Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,988,554 G→A 100% intergenic (‑103/+119) ygeK ← / ← ygeN pseudogene; putative 2‑component transcriptional regulator/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,988,5540GA96.6% 478.6 / 4.8 146intergenic (‑103/+119)ygeK/ygeNpseudogene; putative 2‑component transcriptional regulator/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (87/54);  total (90/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 150 total aligned reads displayed.

ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACA  >  CP009273/2988408‑2988700
                                                                                                                                                  |                                                                                                                                                  
aTAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATaaaa                                                                                                                                                 <  2:5007266/150‑1 (MQ=255)
    aCGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAAtata                                                                                                                                             <  2:1266142/150‑1 (MQ=255)
     cGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATaa                                                                                                                                            >  1:3069948/1‑150 (MQ=255)
         tgctgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaa                                                                                                                                        >  1:2562624/1‑150 (MQ=255)
            tgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAATATATAATTaaaaa                                                                                                                                     >  1:2122548/1‑150 (MQ=255)
            tgATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTaaaaa                                                                                                                                     >  1:2122807/1‑150 (MQ=255)
               tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAaaaataa                                                                                                                                  >  1:1385357/1‑150 (MQ=255)
               tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataa                                                                                                                                  >  2:926779/1‑150 (MQ=255)
               tCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataa                                                                                                                                  >  2:926716/1‑150 (MQ=255)
                cTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaa                                                                                                                                 <  2:986023/150‑1 (MQ=255)
                 tGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaa                                                                                                                                >  2:1645127/1‑150 (MQ=255)
                 tGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaa                                                                                                                                >  2:1645064/1‑150 (MQ=255)
                 tGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAaaaataaaa                                                                                                                                >  2:4016743/1‑150 (MQ=255)
                   aaaCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtt                                                                                                                              <  2:1281507/150‑1 (MQ=255)
                   aaaCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtt                                                                                                                              >  1:2169486/1‑150 (MQ=255)
                     aCTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAAtttt                                                                                                                            >  1:2760796/1‑150 (MQ=255)
                       tACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                          >  1:1958765/1‑150 (MQ=255)
                       tACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                          >  1:1958875/1‑150 (MQ=255)
                       tACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                          >  1:4866599/1‑150 (MQ=255)
                       tACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTg                                                                                                                          >  1:2352001/1‑150 (MQ=255)
                         cAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCg                                                                                                                        >  1:3946719/1‑150 (MQ=255)
                           attttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTa                                                                                                                      <  2:630446/150‑1 (MQ=255)
                            ttttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaa                                                                                                                     <  1:5024858/150‑1 (MQ=255)
                            ttttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaa                                                                                                                     <  2:1912666/150‑1 (MQ=255)
                            ttttaattttTCCAATATATTCATCCTGAGTTTAAATTCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCTAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaa                                                                                                                     <  1:5163806/150‑1 (MQ=255)
                             tttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaa                                                                                                                    >  1:3996465/1‑150 (MQ=255)
                              ttaattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaa                                                                                                                   >  1:31980/1‑150 (MQ=255)
                               taattttTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTaaaaa                                                                                                                  >  1:313212/1‑150 (MQ=255)
                                  tttttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAt                                                                                                               >  2:1074258/1‑150 (MQ=255)
                                  tttttCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAt                                                                                                               >  2:1074433/1‑150 (MQ=255)
                                      tCCCATATATTCATCCTGAGTGTAAATGCTTTAACTCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCAAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAcaa                                                                                                           <  1:1167109/150‑3 (MQ=255)
                                      tCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACca                                                                                                           >  1:5006962/1‑150 (MQ=255)
                                      tCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGAGTAAAAAAATACca                                                                                                           >  2:581700/1‑150 (MQ=255)
                                        ccATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATacaaca                                                                                                         >  1:1341531/1‑150 (MQ=255)
                                        ccATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACcaca                                                                                                         <  1:2896778/150‑1 (MQ=255)
                                        ccATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAA‑TTTTGCGTAAAAAAATACCACAg                                                                                                        <  1:2370782/150‑1 (MQ=255)
                                           tatatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGc                                                                                                      >  2:2730483/1‑150 (MQ=255)
                                           tatatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGc                                                                                                      <  1:2874196/150‑1 (MQ=255)
