Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F2 I0 R1	130	5456.6	6845820	98.4%	6736286	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP022686	4,678,041	A→C	27.1%	intergenic (+39/‑82)	agn43 → / → CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP022686	4,678,041	0	A	C	27.1%	25.4 / 7.7	63	intergenic (+39/‑82)	agn43/CIW80_RS23450	autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (41/2); new base C (7/8); total (53/10)
Fisher's exact test for biased strand distribution p-value = 1.16e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTC  >  NZ_CP022686/4677916‑4678177

ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCTTTG‑CCTCTCTGTGGTCCCGGGAATCATGACCGGGA‑CCCggccc                                                                                                                            >  8:475161/1‑137 (MQ=255)

ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCCGGA‑CCCGGGCg                                                                                                                            >  8:239447/1‑140 (MQ=255)

ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGGCTCATTACCGGGG‑CCCCGACg                                                                                                                            >  1:41689/1‑140 (MQ=21)

ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCAAAAAGACCGGGG‑ccccgcac                                                                                                                            >  1:72607/1‑135 (MQ=21)

    ttCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTGCCCGGGATCATGGACCGGC‑CCCCGACggggg                                                                                                                        >  7:719530/1‑140 (MQ=21)

      cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATGAACGGGC‑CCCGGACCGGGGaa                                                                                                                      >  1:481000/1‑140 (MQ=255)

      aaggaaggTTATGCCAACACCATCAACGGCAGCAGAGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTTCTTTG‑ACTCTCTGTGGTCCAGTTAAACAAAACCGGGA‑CCCCGCCGCGGGaa                                                                                                                      >  6:543279/7‑140 (MQ=21)

         gCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCAGTCATCAAGACCGGGC‑CCCGTACGGGGGAAAAc                                                                                                                   >  8:394174/1‑140 (MQ=25)

               tATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTGTGATAATTCTGCAGTG‑CCTCTCTGTGGTCCCGGACATAATGCCCGGGA‑CACGGGAGGGGGAAAACGTTTCa                                                                                                             >  2:96735/1‑140 (MQ=25)

                        aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCCCAt                                                                                                    >  6:678205/1‑140 (MQ=255)

                        aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGCGGATCAAGACCGGGC‑ACCGGAAGCGGGAAAAACGTTCAGTGCcacaa                                                                                                    >  1:415457/1‑139 (MQ=25)

                        aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCAGGTCATCATTACCGGGT‑CCCGGTCGGGGGAAAACGTTTCAGTGCCACAt                                                                                                    >  7:677755/1‑140 (MQ=255)

                        aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGAAAATTCTGCATTG‑CCTCTATGTGGTACCGGGAATAATGACCGGGACCCCGGAC‑GCGGTAAACGGTTAAGTGcccccg                                                                                                    >  8:101255/1‑136 (MQ=21)

                             cAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATTGCCGGGA‑CCCGGACGGGGGAAAAAGTTTAAGTGCCACATTCAca                                                                                               >  8:415642/1‑139 (MQ=37)

                                  gcagcaGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCCTCATGACCGGGA‑CCCGGCCGGGGGAAAACGGTTAAGTGCCACATTCACTGACAt                                                                                          >  2:78326/1‑140 (MQ=255)

                                   cagcacCGCTTATGTTTAAAACAGCCAGCCAACACTTATTGTTACATTCTCATAATAATGCATTC‑CCTCTGTGGGCTCCCGTAAAAAATCACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAtt                                                                                         <  8:656918/140‑1 (MQ=255)

                                    agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGCCCGGGA‑CCCCGCCGGGGGAAAACGTTTCAGTGCCACATTCACTGACAtta                                                                                        >  8:770894/1‑139 (MQ=255)

                                      cagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTAAAAATTACCCGGACCCCGCCCGGGGGAAAAC‑TTTAACTGGCACATTGAATGAAAttgaa                                                                                      >  7:230171/1‑137 (MQ=16)

                                       agcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCAAGACCGGGG‑CCCCGGCGGGGGAAAACGTTTAAGTGCCACAATAACTGACATTaaca                                                                                     >  7:153814/1‑140 (MQ=37)

                                        gcgcTTATGTTTATAACAGACCGCCCACACTTATTGTCACCTTCTTAAAATTCTCAATGC‑CCTCTCTGTGGCCCCGCCCAACATCCCGGGTA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACaa                                                                                    <  6:828741/140‑1 (MQ=255)

                                           cTGAAGGTTATAACTGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCCTGCCCGGGT‑CCCGGACGGGGGTATCCCTTTGAGGTCCCCAATAACTCACCTTCACAaaaa                                                                                 >  3:353265/1‑140 (MQ=255)

                                              aaGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGGATGATGACCGGGT‑CCCGGGCGGGGGGTAACGGTTCAGTGGCACCTTAACCGCCCTTCACAATAACAt                                                                              >  1:161983/1‑140 (MQ=255)

                                              aaGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCTAAATGCCCGGGA‑CCCGGGCGGGGGTAACCGTGTCTGTGACACATTAACTGCCAGTCAAAATaaaaa                                                                              >  3:587683/1‑137 (MQ=255)

                                                   tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTGCCCGTAATCATGACAGGGG‑CGCGGGACGGGGAAAAAGTTTCATTGGACCAATAAAAGACATTCACAAAAAAAAGatat                                                                         >  7:582254/1‑140 (MQ=255)

                                                   tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATGATGAACGGGA‑CCCGGGCGGGGGGAAAAGGTTCAGTGGCACATTCACAGCCATTCACAATAACATGatat                                                                         >  2:258171/1‑140 (MQ=37)

                                                   tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGC‑CCCGGCCGGGGGGAAACGTTTCAGTGCCCCATTCACGGACAATCACAATAAAATGatat                                                                         >  5:778292/1‑140 (MQ=38)

                                                   tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATGGCCGGGA‑CCCGGACGGGGGGCAACGTGTCCGGGGCACCTTCGCGGACATTCACCATAACATGgtat                                                                         >  2:72542/1‑140 (MQ=25)

                                                    ataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCATGACCGGGC‑CCCGGGCGGGGGAAAACGTTTCAGTGGCCCATTCACTGACCTTAACAATAACATGATAtt                                                                        >  1:518587/1‑140 (MQ=255)

                                                     taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATGACCGGGA‑CCCGGACGGGGGGAAACGTTTCAGTGGCACATTAAAATACAATAACAAAAAAATGATAtta                                                                       >  1:211961/1‑139 (MQ=255)

                                                     aaaCAGTCCGCCCACACTTATTGTGACCTTCTGATAATTCTGAATTG‑CCTCTTTGGGGCCCCGGCCATCATCACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATtc                                                                       <  4:748869/139‑1 (MQ=255)

                                                        cAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGGCACATTGACTGACATTAACAATAACAAGATATtaata                                                                    >  1:407095/1‑139 (MQ=255)

                                                           tCAGGCCCCACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCGCTGTGGTCCCCGTCAAAATTACCGGGC‑CCCGGGCGGGGGGCAACGTTGGCGTGCCACCTTCCCTGCCATTCTCAATTACAAGCTATTCAGCAcc                                                                 >  2:197457/1‑139 (MQ=255)

                                                           tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGA‑ACCGGGCGGGGGAAAACGTTTAAGTGCCACATTCACTGACATTAACAAAAAAAAGATATtcatcaac                                                                 >  6:277165/1‑138 (MQ=255)

                                                           tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATAATGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTAACTGACATTCACAATAACATGATATTAATCTCg                                                                 >  2:70988/1‑140 (MQ=255)

                                                           tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCATGCCCGGGA‑CCCGGACGGGGGTAAACCTTTCAGTGCCACAATAACTGACATTCACAATAACAAGATATTAATAACg                                                                 >  4:854452/1‑140 (MQ=255)

                                                           tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGCATCATGCCCGGGC‑CCCGGCCGGGGGGAAACCTTTAAGTGCCCCATTCACTGACATTCCAAAAAACCTGATATTCATCAcc                                                                 >  3:391402/1‑139 (MQ=255)

                                                           tCAGGCCACACTGAATGTGACCTTCTGATAAGTCTGCATTG‑CCTCTCTGTGGTCCCGGTGCTCCTGACCGGGA‑CCCGGCCGGGGGGAAACGTTTAAGTGCCACATTAACGGCCATTCACAATAATATGATATTCATAACg                                                                 >  6:508635/1‑140 (MQ=255)

                                                           cccgcccACAATGATTGTTACATTTTTATAATTCTGAATGC‑CCTCTCTGTGGGCCCGGTAATAATCCGGGGCA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACg                                                                 <  3:633241/135‑1 (MQ=255)

                                                             aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGA‑CCCGGGCGGGGGGAAACGTTTAAGTGCCACATTAAAAGACATTCACAATAACAAGATATTCATAACGGa                                                               >  4:805017/1‑140 (MQ=255)

                                                               gCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCGTCATGACCGGGT‑CCCGGCCCGGGGGAAACGTTTGAGTGCCACAGTAACAGACAATAACAATAACATGATATTTATAACGGAGt                                                             >  7:498484/1‑140 (MQ=255)

                                                               gCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATTACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTAACTGACATTAACAATAACATGATATTCATCACGGAGt                                                             >  8:664781/1‑140 (MQ=255)

                                                                   aacTGAATGTGACCTTTTTAAATTTTTGCATTG‑CCCCTCGGGGGCCCGGGCCAACAGTCCGGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                         <  4:234672/139‑1 (MQ=255)

                                                                       tattgtaACCTTATTATAATTCTTCATTG‑AGTGTTGGGGCACCCGGTCACCCTCCCTGGCC‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACGGAAATTCACAATAACATGATATTCATCACGGAGTGACTATGt                                                     <  8:578455/137‑1 (MQ=255)

                                                                       tattgggACCTTCTTATATTTCTGCATTG‑CCGCTCTTGGGCCCCGTCCATCATCCCCGGCA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGt                                                     <  4:109848/134‑1 (MQ=255)

                                                                         aTGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGGCATCAAGACCGGGA‑CCCGGGCGGGGGTAAACGTTTGAGTTCCACATTCACTGGCATTAAAAATAACAAGATATTCATACCCGAGGGACTTTGttt                                                   >  3:826780/1‑139 (MQ=255)

                                                                          tgtgACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATGACCGGGT‑CCCCGCCGGGGGAAAACCTTTCAGTGCCACAATCACAGACATTCACAATAACATGATATTCATCAAGGAGTTACTATGTTaa                                                  >  2:555349/1‑139 (MQ=255)

                                                                              aCCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATGACCCGGG‑CCCGGCCGGGGGAAAACGTTTCAGTGCCACATTAACTGAACATCACAATAACATGATATTACTAACGGAGTGACTATGTTTCAGAt                                              >  5:508007/1‑140 (MQ=255)

                                                                              aCCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCAAGACCGGGC‑CCCGGACGGGGGAAAACGTTTCAGTGCCACAATCACAGGCATTCACAAAAAAATTATATTCAAAAAGGAGTGTCTATGTTACAGAt                                              >  1:403841/1‑140 (MQ=255)

                                                                              aCCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGGAATCATGGCCGGGA‑CCCCGAACGGGGGAAACGTTTCATTGCCACATTCACAGACATTCAAAATAAAATAATATTAAAAACGTAGTTACTATTTTaaaaat                                              >  4:84064/1‑135 (MQ=255)

