Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F2 I0 R1 184 7301.6 8720326 98.4% 8580800 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.95.4% 820.8 / 24.0 190intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base . (87/94);  total (93/97)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 191 total aligned reads displayed.

GTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGC  >  NZ_CP022686/4870775‑4871042
                                                                                                                                         |                                                                                                                                  
gTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcca                                                                                                                                  <  1:515432/140‑2 (MQ=255)
  cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                >  3:358511/1‑140 (MQ=255)
  cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                >  3:285708/1‑140 (MQ=255)
  cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                >  4:774599/1‑140 (MQ=255)
    tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                             <  2:560406/140‑1 (MQ=255)
    tGCACATCAATGATCAATTTTTTCATTATTTTGCTCCAGAAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAATGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                             <  4:986616/140‑1 (MQ=255)
      cacaTCATTGATCAATTTTTTCATTATTTTTCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGTAAAAGAAAATTAG‑CCAAATTAg                                                                                                                           >  3:959646/1‑140 (MQ=255)
      cacaTCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAg                                                                                                                           >  1:446931/1‑140 (MQ=255)
          tcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGt                                                                                                                         <  5:571708/138‑1 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                      >  2:399886/1‑140 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGAATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttta                                                                                                                      >  4:258162/1‑139 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATATTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                      >  2:372616/1‑140 (MQ=255)
            atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                     <  3:388501/140‑1 (MQ=255)
               gATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCAt                                                                                                                    >  5:494611/1‑138 (MQ=255)
                agcAATTTTTTCTTTATTTTGCTCCAGCAATTACGGTATTGCAGGGATGATGTGCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCTAATTAGGTTTCATAGt                                                                                                                 <  8:278981/138‑1 (MQ=255)
                aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                 <  1:289482/140‑1 (MQ=255)
                aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                 >  1:439965/1‑140 (MQ=255)
                aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAATAGGTTTCATAGt                                                                                                                 >  2:701147/1‑140 (MQ=255)
                aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGGCGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                 <  8:150183/140‑1 (MQ=255)
                 tCAATTTTATCATTATTTTGCTCCAGCATTTACGGTAGGGCATGGATGTTGTTCAACGACACGGCGCCCACTGACTGACGATGAATCCATTGTGCATCAGGCAATGGAAAAGAAAACTAG‑CCAAATTAGGTTTCATAg                                                                                                                  <  6:235694/138‑1 (MQ=255)
                  cAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGa                                                                                                               <  3:999780/140‑1 (MQ=255)
                     ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGAGACCACAGACTGCCGATCAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                            >  2:95758/1‑140 (MQ=255)
                     ttttttCATTATTGTGCTCCAGCAATTACGGTAGGGAATGGATGATCTTCAACGACACGGCGACAACTTACTTCCGTTGACTCCATTGTGCATAAGGCAAGGGAAAAGAAAATAAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                            >  8:128235/1‑140 (MQ=255)
                       ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                          <  7:426377/140‑1 (MQ=255)
                       ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                          <  3:227770/140‑1 (MQ=255)
                       ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTGCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                          <  8:598438/140‑1 (MQ=255)
                       ttttAATTATTTTGCTCCAGTAATTTCGTTATTGCTTGGAGGATTTTCAACGACACGGCGACCACTGACTGCCGATGAATCCCTTGTGCATCAGGAAATGGAAAAGAAAATTA‑TCCAAATTTGGTTGCATAGTGaaaa                                                                                                            <  6:1037299/138‑2 (MQ=255)
                            attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAGCGACACGGCGACCACTGCCTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAGCATATg                                                                                                       >  6:382943/1‑138 (MQ=255)
                              tattTTGCGCCAGCAATTACGGTAGGGAATGGATGATGTTCAACGAGACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCttt                                                                                                   <  8:464968/140‑1 (MQ=255)
                              tattTTCCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCttt                                                                                                   <  4:663141/140‑1 (MQ=255)
                               attTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACAAGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTa                                                                                                  <  1:798660/140‑1 (MQ=255)
                                 tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTa                                                                                                  <  5:871386/138‑1 (MQ=255)
                                   tGCTCCAGCAATTACGGTAGGTCAAGGATGATGTTCAACGACACTGCTAACACTGACTGCCTATTAATACATTTTGCATCAGTGAAGGGAAAAGAAAATTA‑TACAAATTATGTTTCATAGTGAAACATATGCTTTtatta                                                                                              <  4:540795/140‑3 (MQ=255)
                                   tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                              >  1:482052/1‑140 (MQ=255)
                                   tGCTCCACCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                              <  2:227187/140‑1 (MQ=255)
                                                aCGGTCGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCatat                                                                                   <  5:449916/138‑1 (MQ=255)
                                                aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                 <  2:894111/140‑1 (MQ=255)
                                                aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                 <  8:711520/140‑1 (MQ=255)
                                                        gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtttt                                                                         >  3:780517/1‑140 (MQ=255)
                                                        gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCGTTAATGAATGTTCCATATTGAAACtttt                                                                         >  3:69985/1‑140 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTTACTGCCGATGAATCCATTGTGCATCAGTCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGATACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                      <  1:1037561/140‑1 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                      <  3:660300/140‑1 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                      >  8:56095/1‑140 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATAAG‑CCAAATTAGGATTCATAGTGAAACATATGCTTAAATGAATGTTCCATATTGAAACTTTTACg                                                                      <  1:369286/140‑1 (MQ=255)
                                                           tGGAAGATGTTCAACGACACGGCGACCACTGACTGCCGAAGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                      <  4:470839/140‑1 (MQ=255)
                                                           gggATGATGTGCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                      <  8:402619/139‑1 (MQ=255)
                                                            ggATGATTTTCAACGACACGGAGAACACAGACTTACGCTCAATCAATTGAGAATAAGTAAACGGAAAAGAAAATTA‑TCCAAATAATGATTCATAGTCAAACAAATGCATTAATGAATGTTCCATATTCAAAATTTAActt                                                                     >  8:877826/1‑138 (MQ=255)
                                                            ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                     >  2:353395/1‑140 (MQ=255)
                                                            ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                     >  4:386227/1‑140 (MQ=255)
