Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F36 I0 R1 229 12610.8 9774876 98.4% 9618477 135.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,041 A→C 19.3% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0410AC19.3% 49.0 / 3.4 81intergenic (+39/‑82)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (57/5);  new base C (8/8);  total (68/13)
Fisher's exact test for biased strand distribution p-value = 4.08e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCG  >  NZ_CP022686/4677904‑4678178
                                                                                                                                             |                                                                                                                                          
aaTATCACCGTGGGCGTTCAGGCGGGTTATGCCCACCGCATCAAGGGCAGCCGCGCTGAAGTTTATAACA‑GTCAGGCCACCCTTAATGTCACCTTCTTATAATTATGCATTGCCTCTCTGTGGC‑CCCTGTC‑AT‑CATCAcg                                                                                                                                          <  6:683085/140‑2 (MQ=255)
aaTATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATTAcc                                                                                                                                          >  5:615819/1‑140 (MQ=255)
  tatCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGCCCgg                                                                                                                                        >  3:463186/1‑140 (MQ=255)
       cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCGGGG‑AA‑GATGACCCGGAccc                                                                                                                                   >  7:27351/1‑140 (MQ=21)
         cTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑CT‑CATGGCCGGGAccc                                                                                                                                   >  1:647498/1‑138 (MQ=255)
              cGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGACg                                                                                                                              >  2:707305/1‑138 (MQ=255)
               gTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑AATGACCGGGACCCGGACgggg                                                                                                                           >  8:129074/1‑140 (MQ=255)
                 tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGCCgggggg                                                                                                                         >  3:293865/1‑139 (MQ=255)
                 tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATACTGCATTGCCTCTCTGTGGT‑CCCGGTG‑AT‑CATGAACGGGAACCGGACGGGGGa                                                                                                                         >  4:501326/1‑140 (MQ=255)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCCGGG‑AA‑GATTACCGGGCCCCGGGCcggggga                                                                                                                        >  3:832463/1‑138 (MQ=18)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTG‑AT‑CATGACCGGGCCCCGGACgggggga                                                                                                                        >  6:833401/1‑138 (MQ=255)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑AATTCCCGGGACCCGGACGGGGGaa                                                                                                                        >  4:147574/1‑140 (MQ=255)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑AAATACCGGGCCCCGGCCGGGGGaa                                                                                                                        >  7:1013549/1‑140 (MQ=21)
                         tttATGCCCACCTCACCACGGCCTGCAGCGCTTATGGTTATAACC‑GCCCGCCCACACTTACTGGCCCCTTCTTATATTTCTGCTTGCCCTCGTTGTGGG‑CCGGGTC‑AG‑CAGCCCCGGGACCCGGACGGGGGAAAACGttt                                                                                                                 <  8:488768/139‑1 (MQ=255)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGGC‑TC‑CAGACCCGGGCCCCGCCCGGGGGaaaccttta                                                                                                                 >  8:246077/1‑134 (MQ=18)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑AT‑CATGACCGGGACCCGGGCGGGGGAAAAAGtttg                                                                                                                >  7:112006/1‑139 (MQ=255)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTATGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑TT‑CATTACCCGGACCCGGACGGGGGGAAACGttta                                                                                                                >  8:599594/1‑139 (MQ=37)
                               cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGACGGGGGCAAACGTTTCAGTGc                                                                                                           >  7:965243/1‑140 (MQ=255)
                               caccccacATCACCGGCAGCACCGCTTATGTTAAAAACT‑GTCAGCCCACAGTAACTGTTACCTTCTTAAAATTCTGCCTGCCCCCTGTGTGGC‑CCCGGCA‑TT‑CACCCCCGGGACCCGGACGGGGGAAAACGTTTCAGTGc                                                                                                           <  8:841195/133‑1 (MQ=255)
