Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F2 I0 R1 130 6907.4 7549744 98.4% 7428948 136.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,870,9120C.97.4% 812.7 / 6.1 182intergenic (‑108/‑120)dhaK/dhaRdihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base . (94/83);  minor base T (1/2);  total (96/86)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 183 total aligned reads displayed.

CGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870774‑4871047
                                                                                                                                          |                                                                                                                                       
     tGCACATCATTGATCAATTTTTTCATTATTTTTCTACAGCAATTACGTTAGGGCATGCATGATGTTCAACGACACGGCGACCACTGACTTCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  6:917025/140‑1 (MQ=255)
     tGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAAtt                                                                                                                                  <  4:611613/140‑1 (MQ=255)
      gCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAAAGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTa                                                                                                                                 >  3:111768/1‑140 (MQ=255)
       cacaTCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAg                                                                                                                                >  3:197645/1‑140 (MQ=255)
            catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  3:223265/1‑140 (MQ=255)
            catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  1:84623/1‑140 (MQ=255)
            catTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTc                                                                                                                           >  7:367246/1‑140 (MQ=255)
             atTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          <  6:155177/140‑1 (MQ=255)
               tGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATa                                                                                                                        <  1:605945/140‑1 (MQ=255)
                 aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  1:196535/140‑1 (MQ=255)
                 aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGt                                                                                                                      <  1:752148/140‑1 (MQ=255)
                 aTCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTAAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTTCATCAGGCAAGGGAAAAGAAAAATAG‑CAAAATTAGGTTTCATAGt                                                                                                                      >  6:409838/1‑140 (MQ=255)
                  tCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                     <  3:513981/140‑1 (MQ=255)
                  tCAATTTTTTCATTATTTTGCTCCAGCAATTACGGGAGGGCATCGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                     <  6:175563/140‑1 (MQ=255)
                  gctagtttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGCCCACTGACTGCCGATGAATCCATTGTGCATCAGTCAAGGGAAATGAAAATTAG‑CCAAATTAGGTTTCATAGTg                                                                                                                     <  5:575429/135‑1 (MQ=255)
                      ttttttCATTCTTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCGATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 <  7:195436/140‑1 (MQ=255)
                      ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAAc                                                                                                                 >  3:690556/1‑140 (MQ=255)
                        ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  3:395033/140‑1 (MQ=255)
                        ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  7:54705/140‑1 (MQ=255)
                        ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  1:388981/140‑1 (MQ=255)
                        ttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTA‑CCCAAATTAGGTTTCATAGTGAAACat                                                                                                               <  8:6101/140‑1 (MQ=255)
                        ttatCATTATTTAGCTCCAGCAATTACGGTAGGGCATGTATGATGTTCAACGTCACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAATAGATACTTAG‑CCAAATTAGTTTTCATAGTGAAACat                                                                                                               <  2:513057/137‑1 (MQ=255)
                         tttCAATATTTGTCTCCATCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACATACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACata                                                                                                              <  4:812694/140‑1 (MQ=255)
                             attattTTGCTCCAGCAATTACGGTAGGGCATGGATGATGGTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCt                                                                                                          <  4:789078/140‑1 (MQ=255)
                                    tGCTCCAGCAATTACGGTAGGGCCTGCATTATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  2:59195/140‑1 (MQ=255)
                                    tGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCACCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTaatga                                                                                                   <  2:440996/140‑1 (MQ=255)
                                          agaaaTTACGGTAGTGCATGTATGATGTTCATCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTc                                                                                             <  6:810979/137‑1 (MQ=255)
                                                 aCGGTAGGGCAGGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATAGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTg                                                                                      <  2:105804/140‑1 (MQ=255)
                                                  cGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGa                                                                                     <  3:310848/140‑1 (MQ=255)
                                                    gTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGaaa                                                                                   >  1:37398/1‑140 (MQ=255)
                                                     tAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGCATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATCTTCCAGATTGAAAc                                                                                  >  6:353780/1‑140 (MQ=255)
                                                          cATGGATGATGTTCAACGACACGCCCACCACATACTGCCGATGAATCCATTGTGCATCACGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTATCAAACTGAAACATATGCTATAATGAATGATCCATATAGAAACTTTTa                                                                             >  3:103146/1‑140 (MQ=255)
