCAAAACGATCCGTGAAAATAAATTTAAGGTGATGTGGAAACTGCGCCTCAGATCtc                                                                                                      >  2:721242/1‑148 (MQ=255)
                                  cGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTCCtca                                                                                                     >  1:577979/1‑147 (MQ=255)
                                    gACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACg                                                                                                   >  2:427181/1‑149 (MQ=255)
                                       gTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAGACTGGGCGTCAGTTCTGACGCAg                                                                                                <  1:808473/149‑1 (MQ=255)
                                         tAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTATGACGCAggg                                                                                              >  2:90470/1‑149 (MQ=255)
                                           aGCCCGACGGAGAGAGAAATATTGCGCTTTAGGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgtt                                                                                            <  2:212615/149‑1 (MQ=255)
                                              ccGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt                                                                                         <  2:832795/149‑1 (MQ=255)
                                              ccGACGGAGAGAGAAATATTACGCTTTATGTCGCGTGGCTACTCAATGACACCCCCGTGATG‑GCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgttgtt                                                                                         <  1:681404/149‑1 (MQ=255)
                                               cGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATTGGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAGGCCGGGctgttg                                                                                        >  1:776144/1‑149 (MQ=255)
                                                 aCGGAGAGACAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTggc                                                                                      <  2:135948/149‑2 (MQ=255)
                                                        gagaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGGACATAAATTTACTGTGATGTCGAAACTGGGCGTCAGTTCTGACTCAGGGTTGTTGGAGACCGCa                                                                               >  2:140739/1‑149 (MQ=255)
                                                         agaAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGAAcgccgccg                                                                              >  2:890029/1‑147 (MQ=255)
                                                           aaaTATTGCGCTTTATGTCGCTTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTTGGCGTCAGTTCTTACGCAGGGTTGTTGGAGAcagcaggt                                                                            >  2:759250/1‑147 (MQ=255)
                                                            aaTATTGCGCTTTATGGCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGata                                                                           <  2:724980/149‑1 (MQ=255)
                                                                ttGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGATCAGCTTAAACGCAATAGCAAAACGATCCGTGAACATAAATGTAATTTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt                                                                       <  2:49390/149‑1 (MQ=255)
                                                                ttGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAACTTTTGTTTCATCTCGACACTGGGTGGCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt                                                                       <  1:606184/149‑1 (MQ=255)
                                                                ttGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCt                                                                       <  1:778230/149‑1 (MQ=255)
                                                                    gcTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGAAGCAGGGTTGTTGGAGGCCGCAGATATTCTGTta                                                                   >  2:439475/1‑149 (MQ=255)
                                                                       ttATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCGGATATTCTGTtatgt                                                                >  1:767063/1‑149 (MQ=255)
                                                                         aTGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtatgtat                                                              >  1:865366/1‑149 (MQ=255)
                                                                           gTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGTAATAGAAAAACGATCAGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTATGAGGGAGGGTAATTGGAGGCAGCAGATATACGGTTATGTATGc                                                            >  2:503767/1‑149 (MQ=255)
                                                                              gcgTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGc                                                         >  1:102526/1‑149 (MQ=255)
                                                                               cgTGGCTTCTAAATGACA‑CAAATCGCCGAGCAGACTAAACGAAAGATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTTGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCa                                                        <  1:120693/149‑1 (MQ=255)
                                                                                     tACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGa                                                  <  1:104659/149‑1 (MQ=255)
                                                                                     tACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGCCAGTTCTGACGCAGGGTTGTTGGAGGCCGCCGATCATATGTTCTGCATCCGGCATTGCGa                                                  >  2:835319/1‑149 (MQ=255)
                                                                                       ctaaaTGACA‑CAAATTGCCGAGCAGCTTAAACGCAATACCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGaaa                                                <  2:53901/146‑1 (MQ=255)
                                                                                        tCAATGGCA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAGTGAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGGTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAAc                                               >  2:203187/1‑149 (MQ=255)
