Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F35 I0 R1 271 8871.0 10354886 98.4% 10189207 136.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 4,678,041 A→C 17.6% intergenic (+39/‑82) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0226864,678,0410AC17.6% 79.2 / 2.7 92intergenic (+39/‑82)agn43/CIW80_RS23450autotransporter adhesin Ag43/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (63/7);  new base C (10/7);  total (78/14)
Fisher's exact test for biased strand distribution p-value = 4.96e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTC  >  NZ_CP022686/4677904‑4678177
                                                                                                                                          |                                                                                                                                         
aaTATCACCGTGGGCGTTCAGGCCGGTTATGCCCACAGAATCAACGGCAGCTGCGGTGATGGTTATAACATTCCGGCCACACTTAATGTTACCTTCTTATAATTCTGCATGGCCTGTCTTTGGGCCCGGTCATCAT‑GAcc                                                                                                                                         <  4:1269739/140‑1 (MQ=21)
  tatCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTTATAATTCTGCATTGCCTCTCTGTGGTCCCCGTAATAAT‑GACCgg                                                                                                                                       >  2:183325/1‑140 (MQ=255)
       cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACTGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACCCTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGCGATCCT‑GACCGGGAccc                                                                                                                                  >  1:645469/1‑140 (MQ=21)
       cccTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCATCAT‑GACCCGGcccc                                                                                                                                  >  6:1176263/1‑140 (MQ=255)
           gggCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCGGAAGGTTATAACAGTCAGGCCACACTGAATGTGTCCTTCTGATAATTCTGCATTGCGTCTCTGTGGTCCCCGTCCTCAT‑GGCCCGGCCCCggcc                                                                                                                              >  7:1117848/1‑138 (MQ=25)
            ggCGTTCAGGCCGGTTATGCCCACAGCATCACCGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATAGCCTCTCTGTGGTGCCGGTCATCAT‑GCCCGGGACCCGGACg                                                                                                                             >  1:266886/1‑140 (MQ=21)
                 tCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCATCAT‑GACCGGGCCCCGGCCgggggg                                                                                                                        >  2:687667/1‑139 (MQ=255)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCCGACGGGGGaa                                                                                                                       >  6:1146965/1‑140 (MQ=255)
                  cAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCAAAAA‑TACCGGGACCCCGCCGGGGGaa                                                                                                                       >  3:194891/1‑140 (MQ=21)
                     gCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGTCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTACCCGGGATCAT‑GACCGGGACCCCGCCGGGGGgaaaa                                                                                                                    >  1:97765/1‑139 (MQ=25)
                     gCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGaaaaa                                                                                                                    >  7:753067/1‑139 (MQ=255)
                     gCCGGTTATGCCCACAGCATAAACGGCAGCAGCGCTGAAGGTTATACCAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTATGAATTGACTCTCTGTGGTCCCCGTAAACAG‑TACCGTGACACGGACCGGGGaaaca                                                                                                                    >  1:91422/1‑138 (MQ=18)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGCCCCGGACGGGGGAAAACGttta                                                                                                               >  6:766433/1‑139 (MQ=255)
                          ttATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGAACTTCTGATAATTCTGCATTGCCTCTCTGTGGTCACGGTCATCAT‑TAACGGGACACGGTCGGGGGTAAACGttta                                                                                                               >  6:915684/1‑139 (MQ=25)
                              ccccACACAATAAAGGGCGCCAGCGATGTTGGTTAAAAAAGCCAGCCAACCCTGATTGGTACCTTTTTATAATTCTGCAGGGCCTCGCTTGGGCCCCGGACATCAT‑CACGGGGACCCGGACGGGGGAAAACGTTT‑CAGTg                                                                                                          <  2:750793/139‑1 (MQ=255)
