Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,554 G→A 88.1% intergenic (‑103/+119) ygeK ← / ← ygeN pseudogene; putative 2‑component transcriptional regulator/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,5540GA88.1% 585.2 / 61.5 202intergenic (‑103/+119)ygeK/ygeNpseudogene; putative 2‑component transcriptional regulator/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (10/14);  new base A (90/88);  total (100/102)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAA  >  CP009273/2988409‑2988698
                                                                                                                                                 |                                                                                                                                                
tAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATaaa                                                                                                                                                >  2:1693179/1‑148 (MQ=255)
 aaaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATaaaa                                                                                                                                               >  1:1299175/1‑148 (MQ=255)
  aaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATACTAATAAAat                                                                                                                                              >  2:2491247/1‑148 (MQ=255)
  aaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAat                                                                                                                                              <  2:2022541/148‑1 (MQ=255)
   aCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAata                                                                                                                                             >  2:3239665/1‑148 (MQ=255)
   aCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAata                                                                                                                                             >  1:419540/1‑148 (MQ=255)
     ggCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAAtata                                                                                                                                           <  2:3019867/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  1:219565/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCACTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  1:402706/148‑1 (MQ=255)
           tgATCTGAAAATACAATTTTAATTTTTCCCATAAATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaa                                                                                                                                     <  2:1823282/148‑1 (MQ=255)
              tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaat                                                                                                                                  >  2:3224766/1‑148 (MQ=255)
                 gAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaa                                                                                                                               >  2:1046307/1‑148 (MQ=255)
                    aCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtt                                                                                                                            >  1:2701402/1‑148 (MQ=255)
                     cTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAttt                                                                                                                           >  1:750503/1‑148 (MQ=255)
                        cAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTAATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                        >  1:635661/1‑148 (MQ=255)
                            tttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTa                                                                                                                    >  2:2968557/1‑148 (MQ=255)
                                 tttttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaaa                                                                                                               <  1:2726825/148‑1 (MQ=255)
                                  ttttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaaaa                                                                                                              >  1:2965905/1‑148 (MQ=255)
                                  ttttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaaaa                                                                                                              >  1:1318667/1‑148 (MQ=255)
                                   tttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAt                                                                                                             <  2:1813754/148‑1 (MQ=255)
                                       ccATATATTCATCCTGAGTGTAAATGCTTTAACGCATTAATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACca                                                                                                         >  1:2398848/1‑148 (MQ=255)
                                        cATATAGTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACcac                                                                                                        <  1:1983641/148‑1 (MQ=255)
                                           atatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAgg                                                                                                     <  1:1140561/148‑1 (MQ=255)
                                              ttCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAt                                                                                                  >  1:2991401/1‑148 (MQ=255)
                                               tCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaaa                                                                                                               <  2:3170756/134‑1 (MQ=255)
                                               tCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAtt                                                                                                 >  1:686989/1‑148 (MQ=255)
                                                 aTCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaa                                                                                               <  1:980667/148‑1 (MQ=255)
                                                   ccTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaaaa                                                                                             >  1:2623756/1‑148 (MQ=255)
                                                      gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAt                                                                                          >  2:2292657/1‑148 (MQ=255)
                                                         tgtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAt                                                                                       >  1:3183461/1‑148 (MQ=255)
                                                          gtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATg                                                                                      <  1:860288/148‑1 (MQ=255)
                                                            aaaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgag                                                                                    <  2:1967709/148‑1 (MQ=255)
                                                            aaaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgag                                                                                    <  2:1111315/148‑1 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGNAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                   <  1:1683262/148‑1 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                   >  1:2198545/1‑148 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                   <  1:2863861/148‑1 (MQ=255)
                                                              ttgatttAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGAAAAATCAAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgat                                                                                  <  1:3080845/144‑1 (MQ=255)
                                                               tGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTCTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatg                                                                                 <  2:1716977/148‑1 (MQ=255)
                                                               tGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatg                                                                                 <  2:1059716/148‑1 (MQ=255)
                                                                gCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatga                                                                                >  1:2317834/1‑148 (MQ=255)
                                                                 cTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatgat                                                                               >  2:1852860/1‑148 (MQ=255)
                                                                   ttAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTa                                                                             <  1:37599/148‑1 (MQ=255)
                                                                       cGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAatat                                                                         >  2:2970482/1‑148 (MQ=255)
                                                                       cGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAat                                                                         >  2:1488761/1‑148 (MQ=255)
