Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,554 G→A 100% intergenic (‑103/+119) ygeK ← / ← ygeN pseudogene; putative 2‑component transcriptional regulator/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,5540GA96.6% 376.9 / 3.7 117intergenic (‑103/+119)ygeK/ygeNpseudogene; putative 2‑component transcriptional regulator/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base A (54/59);  total (54/63)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 122 total aligned reads displayed.

TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAA  >  CP009273/2988409‑2988698
                                                                                                                                                 |                                                                                                                                                
tAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATaaa                                                                                                                                                <  2:1441289/148‑1 (MQ=255)
  aaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAat                                                                                                                                              <  1:1522915/148‑1 (MQ=255)
  aaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAat                                                                                                                                              <  1:1522934/148‑1 (MQ=255)
  aaCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAat                                                                                                                                              <  2:3238687/148‑1 (MQ=255)
     ggCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAAtata                                                                                                                                           <  1:3344144/148‑1 (MQ=255)
      gctgctGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATaa                                                                                                                                          <  2:2943780/148‑1 (MQ=255)
      gctgctGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATaa                                                                                                                                          <  2:2002291/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  2:968268/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  1:267944/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  1:268032/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  1:974130/148‑1 (MQ=255)
       ctgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCAAAAGAATACATTGATGAAATAATAATAAAATATAAt                                                                                                                                         <  1:268214/148‑1 (MQ=255)
         gctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTa                                                                                                                                       <  1:2740135/148‑1 (MQ=255)
            gATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaa                                                                                                                                    >  1:3434055/1‑148 (MQ=255)
            gATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaa                                                                                                                                    >  1:1696486/1‑148 (MQ=255)
            gATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaa                                                                                                                                    >  1:2277311/1‑148 (MQ=255)
             aTCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaaa                                                                                                                                   >  1:386826/1‑148 (MQ=255)
             aTCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaaa                                                                                                                                   >  1:386815/1‑148 (MQ=255)
              tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaat                                                                                                                                  >  1:552751/1‑148 (MQ=255)
              tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaat                                                                                                                                  <  1:2493118/148‑1 (MQ=255)
              tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaat                                                                                                                                  >  1:552809/1‑148 (MQ=255)
               cTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaata                                                                                                                                 <  2:1104104/148‑1 (MQ=255)
                 gAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGATTTAACTCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCAAAAGAATAAATTGATGAAATAATAATAAAATATAATTAaaaataaa                                                                                                                               >  1:1000805/1‑148 (MQ=255)
                  aaaCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaa                                                                                                                              <  1:917812/148‑1 (MQ=255)
                    aCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtt                                                                                                                            >  2:1743935/1‑148 (MQ=255)
                        cAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                        >  2:932515/1‑148 (MQ=255)
                        cAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                        >  2:932248/1‑148 (MQ=255)
                         aattttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGc                                                                                                                       <  1:2446106/148‑1 (MQ=255)
                         aattttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGc                                                                                                                       <  1:814571/148‑1 (MQ=255)
                             ttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaa                                                                                                                   >  2:2598722/1‑148 (MQ=255)
                              taattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaa                                                                                                                  >  2:1806008/1‑148 (MQ=255)
                                attttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaa                                                                                                                >  2:1135343/1‑148 (MQ=255)
                                attttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaa                                                                                                                >  2:2441129/1‑148 (MQ=255)
                                        cATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACcac                                                                                                        <  1:2364751/148‑1 (MQ=255)
                                         atatatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACcaca                                                                                                       <  2:2336419/148‑1 (MQ=255)
                                          aatatTCATCCTGAGTGTAAATGCTTTAACGCATTCATGTATAATCATCCCTTCCATTATTAATATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAATAAAAATAAAATTAATGCGTAAAAAAATACAACAg                                                                                                      >  1:2736816/2‑148 (MQ=255)
                                            tatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGc                                                                                                    <  2:3271821/148‑1 (MQ=255)
                                            tatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGc                                                                                                    >  2:2342897/1‑148 (MQ=255)
                                            tatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGc                                                                                                    <  2:1531714/148‑1 (MQ=255)
                                              ttCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAACAGGCAt                                                                                                  <  1:216552/148‑1 (MQ=255)