                                   caGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCcaca                                                                                                     >  1:1053811/1‑140 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGAACCGGACGGGGGAAAACGGTT‑CACTGCCAAAt                                                                                                    >  2:1047020/1‑140 (MQ=38)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCTCTCATCAA‑GACCGCGGCCCGGACAGGGGTAAAAGTTT‑AAGAGCcaaca                                                                                                    >  5:1197286/1‑137 (MQ=25)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATAAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑AAGTGCcacaa                                                                                                    >  2:349840/1‑139 (MQ=255)
                                    aGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCAA‑GACCGGGACCCCGACGGGGGTAAACGTTT‑CAGTGGcacaa                                                                                                    >  1:36659/1‑139 (MQ=37)
                                        tCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACTCAGAATGTGACCTACTGATAATTCTGCTTTGCCTCTCTGTGGTCCCCGTGATCAT‑GACCGGGCGCCGGAAGGGGGGAAACGGTT‑CAGGGCCACATTAAc                                                                                                >  8:196806/1‑140 (MQ=25)
                                              gcagcaGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTGCACAT‑GGCCGGGCCCCGGCCGGGGGGAAACGTTT‑AAGGGCCCCATTAAcggccaa                                                                                          >  3:840870/1‑134 (MQ=21)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCCTCAA‑GACCGGGTCCCCGACGGGGGAAAACGTTT‑AAGTGGCAAATTCACAGACAtta                                                                                        >  3:44408/1‑139 (MQ=37)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGCCGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACAtta                                                                                        >  2:770282/1‑139 (MQ=255)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGGATAAT‑GACCGGGGCCCCGACGGGGGAAAACGTTT‑CAGTGCCACAATCACTGCAATTc                                                                                        >  4:450381/1‑140 (MQ=37)
                                                agcagcGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCATCAT‑GACCGGGACCCGGACCGGGGAAAACGTTT‑AAGTGCCACATTCACTTACAtta                                                                                        >  2:280224/1‑139 (MQ=37)
                                                    ccgcTTATGGTTAAAACAGTCAGGCCCCAGTCAATGTCACCTTCTTATAATTCTCCATTCCCACTCTGTGGCCCCGGGCAACAT‑CCCCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACaa                                                                                    <  4:944962/139‑1 (MQ=255)
                                                       cTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGCTAAACAA‑GAACGGGCCCCCGACGGGGGCAAAAGTTT‑CAGTGCCACCATAACAGACATTAACAaaaa                                                                                 >  2:470143/1‑140 (MQ=255)
                                                           tgattATACCAGGCAGGCCACACTAATGGTGACCTTCTTATATTTGCCATTTCCCTCTCTGGGGTCCGGGTACTCCT‑GACGGGCACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATg                                                                             <  8:299488/137‑1 (MQ=255)
                                                               tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGGATTGCCTCTCTGTGGTCCCGGTGATCAT‑GCACGGGACCCGGGAGGGGGAAAAGCGTT‑AAGTTGCACATTAACTGACATTACAAATAAAATGatat                                                                         >  8:68188/1‑140 (MQ=21)
                                                               tataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTTGTCCCGGGCATCAT‑GACCGGGACCCCGACTGGGGAAAAAGTTT‑CAGGGACACAATAACGGACATTAACAATAAAATGatat                                                                         >  8:363169/1‑140 (MQ=37)
                                                               tataACAGTCAGGCCACACTGAATGTGACCATCTGATAATACTGCATTGCCTCTCTGTGGTCCAGGTCCACAT‑GACCGGGACCCCGCCGGGGGAATACGTTT‑AAGTGACACATTAACAGAAATTAACAATAAAAAGatat                                                                         >  4:256672/1‑140 (MQ=255)
                                                                ataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTGCCGGGGCTCCT‑GTCCGGGGCCCGGGCGGGGGGAAACGTTT‑CAGTGCCCCCTTAACTGGCAGTGACCATAACATGATAtt                                                                        >  6:897488/1‑140 (MQ=255)
                                                                ataACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGGATAAT‑GTCCGGGCCCCGGGCGGGGGTAAACGTTT‑CAGTGCCACATTAAAGGACATTAACAATAAAAAGATAtt                                                                        >  3:1284004/1‑140 (MQ=255)
