Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F2 I0 R1 166 6951.0 8192394 98.4% 8061315 136.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,041 A→C 14.4% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0410AC14.4% 67.4 / 5.7 89intergenic (+39/‑82)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (68/4);  new base C (3/9);  total (76/13)
Fisher's exact test for biased strand distribution p-value = 3.68e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01

AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTC  >  NZ_CP022686/4677904‑4678177
                                                                                                                                              |                                                                                                                                          
aaTATCCCCCTGGGCGTTCAGGCCGGTTATGCCCCCTGCATCAACGGCAGCAGCGCTTAAGGTTA‑AACACGTCCGGACACACGGAATGTGACCTTCTGATAATTCTGAA‑TTCCCTCTCTGGGGTCCCGGT‑CAT‑CATG‑c‑cc                                                                                                                                         <  2:308699/140‑1 (MQ=21)
  tatCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑GAT‑CATG‑A‑CCgg                                                                                                                                       >  6:348335/1‑140 (MQ=255)
       cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑ACGGGAccc                                                                                                                                  >  4:587056/1‑140 (MQ=255)
          tGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑TTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGTTCCCCCG‑CCT‑AAAG‑A‑CCGGGAcccagg                                                                                                                               >  5:338037/1‑137 (MQ=18)
          tGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTTATAATTCTGCT‑TTGCCTCTCTGTGGTCCCGGA‑CAT‑CATG‑A‑CCGGGACCCGGa                                                                                                                               <  2:917962/140‑1 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAT‑CCTG‑A‑CCGGGACCCGGGCg                                                                                                                             >  6:817766/1‑140 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CAAG‑A‑CCGGGACCCGGACg                                                                                                                             >  7:80691/1‑140 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑A‑CCGGGACCCGGAcc                                                                                                                             >  5:203371/1‑139 (MQ=255)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑AAT‑CATG‑A‑CCCGGACCCGGGCg                                                                                                                             >  6:931224/1‑140 (MQ=255)
            ggCGTT‑AGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCGCTCTGTGGTCCCCGT‑CAT‑CATG‑A‑ACGCGGCACGGGCgg                                                                                                                            >  7:254398/1‑140 (MQ=21)
                 tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACAGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CCA‑CCTT‑A‑CCCGGACCCGGACgggggg                                                                                                                        >  5:161213/1‑139 (MQ=25)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCATCAGCGCAGAAGGTTATAACA‑GTAAGGCCACACTGAATGTGACCTTCTGATAATTCTTCA‑TTTCCTCTCTGTGGTACCGGA‑CAT‑GAAG‑A‑ACAGGACCCGCACGGGGTaa                                                                                                                       >  2:404985/1‑140 (MQ=21)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGCAGGTTATAACA‑GTCAGGCCACACTGAATGAGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑CAT‑CATA‑A‑CCTGGACCCGCGCgggggta                                                                                                                       >  5:1006503/1‑138 (MQ=25)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTTCCTCTCTGTGGTCCCCGG‑CAT‑AAAG‑A‑CCCGGCCACGGTCCGGGGaa                                                                                                                       >  5:356294/1‑140 (MQ=21)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTGCCGGG‑GAT‑CAGT‑G‑GCGGGCACCGGCCGGGGGac                                                                                                                       >  5:45856/1‑139 (MQ=18)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGaa                                                                                                                       >  4:575815/1‑140 (MQ=255)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑CAT‑CATT‑ACCCGGGCCCCGGCCGGGGGa                                                                                                                        >  5:890572/1‑140 (MQ=21)