                                             tatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAt                                                                                                    >  1:933137/1‑150 (MQ=255)
                                             tatTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAt                                                                                                    <  1:556127/150‑1 (MQ=255)
                                                   tCCTGTGTGTTATTGCTTTATCGCAGTCATTTATTTTCATCCCTTCCTTTTTTTTTTTATGCTAAATCCAAAGAATACTTTGATGAAATTTTATTATAAGATAATTAACATTATAATTTTTGCGTAAAAAAATACCACAGGCATTtaaaa                                                                                              <  2:427066/150‑1 (MQ=255)
                                                    ccTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTaaaaaa                                                                                             >  1:2167985/1‑150 (MQ=255)
                                                     cTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAt                                                                                            >  1:4306499/1‑150 (MQ=255)
                                                     cTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAt                                                                                            >  1:1848587/1‑150 (MQ=255)
                                                       gAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCa                                                                                          <  2:3405977/150‑1 (MQ=255)
                                                        aGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAATAATACATTGATGAAATAATAATAAAATATAATTAAAAATTAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAt                                                                                         <  1:4434791/150‑1 (MQ=255)
                                                        aGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAt                                                                                         >  2:4462042/1‑150 (MQ=255)
                                                        aGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAt                                                                                         <  1:2827995/150‑1 (MQ=255)
                                                         gtgtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATg                                                                                        >  2:3257443/1‑150 (MQ=255)
                                                         gtgtAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATg                                                                                        <  1:4461039/150‑1 (MQ=255)
                                                            tAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATgaga                                                                                     >  2:5006243/1‑150 (MQ=255)
                                                             aaaTGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgat                                                                                    <  1:4095492/150‑1 (MQ=255)
                                                                tGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAgatgat                                                                                 >  2:991684/1‑150 (MQ=255)
                                                                 gatttAACGCTTTCATTTATAATCATCCCTTCAATTATTATTATAAGCAAAATCCAAAGAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGAtt                                                                                <  1:66677/148‑1 (MQ=255)
                                                                 gCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGAtt                                                                                <  1:4623007/150‑1 (MQ=255)
                                                                  cTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCACAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTa                                                                               <  1:2657710/150‑1 (MQ=255)
                                                                  cTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATACTTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTa                                                                               <  1:2110698/150‑1 (MQ=255)
                                                                     tAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTaaaa                                                                            >  2:5042741/1‑150 (MQ=255)
                                                                     tAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTaaaa                                                                            >  1:3739714/1‑150 (MQ=255)
                                                                         gCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                        >  1:4023608/1‑150 (MQ=255)
                                                                         gCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                        >  1:3995776/1‑150 (MQ=255)
                                                                         gCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAtt                                                                        >  2:4061603/1‑150 (MQ=255)
                                                                                     aaTCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATta                                                            <  2:3303073/150‑1 (MQ=255)
                                                                                          tcCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTc                                                       >  1:1019275/1‑150 (MQ=255)
                                                                                             cTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCaaa                                                    >  1:1693938/1‑150 (MQ=255)
                                                                                              ttCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAAt                                                   >  1:481172/1‑150 (MQ=255)
                                                                                                ccATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCa                                                 >  1:3623266/1‑150 (MQ=255)
                                                                                                ccATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCa                                                 >  1:1403613/1‑150 (MQ=255)
                                                                                                   ttattattaTAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTa                                              >  1:347717/1‑150 (MQ=255)
                                                                                                       tattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACt                                          <  2:1534910/150‑1 (MQ=255)
                                                                                                       tattatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACt                                          >  1:2387403/1‑150 (MQ=255)
                                                                                                         ttatAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTg                                        <  1:1581674/150‑1 (MQ=255)
                                                                                                            taAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGc                                     <  2:1520211/150‑1 (MQ=255)
                                                                                                            taAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGc                                     <  2:4096367/150‑1 (MQ=255)
                                                                                                             aaGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGcc                                    <  1:3197319/150‑1 (MQ=255)
                                                                                                               gCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAg                                  <  1:3451752/150‑1 (MQ=255)
                                                                                                                cAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAgg                                 <  2:2217403/150‑1 (MQ=255)
                                                                                                                 aaaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAggg                                >  2:2244327/1‑150 (MQ=255)