                                                                       cGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATAAAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAat                                                                         >  2:2711437/1‑148 (MQ=255)
                                                                         cattcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAatat                                                                       >  1:660593/1‑148 (MQ=255)
                                                                          attcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                      >  1:57125/1‑148 (MQ=255)
                                                                           ttcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTa                                                                     >  2:2006898/1‑148 (MQ=255)
                                                                             cattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACa                                                                   <  2:1886943/148‑1 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 <  2:2053135/148‑1 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 <  2:388200/148‑1 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 <  2:1299175/148‑1 (MQ=255)
                                                                                ttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAtt                                                                >  2:1937137/1‑148 (MQ=255)
                                                                                ttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAtt                                                                >  2:259501/1‑148 (MQ=255)
                                                                                   taATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGa                                                             >  1:1527752/1‑148 (MQ=255)
                                                                                    aaTCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGAt                                                            <  1:1693179/148‑1 (MQ=255)
                                                                                     atcatcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGAtt                                                           <  1:1536292/148‑1 (MQ=255)
                                                                                      tcatcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATta                                                          <  1:2364541/148‑1 (MQ=255)
                                                                                        atgccTTCAATTTTTATTATTATCAAAATCCAAAGAATACAATTATGAAATAATAATAAAATATAATTAAAAATATAATTTTTGCGTAAAAAAAGACCAGAGGCTTTAAAAAATCATGAGATGTTTAAAATATTACAATTAGATtata                                                        <  1:2542668/145‑1 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                        <  1:2144804/148‑1 (MQ=255)
                                                                                            cTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCa                                                    <  1:1046635/148‑1 (MQ=255)
                                                                                                cATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATc                                                <  1:2707137/148‑1 (MQ=255)
                                                                                                cATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATc                                                <  1:1666142/148‑1 (MQ=255)
                                                                                                 attattattATAAGCAAAATCCAAAGAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCa                                               <  1:2348809/148‑1 (MQ=255)
                                                                                                  ttattattaTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAt                                              <  2:1412404/148‑1 (MQ=255)
                                                                                                  ttattattaTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAt                                              >  1:1085729/1‑148 (MQ=255)
                                                                                                    attattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTa                                            <  1:1026308/148‑1 (MQ=255)
                                                                                                    attattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTa                                            >  1:1461889/1‑148 (MQ=255)
                                                                                                     ttattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTaa                                           <  2:2561370/148‑1 (MQ=255)
                                                                                                      tattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTaaa                                          >  1:2319511/1‑148 (MQ=255)
                                                                                                        ttatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACt                                        >  2:89464/1‑148 (MQ=255)
                                                                                                          ataAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTg                                      >  2:3288915/1‑148 (MQ=255)
                                                                                                          ataAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTg                                      <  1:1279732/148‑1 (MQ=255)
                                                                                                          ataAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTg                                      <  1:2789003/148‑1 (MQ=255)
                                                                                                            aaGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAg                                    <  2:2701402/148‑1 (MQ=255)
                                                                                                            aaGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAAATTGAg                                    >  2:2856876/1‑148 (MQ=255)
                                                                                                             aGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGc                                   <  1:187394/148‑1 (MQ=255)
                                                                                                             aGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGc                                   >  2:2754890/1‑148 (MQ=255)
                                                                                                             aGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGc                                   >  1:3304510/1‑148 (MQ=255)
                                                                                                              gCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGcc                                  <  1:534036/148‑1 (MQ=255)
                                                                                                                 aaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAgg                               >  2:1377048/1‑148 (MQ=255)
                                                                                                                    tCCAAATAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAg                            <  2:2660819/148‑1 (MQ=255)
                                                                                                                    tCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAg                            >  2:573310/1‑148 (MQ=255)
                                                                                                                    tCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAg                            <  1:2817699/148‑1 (MQ=255)
                                                                                                                     ccAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGc                           >  1:178306/1‑148 (MQ=255)
                                                                                                                     ccAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGc                           >  1:1602531/1‑148 (MQ=255)
                                                                                                                     ccAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGc                           >  2:755682/1‑148 (MQ=255)
                                                                                                                     ccAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGc                           <  1:1060648/148‑1 (MQ=255)
                                                                                                                      cAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCt                          >  2:452662/1‑148 (MQ=255)
                                                                                                                          gAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATaa                      >  2:641118/1‑148 (MQ=255)
                                                                                                                              aCATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTcc                  <  2:1431390/148‑1 (MQ=255)
                                                                                                                                 ttGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCa               >  2:3074513/1‑148 (MQ=255)
                                                                                                                                    atgaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAggg            <  2:2162542/148‑1 (MQ=255)
