Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,554 G→A 100% intergenic (‑103/+119) ygeK ← / ← ygeN pseudogene; putative 2‑component transcriptional regulator/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,5540GA97.9% 459.1 / 0.6 140intergenic (‑103/+119)ygeK/ygeNpseudogene; putative 2‑component transcriptional regulator/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base A (65/72);  total (68/72)
Fisher's exact test for biased strand distribution p-value = 1.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 150 total aligned reads displayed.

TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAA  >  CP009273/2988409‑2988697
                                                                                                                                                 |                                                                                                                                               
tAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATaaa                                                                                                                                               >  2:1866850/1‑148 (MQ=255)
  aaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAat                                                                                                                                             <  1:3497886/148‑1 (MQ=255)
   aCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAata                                                                                                                                            <  1:2641453/148‑1 (MQ=255)
   aCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAAAACATTGATGAAATAATAATAAAata                                                                                                                                            >  2:3468306/1‑148 (MQ=255)
    cGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAatat                                                                                                                                           <  2:3262861/148‑1 (MQ=255)
    cGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAatat                                                                                                                                           <  2:1453502/148‑1 (MQ=255)
      gctgctGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATaa                                                                                                                                         <  2:1519394/148‑1 (MQ=255)
             aTCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaaa                                                                                                                                  <  1:1138505/148‑1 (MQ=255)
             aTCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaaa                                                                                                                                  <  1:506890/148‑1 (MQ=255)
             aTCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaaa                                                                                                                                  >  2:730500/1‑148 (MQ=255)
                 gAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaa                                                                                                                              <  1:1763152/148‑1 (MQ=255)
                   aaCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaat                                                                                                                            >  2:2287097/1‑148 (MQ=255)
                   aaCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaat                                                                                                                            <  1:1651953/148‑1 (MQ=255)
                   aaCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaat                                                                                                                            <  1:228230/148‑1 (MQ=255)
                    aCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtt                                                                                                                           >  2:929866/1‑148 (MQ=255)
                    aCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtt                                                                                                                           >  2:2997671/1‑148 (MQ=255)
                     cTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAttt                                                                                                                          <  1:3467893/148‑1 (MQ=255)
                     cTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAttt                                                                                                                          >  2:2812105/1‑148 (MQ=255)
                     cTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAttt                                                                                                                          >  2:2812152/1‑148 (MQ=255)
                         aattttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGc                                                                                                                      >  1:3262653/1‑148 (MQ=255)
                         aattttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGc                                                                                                                      >  1:297648/1‑148 (MQ=255)
                          attttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCg                                                                                                                     <  1:946229/148‑1 (MQ=255)
                           ttttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGt                                                                                                                    >  1:2810115/1‑148 (MQ=255)
                                   tttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAt                                                                                                            >  1:721219/1‑148 (MQ=255)
                                   tttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAt                                                                                                            <  2:1190983/148‑1 (MQ=255)
                                     tCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAc                                                                                                          >  2:579286/1‑148 (MQ=255)
                                      cccATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAcc                                                                                                         >  2:475473/1‑148 (MQ=255)
                                           atatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAgg                                                                                                    >  2:186866/1‑148 (MQ=255)
                                           atatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAgg                                                                                                    >  2:177929/1‑148 (MQ=255)
                                           atatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAgg                                                                                                    <  2:2131702/148‑1 (MQ=255)
                                            tatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGc                                                                                                   <  2:1184379/148‑1 (MQ=255)
                                               tCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAtt                                                                                                <  2:1596025/148‑1 (MQ=255)
                                                   ccTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaaaa                                                                                            >  1:2205595/1‑148 (MQ=255)
                                                          ttAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATg                                                                                     <  1:3105201/147‑1 (MQ=255)
                                                          gtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATg                                                                                     >  2:2395648/1‑148 (MQ=255)
                                                            aaaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgag                                                                                   >  2:357052/1‑148 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                  >  2:1395746/1‑148 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                  >  2:2457048/1‑148 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                  <  2:2161865/148‑1 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                  >  2:2196253/1‑148 (MQ=255)
                                                                     aaCGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTaaa                                                                          <  1:929866/148‑1 (MQ=255)
                                                                     aaCGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTaaa                                                                          <  1:2997671/148‑1 (MQ=255)
                                                                      aCGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTaaaa                                                                         <  1:1866850/148‑1 (MQ=255)