                                 cacaGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGCTC‑AT‑AATGACCGGGACCCGGACGGGGGAAAAAGTTTCAGTTCca                                                                                                         >  5:492751/1‑140 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCGGGG‑ATAGATTA‑CGGGGCCCGGAGGGGGGGAAACGGTTGAGTggccccg                                                                                                      >  3:399049/1‑134 (MQ=18)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGAACGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACAt                                                                                                      >  8:522005/1‑140 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑AATGACCGGGCCCCGGGCCGGGGTCAACGGTTGAGTGGCACAt                                                                                                      >  3:495068/1‑140 (MQ=25)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑CATGACCGGGTCCCCGACGGGGGAAAAAGTTTCATGGCcacaa                                                                                                      >  6:1014853/1‑139 (MQ=37)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑CATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTggcccaa                                                                                                      >  4:790791/1‑134 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGA‑CCCGGTC‑AA‑ACAGAACGGGCCCCGGACCCGCGAAAACGTATCAGTGCcccaa                                                                                                      >  8:694042/1‑139 (MQ=21)
                                      cATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGTCGGGGGAAAACGTTTCAGTGCCACATTc                                                                                                    >  6:464850/1‑140 (MQ=255)
                                      cATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AA‑CAAGAACCGGGCCCGGACGGGGGTAAACCTTTCAGGTCCACAtta                                                                                                    >  7:979153/1‑139 (MQ=25)
                                        tCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CAGGACCGGGACCCGGCCGGGGGAAAACGTTTCAGTGCCACATTCAc                                                                                                  >  3:868192/1‑140 (MQ=255)
                                          aaCGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCCCACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTG‑CT‑CATGCCCCGGGCCCCGACGGGGGCAAACGGTTCTGTTCcccaataac                                                                                                  >  1:666909/1‑133 (MQ=21)
                                          aaCGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑AT‑CATGACCGGGACCCGGGCGGGGGAAAACGTTTCAGTGCCACCTTCAc                                                                                                  >  2:130308/1‑138 (MQ=255)
                                           aCGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AA‑CAAGACCGGGACCCGGACGGGGGAAAACGGTTCAGTGCCCCATTAACTGa                                                                                               >  4:807449/1‑140 (MQ=37)
                                           aCGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAACTCTGCATTGCCTCTCTGTGGTGCCCGGGC‑‑T‑CATGACCGGGCCCCGGCCGGGGGGAAACCGTTGAGTGCcccaatacca                                                                                                 >  1:383159/1‑132 (MQ=21)
                                             ggCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AA‑CATGAACGGGACCCGGGCGGGGGCAAACAGTTCAGTGGCCCAATAACTGa                                                                                               >  1:600161/1‑138 (MQ=21)
                                                agcagcGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑AT‑CATGACCGGGACCCCGCCGGGGGAAAAACTTTAAGTGCCACATTAAcatacaa                                                                                            >  2:26651/1‑132 (MQ=25)
                                                    ccgcTGAAGGTTA‑AACACGTCCGCCCACAGGTAATGTCCCCTTCTTATAATCCTGCATGCCCGCGCGGTGGG‑CCCGGCC‑AT‑CATCCCGGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACaa                                                                                      <  4:941229/139‑1 (MQ=255)
                                                      gcTGAAGGTTAAAACT‑GTCCGCCCCCACTCTTTGGTCCTTTCTTATAATTCTGCATGCCCCCGCTGGGGC‑CCGGGAC‑AA‑CATGCCCGGCCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATa                                                                                    <  8:269701/140‑1 (MQ=255)
                                                       cTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑AATGACCGGGACCCGCGCGGGGGAAAACGTGTCAGTGCGACATTCCATGACATTCACCATaa                                                                                   >  5:870607/1‑140 (MQ=255)
                                                          aaGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGCCCCGGACGGGGGAAAACGTTTCAGTGCCCCCATCACTGACATTCACAATaaaca                                                                                >  3:1105915/1‑137 (MQ=39)
                                                              ttATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCGGTC‑CT‑CAATACCGGGCCCCCGCCGGGGGGAAACCTTTCACTTGCGCAATCACTTACACTGACCAAAACAAG‑a                                                                             >  1:1128318/1‑138 (MQ=255)
                                                               tataACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑GCCCGGC‑AT‑CAAGCGCCGGACCCCGCGGGGGGTAAACCTTTAACTGCCACATTACCTGACAGTGACAATAACATG‑aaat                                                                          >  8:513517/1‑140 (MQ=255)
                                                                ataACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGACGGGGGAAAACGTTTCATTGCCACATTCACTGACATTCACCATAACATG‑ATAtt                                                                         >  4:653549/1‑140 (MQ=255)