                                                                                                                 aaaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAggg                                >  2:2244334/1‑150 (MQ=255)
                                                                                                                  aaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGa                               <  1:1056580/150‑1 (MQ=255)
                                                                                                                  aaaTCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATAACACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAAATTGAGCCAGGGa                               >  2:4758746/1‑150 (MQ=255)
                                                                                                                     tCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCt                            >  1:1505544/1‑150 (MQ=255)
                                                                                                                      ccAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCta                           >  1:4009339/1‑150 (MQ=255)
                                                                                                                         aaGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATaa                        <  1:1602618/150‑1 (MQ=255)
                                                                                                                           gAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGt                      <  1:4631931/150‑1 (MQ=255)
                                                                                                                           gAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGt                      >  1:1454841/1‑150 (MQ=255)
                                                                                                                                cATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCa                 <  2:3739851/150‑1 (MQ=255)
                                                                                                                                cATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCa                 >  1:2417675/1‑150 (MQ=255)
                                                                                                                                 aTTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAg                <  2:2760796/150‑1 (MQ=255)
                                                                                                                                 aTTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAg                >  1:2000436/1‑150 (MQ=255)
                                                                                                                                  ttGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAgg               <  2:3946719/150‑1 (MQ=255)
                                                                                                                                    gattaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGc             <  2:3999505/150‑1 (MQ=255)
                                                                                                                                    gatgaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGc             <  2:3999506/150‑1 (MQ=255)
                                                                                                                                      tgaAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCtt           <  1:3961656/150‑1 (MQ=255)
                                                                                                                                        aaaTAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGc         >  1:1262744/1‑150 (MQ=255)
                                                                                                                                          ataataataAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCa       <  2:1848587/150‑1 (MQ=255)
                                                                                                                                            aataatGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa     <  2:69203/150‑1 (MQ=255)
                                                                                                                                            aataatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCaaa     <  2:5006962/150‑1 (MQ=255)
                                                                                                                                               aatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACa  >  1:1407689/1‑150 (MQ=255)
                                                                                                                                               aatAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACa  >  2:2141642/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACA  >  CP009273/2988408‑2988700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAA  >  CP009273/2988408‑2988703
                                                                                                                                                  |                                                                                                                                                     
ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAA                                                                                                                                                    >  SRR19973063.587681/1‑150 (MQ=60)
     CGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAA                                                                                                                                               >  SRR19973063.3079810/1‑150 (MQ=60)
         TGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAA                                                                                                                                           >  SRR19973063.2570789/1‑150 (MQ=60)
         TGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAA                                                                                                                                           >  SRR19973063.2722465/1‑150 (MQ=60)
            TGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAATATATAATTAAAAA                                                                                                                                        >  SRR19973063.2129182/1‑150 (MQ=60)
            TGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAA                                                                                                                                        >  SRR19973063.2129441/1‑150 (MQ=60)
            TGATCTGAAACTACAATTTTAATTTTTCCCATATATTCAACCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATAATTATAAGAAAAATCCAAAGAAAACATTGATGAAATAATAATAAAATATAATTAAAAA                                                                                                                                        >  SRR19973063.285629/1‑150 (MQ=60)
              ATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATA                                                                                                                                      >  SRR19973063.4014758/1‑150 (MQ=60)
               TCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAA                                                                                                                                     >  SRR19973063.1389546/1‑150 (MQ=60)
                CTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAA                                                                                                                                    >  SRR19973063.1539610/1‑150 (MQ=60)
                   AAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATT                                                                                                                                 >  SRR19973063.2176275/1‑150 (MQ=60)
                     ACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTT                                                                                                                               >  SRR19973063.2769697/1‑150 (MQ=60)
                       TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                             >  SRR19973063.1965004/1‑150 (MQ=60)
                       TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                             >  SRR19973063.2359529/1‑150 (MQ=60)
                       TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                             >  SRR19973063.4882283/1‑150 (MQ=60)
                       TACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTG                                                                                                                             >  SRR19973063.1964894/1‑150 (MQ=60)
                         CAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCG                                                                                                                           >  SRR19973063.3959344/1‑150 (MQ=60)