                     gCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCATGCCACACTGAATGTGACATTCTTATAATTCTGCA‑GTGCCTCTCTGTGGTCCCGGG‑GCT‑CATG‑T‑CCGGGACCAGGGCGGGGGAAAAc                                                                                                                    >  6:220222/1‑140 (MQ=21)
                         gTTATGCCCACAGCATCAACGGAAGCAGCGCTGAAGGTTATAACA‑GACAGGCCACACTGAATGTGCCCTTCTGATAATTCTGAA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑CAAT‑A‑CCGCGCACCGGCCGGCGGATAACCttt                                                                                                                >  3:11209/1‑140 (MQ=21)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑ACT‑ACTG‑A‑CCGGGACCCGGCAGGTGGAAAACGttta                                                                                                               >  4:238319/1‑139 (MQ=25)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑CAAG‑A‑CCCGGTCCCGGCCGGGGGgaaaactttg                                                                                                               >  5:802790/1‑134 (MQ=25)
                               cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGTGCTG‑CTTG‑A‑CGGGGACCCGCCCGGGGGAAAA‑GTTTTAGTgg                                                                                                          >  7:526968/1‑139 (MQ=21)
                               cccACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGTGCAA‑GTGC‑A‑CCCGCACCCGG‑CGGGGGAAAACGTTTTAggtg                                                                                                          >  7:169819/1‑137 (MQ=18)
                                   caGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CCT‑CCTT‑A‑CCCGGCCCCGAACGGGGGAAAACGGTTAACTGccgac                                                                                                      >  7:504323/1‑137 (MQ=25)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAT‑CATT‑A‑CCGGGTCCCGGACGGGGGAAAACGGTTAAGTGCCACAt                                                                                                     >  1:316038/1‑140 (MQ=37)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAT‑CATG‑G‑CCGGGCCCCCGACGGGGGCGAACCTTTCAGTgggacca                                                                                                     >  1:325855/1‑134 (MQ=25)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑AAT‑AATG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTAAGTGGCACAt                                                                                                     >  8:815968/1‑140 (MQ=37)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCCG‑CAT‑AATT‑A‑CAGGGACCCGCACGGGGGGAAAAGTTTCAGTGCCACAt                                                                                                     >  5:386409/1‑140 (MQ=25)
                                         aacgggCAGCAGGGCTTATGTTTAAAACT‑GTCCGCCCAAAATTATTGTGACTTTCTTAAATTTCTGCT‑TTGCCTCTCTGGGGCCCCGGC‑CAT‑CTTC‑C‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACt                                                                                                <  4:560033/136‑1 (MQ=255)
                                              gcagcaGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CCT‑CAAG‑A‑CCCGGACCCGGCCGGGGGGAAACGGTTAAGGGCCACAATAACAGAcca                                                                                           >  5:134865/1‑138 (MQ=25)
                                               cagccgCGCTGATGGTTAAACCC‑CCCAGCCCCCACTGATGGTGCCCTCATCAAAATTGTGCA‑TTCCCTCTCGGGGGCCCGGCC‑CAC‑CCTC‑C‑CGGGGCCCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAt‑t                                                                                         <  4:575957/140‑1 (MQ=255)
                                                agcagcGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑G‑CCGGGGCCCCGTCGGGGGAAAACTTTTAAGTGCCACATTCACTTACAT‑Tc                                                                                        >  3:554646/1‑140 (MQ=37)
                                                agcagcGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑CAAG‑A‑ACGGGACCCCGACGGGGGAAAACCTTTCAGTGCCACATTCACAGACAt‑ta                                                                                        >  7:385568/1‑139 (MQ=37)
                                                agcagcGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTATGCA‑TTGCCTCTCTGTGGTCCCCGT‑AAT‑AATG‑A‑CCGGGACCCGTCCGGGGGAAAACGTTTCAGTGGCACATTCACTGACAt‑ta                                                                                        >  3:574268/1‑139 (MQ=37)
                                                 gcagcGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑GATGCATC‑C‑CCGGG‑GCCGGCCGGGGGGGAACGATTGAGGGGCCCATTCCCGGACAt‑tga                                                                                       >  1:166130/1‑138 (MQ=16)