                                                                 taACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTGATCAT‑GACCCGGCCCCGGGCGGGGGCAAAACTTT‑CAGTGCCACCTTGACAGGGATTAACTATAACCTGTTAtta                                                                       >  7:379428/1‑139 (MQ=255)
                                                                      gTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCAGCAA‑GCCCGGGGACCGGGAGGGGGAAAACGTTTACTTTGCC‑CATTCACGGACATTGCCAATAACAAGATAttaaaaac                                                                  >  1:984387/1‑134 (MQ=255)
                                                                       tccgcccACACTTATTGTGACCTTATTAAAATTCTGCATTGCCTCTCTGTGGTCCCGGTCAACAT‑GCCGGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACg                                                                 <  2:1053811/135‑1 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGCCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGGAAACGGTT‑CAGTGCCACAATCACGGACAGTACCAATAACAAGCTTTtcatacag                                                                 >  5:1015892/1‑136 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTGCCGGGGATAAA‑GCCCGGGCCCCGGCAGGGGGGAAACCGTT‑CAGTTCCACAATGACTGACGATAACAATAACAAGAAAttgaataag                                                                 >  7:1196678/1‑133 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTGCCCGGGATCATGGAACGGGCCCCGTCGGGGGGAAACCGTTT‑‑AGGGGCCCAGTCACGGCCATTGACAATAAAAAGAAAttgaaaacc                                                                 >  8:670644/1‑133 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTGCCAGTCATCAA‑GACCCGGACCCGGACGGGGGAAAACCTTT‑CCGTGCCCCATTCACAGACATGCACAAAAACAAGATATTCATCACg                                                                 >  4:801393/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTGATCAA‑GCCCCGGACCCGGCCGGGGGAAAACCTTT‑GAGTGCAACAATGACTGGCATTAAAAAATACATTATATTCATCACg                                                                 >  7:1196676/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTGATAAT‑GACCGGGACCCGGCCGGGGGAAAACGTTT‑CAGTGGCACATTCACTGACATTAACAATAACATGATATTCATAAcc                                                                 >  7:286434/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCCT‑GACCGGGACCCGGACGGGGGGAAACGGTT‑CAGTGCCACATTCCCTGACATTCACAATAACATGATATTCATCACg                                                                 >  2:931852/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GCCCCGGACCCCGACGGGGGAAAACGTTT‑CAGTGGCCCATTCACAGACATTCACAATAAAATGATAttactcaag                                                                 >  6:718857/1‑133 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGGCGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTAATAAcc                                                                 >  2:817039/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGGAAACGTTT‑CAGTGCCACATTCACTGACATTCTCAATAACATGATATTCATCACg                                                                 >  4:933316/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATAACg                                                                 >  2:1114138/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGAAAACGGTT‑CAGTGCCACATTCACTGACATTAACAATAAAATGATATtcatcaag                                                                 >  4:1166401/1‑138 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGGATCAT‑GACCCGGACCCGGACGGGGGTAAACCTTT‑CAGAGCCACATTCACTGTCATTCTCCATAACAGGTAATTCATCACg                                                                 >  2:562451/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCTTCAT‑GACCCGGCCCCGGCCGGGGGAAAACGGTG‑CCGTGCCCCCGTCAAGGCCCATCACAAAAACAAGATATTCATAAcc                                                                 >  6:672745/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCCTAAT‑GACCGGGCCCCGGACGGGGGAAAACGTTT‑CACTGCCACCATAACTGACATTCACAATAACATGATATTCATCAcc                                                                 >  6:450795/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCAT‑TACCGGGACCCGGACGGGGGAAAACGTTT‑ACGTGCCACATTAACTGAAATTCAAAAAAACATGATATTCATAAca                                                                 >  7:73242/1‑139 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCAT‑GACCGGGACCCGGCCGGGGGTAAAAGTTT‑TAGTGGCCCATTCACGGACATTAACAATAAAATGTTATtaataaag                                                                 >  1:64365/1‑136 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCAT‑GACCCGGACCCGGACGGGGGTAAACGTTT‑GAGTGCCACCTTCACTGACAATAACAATAACAAGCTATtcaaaacg                                                                 >  2:863341/1‑135 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATCAT‑GTCCGGGCCCCGGCCGGGGGAAAACGTTT‑CAGTGCCACATTAACGGACATTAACAATAACATGATATtaataaag                                                                 >  1:1048699/1‑136 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGGCATCAT‑GCCCGGGACCCGGCCGGGGGTAAACGTTT‑AAGTGCCACCTTAACTGACATTAACAATAAAATGATATTAATAACg                                                                 >  4:242462/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCTGATAATTCTGAATTGCCTCTCTGTGGTACCGGTCCTGAT‑GAACGGGAACCCGCAGGGGGAAAAAGTTT‑CAGTGCCACATTCAAATACATTCAAAATAAAAATATATTCATAACg                                                                 >  2:1180169/1‑140 (MQ=255)