                            ctctcactccTCCCACATATTCAACCTGAGTCTAAATTCATTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAA                                                                                                                        <  SRR19973063.4837845/140‑1 (MQ=60)
                            TTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAA                                                                                                                        <  SRR19973063.5041064/150‑1 (MQ=60)
                            TTTTAATTTTTCCAATATATTCATCCTGAGTTTAAATTCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCTAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAA                                                                                                                        <  SRR19973063.5180418/150‑1 (MQ=60)
                             TTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAA                                                                                                                       >  SRR19973063.4009280/1‑150 (MQ=60)
                              TTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAA                                                                                                                      >  SRR19973063.32107/1‑150 (MQ=60)
                               TAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAA                                                                                                                     >  SRR19973063.314128/1‑150 (MQ=60)
                                  TTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAAT                                                                                                                  <  SRR19973063.2940553/150‑1 (MQ=60)
                                     TTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACC                                                                                                               >  SRR19973063.1095777/1‑150 (MQ=60)
                                      TCCCATATATTCATCCTGAGTGTAAATGCTTTAACTCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCAAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAA                                                                                                              <  SRR19973063.1170627/150‑1 (MQ=60)
                                      TCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCA                                                                                                              >  SRR19973063.5023127/1‑150 (MQ=60)
                                        CCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACA                                                                                                            <  SRR19973063.2906021/150‑1 (MQ=60)
                                        CCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACA                                                                                                            >  SRR19973063.3192738/1‑150 (MQ=60)
                                        CCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACAACA                                                                                                            >  SRR19973063.1345557/1‑150 (MQ=60)
                                        CCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAA‑TTTTGCGTAAAAAAATACCACAG                                                                                                           <  SRR19973063.2378376/150‑1 (MQ=60)
                                        CCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAA‑TTTTGCGTAAAAAAATACCACAG                                                                                                           <  SRR19973063.2378448/150‑1 (MQ=60)
                                           TATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGC                                                                                                         <  SRR19973063.2883390/150‑1 (MQ=60)
                                             TATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAT                                                                                                       >  SRR19973063.935921/1‑150 (MQ=60)
                                             TATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCAT                                                                                                       <  SRR19973063.557772/150‑1 (MQ=60)
                                                   TCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAA                                                                                                 <  SRR19973063.1414498/150‑1 (MQ=60)
                                                    CCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAA                                                                                                >  SRR19973063.2174770/1‑150 (MQ=60)
                                                    CCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAA                                                                                                >  SRR19973063.2174747/1‑150 (MQ=60)
                                                     CTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAT                                                                                               >  SRR19973063.4320179/1‑150 (MQ=60)
                                                     CTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAAT                                                                                               >  SRR19973063.1854356/1‑150 (MQ=60)
                                                        AGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAATAATACATTGATGAAATAATAATAAAATATAATTAAAAATTAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAT                                                                                            <  SRR19973063.4449023/150‑1 (MQ=60)
                                                        AGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCAT                                                                                            <  SRR19973063.2837082/150‑1 (MQ=60)
                                                         GTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATG                                                                                           <  SRR19973063.4475352/150‑1 (MQ=60)
                                                         GTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATG                                                                                           <  SRR19973063.2120887/150‑1 (MQ=60)
                                                           GTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAG                                                                                         <  SRR19973063.4395964/150‑1 (MQ=60)
                                                             AAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGAT                                                                                       <  SRR19973063.4108617/150‑1 (MQ=60)
                                                              AATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATG                                                                                      <  SRR19973063.3522220/150‑1 (MQ=60)
                                                                 GCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATT                                                                                   <  SRR19973063.4637779/150‑1 (MQ=60)
                                                                 GCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATT                                                                                   <  SRR19973063.450492/150‑1 (MQ=60)
                                                                 GATTTAACGCTTTCATTTATAATCATCCCTTCAATTATTATTATAAGCAAAATCCAAAGAATACATTGATTAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATT                                                                                   <  SRR19973063.66906/150‑1 (MQ=60)
                                                                  CTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCACAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTA                                                                                  <  SRR19973063.2666218/150‑1 (MQ=60)
                                                                  CTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATACTTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTA                                                                                  <  SRR19973063.2117297/150‑1 (MQ=60)
                                                                     TAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAA                                                                               >  SRR19973063.3751661/1‑150 (MQ=60)
                                                                     TAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAA                                                                               >  SRR19973063.2224398/1‑150 (MQ=60)