                                                                 taACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTG‑AT‑AATGTCCGGGACCCGGACGGGGGAAAAAGGTTAATTGACACATTAACTGACATACAAAATAACATG‑ATAtta                                                                        >  4:496823/1‑139 (MQ=255)
                                                                  aaCA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑AT‑CATGGCCGGGCCCCGGCCGGGGGAAAACGTTTCAGTGCCCCCTTCACTGCCATTCACAATAACATG‑ATAtt                                                                         >  1:430712/1‑138 (MQ=38)
                                                                      ccccAGCCCACAATAAATGTTACCTTTTTATAATGCGGAAGTGCCTCTCTGTGCC‑CCCGGTC‑AT‑CATCCCGGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATtcatca                                                                    <  8:287689/137‑1 (MQ=255)
                                                                       gTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTG‑CA‑AATGACCCGGGCACCGCCGGGGGAAAAACTTTAAGTGCCCAATTGACGGACAATAACAAAAACAAG‑ATATTAAacac                                                                   >  4:1108011/1‑140 (MQ=255)
                                                                       gTCAGGCCACACTGAATGTGACCTGCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑CATGACCGGGACCCCGACGGGGGAAAACGTTTAAGTGACCAAATCACAGACATTCAAAAAAACAAG‑ATATtaatcaa                                                                   >  6:474025/1‑139 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AT‑CATGACCGGGGCCCGGGCGGGGGGAAACGTTTAAGTGGCCCATTCACTGTCATTAACAATAACATG‑ATATTAATAACg                                                                  >  7:558170/1‑140 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCAGTGGT‑CCCCGGC‑AG‑GATGACCGGGCCCCGCCCCGGGGCAAAACTTTGACTTGCCCAATAACAGAACATCACAATAACAAG‑ATATtaataaac                                                                  >  7:358244/1‑136 (MQ=255)
                                                                          cgccccaacTTAATGTTACCTTATTATAATTCTCCATGCCCTCTCTGTGGC‑CCCGGCC‑AT‑ATTCCCCTGGCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGa                                                                <  4:1105915/133‑1 (MQ=255)
                                                                          aGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCCGGCGGGGGAAAACCTTTAAGGGCCACATTCACAGACATTCACCAAAACATG‑TTATTCATCAcc                                                                  >  1:562186/1‑137 (MQ=255)
                                                                          aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AA‑AATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACAGTAAATGACATTAACAATAACATT‑ATATtaataaag                                                                  >  1:556417/1‑134 (MQ=255)
                                                                          aGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑AAATACCGGGGCCCGGCCGGGGGGAAACGTTTGAGTGCCCCAATAACTGACATTCACAAAAACATG‑ATATTAATAAcc                                                                  >  1:457895/1‑137 (MQ=255)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTG‑AT‑CATGACCCGGCCCCGGGCGGGGGGAAACGTTTAAGGGCCCCAGTCACGGACATTAACAATAACCAG‑ATATTCATAACGGAGt                                                              >  7:313697/1‑140 (MQ=255)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGTCGGGGGAAAACGTTTAAGTGGCCCATTAACAGACAATGACAATAACATG‑ATATTAATCACGGAGt                                                              >  8:569203/1‑140 (MQ=38)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑CT‑CAAGACCGGGCCCCGGACGGGGGAAAAAGTTTCAGTGCCACATTAACGGACATTAACAATAACATG‑ATATTAATAACGGAGt                                                              >  5:567169/1‑140 (MQ=255)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑AT‑CATGAACGGGACCCGGGCGGGGGCAAACGTTTGAGTGCCCCATTCACTGACAATAACAATAACATG‑ATATTAATCACGGAgg                                                              >  4:799462/1‑139 (MQ=38)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AT‑CAAGACCGGGACCCGGACGGGGGAAAACGTTTCAGAGCCACAATCACTGACATTAACAATAACATG‑ATATTAATCACGGaag                                                              >  5:521126/1‑138 (MQ=255)
                                                                              cacaCTTAATGTGACATTCTTATATTTCTGCATTCCCTCTCTGGGGC‑CCGGGCC‑AT‑CATCCCGGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGa                                                            <  7:877417/140‑1 (MQ=255)
                                                                                cctttaaTGTTACCTTCTTAATTTGCTGCTTGGCCACTTTTTGGG‑CCGGGCC‑AT‑CATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACt                                                          <  8:764079/135‑1 (MQ=255)
                                                                                accttcaTGTGCCCTTCTCATAATGCTTTTTTGCCCCGCTGGGGT‑CCCGGAC‑AT‑CTGGCCCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACt                                                          <  8:877247/134‑1 (MQ=255)
                                                                                 acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AT‑CAAGCACGGGACCCGGACGGGGGAAAACGTTTAAGTGCCACAATCACAGACATTCACAATAACAAG‑ATATTAATAAAGGAATGAcaa                                                         >  7:341809/1‑138 (MQ=255)
                                                                                  tttattttgACCTTCTGATAATTCTGCATTGCCGTTTGGGGGT‑CCCGGCC‑AT‑CATGACGGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTAt                                                        <  4:881846/133‑1 (MQ=255)
                                                                                     aaTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTG‑AT‑AATGAACGGGACCCGGACGGGGGAAAAAGTTTAAATTCCACATTAAATGCCATTCACAAAAACATGAATATT‑ATAACGGAGGGACAATGtt                                                     >  4:356203/1‑140 (MQ=255)
