Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F2 I0 R1 145 5829.6 7950970 98.4% 7823754 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.94.8% 727.5 / 27.2 171intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (7/0);  new base . (76/86);  total (83/88)
Fisher's exact test for biased strand distribution p-value = 6.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 171 total aligned reads displayed.

CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTT  >  NZ_CP022686/4870777‑4871046
                                                                                                                                       |                                                                                                                                      
cTTGCACATCATTGATGAATTTTTTCATTATTTTGCTGCAGCAATTACGGTAGGGCATGGATGATGTTCACCGACACGGCGACCACTGACTGCCGATGAATCCATTGTCAATCAGGCAAGTGAAAAGATAATTAGCCaaa                                                                                                                                    >  7:710163/1‑140 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  8:463806/1‑140 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                    >  6:67054/1‑140 (MQ=255)
cTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACAACTGACTGCCGATGAATCAATTGTGCATCAGGAAAGGTAAAAGCAAATTATCCaaa                                                                                                                                    >  6:783475/1‑140 (MQ=255)
 ttGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGG‑AAAGAAAATTAG‑CCAAAtt                                                                                                                                 >  6:870983/1‑140 (MQ=255)
  tGCACATCATTGTTCAATTTTTTCATTATTTTGCTCCAGCAATTTCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATTAATCCATTGTGCATCATGCAAGTTAAAAGAAAATTAG‑CCAAAtt                                                                                                                                 <  8:252842/140‑1 (MQ=255)
  tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                 <  3:119755/140‑1 (MQ=255)
       atcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAgg                                                                                                                              >  1:585556/1‑138 (MQ=255)
        tcatTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTGCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                           <  7:2505/140‑1 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                          >  3:157523/1‑140 (MQ=255)
         catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑ACAAATTAGGTTTc                                                                                                                          >  4:902433/1‑140 (MQ=255)
          atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                         <  4:492611/140‑1 (MQ=255)
          atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                         <  7:41952/140‑1 (MQ=255)
          aatGATCTATTTTTTCATTATTTTGCGCGAGCAATTACGGTAGGGCATGGATTATTGTCAACTACACGGGGACATCTTACTGCCGATGACTCCATTTTGCATCAGGCATGGGATAAGTAAATTA‑TCCAATTTATGttt                                                                                                                           <  2:209475/136‑1 (MQ=255)
           ttGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGttt                                                                                                                           <  2:952424/137‑1 (MQ=255)
              ttCAATTTTTTATTTATTTTGCTAGAGCAATTACGGTATGGCATGGATGATGTTCAACGAAACGGCGACCAATGACTGCCGATGATTCCTATGTGCATCAGGGAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     <  4:969615/139‑1 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCTAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     >  5:153318/1‑140 (MQ=255)
              aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                     >  3:21689/1‑140 (MQ=255)
                   ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGCTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                >  4:766301/1‑140 (MQ=255)
                   ttttttCATAAATTTGCTCCAGCAATAACGGTAGTGCATGGAAGATGTTCAACGCCACTGCGACAACTCACAGCCGATGAATCCATAGTGCATCAGGCAAGGGAAAAGAAAAATAG‑ACAAATTAGGTTTCATAGTGAAAc                                                                                                                >  3:160843/1‑140 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                              <  3:163705/140‑1 (MQ=255)
                     ttttCATTATTTTGCTCCAGCAATTAAGGTAGGGGATGGAGGATGTTCATCGACACGGCGAGCACTGACTGCCGATGAATCCATTGTGCTTCAGGCAAGGGAAAAGAAAATTAG‑GCAAATTAGGTTTCATAGTGAAACat                                                                                                              <  6:315534/140‑1 (MQ=255)
                          attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCt                                                                                                         <  7:167139/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                  <  3:307818/140‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                    <  1:143870/138‑1 (MQ=255)
                                 tGCTCCAGCAATTACGGTAGGGCATGGATGATGTGCAACGCCACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAACAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                  <  4:199762/140‑1 (MQ=255)
                                              aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAAGTAG‑CCAATTTAGGTTTCATAGTGAAACATATGCTTTTATGAATGTTCCATATTg                                                                                     <  5:970388/140‑1 (MQ=255)
                                              aCGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                     <  7:548470/140‑1 (MQ=255)
                                               cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGa                                                                                    <  7:115186/140‑1 (MQ=255)
                                                ggTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGaa                                                                                   <  7:919047/140‑1 (MQ=255)
                                                  tAGGGCATGGATGAAGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATAAGATTTAATGAATGTTCCATATTGAAAc                                                                                 >  3:768195/1‑140 (MQ=255)
                                                      gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACtttt                                                                             <  4:230452/140‑1 (MQ=255)
                                                       cATGTATGATGTTAAACGACCCGGCGACCACTGACGGCCGATGAATCCATTGTGCATCAGGCAAGGGAATAGAAAATTAG‑ACAAATTAGGTTTCATAGTGAAACATATGCTTTAATGTATTTTCCATATTGAAACTTTTa                                                                            <  6:302079/140‑1 (MQ=255)
                                                       cATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            >  8:327212/1‑140 (MQ=255)
                                                         tGGATGCTGTTCAACGACCCGGCGACCACTGACTCCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  8:239772/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACTGCGACCACTGACTGCCGATGAATCCATATTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCTTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  7:620653/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            <  1:244057/138‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            >  1:605383/1‑138 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                            <  1:964643/138‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  7:33/140‑1 (MQ=255)
                                                         tGGATGATGTTCAACGACACGGCGACCACTGACGGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                          <  8:701010/140‑1 (MQ=255)
                                                          ggATGATGTTCACCGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTGAATGAATGTTCCATATTGAAACTTTTACgg                                                                         >  7:507385/1‑139 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACata                                                                                                             >  3:824374/1‑104 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACata                                                                                                             <  4:824374/104‑1 (MQ=255)
                                                          ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                         >  3:222775/1‑140 (MQ=255)
                                                            atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                         >  1:146095/1‑138 (MQ=255)
                                                               atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                    >  7:193933/1‑140 (MQ=255)
                                                                ttatCAACGACACGGCGACCACTGACTTACGATAAATCCATTGTGCATCAGGCAATGGAAAAGAAAAATAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                   >  8:545134/4‑140 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGCCTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                   >  7:608990/1‑140 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                   >  3:993386/1‑140 (MQ=255)
                                                                tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                   >  6:428047/1‑140 (MQ=255)
                                                                 gTTCACCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACATTTACGTGGATTaa                                                                  >  6:556202/1‑140 (MQ=255)
                                                                 gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATCCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                    >  1:731741/1‑138 (MQ=255)
                                                                  ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATTCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                   >  2:267764/1‑138 (MQ=255)
                                                                   tCAACGACACGGCGACCACTGCCTGCCGATGAATCCATTCTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATAAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTATTACGTGTATTaa                                                                  >  1:698923/1‑138 (MQ=255)
                                                                   tCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCGTCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                                  >  1:883669/1‑138 (MQ=255)
                                                                     aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATATTGAAAAATATGCTTAAATGAATGTTCAATATTGAACCTTTTCCGTGTATTAATACt                                                              >  6:214642/1‑140 (MQ=255)
                                                                            cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAtt                                                       >  8:778506/1‑140 (MQ=255)
                                                                              gCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAtt                                                       >  1:100088/1‑138 (MQ=255)
                                                                               cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCTAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                    <  5:729482/140‑1 (MQ=255)
                                                                                   cACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAg                                                  >  2:54648/1‑138 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAATGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                            <  5:289384/140‑1 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                            <  5:271334/140‑1 (MQ=255)
                                                                                       gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                            <  8:829721/140‑1 (MQ=255)
                                                                                          tgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAATCTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGCac                                           >  2:343558/1‑138 (MQ=255)
                                                                                            ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                       >  6:898610/1‑140 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACAcc                                        <  1:212222/138‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                      <  5:484219/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                      <  3:481364/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                      <  4:611242/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                      <  3:600766/140‑1 (MQ=255)
                                                                                             cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                      <  4:744841/140‑1 (MQ=255)
                                                                                             agATGAATCCATTGTGGATCAGGCAATGGAAAAGAAAATTAG‑CCAAATTAGTTTTCATAGTGATTCATAGGCTTTAATGAATGTTAAATATTTAAACTTTTTCGTGTATTAATACTTAAAATTGAGAGCATGAACACCtt                                      <  4:857999/139‑1 (MQ=255)
                                                                                              gATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCttt                                     <  5:330335/140‑1 (MQ=255)
                                                                                                 gAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGt                                  <  3:272629/140‑1 (MQ=255)
                                                                                                    tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTTAAACATATTCTTTAATGAATGTTACATATTGAAACTGTTACGGGTATTAATACTGACTATTGCGAGCCGGAACACCTTTTGTCAt                               >  4:538840/1‑140 (MQ=255)
                                                                                                     ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCTAGCCGGAACACCTTTTGTCATa                              <  8:113446/140‑1 (MQ=255)
                                                                                                     ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                              <  8:742852/140‑1 (MQ=255)
                                                                                                      cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                               >  2:609273/1‑138 (MQ=255)
                                                                                                        ttGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCAGGAACACCTTTTGTCATaa                             <  2:731741/138‑1 (MQ=255)
                                                                                                           tgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                      >  4:291746/1‑126 (MQ=255)
                                                                                                            gCATCAGTCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  1:797165/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  1:344007/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGa                         <  1:853208/138‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  8:162459/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  8:884841/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  7:874194/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  5:898610/140‑1 (MQ=255)
                                                                                                            gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  4:67414/140‑1 (MQ=255)
                                                                                                            gCAACAGGCAAGCGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGATACATAAGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                       <  4:584482/140‑1 (MQ=255)
                                                                                                                cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATATGGGATGCggg                   >  3:398963/1‑140 (MQ=255)
                                                                                                                cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                   >  3:899768/1‑140 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                   >  1:526179/1‑138 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat                 <  4:167585/140‑1 (MQ=255)
                                                                                                                  ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGACGGAACACCTTTTGTCATAAGGGATGCGGGat                 <  5:640840/140‑1 (MQ=255)
                                                                                                                        ggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGt           >  6:31232/1‑140 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGACCACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg       >  8:428260/1‑140 (MQ=255)
                                                                                                                            aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg       >  4:342004/1‑140 (MQ=255)
                                                                                                                             agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc      >  4:275380/1‑140 (MQ=255)
                                                                                                                               aaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc      >  1:468659/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTT  >  NZ_CP022686/4870777‑4871046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A