                                                                                                                                     tgaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGc           >  1:2208349/1‑148 (MQ=255)
                                                                                                                                      gaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGATATAAGTCCTCAGGGCt          >  1:1356110/1‑148 (MQ=255)
                                                                                                                                         ataataataAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGc       >  2:2984231/1‑148 (MQ=255)
                                                                                                                                          taataatGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGcc      >  2:696516/1‑148 (MQ=255)
                                                                                                                                             taataaAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa   >  1:2712068/1‑148 (MQ=255)
                                                                                                                                             taataaAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa   >  2:2148137/1‑148 (MQ=255)
                                                                                                                                              aatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaaa  >  2:1544912/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAA  >  CP009273/2988409‑2988698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGA  >  CP009273/2988406‑2988702
                                                                                                                                                    |                                                                                                                                                    
TCATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAA                                                                                                                                                     >  SRR19973070.3014792/1‑150 (MQ=60)
 CATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAA                                                                                                                                                    >  SRR19973070.6652/1‑150 (MQ=60)
 CATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAA                                                                                                                                                    >  SRR19973070.6822/1‑150 (MQ=60)
  ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAA                                                                                                                                                   >  SRR19973070.1308215/1‑150 (MQ=60)
  ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAA                                                                                                                                                   >  SRR19973070.1313039/1‑150 (MQ=60)
   TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAAT                                                                                                                                                  <  SRR19973070.1294665/150‑1 (MQ=60)
    AAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATA                                                                                                                                                 >  SRR19973070.422600/1‑150 (MQ=60)
          CTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTA                                                                                                                                           <  SRR19973070.221157/150‑1 (MQ=60)
          CTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCACTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTA                                                                                                                                           <  SRR19973070.405665/150‑1 (MQ=60)
           TGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAA                                                                                                                                          <  SRR19973070.2881888/150‑1 (MQ=60)
           TGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAA                                                                                                                                          <  SRR19973070.1821841/150‑1 (MQ=60)
              TGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAA                                                                                                                                       >  SRR19973070.596595/1‑150 (MQ=60)
                ATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATA                                                                                                                                     <  SRR19973070.1180776/150‑1 (MQ=60)
                   TGAAACTACAATTTTAATCTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAA                                                                                                                                  <  SRR19973070.2816494/150‑1 (MQ=60)
                     AAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATT                                                                                                                                >  SRR19973070.2718877/1‑150 (MQ=60)
                     AAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATT                                                                                                                                >  SRR19973070.2718901/1‑150 (MQ=60)
                     AAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATT                                                                                                                                <  SRR19973070.1291127/150‑1 (MQ=60)
                      AACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTT                                                                                                                               >  SRR19973070.755802/1‑150 (MQ=60)
                        CTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTT                                                                                                                             <  SRR19973070.415707/150‑1 (MQ=60)
                        CTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATATTT                                                                                                                             >  SRR19973070.3255017/1‑150 (MQ=60)
                         TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTAATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                            >  SRR19973070.2312045/1‑150 (MQ=60)
                         TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTAATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                            >  SRR19973070.640181/1‑150 (MQ=60)
                           CAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCG                                                                                                                          <  SRR19973070.1777524/150‑1 (MQ=60)
                              TTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAA                                                                                                                       <  SRR19973070.1859310/150‑1 (MQ=60)
                                   ATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAA                                                                                                                  >  SRR19973070.1327791/1‑150 (MQ=60)
                                   ATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAA                                                                                                                  >  SRR19973070.2984969/1‑150 (MQ=60)
                                    TTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAT                                                                                                                 <  SRR19973070.2744413/150‑1 (MQ=60)
                                        TCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTAATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCA                                                                                                             >  SRR19973070.2414678/1‑150 (MQ=60)
                                         CCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCAC                                                                                                            <  SRR19973070.2402762/150‑1 (MQ=60)
                                         CCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCAC                                                                                                            <  SRR19973070.732654/150‑1 (MQ=60)
                                         CCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGATTAAAAAAATACCAA                                                                                                            >  SRR19973070.348516/1‑150 (MQ=60)
                                           CATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAG                                                                                                          <  SRR19973070.2051094/150‑1 (MQ=60)
                                           CATATAGTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAG                                                                                                          <  SRR19973070.1997070/150‑1 (MQ=60)
                                             TATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGC                                                                                                        >  SRR19973070.1062641/1‑150 (MQ=60)
                                              ATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCA                                                                                                       <  SRR19973070.1148473/150‑1 (MQ=60)
