Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F2 I228 R1 214 26.5 1448192 97.1% 1406194 86.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,901,121 A→G D202D (GAT→GAC pstB ← phosphate ABC transporter ATP‑binding protein PstB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,901,1210AG96.8% 96.4 / ‑3.7 31D202D (GAT→GACpstBphosphate ABC transporter ATP‑binding protein PstB
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (17/13);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGC  >  NZ_CP009273/3901041‑3901205
                                                                                |                                                                                    
tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             <  1:424823/90‑1 (MQ=255)
  gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTcgg                                                                           >  2:239860/1‑88 (MQ=255)
               aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGc                                                              <  2:367808/90‑1 (MQ=255)
                 cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                                            >  1:378373/1‑90 (MQ=255)
                 cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                                            >  2:116131/1‑90 (MQ=255)
                 cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                                            >  1:71588/1‑90 (MQ=255)
                  gCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct                                                           >  1:188663/1‑90 (MQ=255)
                     gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                        >  1:108313/1‑90 (MQ=255)
                       gtgGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAAt                                                      >  2:531315/1‑90 (MQ=255)
                       gtgGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAAt                                                      >  2:397387/1‑90 (MQ=255)
                             ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCGGTTCGGTGATCAGCTCTTCAATACGccc                                                <  1:239860/90‑1 (MQ=255)
                             ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  2:36494/90‑1 (MQ=255)
                             ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  1:480938/90‑1 (MQ=255)
                             ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  1:720108/90‑1 (MQ=255)
                             ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  1:73290/90‑1 (MQ=255)
                                   acGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCt                                                                                  <  2:85556/50‑1 (MQ=255)
                                   acGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCt                                                                                  >  1:85556/1‑50 (MQ=255)
                                        cAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAg                                     >  2:498765/1‑90 (MQ=255)
                                               ctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAg                              <  2:309184/90‑1 (MQ=255)
                                                   aTGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGcc                          <  1:127511/90‑1 (MQ=255)
                                                      ttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGaa                       >  1:695783/1‑90 (MQ=255)
                                                           ggTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACgg                  >  2:539680/1‑90 (MQ=255)
                                                             tGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGtt                >  1:348776/1‑90 (MQ=255)
                                                                cGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtcgt             <  1:271662/90‑1 (MQ=255)
                                                                   tCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGa          <  1:33324/90‑1 (MQ=255)
                                                                    caccacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  1:679146/51‑1 (MQ=255)
                                                                    caccacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                >  2:679146/1‑51 (MQ=255)
                                                                     accacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagc        >  1:281024/1‑90 (MQ=255)
                                                                         cGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagca    >  2:499619/1‑90 (MQ=255)
                                                                           gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTTGAGCGCCGAACACGGTTCGTCGagcagcagc  <  2:549037/90‑1 (MQ=255)
                                                                           gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcagc  >  2:123186/1‑90 (MQ=255)
                                                                                |                                                                                    
TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGC  >  NZ_CP009273/3901041‑3901205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC  >  NZ_CP009273/3901023‑3901215
                                                                                                  |                                                                                              
TTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGT                                                                                               <  SRR3722112.713774/100‑1 (MQ=60)
TTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGT                                                                                               >  SRR3722112.366874/1‑100 (MQ=60)
  GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC                                                                                             <  SRR3722112.436254/100‑1 (MQ=60)
  GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC                                                                                             <  SRR3722112.365105/100‑1 (MQ=60)
                  TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGA                                                                             <  SRR3722112.430500/100‑1 (MQ=60)
                         GGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTC                                                                      >  SRR3722112.383284/1‑100 (MQ=60)
                         GGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTC                                                                      >  SRR3722112.72461/1‑100 (MQ=60)
                          GTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCT                                                                     >  SRR3722112.190976/1‑100 (MQ=60)
                             CATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCA                                                                  >  SRR3722112.109662/1‑100 (MQ=60)
                                          agatctacacactgacattcgtcggcagcgtcagatgtgtataagagacaGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAG                                                     <  SRR3722112.605854/50‑1 (MQ=60)
                                           GGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCctgtctcttatacacatctgacgctgccga                                                    >  SRR3722112.86617/1‑70 (MQ=60)
                                               cggcagcgtcagatgtgtataagagacagGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA                                                <  SRR3722112.689764/71‑1 (MQ=60)
                                               GGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCGGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA                                                <  SRR3722112.242709/100‑1 (MQ=60)
                                               GGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA                                                <  SRR3722112.731354/100‑1 (MQ=60)
                                               GGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA                                                <  SRR3722112.74182/100‑1 (MQ=60)
                                               GGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA                                                <  SRR3722112.487826/100‑1 (MQ=60)
                                                              CTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAA                                 >  SRR3722112.706646/1‑100 (MQ=60)
                                                                     ATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTT                          <  SRR3722112.129092/100‑1 (MQ=60)
                                                                     ATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTT                          >  SRR3722112.353156/1‑100 (MQ=60)
                                                                             GGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGC                  >  SRR3722112.284287/1‑100 (MQ=60)
                                                                                  CGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAG             <  SRR3722112.274839/100‑1 (MQ=60)
                                                                                     TCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCAC          <  SRR3722112.33744/100‑1 (MQ=60)
                                                                                            GGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGG   >  SRR3722112.388483/1‑100 (MQ=60)
                                                                                             GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC  >  SRR3722112.4149/1‑100 (MQ=60)
                                                                                             GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC  >  SRR3722112.413643/1‑100 (MQ=60)
                                                                                                  |                                                                                              
TTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC  >  NZ_CP009273/3901023‑3901215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: