Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F13 I0 R1 125 11120.0 6942704 98.3% 6824678 135.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,041 A→C 25.1% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0410AC25.1% 30.9 / 4.4 60intergenic (+39/‑82)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (40/3);  new base C (2/11);  total (46/14)
Fisher's exact test for biased strand distribution p-value = 1.68e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCC  >  NZ_CP022686/4677913‑4678180
                                                                                                                                  |                                                                                                                                           
cTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑AATAATTACCGGGAccc                                                                                                                                    >  1:207034/1‑138 (MQ=255)
   ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGAcc                                                                                                                               >  4:627380/1‑139 (MQ=255)
   ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATAATGACCGGGCCCCGGCCg                                                                                                                               >  7:111711/1‑140 (MQ=255)
     cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCCGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CCTAAGGCCCGGGACCCGGCCg                                                                                                                               >  5:282903/1‑138 (MQ=25)
     cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑GATCATTACCGGGACCCGGCCg                                                                                                                               >  5:646899/1‑138 (MQ=255)
         cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CAACATGACCGGGACCCCGCCGCGGGaa                                                                                                                         >  7:402756/1‑140 (MQ=25)
         cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATAATGACCGGGACCCGGCCGGGGGaa                                                                                                                         >  7:601390/1‑140 (MQ=255)
         cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑AATCATGACCGGGACCCCGACggggg                                                                                                                           >  1:819146/1‑138 (MQ=255)
         cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CAAAATGACCCGGAACCGGACcggggta                                                                                                                         >  7:301110/1‑138 (MQ=25)
          aGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CCTAATTACCGGGACCCCGACgggggg                                                                                                                          >  2:357495/1‑137 (MQ=25)
           ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACgggggta                                                                                                                         >  1:87881/1‑136 (MQ=255)
                tttATGCCCACACCACCACCGGCCGCTGCGCTGATGTTTAAAACCGTCCGCCCACACTTATTGTCACCTTATTATAATTCTGCATTCCCTCTCGGT‑GGCCCCGGT‑CACCATGCCCGGGACCCGGACGGGGGAAAACGttt                                                                                                                  <  8:601390/139‑1 (MQ=255)
                 ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑GATCATGAGCCGGCCCCGGACGGGGGAAAACGttta                                                                                                                 >  8:580316/1‑139 (MQ=255)
                           aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTGCCCGG‑CCTGATGACCGGGTCCCGGACGGGGGGAAAAGTTTGAGTGGCACAt                                                                                                       >  3:691799/1‑140 (MQ=25)
                           aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑GATCATGACCGGGACCCGGACCGGGGAAAACGGTTCAGTGCcacaa                                                                                                       >  3:31698/1‑139 (MQ=255)
                           aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CATCATGACCGGGACCCGGACGGGGGTAAACGGTTACGTGCCACAt                                                                                                       >  8:248265/1‑140 (MQ=37)
                           aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CATAATGAACGGGCACCCGACGGGGGTAAAAGTTTAAGTGccccca                                                                                                       >  3:696036/1‑136 (MQ=25)
                             cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CCTCATGAACGGGACCCGGACGGGGGATAACGTTTCAGTGCCACAt                                                                                                       >  6:713743/1‑138 (MQ=255)
                               tCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CATCATGACCGGGACCCGGCCGGGGGAAAACGGTTGAGTGGCACATTCAc                                                                                                   >  3:245406/1‑140 (MQ=37)