                                                                       cGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAat                                                                        <  2:102337/148‑1 (MQ=255)
                                                                       cGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAat                                                                        <  2:102388/148‑1 (MQ=255)
                                                                        gCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAata                                                                       <  1:2366749/148‑1 (MQ=255)
                                                                             cattTATACTCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACa                                                                  <  1:2146704/148‑1 (MQ=255)
                                                                             cattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACa                                                                  <  2:929363/148‑1 (MQ=255)
                                                                             cattTATAATCATCCCTACCATTAATATTATAAGCAAACTCCAAACAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACa                                                                  <  1:2384267/148‑1 (MQ=255)
                                                                                ttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAtt                                                               <  2:3386918/148‑1 (MQ=255)
                                                                                   taATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGa                                                            <  1:2797833/148‑1 (MQ=255)
                                                                                   taATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGa                                                            >  2:2454742/1‑148 (MQ=255)
                                                                                     atcatcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGAtt                                                          >  1:2207877/1‑148 (MQ=255)
                                                                                      tcatcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATta                                                         >  1:1565780/1‑148 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                       <  1:3288749/148‑1 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                       <  2:1997228/148‑1 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                       >  2:371460/1‑148 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                       >  1:1520753/1‑148 (MQ=255)
                                                                                          cccTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATAtt                                                     >  2:243124/1‑148 (MQ=255)
                                                                                          cccTTACATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATAtt                                                     <  2:451262/148‑1 (MQ=255)
                                                                                           ccTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTc                                                    >  2:1405101/1‑148 (MQ=255)
                                                                                             ttCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaa                                                  >  2:240776/1‑148 (MQ=255)
                                                                                             ttCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaa                                                  >  2:240380/1‑148 (MQ=255)
                                                                                             ttCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaa                                                  >  1:1593137/1‑148 (MQ=255)
                                                                                             ttCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaa                                                  >  2:588509/1‑148 (MQ=255)
                                                                                              taaattattattATAATAAAAATCCAAATAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaaa                                                 <  2:2144350/145‑1 (MQ=255)
                                                                                              tCCATTATTATTATAAGCAAAATCCAAATAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaaa                                                 <  2:353751/148‑1 (MQ=255)
                                                                                                 attattattATAAGCAAAATCCAAAGAATACATTGATGATATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCa                                              <  1:2971544/148‑1 (MQ=255)
                                                                                                  ttattattaTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAt                                             >  2:106416/1‑148 (MQ=255)
                                                                                                  ttattattaTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAt                                             <  2:1089217/148‑1 (MQ=255)
                                                                                                  ttattattaTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAt                                             <  2:1645030/148‑1 (MQ=255)
                                                                                                   tattattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAtt                                            >  1:629935/1‑148 (MQ=255)
                                                                                                     ttattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTaa                                          >  2:2654457/1‑148 (MQ=255)
                                                                                                     ttattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTaa                                          >  2:2654439/1‑148 (MQ=255)
                                                                                                     ttattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTaa                                          <  1:1456394/148‑1 (MQ=255)
                                                                                                        ttatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACt                                       >  2:1614509/1‑148 (MQ=255)
                                                                                                          ataAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTg                                     >  1:1713255/1‑148 (MQ=255)
                                                                                                            aaGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAg                                   >  2:2325368/1‑148 (MQ=255)
                                                                                                                    tCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAg                           >  2:1297339/1‑148 (MQ=255)
                                                                                                                    tCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAAATTGAGCCAGGGAg                           <  1:2607179/148‑1 (MQ=255)
                                                                                                                      cAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCt                         >  1:208783/1‑148 (MQ=255)
                                                                                                                        aaGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCtat                       >  1:1777031/1‑148 (MQ=255)
                                                                                                                         aGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCtata                      <  1:3060357/148‑1 (MQ=255)
                                                                                                                         aGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCtata                      >  2:335575/1‑148 (MQ=255)
                                                                                                                            aTACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGt                   >  2:1285756/1‑148 (MQ=255)
                                                                                                                            aTACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGt                   >  2:2591807/1‑148 (MQ=255)
                                                                                                                             tACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTc                  >  2:2036527/1‑148 (MQ=255)
                                                                                                                              aCATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTcc                 <  1:2837521/148‑1 (MQ=255)
                                                                                                                                 ttGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCa              >  1:1055908/1‑148 (MQ=255)
                                                                                                                                 ttGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCa              >  1:1055917/1‑148 (MQ=255)