                                              ttCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAACAGGCAt                                                                                                  <  1:1989735/148‑1 (MQ=255)
                                               tCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAtt                                                                                                 <  2:1356865/148‑1 (MQ=255)
                                                 aTCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaa                                                                                               >  2:3182900/1‑148 (MQ=255)
                                                     tGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaaaaaa                                                                                           >  1:2325359/1‑148 (MQ=255)
                                                      gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAcc                                                                                                          <  1:1402103/132‑1 (MQ=255)
                                                      gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAcc                                                                                                          >  2:1402218/1‑132 (MQ=255)
                                                      gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAcc                                                                                                          >  2:1402103/1‑132 (MQ=255)
                                                      gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAt                                                                                          >  2:188538/1‑148 (MQ=255)
                                                      gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAt                                                                                          >  2:3457260/1‑148 (MQ=255)
                                                        gtgtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCa                                                                                        <  2:592786/148‑1 (MQ=255)
                                                        gtgtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCa                                                                                        <  2:252101/148‑1 (MQ=255)
                                                        gtgtAAATGATTTAACGAATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCa                                                                                        <  2:3252375/148‑1 (MQ=255)
                                                         tgtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAt                                                                                       >  2:1671392/1‑148 (MQ=255)
                                                             aaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCTTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                   >  1:293983/1‑148 (MQ=255)
                                                               tGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATAATGAgatg                                                                                 >  2:1605903/1‑148 (MQ=255)
                                                                gCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatga                                                                                >  2:2516513/1‑148 (MQ=255)
                                                                   ttAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTa                                                                             <  1:66490/148‑1 (MQ=255)
                                                                       cGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAat                                                                         <  2:849991/148‑1 (MQ=255)
                                                                         cattcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAatat                                                                       <  2:112543/148‑1 (MQ=255)
                                                                          attcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                      <  2:929277/148‑1 (MQ=255)
                                                                          attcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                      <  1:1743935/148‑1 (MQ=255)
                                                                          attcattTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                      >  1:119323/1‑148 (MQ=255)
                                                                              attTAAAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACaa                                                                  >  1:862081/1‑148 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 <  1:3156053/148‑1 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 <  1:240793/148‑1 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 <  2:453802/148‑1 (MQ=255)
                                                                               tttATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAAt                                                                 >  2:3196689/1‑148 (MQ=255)
                                                                                  ataATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAg                                                              <  2:214930/148‑1 (MQ=255)
                                                                                   taATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGa                                                             <  2:1150022/148‑1 (MQ=255)
                                                                                   taATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGa                                                             <  2:1150142/148‑1 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAATTTGCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                        <  2:3384731/148‑1 (MQ=255)
                                                                                        atcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATtata                                                        >  2:797015/1‑148 (MQ=255)
                                                                                         tcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTatat                                                       <  2:2305198/148‑1 (MQ=255)
                                                                                          cccTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATAtt                                                      >  2:1165651/1‑148 (MQ=255)
                                                                                           cattCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCTTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTc                                                     <  1:2577975/146‑1 (MQ=255)
                                                                                               ccATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAAt                                                 >  2:1500389/1‑148 (MQ=255)
                                                                                                cATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATc                                                <  1:1806008/148‑1 (MQ=255)
                                                                                                cATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATc                                                >  2:2290768/1‑148 (MQ=255)
                                                                                                cATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATc                                                >  2:2288809/1‑148 (MQ=255)
                                                                                                cATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATc                                                <  2:1205712/148‑1 (MQ=255)
                                                                                                           taAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGa                                     >  2:3387214/1‑148 (MQ=255)
                                                                                                              gCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGcc                                  >  2:2991846/1‑148 (MQ=255)
                                                                                                              gCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGcc                                  >  2:2992315/1‑148 (MQ=255)