                                cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGG‑CATCATGACCCGGACCCGGCCGGGGGGAAAACTTTAAGTGGCACCTTAAca                                                                                                  >  3:142942/1‑139 (MQ=25)
                                 aaCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATAAATACCGGGCCCCGGACGGGGGAAAACGTTTCAGGGGCACCTTCAc                                                                                                   >  5:781735/1‑138 (MQ=37)
                                     gcagcaGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CATCATTACCGGGACCCGGCCGGGGGCAAACGGTTAAGTGCCACATTCACGGACaa                                                                                             >  3:785970/1‑139 (MQ=37)
                                         ccgcgcTGATGTTTAAAACTGCCCGCCCACCCTCATTGTCCCCTTCTTATATTTCTGCATGGCCGCTCGGG‑GGCCCCGTC‑CCTCTTCCCCGGTACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTcac                                                                                         <  8:805811/138‑1 (MQ=255)
                                         cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CATGAATACCCGGACCCCGACGGGGGAAAACCTTTTAGTGCCACAATAACTGACaata                                                                                           >  2:82530/1‑135 (MQ=25)
                                           gcgcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGG‑CATGATGGCCGGGACCCGGTCGGGGGAAAACGTTTAAGTGCCCCATTACCTGCCATTcac                                                                                         >  5:545855/1‑138 (MQ=255)
                                             gcTGAAGGTTATAACCGTCAGGCCACACGCAATGTGACCTTATTATAATTCTGCATTGCCTCTCTGT‑GGGCCCGGT‑CATCATGACCGGTACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATa                                                                                     <  4:784094/140‑1 (MQ=255)
                                             gcTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CATCCATACCGGGCCCCCGCCGGGGGAAAACCATTCAATGCCACAATAACTGACAATCACaa                                                                                       >  6:587709/1‑138 (MQ=25)
                                              cTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CAACATGACCGGGACCCCGACGGGGGTAAACGTTTCATTGGCACCTTAACATACATTAACAATaa                                                                                    >  7:613666/1‑140 (MQ=255)
                                                      tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTACGTGCCACATTCACTGAACTTCACAATAACATTatat                                                                            >  7:474018/1‑140 (MQ=38)
                                                        taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑GATCATGACCGGGCCCCCGAAGGGGGCAAACGTTTCAGGGCCACATTCCCTGACATTCACAATAACATTatat                                                                            >  6:388251/1‑138 (MQ=255)
                                                             gTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CCTCATGGCCGGGACCCGGACGGGGGTAAACGTTTGAGTGCCACATTCACTGACATTCACAATAAAATGATATtaata                                                                       >  5:505228/1‑137 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGGCCCATTCACAGACATTCACAATAACATTATATTAATCACg                                                                    >  8:640058/1‑140 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGCT‑CGTAATTACCGGGACCCGGAAGCGTCAAAACGATTGATAGCCACAGATACACACAAAACAAATAACATGATATTCATCACg                                                                    >  4:342280/1‑140 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGG‑GCTCCTGACCGGGGCCCCGCCGGGGGGAAACGTTTGAGTGCCACAGTGACGGACATTCACAATAACAAGATATTAATCAcc                                                                    >  3:688635/1‑139 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGG‑GATCATGGCCGGGGCCCCGACGGGGGGAAACGTTTAAGTGGCCCATTAACTGACATTGACAATAAAATTATATTAATCAcc                                                                    >  3:246790/1‑139 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGG‑GATCAAGACCGGGCCCCGGCCGGGGGTAAACGGTTCAGTGCCCCAATCACTGACCGTAACAATAACAATATTTtcatgccg                                                                    >  7:640842/1‑136 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATTCCCGGGACCCGGACGGGGGGAAACGTTTGAGGGGCACAGTCACGGACATTAACAATAACATGATATTAATCACg                                                                    >  2:212086/1‑138 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTCCTGGCCACATTCACTGACATTCACAATAACATGATATTCATCACg                                                                    >  6:638857/1‑138 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CCTCATGACCGGGCCCCGGCCGGGGGTAAAAGTTTCAGTGGCACAATGACGGTAATTAACAATAACATGATTTTCATAACg                                                                    >  1:169921/1‑138 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGACACATGCGCGGCCATTCACCATCAAAGTATATTCATCACg                                                                    >  2:806612/1‑138 (MQ=255)
                                                                  cccACACTTAATTTTAGCTTATTATATTTCTGCATTCCATCTCTGT‑GGCCCGGGT‑CACCTTGCCGGGTACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGt                                                                <  4:22941/139‑1 (MQ=255)
                                                                    cacaCTTAATGTCCCTTTTTTATATTTCTGCATGGCCTCTGTGG‑GGGCCCGGC‑CATATTGCCGGGCCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                              <  8:121217/140‑1 (MQ=255)
