Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I208 R1
|
222 |
13.1 |
731276 |
96.6% |
706412 |
85.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
3,901,121 |
A→G |
D202D (GAT→GAC) |
pstB ← |
phosphate ABC transporter ATP‑binding protein PstB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 3,901,121 | 0 | A | G | 100.0%
| 51.3
/ NA
| 15 | D202D (GAT→GAC) | pstB | phosphate ABC transporter ATP‑binding protein PstB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/9); total (6/9) |
TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGC > NZ_CP009273/3901041‑3901205
|
tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc < 2:325734/90‑1 (MQ=255)
gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg < 1:86178/90‑1 (MQ=255)
gCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct > 1:126769/1‑90 (MQ=255)
gCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct < 2:52088/90‑1 (MQ=255)
gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc < 1:222446/69‑1 (MQ=255)
gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc > 2:222446/1‑69 (MQ=255)
ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc < 1:143012/90‑1 (MQ=255)
cAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa > 1:76758/1‑83 (MQ=255)
cAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa < 2:76758/83‑1 (MQ=255)
ctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAg < 1:43333/90‑1 (MQ=255)
gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtc > 1:311566/1‑90 (MQ=255)
caccacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGag > 1:193852/1‑90 (MQ=255)
ccacGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagca < 1:217590/90‑1 (MQ=255)
ggTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcag > 1:138043/1‑90 (MQ=255)
gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcagc < 2:138043/90‑1 (MQ=255)
|
TCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGC > NZ_CP009273/3901041‑3901205
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC > NZ_CP009273/3901025‑3901215
|
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCC < SRR3722090.207397/100‑1 (MQ=60)
agatgtgtataagagaCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTT < SRR3722090.33918/84‑1 (MQ=60)
ataagagacagCATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGA < SRR3722090.224965/89‑1 (MQ=60)
GCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATC < SRR3722090.87126/100‑1 (MQ=60)
GTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCT > SRR3722090.128105/1‑100 (MQ=60)
GGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATA < SRR3722090.144500/100‑1 (MQ=60)
GAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAG > SRR3722090.77606/1‑100 (MQ=60)
CTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACAC < SRR3722090.43778/100‑1 (MQ=60)
TGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTC > SRR3722090.315569/1‑100 (MQ=60)
GGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAG > SRR3722090.195975/1‑100 (MQ=60)
CGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAG > SRR3722090.139489/1‑100 (MQ=60)
CCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTC < SRR3722090.220029/100‑1 (MQ=60)
GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCtgtctcttatacacatctccgagcccacgagact > SRR3722090.108818/1‑66 (MQ=60)
GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTctgtctcttatacacatctccgagc > SRR3722090.123606/1‑75 (MQ=60)
GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC > SRR3722090.271754/1‑100 (MQ=60)
GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC > SRR3722090.359184/1‑100 (MQ=60)
GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC > SRR3722090.165700/1‑100 (MQ=60)
GTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC > SRR3722090.116368/1‑100 (MQ=60)
|
GCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGCACTTCCGGGC > NZ_CP009273/3901025‑3901215
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