                                                          cATGGATGATGTGCAACGACACGGCTCCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATATTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTa                                                                             <  3:864478/140‑1 (MQ=255)
                                                            tGGATGATGTTCATCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGAAATGGAAAAGAAAATTA‑TCCAAATTTGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  4:225838/140‑1 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  6:347640/140‑1 (MQ=255)
                                                            tGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACg                                                                           <  5:658939/140‑1 (MQ=255)
                                                             ggATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgt                                                                          >  5:559638/1‑140 (MQ=255)
                                                               atgatgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                        >  3:315018/1‑140 (MQ=255)
                                                               atgatgTTCAACGACACGGCGACCACTGACTCCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtgt                                                                        >  3:633979/1‑140 (MQ=255)
                                                                  atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  3:257246/1‑140 (MQ=255)
                                                                  atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt                                                                     >  4:256804/1‑140 (MQ=255)
                                                                  atgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCAATGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGATTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACTTGTAtt                                                                     >  7:495056/1‑140 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  8:835175/1‑140 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  4:960559/1‑140 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  7:856077/1‑140 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTA‑TCCAAATTAGGTTTCATAGTGAAACATATGCTATAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  6:663965/1‑140 (MQ=255)
                                                                   tgTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATAAGGCAAGGTAAAAGAAATTTAG‑CCAAATTAGGTTTCATAGTGCAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTa                                                                    >  7:555472/1‑140 (MQ=255)
                                                                    gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          >  1:720085/1‑85 (MQ=255)
                                                                    gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCa                                                                                                                          <  2:720085/85‑1 (MQ=255)
                                                                    gTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTaa                                                                   >  4:908258/1‑140 (MQ=255)
                                                                     ttCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAAt                                                                  <  4:115557/140‑1 (MQ=255)
                                                                       cAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGTAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATaa                                                                >  5:783800/1‑139 (MQ=255)
                                                                        aaCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACt                                                               >  3:15964/1‑140 (MQ=255)
                                                                         aCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCTTAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACtt                                                              >  8:220035/1‑140 (MQ=255)
                                                                         aCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACtt                                                              >  1:283668/1‑140 (MQ=255)
                                                                            acacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTaaa                                                           >  5:876149/1‑140 (MQ=255)
                                                                             cacGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAAAGAATGTTCCAGATTGAAACTTTTACGTGTATTAATACTTaaaa                                                          >  2:915801/1‑140 (MQ=255)
                                                                               cGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGATTGTTCCATATTGATACTTTTACGTGTATTAATAGTTAAAAtt                                                        >  4:245694/1‑140 (MQ=255)
                                                                                    aCCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCAATATTGAACCTTTTACGTGTATTAATACTTAAAATTGCGAg                                                   >  4:221554/1‑140 (MQ=255)
                                                                                     ccACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGTTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGc                                                  <  5:139004/140‑1 (MQ=255)
                                                                                          gactgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGTaa                                             <  2:55129/140‑1 (MQ=255)
                                                                                           actgCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAac                                            >  1:834459/1‑140 (MQ=255)
                                                                                               ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATAGGCTTTAATGAATGTTACATATTTAAACTATTAACTGAAGTAATACTTAAAATTGCGAGCCGCAACCCCt                                        >  3:620201/1‑140 (MQ=255)
                                                                                               ccGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGCTTTCATAGTGAAACATATGCTATAATGAATGTTACATATTGAAACTTTAACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                        >  7:485067/1‑140 (MQ=255)
                                                                                               ccGATGAATCCATAGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCATTAATGAATGTTCCATATTGAAACTATTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCt                                        >  3:737397/1‑140 (MQ=255)
                                                                                                cGATGAATCCCTTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGAGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  6:612263/140‑1 (MQ=255)
                                                                                                cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  1:522551/140‑1 (MQ=255)
                                                                                                cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  2:283668/140‑1 (MQ=255)
                                                                                                cGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCtt                                       <  2:623282/140‑1 (MQ=255)
                                                                                                     aaTCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTc                                  <  8:367246/140‑1 (MQ=255)
                                                                                                       tCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCAt                                >  6:437805/1‑140 (MQ=255)
                                                                                                       tCCAATGTGCATCAGGCAAGGGAAAAGAAAAATAG‑CCAAATTAGGGTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATAGAAACTTTTACGTGTATTAATACTAAAAATTGCGAGCCGGAACACCTTGTGTCAt                                >  8:746099/1‑140 (MQ=255)
                                                                                                        ccATTGTGCATCAGGCAAGTGAAAAGAAAATTAG‑CCAAATTAGGTTTCTTAGTGAAAAATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAATATTGCGAGCCGGAACACCTTTTGTCATa                               <  5:592503/140‑1 (MQ=255)
                                                                                                        ccATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTCTTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATa                               <  4:591299/140‑1 (MQ=255)
                                                                                                         cATTGTGCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATaa                              <  5:349039/140‑1 (MQ=255)
                                                                                                               gCATCATGCAATGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:77935/140‑1 (MQ=255)
                                                                                                               gCATCAGTCAAGGGAAAAGAAAATTAG‑ACAAATTAGGTTTCATAGTGAAACATATGATTTAATGAATGTTCCATATTGAAAATTTTACGTGTATTAATACTTAAAATTGCTAGCCGGAACACCTTTTGTCATAAGGGATg                        <  7:425745/140‑1 (MQ=255)
                                                                                                               gCATCAGGCTAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCTTATTGAAACTTTTACGTGTATTATTACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  3:776889/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTATGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:642259/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATTCTTTAATGAATGTTCCATATTGAAACTTTTAAGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  7:405038/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATACGGGATg                        <  4:822336/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  5:836173/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  1:335432/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:643578/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  8:421940/140‑1 (MQ=255)
                                                                                                               gCATCAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGATTTAATGAATGTTCCATATTGAAACTTTTAAGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATg                        <  2:421866/140‑1 (MQ=255)
                                                                                                                   cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                    >  1:79791/1‑140 (MQ=255)
                                                                                                                   cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                    >  6:111427/1‑140 (MQ=255)
                                                                                                                   cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                    >  5:453842/1‑140 (MQ=255)
                                                                                                                   cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCggg                    >  1:54716/1‑140 (MQ=255)
                                                                                                                   cAGGCAAGGGAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGCGATGCggg                    >  2:386168/1‑140 (MQ=255)
                                                                                                                          gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg             >  2:652651/1‑140 (MQ=255)
                                                                                                                          gggAAAAGAAAATTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAAAGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAg             >  8:293015/1‑140 (MQ=255)
                                                                                                                            gaaaagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTg           <  3:696958/140‑1 (MQ=255)
                                                                                                                               aagaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCAGATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg        >  6:643611/1‑140 (MQ=255)
                                                                                                                                agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCTGAACACGATATGTCTTAAGGGCTGCGGGATATGATTGgcgc       >  7:575194/1‑140 (MQ=255)
                                                                                                                                agaaaaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGACCACCTTTTGTCATAATGGATGCGCGATATGAGTGgcgc       >  4:523711/1‑140 (MQ=255)
                                                                                                                                    aaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtttt   <  2:7619/140‑1 (MQ=255)
                                                                                                                                    aaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtttt   >  4:48212/1‑140 (MQ=255)
                                                                                                                                    aaTTAG‑CCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCtttt   >  6:435446/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CGTCTTGCACATCATTGATCAATTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTAGGTTTCATAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTA  >  NZ_CP022686/4870774‑4871047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A