                                                  cagcGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑CAT‑CATG‑ACCCGGGCCCCGGCGGGGGGAAAACG‑TTAAGTGCCACAATAACAGACaatgaa                                                                                       >  1:11985/1‑135 (MQ=25)
                                                        tGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑A‑CCGGGCCCCGGACGGGGGAAAACGTTTAAGTGCCACATTCACTGACAT‑TCACAATaaa                                                                                >  2:858756/1‑139 (MQ=255)
                                                          aaGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑CAAT‑A‑CCGGGCACCGGCCGGGGGCAAAAGGTTAAGTGCCCCCTTAACTGACCT‑TCACAATAACAt                                                                              >  4:506310/1‑140 (MQ=255)
                                                            ggTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAA‑CATG‑A‑CCGGGCCCCGGAACGGGGCAAACCTTTAAGTTCCACATTCAAGGACAT‑TACCAATAAAATGa                                                                            >  6:285090/1‑140 (MQ=25)
                                                               tataACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑AAT‑CATGTA‑CCGGG‑CCCAGGCAGGGGCAAACGGTTGAGGGCGACAATTGCTG‑CATGTCACAATAAAATAatat                                                                         >  3:301425/1‑140 (MQ=255)
                                                                 taACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGGCGGGGGGAAACGTTTCAGGGCCATATTAACAGCCAT‑TCACAATAACATGATAtta                                                                       >  3:626801/1‑139 (MQ=255)
                                                                 taACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑GAT‑CATG‑A‑CCGGGGCCCGGCCGGGGGTAAACGTTTCAGGGCGCCAATCCCTGACAT‑TCACAATAAAATGATAttt                                                                       >  3:818384/1‑139 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑CAT‑CAAG‑A‑CCGGGACCCGGGCGCGGGAAAACGGTTAAGTGCGACATTAACTGACAT‑TGGCAATAACATGATAttaaacaag                                                                 >  4:463540/1‑133 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAT‑ACTG‑G‑CCGGGCCCCGGACGGGGGTAAACGTTTCAGTGGCACATTCACGGCCAA‑TCACAATAAACAGATTTTAATCACg                                                                 >  8:661671/1‑140 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAT‑AATT‑A‑CCGGGGCCCGGGCGGGGGTAAACGTTTGAGTGCCACATTCAAAGACAT‑TAACAAAAACAAGATAttaaaaaaa                                                                 >  5:327959/1‑133 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑GAT‑GATG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGGCAACTTCACTGGCAT‑TCACAATAACATGATATTGATAACg                                                                 >  3:736354/1‑140 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑GAT‑CATG‑C‑CCGGGACCCGGGCGGGGGGAAACGTTTGAGTGCCACATTGACTGACAT‑TCACAATAACATGTTATTGATCACg                                                                 >  5:758961/1‑140 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑A‑CCGGGGCCCGGCCCGGGGGAAACGTTTGAGTGGCACATTGACGGGCAT‑TAACAAAAACAATAAATtcatcaag                                                                 >  5:78387/1‑138 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑AGA‑CATG‑A‑CCGGGACCCGGACGGGGGATAACGTTTAAGTGCCACCTTGACGGACAA‑TCCAAATAACATGATATtaatcaag                                                                 >  4:192732/1‑138 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCACGGG‑CAT‑AATG‑A‑ACGGGGCCCGGCCGGGGGAAAAACTTTGAGTGCCCCATTGACTGGCAT‑TAAAAATAACATGATATTAATAACg                                                                 >  5:285164/1‑140 (MQ=255)
                                                                        tCAGGCCACACTGAATGTGACCTTCTGATAAATCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGAAAAAGTTTCAGATCCACATTAACAGACAT‑TGACAATAACATGATAATCATAACg                                                                 >  2:844860/1‑140 (MQ=255)
                                                                          aGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑A‑CCGGGACCCGGCCGGGGGAAAACGTTTCAGTGGCCCAATATCGGGCAA‑TGACAATAACAATATATTAATAACCGa                                                               >  2:131924/1‑140 (MQ=255)
                                                                          aGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑CAAG‑C‑CCGGGACCCGGACGGGGGAAAACCTTTCAGTGCCCCATTCACTGACAT‑TCACAATAACAAGATATTAATCACggc                                                               >  7:574409/1‑139 (MQ=255)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CCT‑CATG‑A‑CCGGGGCCCGGGCGGGGGTAAACGGTTAAGTGCCCCCTTCACTGGCAG‑TCACAATTACAAGATATTCATAACGGAgg                                                             >  4:481484/1‑139 (MQ=255)
                                                                            gCCACACTGAATGTGACCTTCTGATAATTCAGCA‑TTGCCTCTCTGGGGGTCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGt                                                             <  5:81752/140‑1 (MQ=255)
                                                                            cccACCCTTAGTGTGACATTCTTAAATTTCTCCT‑TTGCGTCGGTGTGGCCCCGGT‑CCT‑CTCG‑C‑CGGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGt                                                             <  6:158056/139‑1 (MQ=255)
                                                                              cacaTTTAATGTTACCTTTTTATATTTCTGCA‑TGGCCGCTCTGGGGGCCGGGT‑ACT‑CATG‑C‑CGGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACTT‑TCACAATAACATGATATTCATCACGGAGTGa                                                           <  8:385568/140‑1 (MQ=255)
                                                                              aacacTTATTGTCACTTTATTATAATTCTCCT‑TTCCCTCTCTGTGGCCCCGGC‑CAT‑CTTC‑C‑CGGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGa                                                           <  8:55252/139‑1 (MQ=255)
                                                                                cacTTAATGTTACTTTCTTAT‑ATTCCGTATTTGCTGCTCTGGGGCCCCGGT‑AAT‑CATC‑C‑CGGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACt                                                         <  8:60205/140‑1 (MQ=255)
                                                                                 acTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑AATG‑A‑CCCGGACCCGGACCGGGGAAAACGATTAAGTGCCACCATAAAAGACAT‑TAACAAAAACATTATATTCCTAAAGGAGTggcaa                                                        >  6:86742/1‑136 (MQ=255)
                                                                                  cTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑GAT‑CATG‑A‑CCGGGCCCCGGCCGGGGGGAAACGGTTCAGTGGCACATTAACTGACAT‑TAACAAATACATGATATTAATAACCGAGTGACTAt                                                       >  7:55252/1‑140 (MQ=255)
                                                                                    gAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGCAAACGTTTCAGTGCCACATTAACTGACAT‑TCACAATAAAATGATATTAATCACGGAGTGAAGATGt                                                     >  4:634675/1‑140 (MQ=255)
                                                                                      ttggcaCCTTCTTATAATTCTGCA‑TTCCGTCTCTGTGGCCCCGGC‑CAT‑AATC‑A‑CCGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTa                                                   <  6:257795/135‑1 (MQ=255)
                                                                                          gACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑GAT‑CAAG‑A‑ACGGGACCCGGACGGGGGAAAAACTTTCAGTGCCACAATAACAGACAT‑TAACCAAAACATGATTTTAATCACGGAGTGACTATTTTACata                                               >  7:121802/1‑138 (MQ=255)
                                                                                           aCCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGGAAACGGTTCAGTGGCCCAGTGACTGCCAT‑TCACAAAAACATGATTTTCATCAGGGAGTGGCTCTGTTACAGAt                                              >  7:651692/1‑140 (MQ=255)
                                                                                           aCCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTAAGGGCCACATTGACTGACAT‑TAACAATAACATGATATTAATAACGGATTGACTATGTTAAAGAt                                              >  8:416403/1‑140 (MQ=255)
                                                                                           aCCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGT‑CAT‑CAAG‑A‑CCGGGACCCGGACGGGGGATAACGTTTCAGTGCCACAATAAAAGACAT‑TAACAATAACAAGATATTAATAACGGAGTGACTATGTTACAGAt                                              >  3:468396/1‑140 (MQ=255)
                                                                                           aCCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑GAT‑CATG‑A‑CCCGGACCCGGACGGGGGTAAAAGGTTGAGTGCCACAATAACAGCAAA‑TAACAATAACAAGATATTACACACGGAGTGCGAATGTTACAGAt                                              >  4:155755/1‑140 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATT‑A‑CCGGGACCCGGAAGGGGGAAAAAGTTTCAGTGACAAATTAACTGACAT‑TCACAAAAACATGATATTCAACACGGAATGACTTTGTTACAGATAGTc                                          >  8:652014/1‑140 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGGACCCGACGGGGGAAAACGTTTGAGTGCCCCAATCACTGACCT‑TAACAATAAAATGAGATTCATCCCCGCGTGACTATTTTACAGATAGtg                                          >  2:735582/1‑139 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGCCCCGGACGGGGGTAAACGTTTCAGTGGCACATTCACTGACAT‑TCACAATAACATTATATTAATCACGGCGTGACTATGTTACAGATAGta                                          >  7:898089/1‑139 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCCGGACCCGGACGGGGGGAAAAGTTTAAGTGCCCCCTTCACTGAGAT‑TCAAAATAACATGATATTAATAACGGAGTGACTATGTTACAGATAGtg                                          >  3:49625/1‑139 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑AATT‑A‑CCGGGACCCGGACGGGGGAAAACGGTTCCTTGCCACATTACGTGCAAT‑TCACCATAAAAAGATATTCATAAAGTAGTGCCTTTGTTAAAGATATTc                                          >  8:962464/1‑140 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑GAT‑CATG‑A‑CCCGGACCCCGCCGGGGGGAAAACTTTAAGTGCCACAATAACTGACAT‑TAACAATAACATTATATTAATAACGGAGTGACTATGTTACAGATaatt                                          >  1:133693/1‑137 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGG‑CAT‑CATG‑A‑CCGGGCCCCGGCCGGGGGAAAACGTTTCAGTGCCACATTCAAATAAAT‑TCACAATAACATTATATTAATCAAGGAGTGCCTATGTTACAGATAGta                                          >  4:115498/1‑139 (MQ=255)
