Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F13 I0 R1 168 14435.1 8204878 98.4% 8073599 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,041 A→C 20.7% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0410AC20.7% 56.4 / 2.3 79intergenic (+39/‑82)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (53/6);  new base C (10/7);  total (66/13)
Fisher's exact test for biased strand distribution p-value = 6.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGC  >  NZ_CP022686/4677913‑4678179
                                                                                                                                |                                                                                                                                           
cTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATTACCGGGAccc                                                                                                                                    >  2:405316/1‑138 (MQ=255)
   ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGACACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATACTGCCCGGGACCCGGACg                                                                                                                               >  8:868663/1‑140 (MQ=25)
     cGTTCAGGACGGATATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATAGCCTCTCTGTGGTCCAGGGAAACATGACCGGGCCCCGGAcc                                                                                                                               >  1:665930/1‑137 (MQ=21)
       ttCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTGATAACAGTCAGGCCACACTGAATGTGCCCTGCTGCTAATTCTGCATTGCCTCGCTGTGGTCCCGGGCAGCATGACCGGGCCCCCGCCggggg                                                                                                                           >  6:491264/1‑140 (MQ=25)
          aGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATTACCGGGACCCGGGCcggggg                                                                                                                          >  2:272385/1‑137 (MQ=255)
           ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCAACATGACCGGGACCCGGGCgggggga                                                                                                                         >  3:816583/1‑136 (MQ=255)
           ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCATGACCGGGACCCGGACGGGGGaa                                                                                                                         >  2:796958/1‑138 (MQ=255)
           ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCAAGACCCGGACCCCGACGGGGGaa                                                                                                                         >  1:93755/1‑138 (MQ=255)
           ggCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGAATCAAGACCGGGACCCGGAAgggggta                                                                                                                         >  2:847378/1‑136 (MQ=25)
                gTTATCCCCACAGACTCAAGGGCCGCAGCGCTGAAGTTTATACCCGTCTGCCCACACTGATTGTGCCCTTATGATATTTCTCCTTTGCCGCTTTGTGGCCCGGGCCACCATGCCGGGGACCCGGACGGGGGAAA‑ACGt                                                                                                                   <  4:54104/138‑1 (MQ=255)
                      cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCATCTGATAATTCTGCATTGCCTATCTGTGGTCCCGGGCAGAAAGACCGGGGCCCCGACGTGGGAAA‑ACCTTTAAGTgg                                                                                                           >  7:751507/1‑139 (MQ=21)
                        cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGc                                                                                                           >  2:448249/1‑138 (MQ=255)
                        cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCATAAAGACCCGGGCCCGGGCGGGGGGAA‑ACGGTTCAGGGc                                                                                                           >  3:969515/1‑138 (MQ=25)
                           aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTGATCATTAACGGGACACGGACGGGGGAAA‑ACGTTTCAGTGCCCCAt                                                                                                      >  7:646388/1‑140 (MQ=37)
                           aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCcccaa                                                                                                      >  6:993292/1‑139 (MQ=255)
                             cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGACCCGGGCGGGGGAAA‑ACGTTTCAGTGCCACAt                                                                                                      >  2:238272/1‑138 (MQ=255)
                             cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCAAGACCGGGACCCGGCCGGGGGAAA‑ACGTTTCAGTGCcacaa                                                                                                      >  2:15856/1‑137 (MQ=255)
                             cATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCATTCCCCGGACCCGGACGGGGGAAA‑ACGTTTCAATGCcccaa                                                                                                      >  2:865288/1‑137 (MQ=37)
                               tccaCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCAAAATGCACGGGACCCCGACCTCGGAAA‑ACGGTTAGGGGCCCCAATCAc                                                                                                  >  8:661931/4‑140 (MQ=18)
                                 aaCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCCTTCCCGGGACCCGGGCGGGGGCAA‑ACGGTTCAGTGCCCCAATCAc                                                                                                  >  3:447737/1‑138 (MQ=25)
                                      ccgcagcGCTCAAGTTAAAAACATCCCGCCCACAGTTATGGTCACCTTATAAAAATTCTGCATGCCCCCTCGGGGGCCCCGGACATCATCCCGGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACAtt                                                                                           <  8:907180/138‑1 (MQ=255)
                                       agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGAGAATTCTGCATTGCCTCTCTGTGGTCCCCGGCCTCATGCCCGGGACCCGGGCGGGGGCAA‑CCGGTTCAGTGCCCCCTTCAcgtacag                                                                                            >  2:293682/1‑132 (MQ=25)
                                       agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATGATTCACCGGCCCCAGACGGGGGTAA‑ACGTTTCAGTGCCAAAATCACAGACaata                                                                                          >  5:619532/1‑137 (MQ=25)
                                       agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCATGACCGGGACCCCGACGGGGGAAA‑ACGTTTAAGTGCCACATTCACTGACAtta                                                                                          >  8:301144/1‑139 (MQ=255)
                                         cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATAATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACAATCACTGACAtta                                                                                          >  2:882233/1‑137 (MQ=255)
                                           gcgcTGAAGGTAATACAAGGCCGCCAACACTTATTGTGACATTTTTAAAATTATCAATTGCGTCTCTGTGGCCCGGTTCTTAATGACGGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTcac                                                                                        <  4:29567/138‑1 (MQ=255)
                                               tGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCAACAAGACCCGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTGACTGACATTCACAATa                                                                                    >  3:547439/1‑138 (MQ=255)
                                                      tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCTTCTCTGTGGTCCCGGTCATCATTACCGGGACCCGGGCGGGGGAAA‑ACGTTTAAGTGCCACCTTCACTGACATTAACAATAAAATGatat                                                                           >  7:461318/1‑140 (MQ=38)
                                                        taACAGTCAGGCCACACTGAATGTGAGCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATTAACGGGCCCCCGACGGGGGAAA‑ACGTTTCAGTGGCACAATCACAGACAATCACAATAACATGaaat                                                                           >  2:1038132/1‑138 (MQ=255)
                                                        taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCCGCCAGGCCGGGGCCCGGCCGGGGGCAA‑ACCTTTACGGGGCCCAAACACAGACAATGAAAATAACATGataata                                                                         >  6:42736/1‑137 (MQ=255)
                                                        taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGCCCCGGACGGGGGGAA‑ACGTTTCAGTGCCACATTCACTGACCTTCACAATAACATGATAtta                                                                         >  6:932089/1‑139 (MQ=255)
                                                              tCAGGCCCCACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGAATCATCTACCGGACCCGGAAGGGGGGAA‑ACTGTTAATTGCCACATAAACAGACATTAAAAATAAAAAGATAttaaaaaac                                                                   >  6:268107/1‑133 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCCTAATGACCCGGCCCCCGACGGGGGGAA‑ACGGTTACGTGGCACATTAACTGACATTCACAATAAAATGATTTtaataaac                                                                   >  7:473655/1‑136 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTAATCACg                                                                   >  5:776660/1‑140 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCATGACCGGGACCCGGACGGGGGTAA‑AAGTTTAAGTGCCACATTCACTGCCATTCACAATAACAAGATAATCATAAcc                                                                   >  5:947553/1‑139 (MQ=255)
                                                              tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCAGTCATCATGACCGGGACCCGGCCGGGGGAAA‑CCGTTTCAGTGCCACATTACCTGACATTCACAATAACATGCTATTCATCACg                                                                   >  8:61155/1‑140 (MQ=255)
                                                                tggCCACACTTAATGTTACCTTATTATAATCCTGCATTCCCTCTCTGTGGGCCCGGTCAACATCCCCGGGCCCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGa                                                                 <  6:776660/139‑1 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCCTCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTTCCACATTAACTGACATTAACAATAACATTATATTCATGACg                                                                   >  4:613853/1‑138 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCCCATTCACTGACATTCACAATAACATGATATTCATCACg                                                                   >  4:577794/1‑138 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGGCACATTCACAGACATTAACAAAAACATGATATTCATCACg                                                                   >  2:470287/1‑138 (MQ=255)
                                                                aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTACGTGCCACATTCACTGACATTCCCAATAACATGAAATTAATAACg                                                                   >  4:537780/1‑138 (MQ=255)
                                                                  gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAAAAACATGATATTCATAACggg                                                                 >  2:244625/1‑137 (MQ=255)
                                                                  gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCAGCATGACCGGGACCCGGCCGGGGGAAACACG‑TTAAGTGCCCCAGTCACGGACATTACCAAATACATGATATTCATAACCGAgg                                                               >  5:180829/1‑139 (MQ=25)
                                                                  gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCATTACAGGGCCCCCGACCGGGGGAA‑AAGTTTAATTGCCCCAATAACGGTCATTAACAATAACATGATATttataaagta                                                                 >  1:827192/1‑132 (MQ=255)
                                                                    cacaCTTATTGTGACATTCTGAAAATTCTGCATGCCCTATCTGTGGTCCCGGTCATAATGACGGGTACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                             <  8:566977/140‑1 (MQ=255)
                                                                    cacaCTGATTGTCACCTTCGGATATTGCTGCATGCCCCCTCTGTGGGCCCGGACACCATCACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGt                                                               <  4:943863/138‑1 (MQ=255)
                                                                    cacaCTGAATGTGACCTTCTGATAATTCTGCATTGCATCTCTGTGGTCCCGGTGATCATGACCGGGACCCGGACCGGGGAAA‑AAGTTTCAGGTCAACATTCACTGACAATCACAATTACATGATATTCATCACCGAGt                                                               >  4:409517/1‑138 (MQ=255)
                                                                      cacTGATTGTCACTTTCTTAAATTGCTGCATGGCCTCTGTGGGGCCCGGGTCAGCACCCCGGGGCCCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                             <  2:193489/138‑1 (MQ=255)
                                                                      aatttaaTGTCACATTTTGAAATTTCTGCTTTTCCTCTATGTGTTCCCGGTCATCATTACGGGTCCCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                             <  2:465627/133‑1 (MQ=255)
                                                                         tGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTGCTTATTACCGGGCCCCCGGCGGGGGTAA‑ACGGTTCAGTGCCACCTTAAACGCAATTCACAAAAACAAGATTTTAAACACGGAGTGACtt                                                          >  3:627783/1‑137 (MQ=255)
                                                                         tGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCCTAAAGACCGGGCCCCGGACGGGGGAAA‑ACGTGTCCGTGCCACATTGCAGGACAGTCACAAAAACAAGATATTCATCACGGAGTGACtt                                                          >  3:627781/1‑137 (MQ=255)
                                                                            aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGAACCGGACCGGGGAAA‑ACGTTTCACTTCCACATTCACTGACATTCACAATAACATGCTATTCATAAAGGATTGACTATGttt                                                     >  8:742423/1‑139 (MQ=255)
                                                                             tgtgACCTTCTGATAATTCTGCAATGCCTCTCTGTGGTCCCCCTAATCATGAACGGGACCCGGACCGGGGAAA‑ACGGTTAAGTGCCACAATCAATGTCAATCACAATAACAAGATAATGATCACCGACTGACTATGtt                                                      >  2:244182/1‑138 (MQ=255)
                                                                              gtgACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTAACTGACATTCACAATAACATGATATTAATCACGGAGTGACTATGTTa                                                     >  4:997836/1‑138 (MQ=255)
                                                                               tgACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCGGGGGAAA‑ACGTTTCAGTGGCACATTAACTGACATTCACAATAACATGATATTAATCACGGAGTGACTATGTTaa                                                    >  1:767795/1‑137 (MQ=255)
                                                                                  ccTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCGGGGGTAA‑AAGTTTCAGTGCCACCTTCACAGACATTACCAATTAAATGATATTAATACCGGAGGGACGATTTTACAgg                                                 >  2:416730/1‑137 (MQ=255)
                                                                                   cTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCATAATTACCGGGACCCCGACGGGGGGAA‑ACCTTTTATTGCCACAATAACTGTCAATAACAATAAAATGATATTAATAACGGAGTGACTATTTTACAGaa                                                >  2:347210/1‑137 (MQ=255)
                                                                                     tCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTGATAATGACCGGGGCCCGGAAGGGGGGAA‑AAGTTTAAGTGCCACATTAACTGACATTAACAATAAAATGATATTAAACAAGGAGTGGCTATGTGAAAGATaata                                            >  6:713138/1‑137 (MQ=255)
                                                                                     tCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTAACAATAACATGATATTCATAACGGAGTGAATATGTTACATATAGta                                            >  6:513854/1‑139 (MQ=255)
                                                                                       tGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCATGACCGGGACCCGGCCCGGGGGAA‑ACGTTTAAGTGCCCCATTGACTGACATTCACAATAACATGATATTCATAACGGAGTGACTATGTTAAAGATAGTc                                            >  4:375924/1‑138 (MQ=255)