                                                                       tCAGGCCACACTGAATGTGACCTTCAGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGAACCCGCCGGGGGCAAAAGTTT‑CAGGGCAACATTCAAGGACAGTCAAAATAACATTATATTAATCAcc                                                                 >  4:905360/1‑139 (MQ=255)
                                                                           gCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGGCCCGGCCGGGGGTAAACGTTT‑GAGGGCCACATTCACGGCCATTCACAATAACATGATTTTAATAACGGAGt                                                             >  2:169071/1‑140 (MQ=38)
                                                                             cacaTTTAATGTGACATTCTTAAAATCCTGCATTGCCTCTCTGGGGCCCCGGTCATCAT‑CCCCGGTACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                           <  4:1258431/140‑1 (MQ=255)
                                                                             cacaCTGACTTTTACCTTTTTATAATTATGCTTTGCTTCTGTTGTGTCCGGGGCTACAT‑TCCCGGGACCCGGACGGGGGAAAACGTAT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                           <  8:580956/140‑1 (MQ=255)
                                                                             aaaacTTAATGTGACATTCTTATAATTCTGCTTTGCCTCTCTGTGGTCCCGGTAATCAT‑CACCGGTACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGa                                                           <  6:845401/137‑1 (MQ=255)
                                                                               ccttGAATGGTACATTCTGATTATTCTGATTTGCCTCTCTTGGGGCCCGGGCATCTT‑GACGGGTACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACt                                                         <  6:1191937/137‑1 (MQ=255)
                                                                               cacTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGCCGGGGGAAAACGTTT‑AAGTGCCCCATTAACTTTCATTAACAATAACATTATATTAATCACGGAGTGACt                                                         >  3:1258431/1‑140 (MQ=255)
                                                                                acTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCCGCCGGGGGCAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACAAGATATTCATCACGGAGTGACTa                                                        >  3:932725/1‑140 (MQ=255)
                                                                                   ttaTGTCACTTTCTTATAATTGTGCTTTCCCTCTGGGGGGCCCCGTCAATCAC‑GCGGGGGCCCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGt                                                     <  2:1029901/138‑1 (MQ=255)
                                                                                   tatggtgACGTTCTGATATTTCTGCTTTGCCTTTATGTGGCCCCGGTATTCTT‑GCCGGGGACCCGGACGGGGAAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGt                                                     <  2:535055/136‑1 (MQ=255)
                                                                                     aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑TCCCGGGACCCGGACGGGGGAAAACGATT‑AAGTGCCCCATTAACTGACATTCACAAAAACATGTTATTCATCCCGGAGTGGCTATGTTa                                                   >  3:800157/1‑140 (MQ=255)
                                                                                     aTGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAA‑TACCGGGCCCCCGCCGGGGGAAAACGTTT‑CAGTGCCACATTAACTGAAAATAACAATAACATGATATTAATAACGGAGGGACTATGttt                                                   >  5:1145133/1‑139 (MQ=255)
                                                                                      tgtgACCTTCTGATAATTCTGCATTGCCTCTCTGTTGTCCCGGAAATCAT‑GACAGGGACCCGGGCGGGGGAAAACGTTT‑AAGTGCCACATTGACTTAAATTAACAAAAAAATGATTTTAATCACAGAGTGACTATGATaa                                                  >  4:1219953/1‑139 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGCCCCGGCCGGGGGAAAACGTTT‑CAGTGCCACCTTAACTGACATTCACCATAACATGATATTCATCACGGAGTGGCTATGGTACAGAt                                              >  4:992461/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCCTCCT‑GACCGGGACCCGGGCGGGGGGAAACGGTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATAACGGAGTGCCTATGTTACAGAt                                              >  8:954632/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGGCATCAT‑GAACGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCCCATTCACGGCCAGTCACAAAAACATGAGTTTCATCACGGAGTGACTATGTTTCAGAt                                              >  4:68602/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCCTCAT‑GACCGGGACCCGGACGGGGGTAAACGTTT‑AACTGCCCCAATCACTGACAATCACAATAACATGATATAAAAAAAGGAGTGACTATTTTAAAGaa                                              >  4:704796/1‑139 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATAAT‑GAACGGGCCCCGGCCGGGGGAAAACGTTT‑CAGTGCCCCAATAACTGACATTAACAATAAACTGATATTCATAACGGAGTGACTATGTTTCAGAt                                              >  7:404056/1‑140 (MQ=255)