                                                                                cTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGG‑CCCGGCCCGGGGAAACCCTTTAAGTTCCACATTCACTGACAATCACCATTACATGATATTAATCACGGAGTGACTAGTTTACAGAAAg                                            >  4:455458/1‑140 (MQ=255)

                                                                                cTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCAAGACCCGGA‑CCCCGACGGGGGGAAACGTTTAAGTGCCCCAATCAAGGACAATAACAATAACATGATATTCATCACGGAGAGACTATGTTACAGATat                                            >  4:844294/1‑139 (MQ=255)

                                                                                 ttgtaatatTTCTG‑ATTGCCCTCTTTGTGGCCCCGGAAACCA‑GCCCGGGCCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGt                                           <  5:474766/135‑1 (MQ=255)

                                                                                  tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTGCTAATGGCCGGGC‑CCCGGCCGGGGGGAAACGTTTCAGTGCCACATTCACTGACATTCACCATAACATGATATTAATCACGGAGTGACTATGTTACAGATAGta                                          >  6:839808/1‑139 (MQ=255)

                                                                                  tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGCCCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCAAAATAACATGATATTCATAACGGAGTGACTATGTTACAGATATTc                                          >  6:339192/1‑140 (MQ=255)

                                                                                  tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTGATAATGAACGGGC‑CCCGGGCGGGGGCAAACGTTTCAGTGCCACATTAACTGACATTAACAATAACATGATATTAATAACCGAGTGGATATTTTTAAGATAGta                                          >  7:90104/1‑139 (MQ=255)

                                                                                  tCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCCGTCATCATGACCGGGA‑CCCCGACGGGGGAAAACGTTTGAGTGCCACATTAACGTACATTCACAATAACCTGATATTAATAACGGAGTGTCTATGTTAAAGCTAGtg                                          >  6:565328/1‑139 (MQ=255)

                                                                                             tGCATTG‑CCTCTCTGTGGTCCCGGTAATCATGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCCCATTCACTGACATTCACAAAAACAAGATATTCATCACGGCGTGACTATTTTACAGCTAGTCGGCGCGCGGAt                               >  7:475835/1‑140 (MQ=255)

                                                                                                          gtggggccccGGGAATAATGCCGGGGC‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGc                   <  4:580491/133‑1 (MQ=255)

                                                                                                          ctGTGGTCCCGGTCATCATGACCGGGA‑CCCGGCCGGGGGGAAACGTTTAAGTTCCACATTAACGGACATTAACAATAACAAGATATTAAACACGGAGTGACTATGTTAAAGATATTCGGCGCGCTGATTCTGATGATCGc                   >  4:593261/1‑140 (MQ=255)

                                                                                                                  cccgggatcatGACCCGGA‑CCCCGCCGGGGGGAAACGGTTAAGGGCCCCATTAACTGACATTCACAATAACATTATATTACTAACGGAGTGTCTAATTTTAAGATATTCGGCGCGCTGATTATGGTGATAGCCGGAtttt           >  6:20831/7‑139 (MQ=255)

                                                                                                                   gcgcaatcatcCCCGGCC‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGc          <  3:805017/135‑1 (MQ=255)

                                                                                                                          catGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACGTTGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTTCCAttctt   >  8:725627/1‑140 (MQ=255)

                                                                                                                          catGACCCGGA‑CCCGGCCCGGGGAAAACGTTTCAGTGCCACATTCACTGACCATCACAATAACAACATAATCATCACGCAGTGACTATCTTCCACAAATTCGGAGCGCTCCTTCTGCTGAACGAATGATTTGCCAttcat   >  4:154035/1‑138 (MQ=255)

                                                                                                                           ttcaCCGGCC‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc  <  1:385150/137‑1 (MQ=255)

GGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTG‑CCTCTCTGTGGTCCCGGTCATCATGACCGGGA‑CCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTC  >  NZ_CP022686/4677916‑4678177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A