                                                                                       tgtgACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AA‑CATGACCGGGACCCGGACGGGGGAAAACGTTTAAGTGCCCCAATAACTGACAATCACAAAAAAATG‑ATATTAAACACGGAGTGACTATGTTaa                                                   >  7:945103/1‑139 (MQ=255)
                                                                                       tgtgACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑CATGACCCGGCCCCGGGCGGGGGAAAACGTTTCAGTGCCACAATCACTGACATTAACAATAACATG‑ATATTAATAACGGAGTTACTATTTTaa                                                   >  7:950084/1‑139 (MQ=255)
                                                                                        gtgACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGTC‑AT‑CATGACCGGGCCCCCGACGGGGGTAAACGGTTAAGTGCCACATTCACTGACATTAACAATAACATG‑ATATTCAGAACGGAGTGACTAttttt                                                    >  2:122059/1‑137 (MQ=255)
                                                                                          gACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCCGGG‑AT‑CATGACCGGGACCCGGACGGGGGTAAACGTTTCAGTGCCACATTCACTGACAGTGACAATAACATG‑ATATTCAAAACGGAGTGACTATGTTACAGa                                                >  3:470689/1‑140 (MQ=255)
                                                                                           aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑CT‑CATGACCGGGACCCGGACGGGGGGAAAAGTTTCAGTGCCACAATAACTGACATTAAAAAAAACATG‑ATATTAATCAAGGAGTGACGATGTTTAAGAt                                               >  5:482315/1‑140 (MQ=255)
                                                                                           aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGGC‑AT‑AATGACCGGGGCCCGGCCGGGGGCAAACGTTTAACTGCCACATTCACTGACATTAAAAAAAAAATG‑ATATTAAACAAGGACTGCCTATTTTaaataa                                               >  5:482314/1‑135 (MQ=255)
                                                                                                 taaTAATTCTCCATGGCCTCTCTGGGGC‑CCGGGGC‑AT‑AATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                         <  4:969123/138‑1 (MQ=255)
                                                                                                 tGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGCCCGGGCCCCGGACGGGGGAAAACGTTTAAGTGCCACATTCACTGGCATTCACAAAAACATT‑ATATTAAACACGGAGGTACTATGTTACAGATaatagg                                         >  6:826059/1‑135 (MQ=255)
                                                                                                 tGATAATTCTGCATTGACTCTCTGTGGT‑CCCCGTC‑AT‑CATGACCGGGACCCGGACGGGGGAAAAAGTTTAAGTGCCACAATAACTGACATTAACAATAACATG‑ATATTAAAAACGGAGTTACAATGTTACAGATAGta                                           >  2:691528/1‑137 (MQ=255)
                                                                                                      aTTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CAAGACCGGGAACCCGCCGGGGGTAAACGTTTAAGTTGCACAATCACTGACATTCAAAATAACATG‑ATATTAATCACGGAGTGACTAATTTAAAGATAGTCGgcgcgc                                    >  4:801347/1‑140 (MQ=255)
                                                                                                       ttCTGCATTGCCTCTCTGTGGT‑CCCCGTG‑CT‑CAAGCCCGGGACCCGGACCGGGGTAAACGTTTCATTGCCACAATGACAGGCCTTAACAATAACATG‑AGATTACTAACGGAGTGAAGATGGTACACCTTGTAGgcgcg                                     >  2:265518/1‑138 (MQ=255)
                                                                                                       ttCTGCATTGCCTCTCTGTGGT‑CCCCGGC‑AT‑ACTGACCGGGCCCCCGACCGGGGAAAACGTTTAACTGCCCCAATCACAGACATTAACAAAAAAAAG‑ATATTAAAAACCGATTGACTATTTTAAATATAGTAGgcgcgcg                                   >  4:1223822/1‑139 (MQ=255)
                                                                                                                  gccactGTGGTCCCCGGTCAAT‑AATCACGGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctg                            <  2:650826/134‑1 (MQ=255)
                                                                                                                   tctctGTGGT‑CCCGGTC‑AT‑CATTACCGGGACCCGGCCGGGGGAAAACGTTTAAGTGCCACATTCACTGACATTCACAATAACATT‑ATATTCATCACGGAGGGACTATGTTAAAGATAGTCGGCGCGCTGATTctgctg                         >  2:763061/1‑138 (MQ=255)
                                                                                                                                 ggTC‑AT‑CATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTAATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATAGCAGGATTTGcc          >  4:745127/1‑140 (MQ=255)
                                                                                                                                         catGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTATGTTACAGCTAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAtgctt    >  8:557814/1‑140 (MQ=255)
                                                                                                                                          tgcaCCGGCACCCGTACGGGTGACAACGTTTCCTTGCAACATTCACTGACTTTCACAATAACATT‑ATATTCATCACGGAGTGTCTATGTTAAAGATAGTCTGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttct     <  1:1191735/135‑1 (MQ=255)
                                                                                                                                           tGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCg  >  5:445188/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                          
AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGT‑CCCGGTC‑AT‑CATGACCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACATTCACAATAACATG‑ATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTCG  >  NZ_CP022686/4677904‑4678178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A