                                                                                        tCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAGAc                                               >  2:910062/1‑149 (MQ=255)
                                                                                         aaaTGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACa                                              >  1:514234/2‑149 (MQ=255)
                                                                                          aaTGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGATTGTTGGAGGCCGCAGATATTCTGTTATGTACGCGGCATTGCGAAACaa                                             >  2:623479/1‑149 (MQ=255)
                                                                                           aTGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAg                                            >  2:904580/1‑149 (MQ=255)
                                                                                               ca‑ccAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAAt                                        <  2:796763/149‑1 (MQ=255)
                                                                                                a‑caAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATTCGGCATTGCGAAACAAGTCAtg                                       >  1:153405/1‑149 (MQ=255)
                                                                                                  caAATTGCCGAGCAGCGTAAACGCAATATCAAAACGATCAGAGAACATAAATTTAATGTAATGTCGAAAATGGGCGACAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGCTACTGTGTTAGGTATGCGGCGTTGCGAACAAAGTAAtgt                                      >  2:193642/1‑149 (MQ=255)
                                                                                                    aaTTGCTCAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGAGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATgtgt                                    <  2:185949/149‑1 (MQ=255)
                                                                                                     actGCCGAGCAGCTTACACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                   <  1:799938/147‑1 (MQ=255)
                                                                                                     aTTGCCGGCCAGTTTAAACGGAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTTTCATGTACGCTGCATTGCGAAACAAGTAATGTGtt                                   <  2:487807/149‑1 (MQ=255)
                                                                                                      ttGCCGAGCAGCTTAAACGCAATATCAAAGCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGGTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTg                                  <  1:9112/149‑1 (MQ=255)
                                                                                                           gggcagcTTGAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATcc                             <  2:776756/147‑1 (MQ=255)
                                                                                                           gAGTAGCTTAAACGAAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATcc                             <  2:922073/149‑1 (MQ=255)
                                                                                                           gAGCCGCTTAAACGCAAGACCAATACGATCCTTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGATGCAGGGTTGTTGGAAGCCGCATATCTTCTGCTATGTACGCGGCATTGCGAAACAAGTAATGTGTTGCATcc                             <  2:579194/149‑1 (MQ=255)
                                                                                                             gcagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGGATGCGGCATTGCGGAACAAGTAATGGGTTGCATCCCt                           >  1:389324/1‑149 (MQ=255)
                                                                                                              cagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTa                          <  2:600313/149‑1 (MQ=255)
                                                                                                                 cTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTAGGCATCAGTTCTGACACAGGGTTGTGGGAGGCCGCAGATATCACGTTATGTATGCGGCCTTGCGACAAAAGTAATGTGTTGCTACCCtgtaa                       >  2:263918/1‑145 (MQ=255)
                                                                                                                      aCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCTGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATcgg                  >  2:295317/1‑147 (MQ=255)
                                                                                                                      aCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTCATGTGTTGCATCCCTATTAATCCg                  >  2:295431/1‑149 (MQ=255)
                                                                                                                        gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCa                <  1:346631/149‑1 (MQ=255)
                                                                                                                        gCAATATCAAAACGATCCGTGAACATAAACTTAATGTCATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTANTGTGTTGCATCCCTATTAATCCGCa                >  1:619728/1‑149 (MQ=255)
                                                                                                                         cAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCat               <  1:368769/149‑1 (MQ=255)
                                                                                                                              tCAAAACAATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGc          >  1:668505/1‑149 (MQ=255)
                                                                                                                                aaaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCg        >  1:377397/1‑149 (MQ=255)
                                                                                                                                aaaaCGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGACg        >  2:890873/1‑149 (MQ=255)
                                                                                                                                      aTCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa  >  1:600291/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                             
CCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACA‑CAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_pae_tpiA/4604492‑4604773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A