                                               TATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAT                                                                                                      >  SRR19973070.3010563/1‑150 (MQ=60)
                                                ATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAgatcggaagag                                                                                                     >  SRR19973070.3191280/1‑139 (MQ=60)
                                                ATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATT                                                                                                     >  SRR19973070.691892/1‑150 (MQ=60)
                                                    ATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAA                                                                                                 >  SRR19973070.2640876/1‑150 (MQ=60)
                                                    ATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAA                                                                                                 >  SRR19973070.275166/1‑150 (MQ=60)
                                                    ATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAA                                                                                                 <  SRR19973070.987663/150‑1 (MQ=60)
                                                       CTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAT                                                                                              <  SRR19973070.847362/150‑1 (MQ=60)
                                                          AGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAT                                                                                           >  SRR19973070.3204060/1‑150 (MQ=60)
                                                             GTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAG                                                                                        <  SRR19973070.866257/150‑1 (MQ=60)
                                                              TAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGA                                                                                       <  SRR19973070.269974/150‑1 (MQ=60)
                                                              TAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGA                                                                                       >  SRR19973070.2211725/1‑150 (MQ=60)
                                                              TAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGA                                                                                       >  SRR19973070.2213044/1‑150 (MQ=60)
                                                               AAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGAT                                                                                      <  SRR19973070.1922281/150‑1 (MQ=60)
                                                                AATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGNAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATG                                                                                     <  SRR19973070.1694804/150‑1 (MQ=60)
                                                                AATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGNAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATG                                                                                     <  SRR19973070.1694715/150‑1 (MQ=60)
                                                                AATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATG                                                                                     <  SRR19973070.2882417/150‑1 (MQ=60)
                                                                 ttgaTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGAAAAATCAAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGA                                                                                    <  SRR19973070.3100643/146‑1 (MQ=60)
                                                                 ATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGA                                                                                    >  SRR19973070.2333234/1‑150 (MQ=60)
                                                                 ATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGA                                                                                    >  SRR19973070.2333890/1‑150 (MQ=60)
                                                                 ATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGA                                                                                    <  SRR19973070.1080561/150‑1 (MQ=60)
                                                                    CTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTA                                                                                 >  SRR19973070.443510/1‑150 (MQ=60)
                                                                      TTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAA                                                                               <  SRR19973070.38005/150‑1 (MQ=60)
                                                                      TTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAA                                                                               <  SRR19973070.38080/150‑1 (MQ=60)
                                                                      TTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAA                                                                               <  SRR19973070.1701857/150‑1 (MQ=60)
                                                                        AACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAAT                                                                             <  SRR19973070.2379052/150‑1 (MQ=60)
                                                                          CGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAT                                                                           >  SRR19973070.665296/1‑150 (MQ=60)
                                                                           GCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATT                                                                          >  SRR19973070.57786/1‑150 (MQ=60)
                                                                                 ATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATT                                                                    >  SRR19973070.2176909/1‑150 (MQ=60)
                                                                                 ATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATAAAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATT                                                                    <  SRR19973070.2728962/150‑1 (MQ=60)
                                                                                 ATTTATAATCATACCTTCCATTATTATTATAAGCAAAATACAAAGAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCTTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATT                                                                    <  SRR19973070.2728990/150‑1 (MQ=60)
                                                                                    TATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGA                                                                 >  SRR19973070.2556079/1‑150 (MQ=60)
                                                                                    TATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGA                                                                 >  SRR19973070.1538485/1‑150 (MQ=60)
                                                                                       AATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTA                                                              <  SRR19973070.1704823/150‑1 (MQ=60)
                                                                                       AATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTA                                                              >  SRR19973070.2999189/1‑150 (MQ=60)
                                                                                        ATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTAT                                                             <  SRR19973070.1546631/150‑1 (MQ=60)
                                                                                        ATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTAT                                                             <  SRR19973070.1547086/150‑1 (MQ=60)
                                                                                         TCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATA                                                            <  SRR19973070.2380184/150‑1 (MQ=60)
                                                                                         TCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATA                                                            <  SRR19973070.1597743/150‑1 (MQ=60)
                                                                                          CATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATAT                                                           >  SRR19973070.1110810/1‑150 (MQ=60)
                                                                                           ATGCCTTCAATTTTTATTATTATCAAAATCCAAAGAATACAATTATGAAATAATAATAAAATATAATTAAAAATATAATTTTTGCGTAAAAAAAGACCAGAGGCTTTAAAAAATCATGAGATGTTTAAAATATTACAATTAGATTATATT                                                          <  SRR19973070.2559297/150‑1 (MQ=60)
                                                                                           ATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATT                                                          <  SRR19973070.2159099/150‑1 (MQ=60)
                                                                                            TCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTC                                                         >  SRR19973070.38340/1‑150 (MQ=60)