                                                                                          aCCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCCGTCATAAA‑GACCGGGAACCGGCCGGGGGAAAACGTTT‑AAGTGCCACATTAAAGGACATTCACAATAAAAAGATATTAATAAACGAGTGACTATGTTACAGaa                                              >  1:1202842/1‑139 (MQ=255)
                                                                                             ttCTGATAATTGTGATTTGCCGCTCTGTGGCCCCGTTAATAAT‑GACGGGTACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGt                                           <  4:753480/140‑1 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGTGCCCGTCCACAA‑GACCCGGGCCCGGACGGGGGTAAAAGTTT‑CATTGCCACAATAACTGTCAATCACAATAAAATTATATTACTAACGGCGTGTCTATGTTAAAGATAGtg                                          >  5:611980/1‑139 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGCCCCGGCCGGGGGCAAACCGTT‑CAGTGCCCCATTCACAGACATTCACAAAAACATGATATTCATCACGGAGTGACTATTTTACAGATAGta                                          >  7:1148634/1‑139 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTc                                          >  8:910819/1‑140 (MQ=255)
                                                                                              tCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGCTCATAAA‑GCCCGGGACCCGGTCGGGGGATAACGGTT‑AAGTGCCACATTAAAGGCCATTTACAATAACATTATATTCATCTCGGATTTACAATGATACAGATATTc                                          >  4:58469/1‑140 (MQ=255)
                                                                                                      ttCTGCATTGCCTCTCTGTGGTCCCGGTCATAAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTAACTGACATTAACAATAACATGATATTAATCACGGAGTGACAATGTTAAAGATAGTCGGCGCGCt                                  >  6:646984/1‑140 (MQ=255)
                                                                                                       tCTGCATTGCCTCTCTGTGGTCCCGGGGCTCAT‑TACCGGGGCCCGGGCGGGGGGAAACGGTT‑GAGGGCCCCATTAACAGAAAATAACAATAACAATATATTAATAACCGAGTGACTATTTTTCAGATATTAGgggcgcgg                                 >  2:1158028/1‑138 (MQ=255)
                                                                                                       tCTGCATTGCCTCTCTGTGGTCCCCGTCATCAT‑GACCGGGAACCGGGCGGGGGGAAACGTTT‑CCGTGCCACATTAACTGAAATTAACAATAACATGATATTAATAACAGAGTGACTATTGTTCAGATAGTGGgggcgcgg                                 >  6:805075/1‑138 (MQ=255)
                                                                                                         tGCATTGCCTCTCTGTGGTCCCGGTCAACAT‑GCCCCGGACCCGGACCGGGGAAAACGTTT‑AATTGCCACATTCACTGAAAATCAAAATAACATTATATTCATCACGGAGTTACTATGTTTCACATATTGGGCGCCCAGAt                               >  2:846206/1‑140 (MQ=255)
                                                                                                               gccccgctGTGGCCCCGGCCATCTT‑GCCGGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTctgct                         <  7:810009/134‑1 (MQ=255)
                                                                                                                ccTCTCTGTGGTCCCGGGGATCAT‑GACCGGGACCCGGCCGGGGGGAAACGTTT‑AAGTGCCACATTAACTGACATTCACAATAACATGATATTAATAACGGAGTGACTATGTTAAAGATAGTCGGCGCGCTGGTTctgctg                        >  6:861122/1‑140 (MQ=255)
                                                                                                                     ctGTGGTCCCGGTCATCAT‑GACCGGGCCCCGGCCGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGc                   >  8:1102597/1‑140 (MQ=255)
                                                                                                                     ctGTGGTCCCGGGCATCAT‑GACCGGGACCCGGACGGGGGAAAAAGTTT‑AAGTGCCACCTTCACTGACCATAACAATAAAAAGATATTCATAACGGAGTGACTATGTTTAAGATAGTCGGCGCGCTGATTCTGCTGATCGc                   >  8:216573/1‑140 (MQ=255)
                                                                                                                          gTCCCGGTCATCAT‑GACCGGGAACCGGACGGGGGAAAACGTTT‑CAGTGCCACATTAACTGACATTCACAATAAAATGATATTCATAACGGAGTGCCTATGTTACAGATAGTCGGCGCGCTGATTCTTCTGATAGCAGGAt              >  4:918419/1‑140 (MQ=255)
                                                                                                                                      tt‑GACGGGGCCCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCAttcttc  <  2:846850/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
AATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCATCAACGGCAGCAGCGCTGAAGGTTATAACAGTCAGGCCACACTGAATGTGACCTTCTGATAATTCTGCATTGCCTCTCTGTGGTCCCGGTCATCAT‑GACCGGGACCCGGACGGGGGAAAACGTTT‑CAGTGCCACATTCACTGACATTCACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATTCTGCTGATCGCAGGATTTGCCATTCTTC  >  NZ_CP022686/4677904‑4678177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A