Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F36 I0 R1 271 6613.7 10404966 98.5% 10248891 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.97.2% 938.8 / 17.8 211intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base . (95/110);  total (99/112)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 211 total aligned reads displayed.

CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870777‑4871047
                                                                                                                                       |                                                                                                                                       
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                     >  3:449492/1‑140 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACTGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  >  7:205404/1‑140 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  1:1129347/140‑1 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  3:673869/140‑1 (MQ=255)
   gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                                 >  2:1203394/1‑140 (MQ=255)
   gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                                 >  8:834985/1‑140 (MQ=255)
       atcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCAAGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGtt                                                                                                                             <  8:640003/140‑1 (MQ=255)
        tcatTTCTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                            <  8:796794/140‑1 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttta                                                                                                                           >  3:1238758/1‑139 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  4:1078313/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGAATGGATGATGTTCAACGACACGGCGACCACTGACTGCAGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  2:592277/1‑140 (MQ=255)
          atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          <  1:936821/140‑1 (MQ=255)
              ttCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATTGATGATGCTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  4:1128241/139‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCCTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      >  6:1232087/1‑140 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  7:1243961/140‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      >  3:59071/1‑140 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      >  4:308650/1‑140 (MQ=255)
              aTCAATTTTTTCATTATTATGCTCCAGCAATTACCGTAGGGAATGGATGATTATAAACGACACGGCTAACAAAGACTGCCGAAGAATCCATTGTGCATCAGTCAAGGGAAAAGAATATTCG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  7:602182/140‑1 (MQ=255)
                aaaTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGa                                                                                                                    <  6:174677/139‑1 (MQ=255)
                 aaTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaa                                                                                                                   >  2:1121326/1‑140 (MQ=255)
                 aaTTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaa                                                                                                                   >  1:177557/1‑140 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  1:36523/1‑140 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  2:272642/140‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  3:425743/140‑1 (MQ=255)
                       ttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACatat                                                                                                             <  6:525817/140‑1 (MQ=255)
                             attTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTa                                                                                                       <  6:351423/140‑1 (MQ=255)
                                 tGCTCCATCAATTACTGTAGGGCATGGATGATGTTCAAAGACACGGCGAACACTGACTGCCGATGAATCCATTGTGCATCAGGCAATGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  4:683693/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  3:851748/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  1:1163479/140‑1 (MQ=255)
                                   cTCAAGCAATTACGGTAGGGCATGGATGATGTTAAACGACACGGCGACCAATGACTGCCGATGAATCCTTTGTGCATCAGGCTAGGTAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatgaat                                                                                                 <  4:80555/140‑1 (MQ=255)
                                        gCATTTACGGTAGGGCATGGATGATGTGCAACGACAGGGCGACCACTGACTGCCGATGAATCCATTGTGAATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGAGAAACTTATGCTTTAATGAATGTTcc                                                                                            <  7:1202969/140‑1 (MQ=255)
                                                    gggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtt                                                                                >  2:1291864/1‑140 (MQ=255)
                                                         tGGATGATGTTCACCGACACGGCGACCACTGACTGCAGATGAATCCATTTTGCATCAGGCTAGTGAAAAGAAAATTAG‑ACAAATTAGGTTTCATAGTGATACTGATGCTATAATGAATGTTCCATATTGAAACTTTTACg                                                                           >  3:1062132/1‑140 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  3:19758/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  2:868453/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  7:434810/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  2:553977/140‑1 (MQ=255)
                                                          ggaatatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTa                                                                                                       >  8:442785/6‑111 (MQ=255)
                                                            atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                        >  6:743686/1‑140 (MQ=255)
                                                               atgTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAACCATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  2:423527/1‑140 (MQ=255)
                                                               atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  1:45545/1‑140 (MQ=255)
                                                                tgtgCAACGACACGGCGACTACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCATTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    <  7:917210/140‑1 (MQ=255)
                                                                tgTTCACCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGACAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    <  7:819040/140‑1 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTTTGCATCAGGCAAGTTAAAAGAAAATTAG‑CCAAAGTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATAATGAAACTTTTACGTGTATTa                                                                    <  4:128335/140‑1 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  2:503646/1‑140 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  4:1174395/1‑140 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    <  4:369309/140‑1 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  4:573085/1‑140 (MQ=255)
                                                                 gTTCAACTACACGGAGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAATGGATAAGAAAAGTAG‑CAAAATTAGTTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTAAGTGTATTaa                                                                   >  8:711409/1‑140 (MQ=255)
                                                                     aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  5:1121112/1‑140 (MQ=255)