                                                                        CGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATAT                                                                            <  SRR19973063.5139310/150‑1 (MQ=60)
                                                                         GCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATT                                                                           >  SRR19973063.4008591/1‑150 (MQ=60)
                                                                         GCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATT                                                                           >  SRR19973063.4036515/1‑150 (MQ=60)
                                                                                       TCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATA                                                             >  SRR19973063.1976643/1‑150 (MQ=60)
                                                                                          TCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTC                                                          >  SRR19973063.1022348/1‑150 (MQ=60)
                                                                                             CTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAA                                                       >  SRR19973063.1699098/1‑150 (MQ=60)
                                                                                              TTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAAT                                                      >  SRR19973063.482540/1‑150 (MQ=60)
                                                                                                CCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCA                                                    >  SRR19973063.1407855/1‑150 (MQ=60)
                                                                                                CCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCA                                                    >  SRR19973063.3634893/1‑150 (MQ=60)
                                                                                                CCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCA                                                    >  SRR19973063.1407864/1‑150 (MQ=60)
                                                                                                 CATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCAT                                                   >  SRR19973063.1923528/1‑150 (MQ=60)
                                                                                                   TTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTA                                                 >  SRR19973063.348717/1‑150 (MQ=60)
                                                                                                       TATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACT                                             >  SRR19973063.2395048/1‑150 (MQ=60)
                                                                                                         TTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTG                                           <  SRR19973063.1586525/150‑1 (MQ=60)
                                                                                                          TATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGA                                          <  SRR19973063.4003933/150‑1 (MQ=60)
                                                                                                             AAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCC                                       >  SRR19973063.944853/1‑150 (MQ=60)
                                                                                                             AAGCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCC                                       <  SRR19973063.3207598/150‑1 (MQ=60)
                                                                                                               GCAAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAG                                     <  SRR19973063.3462726/150‑1 (MQ=60)
                                                                                                                 AAAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGG                                   >  SRR19973063.3461849/1‑150 (MQ=60)
                                                                                                                  AAATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGA                                  <  SRR19973063.1059780/150‑1 (MQ=60)
                                                                                                                   AATCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAG                                 >  SRR19973063.4954963/1‑150 (MQ=60)
                                                                                                                     TCCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCT                               >  SRR19973063.1510153/1‑150 (MQ=60)
                                                                                                                      CCAAAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTA                              >  SRR19973063.4022199/1‑150 (MQ=60)
                                                                                                                         AAGAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAA                           <  SRR19973063.1607528/150‑1 (MQ=60)
                                                                                                                           GAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGT                         <  SRR19973063.4646734/150‑1 (MQ=60)
                                                                                                                           GAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGT                         <  SRR19973063.4646775/150‑1 (MQ=60)
                                                                                                                           GAATACATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGT                         >  SRR19973063.1459277/1‑150 (MQ=60)
                                                                                                                                CATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCA                    >  SRR19973063.2425405/1‑150 (MQ=60)
                                                                                                                                 ATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAG                   >  SRR19973063.3948381/1‑150 (MQ=60)
                                                                                                                                 ATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAG                   >  SRR19973063.2006684/1‑150 (MQ=60)
                                                                                                                                 ATTGATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAG                   >  SRR19973063.1305590/1‑150 (MQ=60)
                                                                                                                                    GATGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGC                >  SRR19973063.4036757/1‑150 (MQ=60)
                                                                                                                                      TGAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTT              <  SRR19973063.3974332/150‑1 (MQ=60)
                                                                                                                                       GAAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTG             >  SRR19973063.256179/1‑150 (MQ=60)
                                                                                                                                        AAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGC            >  SRR19973063.16840/1‑150 (MQ=60)
                                                                                                                                        AAATAATAATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGC            >  SRR19973063.1266501/1‑150 (MQ=60)
                                                                                                                                               AATAAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACA     >  SRR19973063.1411952/1‑150 (MQ=60)
                                                                                                                                                  AAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAA  >  SRR19973063.2246592/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
ATAAACGGCTGCTGATCTGAAACTACAATTTTAATTTTTCCCATATATTCATCCTGAGTGTAAATGCTTTAACGCATTCATTTATAATCATCCCTTCCATTATTATTATAAGCAAAATCCAAAGAATACATTGATGAAATAATAATGAAATATAATTAAAAATAAAATTTTTGCGTAAAAAAATACCACAGGCATTAAAAAATCATGAGATGATTAAAATATTACAATTAGATTATATTCAAATCATTAAACTTGAGCCAGGGAGCTATAAGTCCTCAGGGCTTGCCAAAACAGAA  >  CP009273/2988408‑2988703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