Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F35 I0 R1 271 8871.0 10354886 98.4% 10189207 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.97.3% 956.9 / 14.2 215intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (106/103);  total (108/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 216 total aligned reads displayed.

GTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCT  >  NZ_CP022686/4870775‑4871043
                                                                                                                                         |                                                                                                                                   
gTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTACAGCAATTACGGTAGGGCAGGGATGATGTTCATCGCCACGGCGTCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGcca                                                                                                                                   <  5:11822/140‑2 (MQ=255)
  cTTGCACATCATTGATCAATTTTATCATTATTTTGCTCCAGCAATTACGGTAGGGCTTGGATGATGTTCAACTACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCaaa                                                                                                                                 >  5:50855/1‑140 (MQ=255)
     gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                             >  8:908905/1‑140 (MQ=255)
     gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                             >  5:149745/1‑140 (MQ=255)
      cacaTCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAg                                                                                                                            >  8:817847/1‑140 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGTATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  2:1222106/1‑140 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAATTTAG‑ACAAATTAGGTTTc                                                                                                                       >  2:687775/1‑140 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  6:112125/1‑140 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  3:855356/1‑140 (MQ=255)
           catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATAGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                       >  2:985419/1‑140 (MQ=255)
                aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGCATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                  >  5:806900/1‑140 (MQ=255)
                aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                  <  7:834822/140‑1 (MQ=255)
                 tCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCAGGGATGATGTTCAACGACACGGCGACCCCTGACTGCCGATGAATCCATTGTGCATCCGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                 <  1:1095373/140‑1 (MQ=255)
                 tCAATTTATTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGCCTGCCGATGAATCCATTGAGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGGTTCATAGTg                                                                                                                 <  2:1109274/140‑1 (MQ=255)
                     ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGCCACGGCGACCACTGACTGCCGATGAATCCATTGTGCAGCAGGCAAGGGAAAAGAACATTAG‑ACAAATTAGGTTTCATAGTGAAAc                                                                                                             >  6:106744/1‑140 (MQ=255)
                     gtttttCATTATTTTGCTCCAGCAATTACGGAAGGGCATGGAGGAAGTTCATCGACACGGCGCCCCCTGACTGCCGCTGAATCCATTGAGCCTCAGGGATGCGAAAAGAAAATTAG‑CCAAATTAGTATTCAGACTGaacc                                                                                                             >  6:936726/2‑138 (MQ=255)
                       ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  8:278404/140‑1 (MQ=255)
                       ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                           <  4:584538/140‑1 (MQ=255)
                            attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCt                                                                                                      <  3:136444/140‑1 (MQ=255)
                                 tttGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACAGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaat                                                                                                 <  7:302467/140‑1 (MQ=255)
                                        cAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCttt                                                                                                    <  2:294264/130‑1 (MQ=255)
                                         cgCAATTACGGTAGGGCATGGATTATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAATGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                         <  6:508040/139‑1 (MQ=255)
                                          gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                        >  2:190883/1‑140 (MQ=255)
                                          gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                        >  4:874411/1‑140 (MQ=255)
                                          gCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTcc                                                                                        >  4:672371/1‑140 (MQ=255)
                                          gCAATTACGGTAGGGCATGGATGATGTTCAACCACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAACCATATGCTTTAATGAATGTTcc                                                                                        >  5:703011/1‑140 (MQ=255)
                                                   gTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGaaa                                                                               <  4:148796/140‑1 (MQ=255)
                                                       ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                           >  7:1182817/1‑140 (MQ=255)
                                                       ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                           >  6:565785/1‑140 (MQ=255)
                                                       ggCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACttt                                                                           <  2:1321054/140‑1 (MQ=255)
                                                          aTGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAAGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTAc                                                                        <  3:796698/140‑1 (MQ=255)
                                                          aTGGATAATCTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTAc                                                                        <  1:1272010/140‑1 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  8:346133/140‑1 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCATAATAGGTTTCATAGTGAAACAGATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  5:820163/140‑1 (MQ=255)
                                                           tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  3:1096327/140‑1 (MQ=255)
                                                            ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                              >  5:425558/1‑84 (MQ=255)
                                                                 atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                  <  6:678219/123‑1 (MQ=255)
                                                                 atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                  >  5:678219/1‑123 (MQ=255)
                                                                 atgGTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCGGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                 >  8:1006042/1‑140 (MQ=255)
                                                                  tgtaaaaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCATATAAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATAGAAACTTTTACGTGTATTa                                                                >  3:242644/6‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGTAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  6:803385/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  4:312804/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  5:947872/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  3:1197706/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  4:657277/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  8:447013/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                >  8:661519/1‑140 (MQ=255)
                                                                  tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATGAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAACCTTTTCCATGTATTa                                                                >  3:786376/1‑140 (MQ=255)
                                                                  tgTTCAACCACACGGCGACCACTGACTGCCGATGAATACATTCTGCAACAGGCAAGCGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTCAAACATATGCATTAATTAATGATCAATATTGAAACTATTACTTGTAttt                                                                >  3:1283297/1‑139 (MQ=255)
                                                                    ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                              <  6:706839/140‑1 (MQ=255)
                                                                       aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                           >  1:1116731/1‑140 (MQ=255)
                                                                       aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                           >  1:338671/1‑140 (MQ=255)