                                                                     aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  3:395592/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          <  6:269536/76‑1 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          >  5:269536/1‑76 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  5:919107/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  1:1172879/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  7:835926/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  7:1112349/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  2:259344/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  3:792848/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  7:164285/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  3:1323214/1‑140 (MQ=255)
                                                                             ggCGACCACTGACTGCCGATAAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTg                                                       <  3:733409/140‑1 (MQ=255)
                                                                               cGACCACTGACTGCCGATGAATCCATTGTGCCTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                     <  7:1140418/140‑1 (MQ=255)
                                                                                 aCCACTGACTGCCGATGAATCCATTGTGCCTCAGGCAAGGGAAATGAAAATTAG‑CCTAATAAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                   >  3:550250/1‑140 (MQ=255)
                                                                                 aCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                   <  4:141704/140‑1 (MQ=255)
                                                                                  ccACTGCATTCCGATGACTCCATTGTTCAGCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTAATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAACCTTTTACGTGTATTAATACTTAAAATTGCGAGc                                                  <  6:947502/140‑1 (MQ=255)
                                                                                       gtctgCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                             <  5:1190643/138‑1 (MQ=255)
                                                                                            ccGATGAATCCATTGTGCTTCAGGCAAGGGAAAAGAAAATAAG‑CAAAATTAGCTTTCATTGTGAAAAATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGAGTATTAATACTTAAAATTGAGAGCCGGAACAccg                                        >  8:152911/1‑139 (MQ=255)
                                                                                             ctattaaTACATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCTAATTAGTTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  2:952668/135‑1 (MQ=255)
                                                                                             ctaTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTGTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       >  5:768195/3‑140 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       >  4:875379/1‑140 (MQ=255)
                                                                                              gATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCttt                                      <  1:812453/140‑1 (MQ=255)
                                                                                               aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               >  7:1120208/1‑66 (MQ=255)
                                                                                                    tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                >  1:1204077/1‑140 (MQ=255)
                                                                                                     ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAAGTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                               <  5:1244777/140‑1 (MQ=255)
                                                                                                     ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                               <  2:45545/140‑1 (MQ=255)
                                                                                                           tgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGAt                         >  5:995423/1‑140 (MQ=255)
                                                                                                            tcagcAGGCAAGGGAAAAGAAATTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCTTATTGATACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  6:770907/136‑1 (MQ=255)
                                                                                                            tcATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTACATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:1337845/139‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGTGAATAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTGCATATTGAATCTTTTTCGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:10491/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAATTAAATTA‑TCCAAATTTCGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATTCTTAAAATTTCGAGCCGGTACACCTTTTTTCATAAGGGATg                        <  5:1012933/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGaag                        <  7:505115/140‑3 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:885829/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:800247/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATAAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  8:884667/140‑1 (MQ=255)
                                                                                                             cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACAGATGCTTTAATGAAGGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTCTCATAAGGGATGc                       >  2:979568/1‑140 (MQ=255)
                                                                                                              aTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCg                      >  2:225002/1‑140 (MQ=255)
                                                                                                                cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                    >  4:753443/1‑140 (MQ=255)
                                                                                                                  ggCAAGGGAAACGAACATTA‑CCCAAATTAGGTTTCATAGTGAACCATAGGCTTTAATGTATGTTCCATATTGTAACTTTTACGTGTATTAATTCTTAAAATTGCGAGCCGTAACACCTTTTGTAATAAGGGATGCGGGat                  <  7:285451/140‑1 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATGGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGTTGCGGGat                  >  8:333814/1‑140 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                  <  7:754441/140‑1 (MQ=255)
                                                                                                                         taaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGATCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  8:381740/139‑1 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTTAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  6:504799/140‑1 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  3:1164290/140‑1 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  1:777450/140‑1 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  1:1291864/140‑1 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  5:520054/140‑1 (MQ=255)
                                                                                                                          aaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTgg          <  2:724353/140‑1 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGACCACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg        >  2:1005570/1‑140 (MQ=255)
                                                                                                                               aaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCGTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtt     <  1:1191749/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870777‑4871047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A