                                                              atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                 >  8:258175/1‑94 (MQ=255)
                                                              atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                 <  7:258175/94‑1 (MQ=255)
                                                                 atgTTCAACGACACGGCGCACACTGACTCCCGATGCTGACATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGACACATATGCTTAAATGAATGTTCCATATTGAAACTATTACGTGTagt                                                                >  1:621055/1‑138 (MQ=255)
                                                                 atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                >  7:527113/1‑140 (MQ=255)
                                                                 atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAAACTTTTACGTGTAtt                                                                >  4:279722/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATg                                                                                                       >  1:475881/1‑100 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                               >  1:846093/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                               >  3:556273/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                               >  2:503447/1‑140 (MQ=255)
                                                                   gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGGATTaa                                                              >  1:708431/1‑140 (MQ=255)
                                                                    ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                               <  5:526780/138‑1 (MQ=255)
                                                                    ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGATTTAATGAATGTTCCATATTGAAACTTTTAAGTGTATTa                                                               >  5:23986/1‑138 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                     >  1:1022810/1‑76 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATTTGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                     >  8:474203/1‑140 (MQ=255)
                                                                              cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAAGGTTCAATATTGAAACTTTTACGTGTATTAATAATTAAAAtt                                                   >  4:842154/1‑140 (MQ=255)
                                                                               ggCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTg                                                  <  7:372717/140‑1 (MQ=255)
                                                                                 cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTg                                                  <  5:360400/138‑1 (MQ=255)
                                                                                 cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                <  2:846093/140‑1 (MQ=255)
                                                                                   aCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                              >  1:263241/1‑140 (MQ=255)
                                                                                       ctgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCgg                                          <  2:355022/140‑1 (MQ=255)
                                                                                         gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                        <  1:596292/140‑1 (MQ=255)
                                                                                          actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAGCTTTTACGTGTATTAATACTTAAAATTGAGAGCCGGAac                                       >  2:790623/1‑140 (MQ=255)
                                                                                              ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATTAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                   >  2:757991/1‑140 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                  <  3:177752/140‑1 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                  <  1:129478/140‑1 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                  >  2:304859/1‑140 (MQ=255)
                                                                                                gATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCttt                                 <  4:82910/140‑1 (MQ=255)
                                                                                                      tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                     <  2:2689/50‑1 (MQ=255)
                                                                                                       ccATTGGGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                          <  2:150578/140‑1 (MQ=255)
                                                                                                        cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATTCTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                         >  7:899270/1‑140 (MQ=255)
                                                                                                        cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                           >  6:72118/1‑138 (MQ=255)
                                                                                                        cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                           >  5:402830/1‑138 (MQ=255)
                                                                                                        cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                         >  2:649991/1‑140 (MQ=255)
                                                                                                            gtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTATAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                     <  3:125821/140‑1 (MQ=255)
                                                                                                            gtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCTGGAACACCTTTTGTCATAAGGGa                     >  1:955734/1‑140 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATTCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                   <  3:419920/140‑1 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                     <  6:494611/138‑1 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                     <  5:657222/138‑1 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑ACAAATTAGGTTTCATAGGGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                     <  6:972895/138‑1 (MQ=255)
                                                                                                                    ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat             >  8:738384/1‑140 (MQ=255)
                                                                                                                    ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGATTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACTTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat             >  3:448660/1‑140 (MQ=255)
                                                                                                                     gCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGata            >  4:945730/1‑140 (MQ=255)
                                                                                                                      cAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat             >  6:654152/1‑138 (MQ=255)
                                                                                                                       aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGatatt          >  7:85598/1‑139 (MQ=255)
                                                                                                                       aaGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATg          >  4:177808/1‑140 (MQ=255)
                                                                                                                         gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg        >  7:202995/1‑140 (MQ=255)
                                                                                                                          ggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGt       >  2:836995/1‑140 (MQ=255)
                                                                                                                            aaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACCCCTTTTGTCATAAGGGATGCGGGATATGAGt       >  6:578817/1‑138 (MQ=255)
                                                                                                                              aagaaaaTTAG‑CCAATATAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACATTTTGTCATAATGGATGCGGGATATGAGTGgcg   >  3:753925/1‑140 (MQ=255)
                                                                                                                              aagaaaaTTAG‑CCAAATTAGTTTTCATAGTGAAACATATGATTTAATGAATGTTCCATATTGAAACTTTTACGTGTAGTAATACTTAACATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCTGGATATGAGAGgcg   >  1:999172/1‑140 (MQ=255)
                                                                                                                              aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTgg     <  5:769277/138‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
GTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGC  >  NZ_CP022686/4870775‑4871042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A