Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F2 I0 R1 130 5456.6 6845820 98.4% 6736286 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.96.4% 592.9 / 8.5 136intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base . (65/66);  total (67/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 136 total aligned reads displayed.

CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870777‑4871047
                                                                                                                                       |                                                                                                                                       
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                     >  7:327732/1‑140 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGTGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  >  3:653054/1‑140 (MQ=255)
  tGCACAGCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGTGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCCTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  6:333175/140‑1 (MQ=255)
        tcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                            <  5:838115/140‑1 (MQ=255)
        tcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                            <  8:5303/140‑1 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCTTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  4:240511/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  3:403120/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  6:559118/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATATTTCTAAATCAATTAAGGTAGGTCTTTGATGATGTTAAACGACAAGGATACCACTTACTGCCGATGAATCCATTGTTCAACAGGCAAGCGCAAAGAAAATTAG‑CCAAATTAGCttta                                                                                                                           >  8:126710/1‑139 (MQ=255)
         catTGATCAAATTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATAAG‑CCAAATTAGGTTTc                                                                                                                           >  4:548202/1‑140 (MQ=255)
          atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          <  8:447185/140‑1 (MQ=255)
            tGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGAAACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                        >  5:600412/1‑140 (MQ=255)
             taTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGGAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAg                                                                                                                       <  6:806135/139‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  5:512403/140‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      >  2:761588/1‑140 (MQ=255)
                 aaTTTTTTCATTATTATGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaa                                                                                                                   <  7:481338/140‑1 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGaaaa                                                                                                                 >  4:401929/1‑139 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  1:494350/1‑140 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  4:58505/1‑140 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  2:89236/1‑140 (MQ=255)
                   ttttttAATGATTTTGCTCCAGCAATTACGATAGGGCATGGAAGATGTTCAGCTCCACCGCGACAACTGCCTGCCGATGAATCCATTGTCCATCAGCCAACGCAAAAGAAAATTAG‑CAAAATTAGCTTTCATAGTGAAAc                                                                                                                 >  8:627578/1‑140 (MQ=255)
                    tttttCATTATTTTGCTCCAGCAATTACGGTA‑GGCATGGATGATGTTCAACGACACGTCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  3:752010/140‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTCGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  5:85487/140‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  4:357934/140‑1 (MQ=255)
                       ttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACatat                                                                                                             <  1:608698/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGATCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCAGCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  5:405482/140‑1 (MQ=255)
                                             tACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CAAAATTAGGTTTCATAGTGCAACATATGCTTTAATGAATGTTCCATAtt                                                                                       >  4:40488/1‑140 (MQ=255)
                                              tcGGTTGGGCATGGATGATGTTCAACGACACGGCGACCACAGACTGCAGATGAATCCATTGTGCATCTGGCAAGGGAAAAGATAATTAG‑CCTAATTAGTTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                      <  7:432020/139‑1 (MQ=255)
                                                   aGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACt                                                                                 >  8:724640/1‑140 (MQ=255)
                                                     ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGAGTCATAGTGAAACATATGCTTTAATTAATGTTCCAGATTGAAACttt                                                                               >  5:66842/1‑140 (MQ=255)
                                                       cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAATAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                             >  4:805467/1‑140 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           >  3:784481/1‑140 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  5:529840/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  1:32221/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           >  6:145156/1‑140 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                          >  7:170313/1‑140 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCAACAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                          >  7:389833/1‑140 (MQ=255)
                                                           gatgatGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtg                                                                         >  3:674173/1‑140 (MQ=255)
                                                              gatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAt                                                                      <  7:176832/140‑1 (MQ=255)
                                                               atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  5:24649/1‑140 (MQ=255)
                                                               aatttCAACAACACGGAGACCACAAACGGCCGAAGAAACCAATTTTCAACAGGCAAGGGAAAAGAAAA‑TAACACAAATAAAGTTTCATAATTAAAAAAAAACATTAATTAATATTACAAATTTAAAAATTTACTTGTAtt                                                                     >  6:504857/4‑140 (MQ=255)
                                                                tgTTTAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  5:111881/1‑140 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGaa                                                                                    <  5:462640/124‑1 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  1:29108/1‑140 (MQ=255)
                                                                    cAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATAc                                                                >  3:264285/1‑140 (MQ=255)
                                                                     aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  2:250283/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  8:510842/1‑140 (MQ=255)
                                                                          cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  2:6347/1‑140 (MQ=255)
                                                                               cgcccACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                     >  7:328546/4‑140 (MQ=255)
                                                                                  ccACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGc                                                  <  5:487056/140‑1 (MQ=255)