                                                                    cacaCTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACGGGGGTAAACCTTTAAGTGCCACAATAACTGAAATTCACAAAAAAATGATATTAAACACGGAGt                                                                >  6:575084/1‑138 (MQ=255)
                                                                      cacTTATTGTGACTTTCTTAAAATTCTTCATGGCCCCTCTTT‑GTGCCGGGA‑ACTCTTCACGGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                            <  4:336528/140‑1 (MQ=255)
                                                                      caattaaTGTGCCCTTTTAATAATACTGCTTGCCCTCGCGGT‑GGTCCCGAC‑AATCACCCCCGGTACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                            <  4:178063/135‑1 (MQ=255)
                                                                      aacTGATTGTGACTTTCTAATAATTGTGATTGGCCTCGCGGG‑GGCCCCGGC‑AACCCTCCCGGGGCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                              <  2:20662/137‑1 (MQ=255)
                                                                                 aCCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCAAGACCGGGACCCGGCCGGGGGAAAACATTTAACGGCCACATTAACTGACAATCACAAAAACATGATATTAATAACGGAGTGACTATGTTACAGAt                                                 >  4:775050/1‑140 (MQ=255)
                                                                                 aCCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGG‑CATCCAGACCGGGGCCCCGCCGGGGGAAAAACGTTGAGTGCCCCATTCACTGACAATCACAATAACATGATATTCATAACGGAGTGACTAAGTTACAGAt                                                 >  8:295507/1‑140 (MQ=255)
                                                                                   cTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑GATAATGACCGGGACCCGGGCCGGGGGAAACGTTTAAGGGACACAATAACTTCCATTAAAAAAAAAATGATATTAATAACGGAGTGACTATGTTaaaaat                                                 >  1:73938/1‑133 (MQ=255)
                                                                                     tCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCCGT‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGAGATTCCTCACGTAGTGACTATGTTACAGATAGTc                                             >  4:67690/1‑140 (MQ=255)
                                                                                      attaaaaTTCTCCATTCCAGCTCGGG‑GGCCCCGGC‑CATCATGCCGGGGACCCGGAC‑GGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTc                                             <  1:862466/133‑1 (MQ=255)
                                                                                       tGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CAGAATTGACGGGGCCCCGACCGGGGTAAACGTTTAAGTGCCACCATAAAGGGCATTAAAAATAAAATTTTTTTAATAAAGGAGTTAATATGTTAAAGATatta                                             >  6:677942/1‑135 (MQ=255)
                                                                                            aTTCTTCTTTGCCTCTGTGT‑GCCCCCGGA‑CAAAATCCCGGGCCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgc                                        <  5:483464/138‑1 (MQ=255)
                                                                                                    ttcccTCTGTGG‑GGCCCCGGC‑CAACAGCCCGGGGCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTc                                <  2:242644/135‑1 (MQ=255)
                                                                                                      gCCGCTCTGG‑GGCCCCGGT‑CACCTGGCCCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctg                              <  2:87881/138‑1 (MQ=255)
                                                                                                        gcctctGTGGGGCCCGGTCCATCATCCCGGGTCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctg                              <  2:819146/136‑1 (MQ=255)
                                                                                                                  gTCCCGGT‑CATCATGACCGGGCCCCCGCCGGGGGAAAACGTTTAACTGCCACATTCACTGAACATAACAATAACAAGATATTAATCACGGAGTTACTATGTTACAGATAGTCGGCGCGCGGATTCTGCTGATCGCAGGAt                 >  4:523694/1‑140 (MQ=255)
                                                                                                                               atGACCGGTACCAGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc     <  4:617563/140‑1 (MQ=255)
                                                                                                                                tGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttctt      >  2:53387/1‑138 (MQ=255)
                                                                                                                                tGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttctt      >  2:278464/1‑138 (MQ=255)
                                                                                                                                  aCCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGcc  >  8:705683/1‑140 (MQ=255)
                                                                                                                                  |                                                                                                                                           
CTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGT‑GGTCCCGGT‑CATCATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGCC  >  NZ_CP022686/4677913‑4678180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A