                                                                                                              gCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGcc                                  >  1:1867001/1‑148 (MQ=255)
                                                                                                              gCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGcc                                  >  2:694919/1‑148 (MQ=255)
                                                                                                                aaaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAg                                <  2:2376485/148‑1 (MQ=255)
                                                                                                                aaaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAg                                <  2:3434055/148‑1 (MQ=255)
                                                                                                                    tCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAg                            <  2:77797/148‑1 (MQ=255)
                                                                                                                     ccAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGc                           >  1:1166597/1‑148 (MQ=255)
                                                                                                                     ccAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGc                           >  1:2917944/1‑148 (MQ=255)
                                                                                                                        aataaTACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCtat                        <  2:2493853/145‑1 (MQ=255)
                                                                                                                        aaGAATACATTGATGAAATAACAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCtat                        >  1:1681196/1‑148 (MQ=255)
                                                                                                                              aCATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAATTcc                  >  1:3386328/1‑148 (MQ=255)
                                                                                                                              aCATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTcc                  >  2:1082503/1‑148 (MQ=255)
                                                                                                                              aCATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTcc                  >  2:1886748/1‑148 (MQ=255)
                                                                                                                                     tgaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGc           >  1:1234401/1‑148 (MQ=255)
                                                                                                                                      gaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCt          <  1:1628063/148‑1 (MQ=255)
                                                                                                                                         ataataataAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGc       <  1:3182900/148‑1 (MQ=255)
                                                                                                                                         ataataataAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGc       <  1:188538/148‑1 (MQ=255)
                                                                                                                                              aatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaaa  <  2:2379733/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAA  >  CP009273/2988409‑2988698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG  >  CP009273/2988405‑2988701
                                                                                                                                                     |                                                                                                                                                   
ATCATACACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATA                                                                                                                                                     <  SRR19973065.1319156/150‑1 (MQ=60)
  CATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAA                                                                                                                                                   <  SRR19973065.460476/150‑1 (MQ=60)
  CATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAA                                                                                                                                                   <  SRR19973065.460806/150‑1 (MQ=60)
      CACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATAT                                                                                                                                               <  SRR19973065.1532899/150‑1 (MQ=60)
      CACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATAT                                                                                                                                               <  SRR19973065.1532918/150‑1 (MQ=60)
         GGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAT                                                                                                                                            <  SRR19973065.3364579/150‑1 (MQ=60)
         GGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAT                                                                                                                                            <  SRR19973065.1741646/150‑1 (MQ=60)
           CTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTA                                                                                                                                          <  SRR19973065.980600/150‑1 (MQ=60)
           CTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTA                                                                                                                                          <  SRR19973065.269855/150‑1 (MQ=60)
           CTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTA                                                                                                                                          <  SRR19973065.269767/150‑1 (MQ=60)
           CTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCAAAAGAATACATTGATGAAATAATAATAAAATATAATTA                                                                                                                                          <  SRR19973065.270038/150‑1 (MQ=60)
             GCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAA                                                                                                                                        <  SRR19973065.2757180/150‑1 (MQ=60)
              CTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAA                                                                                                                                       >  SRR19973065.2391331/1‑150 (MQ=60)
              CTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAA                                                                                                                                       >  SRR19973065.3455008/1‑150 (MQ=60)
              CTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAA                                                                                                                                       >  SRR19973065.2291678/1‑150 (MQ=60)
              CTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAA                                                                                                                                       >  SRR19973065.1707808/1‑150 (MQ=60)
               TGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAA                                                                                                                                      >  SRR19973065.389523/1‑150 (MQ=60)
               TGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAA                                                                                                                                      >  SRR19973065.389512/1‑150 (MQ=60)
                GATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAAT                                                                                                                                     >  SRR19973065.556354/1‑150 (MQ=60)
                GATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAAT                                                                                                                                     >  SRR19973065.556296/1‑150 (MQ=60)
                 ATCTGAAACTACACTTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATA                                                                                                                                    <  SRR19973065.2806315/150‑1 (MQ=60)