                                                                                    actgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                             <  4:834471/95‑1 (MQ=255)
                                                                                    actgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                             >  3:834471/1‑95 (MQ=255)
                                                                                       gactgCCGATGCATCCTTTGTGCATCATGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                             <  8:767886/140‑1 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTTATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                             <  6:600412/140‑1 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                             <  4:50091/140‑1 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATAATCAAAATTGCGAGCCGGaa                                             >  2:761682/1‑140 (MQ=255)
                                                                                        actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTTATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAac                                            >  8:433661/1‑140 (MQ=255)
                                                                                           gCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                         >  4:611206/1‑140 (MQ=255)
                                                                                             cgtggAATCCTTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCATATTAGGTATCATAGGGACACATATGCTTTAATGAATGTGCCATATTGAAACTTTTACCTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  4:761770/136‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTGATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTTATACTTAAAATTGCGAGCCGGAACACCtt                                       <  5:750682/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  2:269548/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  7:719196/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  1:155098/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  7:786711/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  4:653054/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGCTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  7:267890/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATAAGGCAAGTGTACAGAAAATTAG‑CCAAATTAGTTTTCATAGTTAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAATTTGCGAGCCGGAACACCtt                                       <  2:100096/140‑1 (MQ=255)
                                                                                             cGATGAATACATTGTGCATCAGGCTTGTGATAAGAAAATTA‑CCCAACTTAGGTTTCATAGTGAAACATATGATTTAATGAATGTTACATATTGAAAGTTTTACGTGTATTAATCCTTAAAATTGCGAGCCGGAACACCtt                                       <  7:604167/140‑1 (MQ=255)
                                                                                                tGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAATTTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTg                                    >  7:631151/1‑140 (MQ=255)
                                                                                                      cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                              <  4:97167/140‑1 (MQ=255)
                                                                                                      cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGGGTATTAATACTTAAAATTGCGATCCGGAACACCTTTTGTCATaa                              >  8:627376/1‑140 (MQ=255)
                                                                                                         tgtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAggg                           >  1:874987/1‑140 (MQ=255)
                                                                                                          gtgCATCTGGCAAGGGAAAAGATAATTA‑TCCAATTTAGGGTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACTCGTTTTGTCATAAGGGa                          <  7:464002/140‑1 (MQ=255)
                                                                                                           tgCATCAGGCAAGGGAAAAG‑AAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  7:454072/140‑1 (MQ=255)
                                                                                                            gaaTCATGCAAGGTAAAAGAAAATTAG‑CCAAATTAGGTATCATAGTGAAACATAAGCTGTAATGAATGTTCCATCTTGACACTTTTACGTGTATTAATACTTAAAATTGCGAGCAGGAACACCTTTTGTCATAAGGGATg                        <  4:206959/138‑1 (MQ=255)
                                                                                                            gCATCAGTCAAGTTACAAGAAAATTAG‑CCACATTAGTTTTAATAGTGATACATATGCTTTAATGAATCTTCAATATTTAAACTTTTACGTTTATTAATACTTAAAATTGCGAGCCTTAACACCTTTTGTCATAAGGGATg                        <  8:214207/140‑1 (MQ=255)
                                                                                                            gCATCAGGCTAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:494812/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTCGGTTTCATAGTGAAACATATGCTTTACTGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:622852/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  8:214208/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  7:797564/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:447810/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:333013/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:641565/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:250283/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  6:432003/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGGTTCTTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  3:363733/140‑1 (MQ=255)
                                                                                                            ccATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  6:770089/139‑1 (MQ=255)
                                                                                                             cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGc                       >  5:754351/1‑140 (MQ=255)
                                                                                                             cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGc                       <  3:611206/140‑1 (MQ=255)
                                                                                                               tCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTGTCATAGTGAAACATATGCTTTAATGAATGTTCCATATAGAAACTTTTACGTGGATTTAAACTTAAAATTGCTAGCCGGTACACCTTTGGTCTTAAGGGATGCgg                     >  8:514425/1‑140 (MQ=255)
                                                                                                                 aGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGa                   >  2:306734/1‑140 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                  >  6:739778/1‑140 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTGTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTATTGTCATAAGGGATGCGGTat                  >  1:141043/1‑140 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  8:714272/140‑1 (MQ=255)
                                                                                                                         gaaaagaaaaTTAG‑CCAAATTAGGTATCATAGTGACACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGAGAGCAGGAAAACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  2:770210/140‑1 (MQ=255)
                                                                                                                          aaaagaaaaTTA‑TCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTgg          <  1:105250/140‑1 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  8:354789/124‑1 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        >  7:354789/1‑124 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg        <  2:365260/140‑1 (MQ=255)
                                                                                                                                  aTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATAGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTa  >  3:540961/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870777‑4871047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A