                                                                                              CCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAT                                                       <  SRR19973070.2738651/150‑1 (MQ=60)
                                                                                               CTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAA                                                      <  SRR19973070.1053677/150‑1 (MQ=60)
                                                                                               CTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAA                                                      <  SRR19973070.1054009/150‑1 (MQ=60)
                                                                                                   CATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAT                                                  >  SRR19973070.1093259/1‑150 (MQ=60)
                                                                                                   CATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAT                                                  <  SRR19973070.2724640/150‑1 (MQ=60)
                                                                                                   CATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAT                                                  <  SRR19973070.1677589/150‑1 (MQ=60)
                                                                                                    ATTATTATTATAAGCAAAATCCAAAGAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                 <  SRR19973070.2364386/150‑1 (MQ=60)
                                                                                                    ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                 <  SRR19973070.693868/150‑1 (MQ=60)
                                                                                                    ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                 <  SRR19973070.3052424/150‑1 (MQ=60)
                                                                                                    ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                 <  SRR19973070.3052428/150‑1 (MQ=60)
                                                                                                    ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                 <  SRR19973070.2364367/150‑1 (MQ=60)
                                                                                                     TTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTA                                                >  SRR19973070.1472100/1‑150 (MQ=60)
                                                                                                       ATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAA                                              <  SRR19973070.1033584/150‑1 (MQ=60)
                                                                                                       ATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAA                                              >  SRR19973070.2334927/1‑150 (MQ=60)
                                                                                                         TATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACT                                            >  SRR19973070.1348190/1‑150 (MQ=60)
                                                                                                             ATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAG                                        <  SRR19973070.2806966/150‑1 (MQ=60)
                                                                                                             ATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAG                                        <  SRR19973070.1288674/150‑1 (MQ=60)
                                                                                                              TAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGC                                       >  SRR19973070.3325666/1‑150 (MQ=60)
                                                                                                                AGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCA                                     <  SRR19973070.188851/150‑1 (MQ=60)
                                                                                                                 GCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAG                                    <  SRR19973070.537626/150‑1 (MQ=60)
                                                                                                                 GCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAG                                    <  SRR19973070.537646/150‑1 (MQ=60)
                                                                                                                  CAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGG                                   >  SRR19973070.2918702/1‑150 (MQ=60)
                                                                                                                   AAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGG                                  <  SRR19973070.1567722/150‑1 (MQ=60)
                                                                                                                   AAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGG                                  >  SRR19973070.2255566/1‑150 (MQ=60)
                                                                                                                     AATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAG                                <  SRR19973070.880997/150‑1 (MQ=60)
                                                                                                                      ATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGC                               >  SRR19973070.179720/1‑150 (MQ=60)
                                                                                                                      ATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGC                               >  SRR19973070.1613717/1‑150 (MQ=60)
                                                                                                                       TCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCT                              <  SRR19973070.2835912/150‑1 (MQ=60)
                                                                                                                       TCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCT                              >  SRR19973070.440800/1‑150 (MQ=60)
                                                                                                                        CCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTA                             <  SRR19973070.1068087/150‑1 (MQ=60)
                                                                                                                                      GATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGC               >  SRR19973070.1641250/1‑150 (MQ=60)
                                                                                                                                      GATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGC               >  SRR19973070.2219307/1‑150 (MQ=60)
                                                                                                                                      GATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGC               >  SRR19973070.2222882/1‑150 (MQ=60)
                                                                                                                                       ATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCT              >  SRR19973070.1467922/1‑150 (MQ=60)
                                                                                                                                       ATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCT              >  SRR19973070.2754616/1‑150 (MQ=60)
                                                                                                                                       ATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGATATAAGTCCTCAGGGCT              >  SRR19973070.1365378/1‑150 (MQ=60)
                                                                                                                                        AGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCCTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTT             <  SRR19973070.2563688/150‑1 (MQ=60)
                                                                                                                                          AAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGC           >  SRR19973070.15004/1‑150 (MQ=60)
                                                                                                                                              AATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAA       >  SRR19973070.2729595/1‑150 (MQ=60)
                                                                                                                                                TAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAAC     <  SRR19973070.2507415/150‑1 (MQ=60)
                                                                                                                                                  ATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG   <  SRR19973070.645690/150‑1 (MQ=60)
                                                                                                                                                   TGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGA  >  SRR19973070.2463527/1‑150 (MQ=60)
                                                                                                                                                   TAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGA  <  SRR19973070.1546729/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TCATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGA  >  CP009273/2988406‑2988702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