                                                                         cGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTa                                                         >  2:462135/1‑140 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGATACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                      >  8:1194295/1‑140 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       <  5:232881/123‑1 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                       >  6:232881/1‑123 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaaa                                                      >  6:262193/1‑140 (MQ=255)
                                                                            cacGGCGACCACTGACTGCCGATGAATCCATTGAGAATAAGTAAAGTGAAAATAAAATTAG‑ACAAATTAGCTTTCATAGAGAAACATATGCTTTAATGAATCTTCAATATTGAAACTTATACGTGTAATAATCCTTaaaa                                                      >  8:859688/1‑140 (MQ=255)
                                                                              cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAAtt                                                    >  3:1069551/1‑140 (MQ=255)
                                                                               ggCGACCACTGACTGCCGATGAATCCCTTGTGCATCAGGCTAGGGAAAAGATAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTACCATATTGAAACTTTTACGTGTATTAATACTTAAAATTg                                                   <  2:363996/140‑1 (MQ=255)
                                                                                 cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                 <  4:1069551/140‑1 (MQ=255)
                                                                                 cGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGGTCCATATTGAAACTTTTTCGTGTATTAATACTTAAAATTGCg                                                 >  5:1029952/1‑140 (MQ=255)
                                                                                 cGACCACTGACTGCCGATGAATCCATTCTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCg                                                 <  4:979585/140‑1 (MQ=255)
                                                                                     cACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGcc                                             >  6:441083/1‑140 (MQ=255)
                                                                                      actgactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCg                                            >  4:425144/1‑140 (MQ=255)
                                                                                         gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGaa                                         <  4:732254/140‑1 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  1:592760/140‑1 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  6:733514/140‑1 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  1:279463/140‑1 (MQ=255)
                                                                                               cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                   <  5:638027/140‑1 (MQ=255)
                                                                                                gATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGATTGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTCCGAGCCGGAACACCtta                                  <  8:529787/140‑2 (MQ=255)
                                                                                                gATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCttt                                  <  3:573535/140‑1 (MQ=255)
                                                                                                 aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtttt                                 >  7:388095/1‑140 (MQ=255)
                                                                                                      tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                            >  7:1000756/1‑140 (MQ=255)
                                                                                                      tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTACATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                            >  7:653755/1‑140 (MQ=255)
                                                                                                       ccATTGTGCATCAGGCAAGTGAAATGAAACTTA‑ACCAATTTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTCTATTAATACTTAAAATTGCGAGCCGGAACAGCTTTTGTCATa                           <  6:429830/140‑1 (MQ=255)
                                                                                                       ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAATTTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                           <  3:547014/140‑1 (MQ=255)
                                                                                                       ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                           <  2:812620/140‑1 (MQ=255)
                                                                                                         aTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAg                         <  2:868772/140‑1 (MQ=255)
                                                                                                            gtgCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACTCCTTTTGTCATAAGGGa                      <  7:450130/140‑1 (MQ=255)
                                                                                                              gCATCAGGCAATTGAAAACAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAGATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  6:378286/140‑1 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  3:672371/140‑1 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  2:1199339/140‑1 (MQ=255)
                                                                                                              gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  8:1022356/140‑1 (MQ=255)
                                                                                                              gCATAAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    <  8:507814/140‑1 (MQ=255)
                                                                                                               cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAACCTTTTACGTGTATTAATACTTAAAATTGCGCGCCGGAACCCCTTGTGTCATACGGGATGc                   >  2:518147/1‑140 (MQ=255)
                                                                                                               cATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGc                   >  5:1249375/1‑140 (MQ=255)
                                                                                                                    ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAATCTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTTTCATAAGGGATGCGGGat              >  2:1249421/1‑140 (MQ=255)
                                                                                                                    ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              >  6:360860/1‑140 (MQ=255)
                                                                                                                    ggCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGat              <  7:578853/140‑1 (MQ=255)
                                                                                                                         gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg         >  1:104365/1‑140 (MQ=255)
                                                                                                                         gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg         >  3:768819/1‑140 (MQ=255)
                                                                                                                         gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATAAGATTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGTAACACAGTTTGTCATAAGGGATGCGGGATATGAg         >  2:1042288/1‑140 (MQ=255)
                                                                                                                          ggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGt        >  6:150301/1‑140 (MQ=255)
                                                                                                                           gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg       <  4:129803/140‑1 (MQ=255)
                                                                                                                              aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGGTGCGGGATATGAGTGgcg    >  4:222485/1‑140 (MQ=255)
                                                                                                                              aagaaaaTTAG‑CCAAAGAAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTATACGTGTATTATTACTTAAAATTGCGAGCCGCAACACCTTTTGTCAGAAGTGATGCGGGATATGAGTGgcg    >  6:1152612/1‑140 (MQ=255)
                                                                                                                               agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcgc   >  3:896373/1‑140 (MQ=255)
                                                                                                                               agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCACATTGAAACTTTTACTTGTATTAATACTTAAAATTGCGAGCCGGTACACATTTTGTCATAAGGGATGCGGGATATGAGTGgcgc   >  1:896800/1‑140 (MQ=255)
                                                                                                                                    aTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                    >  1:1219439/1‑118 (MQ=255)
                                                                                                                                         |                                                                                                                                   
GTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCT  >  NZ_CP022686/4870775‑4871043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A