Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I210 R1
|
226 |
19.6 |
1130256 |
95.4% |
1078264 |
84.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
3,901,121 |
A→G |
D202D (GAT→GAC) |
pstB ← |
phosphate ABC transporter ATP‑binding protein PstB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 3,901,121 | 0 | A | G | 100.0%
| 66.8
/ NA
| 21 | D202D (GAT→GAC) | pstB | phosphate ABC transporter ATP‑binding protein PstB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/12); total (9/12) |
ATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAG > NZ_CP009273/3901035‑3901204
|
atcaatTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTcc > 2:381231/1‑90 (MQ=255)
ttCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCtt > 1:29835/1‑90 (MQ=255)
tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc < 2:268087/90‑1 (MQ=255)
cAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTc < 2:497813/90‑1 (MQ=255)
aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc < 2:492766/90‑1 (MQ=255)
aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc > 1:258384/1‑90 (MQ=255)
gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa > 1:357235/1‑90 (MQ=255)
ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCCCCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc < 1:150434/90‑1 (MQ=255)
ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc < 2:223965/90‑1 (MQ=255)
ctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAg < 1:44853/90‑1 (MQ=255)
ttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc < 1:471688/65‑1 (MQ=255)
ttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc > 2:471688/1‑65 (MQ=255)
gtgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAaca > 2:464776/1‑90 (MQ=255)
gggTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGa > 1:432500/1‑78 (MQ=255)
gggTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGa < 2:432500/78‑1 (MQ=255)
gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa < 2:457867/61‑1 (MQ=255)
gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa > 1:457867/1‑61 (MQ=255)
ccacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagca < 2:357235/90‑1 (MQ=255)
ggTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcag < 2:29222/90‑1 (MQ=255)
gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa > 2:371291/1‑48 (MQ=255)
gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa < 1:371291/48‑1 (MQ=255)
|
ATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAG > NZ_CP009273/3901035‑3901204
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGCGAATG > NZ_CP009273/3901025‑3901220
|
tacactaagatggtcgtcggcagcgtcagatgtgtataagagacaGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC < SRR3722092.230941/55‑1 (MQ=60)
gagatctaccctaagatggtcgtcggcagcgtcagatgtgtataagagacagCGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC < SRR3722092.288054/48‑1 (MQ=60)
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC < SRR3722092.382304/100‑1 (MQ=60)
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC < SRR3722092.199809/100‑1 (MQ=60)
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGAAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC < SRR3722092.29184/100‑1 (MQ=60)
ACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTT > SRR3722092.30352/1‑100 (MQ=60)
CAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGC > SRR3722092.262611/1‑100 (MQ=60)
CATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCA > SRR3722092.363792/1‑100 (MQ=60)
gtgtataagagacagCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA < SRR3722092.481048/85‑1 (MQ=60)
GGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCCCCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA < SRR3722092.152965/100‑1 (MQ=60)
cgtcggcagcgtcagatgtgtataagagacagGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGT < SRR3722092.378285/68‑1 (MQ=60)
CTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACAC < SRR3722092.45617/100‑1 (MQ=60)
TGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACT > SRR3722092.440932/1‑100 (MQ=60)
TGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCtgtctcttatacacatct > SRR3722092.466911/1‑82 (MQ=60)
CCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACctgtctcttatacacatctgacg > SRR3722092.247964/1‑77 (MQ=60)
CCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTC > SRR3722092.502236/1‑100 (MQ=60)
GTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGCGAATG > SRR3722092.160877/1‑100 (MQ=60)
GTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGCGAATG > SRR3722092.1406/1‑100 (MQ=60)
|
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGCGAATG > NZ_CP009273/3901025‑3901220
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