                                                                                                                                     tgaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGc          <  2:721219/148‑1 (MQ=255)
                                                                                                                                       aaaTAATAATAAAATATAATTATAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAACACTCATGAGATGATTAAAATATTACACTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCtt        <  1:2287097/148‑1 (MQ=255)
                                                                                                                                        aataataatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTg       <  2:261385/148‑1 (MQ=255)
                                                                                                                                          taataataaAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGcc     >  2:356126/1‑148 (MQ=255)
                                                                                                                                           aataatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCa    >  2:3133698/1‑148 (MQ=255)
                                                                                                                                           aataatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCa    <  1:2942890/148‑1 (MQ=255)
                                                                                                                                            ataataAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaa   <  1:1339663/148‑1 (MQ=255)
                                                                                                                                             taataaAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa  <  1:693925/148‑1 (MQ=255)
                                                                                                                                             taataaAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa  >  1:2900510/1‑148 (MQ=255)
                                                                                                                                             taataaAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa  >  1:2009115/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAA  >  CP009273/2988409‑2988697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG  >  CP009273/2988407‑2988701
                                                                                                                                                   |                                                                                                                                                   
CATACACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAA                                                                                                                                                   <  SRR19973067.838371/150‑1 (MQ=60)
CATACACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAA                                                                                                                                                   <  SRR19973067.2634188/150‑1 (MQ=60)
 ttaacCGGCTGCTGATCTGAAACTACTATTTTACTTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATACCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAA                                                                                                                                                  <  SRR19973067.2929397/145‑1 (MQ=60)
 ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAA                                                                                                                                                  <  SRR19973067.2654843/150‑1 (MQ=60)
    AACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATAT                                                                                                                                               <  SRR19973067.3520070/150‑1 (MQ=60)
     ACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATA                                                                                                                                              <  SRR19973067.2658529/150‑1 (MQ=60)
     ACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATA                                                                                                                                              <  SRR19973067.2658516/150‑1 (MQ=60)
     ACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATA                                                                                                                                              <  SRR19973067.3420158/150‑1 (MQ=60)
            CTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAA                                                                                                                                       <  SRR19973067.1879527/150‑1 (MQ=60)
              GATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAAT                                                                                                                                     <  SRR19973067.1758580/150‑1 (MQ=60)
               ATCTGAAACTACACTTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATA                                                                                                                                    <  SRR19973067.2928983/150‑1 (MQ=60)
               ATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATA                                                                                                                                    <  SRR19973067.510355/150‑1 (MQ=60)
               ATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATA                                                                                                                                    <  SRR19973067.1146164/150‑1 (MQ=60)
                   GAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAT                                                                                                                                <  SRR19973067.1775146/150‑1 (MQ=60)
                     AACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTT                                                                                                                              <  SRR19973067.1663330/150‑1 (MQ=60)
                     AACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTT                                                                                                                              <  SRR19973067.229872/150‑1 (MQ=60)
                       CTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTT                                                                                                                            <  SRR19973067.3489932/150‑1 (MQ=60)
                        TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAAGGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                           <  SRR19973067.38277/150‑1 (MQ=60)
                         ACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGC                                                                                                                          >  SRR19973067.299544/1‑150 (MQ=60)
                         ACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGC                                                                                                                          >  SRR19973067.3283027/1‑150 (MQ=60)
                           AATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGT                                                                                                                        >  SRR19973067.2828227/1‑150 (MQ=60)
                            ATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTA                                                                                                                       <  SRR19973067.952692/150‑1 (MQ=60)
                                 AATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAA                                                                                                                  <  SRR19973067.2069238/150‑1 (MQ=60)
                                   TTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAT                                                                                                                >  SRR19973067.726229/1‑150 (MQ=60)
                                    TTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATA                                                                                                               >  SRR19973067.3272725/1‑150 (MQ=60)
                                        CCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCAC                                                                                                           <  SRR19973067.966700/150‑1 (MQ=60)
                                                   ATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAA                                                                                                >  SRR19973067.2220303/1‑150 (MQ=60)
                                                            TTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAG                                                                                       <  SRR19973067.3124953/150‑1 (MQ=60)
                                                                     TTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAA                                                                              <  SRR19973067.1565139/150‑1 (MQ=60)
                                                                       AACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAAT                                                                            <  SRR19973067.936154/150‑1 (MQ=60)