                                                                                               tCTGATAATTCTGCA‑TTGACTCTCTGTGGTCCCGGT‑CAT‑CATT‑A‑CCGGGACCCGGGCGGGGGAAAACGTTTAAGTGACCAATTCACTGACAT‑TAACAAAAAAATGATATTCATCAAGGAGTGACTATTTTAGATATAGTc                                          >  8:406504/1‑140 (MQ=255)
                                                                                                   aTAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑GAT‑CATG‑A‑CCGGGGCCCGGACGGGGGGAAACGGTTAAGTGGCACATTCACTGACAT‑TAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                      >  4:1011979/1‑140 (MQ=255)
                                                                                                   aTAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGCCGGGGGCAAACGTTTAAGTGCCACATTCACTGACAT‑TCACAAAAACATGATATTAATCACGGAGTGACTATGTTTAAGATATTAGgcg                                      >  4:948873/1‑140 (MQ=255)
                                                                                                        tCTGCA‑TTGCCTCTCTGTGGTCCCCGG‑CAT‑CAAG‑A‑CCGGGACCCGGACGGGGGCAAACCTTTAAGGGCCCCATTCAAATACAA‑TCACAAAAACATTATATTCATCACGGAGTGACTATGTTAAAGAAAGTAGGCCCGCtt                                 >  6:342029/1‑139 (MQ=255)
                                                                                                          tGCA‑TTGCCTCTCTGTGGTCCCGGG‑ACG‑CAAT‑A‑ACGGGACCCGGACGGGGGAAAACGTTTCAGTGACACATTCACAGCCAT‑GAAAAATAACATTATATTCAAAACGGTATGGCGATGTGTCAGATATTCGGCGCGCAGAt                               >  8:143757/1‑140 (MQ=255)
                                                                                                               ttGCCTCTGTGTGGCCCGGGC‑CAT‑CATG‑A‑CGGGTACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctg                           <  2:228552/140‑1 (MQ=255)
                                                                                                                 cccTCTCTGGGGTCCCGGA‑CAA‑CATG‑C‑CGGGCACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctgct                         <  2:1051248/139‑1 (MQ=255)
                                                                                                                  ccTCTCTGTGGTCCCGGT‑CAA‑CATT‑A‑CCGGGTCCCGGACGGGGGGAAACGTTTCAGTGCCACCTTCACTGACAT‑TCACAATAACATGATATTAATAACGGAGTTACTATGTTACATATAGTCGGCGCGCTTATTcttctg                        >  8:89574/1‑140 (MQ=255)
                                                                                                                       ctGTGGTCCCCGT‑CAT‑CATG‑A‑CCGGGGCCCGGACGGGGGGAAACGTTTGAGTGCCACATTGACTTACAT‑TCACAATAACAAGATATTAATCACGGAGTGGCTATGGTTCAGATAGTCGGCGCGCTGATTCTGGGGATCGc                   >  6:471625/1‑140 (MQ=255)
                                                                                                                          ggttCCGGGT‑CAT‑CATG‑C‑CTGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAgg                <  8:77124/137‑1 (MQ=255)
                                                                                                                            gTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGCCGGGGGGAAACGTTTAAGTGCCACATTGACTGACAT‑TAACAATAACATGTTATTCATCACGGAATGACTATGTTACAGATAGTCGCCGCGCGGATTCTGGCGCTCGCAggct              >  8:708785/1‑138 (MQ=255)
                                                                                                                            gTCCCCGT‑GAT‑CATG‑A‑CCGGGACCCGGACGGGGGAAAAACTTTAATTGCCACATTAACATACAT‑TAACAATAAAAATATATTAATAAAGGAGTGACAAATTTACAGAAATTCGGCGCGCGGAATATTCTGATCGCACGAt              >  2:935669/1‑140 (MQ=255)
                                                                                                                                       t‑catG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGCCAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGTTAGTCGGCGCGCTGCTTCTGCTGATCGCAGGATTTGCCAttcg    >  6:30076/1‑139 (MQ=255)
                                                                                                                                         catG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttctt   >  8:40905/1‑140 (MQ=255)
                                                                                                                                          atG‑C‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc  <  4:806822/140‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                          
AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACA‑GTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCA‑TTGCCTCTCTGTGGTCCCGGT‑CAT‑CATG‑A‑CCGGGACCCGGACGGGGGAAAACGTTTCAGTGCCACATTCACTGACAT‑TCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTC  >  NZ_CP022686/4677904‑4678177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A