                                                                                           aaTTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGATCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                        <  1:232859/138‑1 (MQ=255)
                                                                                           aaTTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAAGACCCGGACCCCGACGGGGGGAA‑ACCTTTAAGTGCCCCATTCACTGACATTCACAAAAACAAGATATTAATAACCGAGTGACTATGTTACAGATAGTCGgcg                                        >  2:383258/1‑138 (MQ=255)
                                                                                                tGCATTGCCTCTCTGTGGTCCCGGTCATCCTTACCGGGGACCGGGCGGGGGGCA‑ACGTTTAAGTGCCACATTAACTGACATTGACAATTACAAGATATTAATAACGGAGTGACTATTGTGAAAATATTCGGCGGGCGGAt                                 >  8:500394/1‑140 (MQ=255)
                                                                                                tGCATTGCCTCTCTGTGGTCCCGGGCATCATGGCCGGGACCCGGACGGGGGAAA‑ACGTTTAAGTGCCACATTAACTGCCATTGACAATTACATGATATTCAAAACGGAGTGACTATGTTAAAGATATTCGGCGCGCGGAt                                 >  8:618748/1‑140 (MQ=255)
                                                                                                tGCATTGCCTCTCTGTGGTCCCCGGGATAATGAACCGGGCCCGGGCGGGGGATA‑ACGGTTAAGTGGCCCCATCACTGGCATTAACCAAAACAATATTTTAATAACCGGCTGTCTATGTTAAAGATAGTCGGGGCGCAGAt                                 >  8:863499/1‑140 (MQ=255)
                                                                                                 gCATTGCCTCTCTGTGGTCCCGGTGATGAAGCCCGGGACCCGGCCGGGGGGAA‑ACCGTTGAGGGCCCCAGTGACGGCCATTCACCAAAACATTATTTTGATCACGGAGTGACTTTGTTTCCGCTTGTCGgcgcgcggctt                                >  8:596522/1‑135 (MQ=255)
                                                                                                  cATTGCCTCTCTGTGGTCCCCGTGATCATGACCGGGACCCGGACGGGGGGAA‑ACGTTTCAGGTCCACATTTACAGCCATTCACAATAAACTGATTTTCAAAACGGTGTGACTAAGTTAAAGTAAGTCGGCGCGCTGAt                                 >  4:128798/1‑138 (MQ=255)
                                                                                                    ttGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCGGGGGAAA‑ACGTTTCAGTGCCCCATTCACTAACATTAACAATAACAAGATATTAATAACGGAGTGACTATTTTAACTCTATTAGGCGCCCTGAtta                               >  3:204851/1‑137 (MQ=255)
                                                                                                         tctctGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGGAA‑ACCTTTCAGTGGCACAATCACAGACAATCAAAATAAAATGATATTAATAACGGAGTTACTATGTTACAGATAGTCGGCGCGCTGATTatgctg                          >  2:488083/1‑138 (MQ=255)
                                                                                                           tctGTGGCCCCGGTCAACATCCCGGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGAt                        <  3:568195/138‑1 (MQ=255)
                                                                                                           gctGGGGCCCCGGTCATCATGCCGGGCACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGAt                        <  4:483917/137‑1 (MQ=255)
                                                                                                            ctGTGGTCCCGGGCATCATGACCGGGACCCGGGCGGGGGAAA‑AAGGTTCAGGGCCACATTGACTGACATTCACAATAACATGATATTCATAACGGAGTGACTATTTTAAAGATAGTAGGCGCGCTGATTCTGCTGATCGc                     >  8:122348/1‑140 (MQ=255)
                                                                                                              gtgGTCCCCGTCATCATGACCGGGACCCGGTCGGGGGGAA‑ACGTTTCAGTGCCCCATTGACTGACATTCACAATAACATGATATTCATCACGGTGGGACTTTGTTACAGATAGTCGGGGCGCTGATTCTGCTGTTCGc                     >  2:838075/1‑138 (MQ=255)
                                                                                                                      ggTCATCATCCCCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTg             <  2:767795/138‑1 (MQ=255)
                                                                                                                            catGACCGGGACCCGGCCGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttctt     >  6:248750/1‑140 (MQ=255)
                                                                                                                            catGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATAGCAGGATTTGCCAttctt     >  6:865649/1‑140 (MQ=255)
                                                                                                                             atGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct      >  4:934813/1‑138 (MQ=255)
                                                                                                                              tGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGTAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttctt     >  2:787689/1‑138 (MQ=255)
                                                                                                                               gACGGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGc  <  6:848371/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                           
CTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACGGGGGAAA‑ACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCGC  >  NZ_CP022686/4677913‑4678179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A