                  TCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAA                                                                                                                                   <  SRR19973065.2508805/150‑1 (MQ=60)
                   ATGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGATTTAACTCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCAAAAGAATAAATTGATGAAATAATAATAAAATATAATTAAAAATAAA                                                                                                                                  >  SRR19973065.1007453/1‑150 (MQ=60)
                      AAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATT                                                                                                                               <  SRR19973065.923812/150‑1 (MQ=60)
                             AATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGT                                                                                                                        <  SRR19973065.2461516/150‑1 (MQ=60)
                             AATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGT                                                                                                                        <  SRR19973065.819940/150‑1 (MQ=60)
                                          tgctcttccgatctCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCAC                                                                                                           <  SRR19973065.1411295/136‑1 (MQ=60)
                                          tgctcttccgatctCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCAC                                                                                                           <  SRR19973065.1411410/136‑1 (MQ=60)
                                            CATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAG                                                                                                         <  SRR19973065.2379477/150‑1 (MQ=60)
                                            CAAATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATGTATAATCATCCCTTCCATTATTAATATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAAATAAAAATAAAATTAATGCGTAAAAAAATACAACAG                                                                                                         >  SRR19973065.2753840/1‑150 (MQ=60)
                                                  TTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAACAGGCATTA                                                                                                   <  SRR19973065.218177/150‑1 (MQ=60)
                                                  TTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAACAGGCATTA                                                                                                   <  SRR19973065.2002644/150‑1 (MQ=60)
                                                       CCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAA                                                                                              >  SRR19973065.2339916/1‑150 (MQ=60)
                                                               TAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCTTAAAAAAATACCACAGGCATTAAAAAATCATGAGA                                                                                      >  SRR19973065.295878/1‑150 (MQ=60)
                                                                       TTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAA                                                                              <  SRR19973065.67199/150‑1 (MQ=60)
                                                                            GCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATT                                                                         >  SRR19973065.120376/1‑150 (MQ=60)
                                                                              ATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTAC                                                                       <  SRR19973065.1755438/150‑1 (MQ=60)
                                                                                TCATTTAAAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAACAGGCATTAAAAAATCAAGAGATCATTAAAATATTACAA                                                                     >  SRR19973065.867633/1‑150 (MQ=60)
                                                                                   TTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTA                                                                  <  SRR19973065.3175194/150‑1 (MQ=60)
                                                                                   TTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTA                                                                  <  SRR19973065.242514/150‑1 (MQ=60)
                                                                                               CCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAA                                                      <  SRR19973065.823947/150‑1 (MQ=60)
                                                                                               CATTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCTTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAA                                                      <  SRR19973065.2594053/150‑1 (MQ=60)
                                                                                                 TTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAAT                                                    >  SRR19973065.404513/1‑150 (MQ=60)
                                                                                                    CATTATTATTATAAGCAAACTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAT                                                 <  SRR19973065.1817889/150‑1 (MQ=60)
                                                                                                                AAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCC                                     >  SRR19973065.1879290/1‑150 (MQ=60)
                                                                                                                   CAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGG                                  >  SRR19973065.1928379/1‑150 (MQ=60)
                                                                                                                       ATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGC                              >  SRR19973065.1174042/1‑150 (MQ=60)
                                                                                                                       ATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGC                              >  SRR19973065.2935689/1‑150 (MQ=60)
                                                                                                                          CAAAGAATACATTGATGAAATAACAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTAT                           >  SRR19973065.1692444/1‑150 (MQ=60)
                                                                                                                           AAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATA                          >  SRR19973065.268296/1‑150 (MQ=60)
                                                                                                                              GAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGT                       >  SRR19973065.3261812/1‑150 (MQ=60)
                                                                                                                                ATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAATTCC                     >  SRR19973065.3407036/1‑150 (MQ=60)
                                                                                                                                       GATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGC              >  SRR19973065.1242489/1‑150 (MQ=60)
                                                                                                                                          GAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTG           <  SRR19973065.1638975/150‑1 (MQ=60)
                                                                                                                                             ATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCA        <  SRR19973065.3202126/150‑1 (MQ=60)
                                                                                                                                             ATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCA        <  SRR19973065.190014/150‑1 (MQ=60)
                                                                                                                                                   ATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG  >  SRR19973065.631178/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
ATCATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAG  >  CP009273/2988405‑2988701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