                                                                       AACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAAT                                                                            <  SRR19973067.3016680/150‑1 (MQ=60)
                                                                        ACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATA                                                                           <  SRR19973067.1879582/150‑1 (MQ=60)
                                                                          GCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATT                                                                         <  SRR19973067.2382157/150‑1 (MQ=60)
                                                                              TCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAA                                                                     >  SRR19973067.919065/1‑150 (MQ=60)
                                                                               CATTTATACTCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAT                                                                    <  SRR19973067.2161058/150‑1 (MQ=60)
                                                                               CATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAT                                                                    <  SRR19973067.2161290/150‑1 (MQ=60)
                                                                               CATTTATAATCATCCCTACCATTAATATTATAAGCAAACTCCAAACAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAT                                                                    <  SRR19973067.2399743/150‑1 (MQ=60)
                                                                                    ATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGAT                                                               <  SRR19973067.2323426/150‑1 (MQ=60)
                                                                                    ATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGAT                                                               >  SRR19973067.2406526/1‑150 (MQ=60)
                                                                                     TAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATT                                                              >  SRR19973067.2222599/1‑150 (MQ=60)
                                                                                     TAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATT                                                              <  SRR19973067.2815880/150‑1 (MQ=60)
                                                                                      AATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTA                                                             >  SRR19973067.1576492/1‑150 (MQ=60)
                                                                                        TCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATA                                                           >  SRR19973067.1530935/1‑150 (MQ=60)
                                                                                          ATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATT                                                         <  SRR19973067.3309409/150‑1 (MQ=60)
                                                                                             CCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAA                                                      >  SRR19973067.1604058/1‑150 (MQ=60)
                                                                                             CCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAAAAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATCAGATGATTAAAATATTAAAATAAGATTATATTCAA                                                      >  SRR19973067.1604141/1‑150 (MQ=60)
                                                                                                   ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGATATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                <  SRR19973067.2990289/150‑1 (MQ=60)
                                                                                                   ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGATATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                <  SRR19973067.2990322/150‑1 (MQ=60)
                                                                                                   ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                >  SRR19973067.634134/1‑150 (MQ=60)
                                                                                                   ATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATT                                                >  SRR19973067.635434/1‑150 (MQ=60)
                                                                                                       TTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAAC                                            <  SRR19973067.1466143/150‑1 (MQ=60)
                                                                                                          TTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTG                                         >  SRR19973067.1725003/1‑150 (MQ=60)
                                                                                                                      TCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCT                             >  SRR19973067.1119298/1‑150 (MQ=60)
                                                                                                                      TCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCT                             >  SRR19973067.210347/1‑150 (MQ=60)
                                                                                                                      TCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAAATTGAGCCAGGGAGCT                             <  SRR19973067.2624116/150‑1 (MQ=60)
                                                                                                                        CAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTAT                           >  SRR19973067.1789065/1‑150 (MQ=60)
                                                                                                                        CAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTAT                           >  SRR19973067.2831800/1‑150 (MQ=60)
                                                                                                                          AAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAA                         >  SRR19973067.3024503/1‑150 (MQ=60)
                                                                                                                           AGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAG                        <  SRR19973067.3079841/150‑1 (MQ=60)
                                                                                                                                ACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTC                   <  SRR19973067.2855750/150‑1 (MQ=60)
                                                                                                                                 CATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCA                  >  SRR19973067.1063148/1‑150 (MQ=60)
                                                                                                                                 CATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCA                  >  SRR19973067.1063157/1‑150 (MQ=60)
                                                                                                                                  ATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAG                 >  SRR19973067.2241406/1‑150 (MQ=60)
                                                                                                                                         AAATAATAATAAAATATAATTATAAATAATATTTTTGCGTAAAAAAATACCACAGGCATTAAACACTCATGAGATGATTAAAATATTACACTTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGC          <  SRR19973067.2302216/150‑1 (MQ=60)
                                                                                                                                           ATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCA        <  SRR19973067.1405224/150‑1 (MQ=60)
                                                                                                                                             AATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAA      >  SRR19973067.2022642/1‑150 (MQ=60)
                                                                                                                                             AATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAA      >  SRR19973067.2919011/1‑150 (MQ=60)
                                                                                                                                             AATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAA      <  SRR19973067.2961537/150‑1 (MQ=60)
                                                                                                                                              ATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAA     <  SRR19973067.1348827/150‑1 (MQ=60)
                                                                                                                                               TAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAAC    <  SRR19973067.698712/150‑1 (MQ=60)
                                                                                                                                                 ATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG  <  SRR19973067.3300969/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
CATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG  >  CP009273/2988407‑2988701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