breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009134,410,0441.→T18.5% 18.8 0.0 ‑0.027intergenic (+38/‑89)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
*NC_0009133,508,8940G→A10.5% 11.6 ‑4.9 0.096A307V (GCA→GTA) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
*NC_0009133,799,2900T→C100.0% 3.7 3K8R (AAA→AGA) waaUlipopolysaccharide core biosynthesis
*NC_0009133,621,0450T→G100.0% 1.3 1D618E (GAT→GAGrhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009133,622,1970A→.100.0% 1.3 1coding (3006/4236 nt)rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009133,942,2280T→A100.0% 1.2 1noncoding (421/1542 nt)rrsC16S ribosomal RNA of rrnC operon
*NC_0009132,729,7570C→A100.0% 1.1 1noncoding (1401/1542 nt)rrsG16S ribosomal RNA of rrnG operon
*NC_0009131,432,4310A→C100.0% 0.8 1E7D (GAA→GACtfaRRac prophage; putative tail fiber assembly protein
*NC_0009132,101,9250T→A100.0% 0.7 1Q329H (CAA→CATinsH1IS5 transposase and trans‑activator
*NC_0009133,621,0540A→G100.0% 0.4 1G621G (GGA→GGGrhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009133,622,1800C→G100.0% 0.3 1L997V (CTG→GTG) rhsBRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
*NC_0009131,634,4610T→A100.0% ‑0.5 1E142V (GAG→GTG) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4620C→A100.0% ‑0.5 1E142* (GAG→TAG) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4640C→A100.0% ‑0.5 1S141I (AGT→ATT) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4650T→A100.0% ‑0.5 1S141C (AGT→TGT) tfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4660G→A100.0% ‑0.5 1A140A (GCC→GCTtfaQQin prophage; putative tail fibre assembly protein
*NC_0009131,634,4670G→A100.0% ‑0.5 1A140V (GCC→GTC) tfaQQin prophage; putative tail fibre assembly protein

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009132,074,3570C→.10.4% 18.1 ‑0.4 ‑0.067coding (2819/3120 nt)fluCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
*NC_0009134,296,0600C→T26.4% 97.0 ‑0.2 ‑0.3129intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3619878 =NA (NA)10 (0.110) 7/286 8.3 100% coding (687/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3619892 = 0 (0.000)coding (701/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 3327193 =77 (0.810)6 (0.070) 6/286 8.8 7.8% coding (472/630 nt) rlmE 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent
?NC_000913 3327230 = 70 (0.790)coding (435/630 nt) rlmE 23S rRNA U2552 2'‑O‑ribose methyltransferase, SAM‑dependent
* ? NC_000913 = 3858205113 (1.190)5 (0.060) 5/278 9.2 4.8% coding (200/1494 nt) yidJ sulfatase/phosphatase superfamily protein
?NC_000913 = 3858228 93 (1.070)coding (177/1494 nt) yidJ sulfatase/phosphatase superfamily protein
* ? NC_000913 = 381689NA (NA)5 (0.060) 4/286 10.0 NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 = 132979285 (0.900)4 (0.040) 4/292 10.2 4.9% coding (461/1050 nt) sohB inner membrane protein, S49 peptidase family protein
?NC_000913 = 1329859 73 (0.800)coding (528/1050 nt) sohB inner membrane protein, S49 peptidase family protein
* ? NC_000913 2729457 =66 (0.700)4 (0.040) 4/288 10.1 5.8% intergenic (‑13/+159) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 2729476 = 67 (0.750)intergenic (‑32/+140) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
* ? NC_000913 = 198744190 (0.950)4 (0.050) 3/284 10.7 4.7% intergenic (+13/+66) ftnB/yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein
?NC_000913 = 1987450 80 (0.900)intergenic (+22/+57) ftnB/yecJ ferritin B, putative ferrous iron reservoir/DUF2766 family protein
* ? NC_000913 = 335560679 (0.830)4 (0.040) 3/288 10.8 4.7% coding (882/4461 nt) gltB glutamate synthase, large subunit
?NC_000913 = 3355642 87 (0.970)coding (918/4461 nt) gltB glutamate synthase, large subunit
* ? NC_000913 225456 =18 (0.190)3 (0.030) 3/286 10.7 13.7% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 225477 = 21 (0.240)intergenic (+20/‑23) ileV/alaV tRNA‑Ile/tRNA‑Ala
* ? NC_000913 225456 =18 (0.190)3 (0.040) 3/260 10.1 5.9% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 2518117 94 (0.990)intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
* ? NC_000913 225456 =18 (0.190)3 (0.040) 3/258 10.0 5.4% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 2518231 107 (1.130)noncoding (1/76 nt) alaW tRNA‑Ala
* ? NC_000913 225456 =18 (0.190)3 (0.030) 3/286 10.7 15.0% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 3427056 NA (NA)intergenic (‑23/+20) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 225468 =21 (0.220)3 (0.030) 3/286 10.7 13.2% intergenic (+11/‑32) ileV/alaV tRNA‑Ile/tRNA‑Ala
?NC_000913 = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 381292 =NA (NA)3 (0.030) 3/286 10.7 NA noncoding (33/1331 nt) IS2 repeat region
?NC_000913 381312 = NA (NA)noncoding (53/1331 nt) IS2 repeat region
* ? NC_000913 1997022 =55 (0.580)3 (0.030) 3/284 10.7 5.0% intergenic (‑190/+40) sdiA/yecC quorum‑sensing transcriptional activator/putative ABC transporter ATPase
?NC_000913 1997051 = 63 (0.710)intergenic (‑219/+11) sdiA/yecC quorum‑sensing transcriptional activator/putative ABC transporter ATPase
* ? NC_000913 = 209158984 (0.890)3 (0.030) 3/286 10.7 3.3% coding (493/1305 nt) hisD bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
?NC_000913 = 2091646 98 (1.100)coding (550/1305 nt) hisD bifunctional histidinal dehydrogenase/ histidinol dehydrogenase
* ? NC_000913 = 251811794 (0.990)3 (0.040) 3/260 10.1 3.6% intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile
* ? NC_000913 = 2518231107 (1.130)3 (0.040) 3/258 10.0 3.2% noncoding (1/76 nt) alaW tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile
* ? NC_000913 2729457 =66 (0.700)4 (0.040) 3/288 10.8 5.4% intergenic (‑13/+159) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168340 77 (0.860)intergenic (+140/‑32) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 2729468 =69 (0.730)4 (0.040) 3/288 10.8 5.4% intergenic (‑24/+148) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168351 75 (0.840)intergenic (+151/‑21) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 = 2730046NA (NA)3 (0.030) 3/302 11.1 NA noncoding (1112/1542 nt) rrsG 16S ribosomal RNA of rrnG operon
?NC_000913 2730050 = NA (NA)noncoding (1108/1542 nt) rrsG 16S ribosomal RNA of rrnG operon
* ? NC_000913 3060398 =81 (0.860)3 (0.030) 3/288 10.8 3.7% coding (646/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
?NC_000913 3060457 = 79 (0.880)coding (705/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
* ? NC_000913 = 3427065NA (NA)3 (0.030) 3/286 10.7 NA intergenic (‑32/+11) alaU/ileU tRNA‑Ala/tRNA‑Ile
?NC_000913 = 3427068 NA (NA)intergenic (‑35/+8) alaU/ileU tRNA‑Ala/tRNA‑Ile
* ? NC_000913 4067707 =97 (1.020)3 (0.030) 3/286 10.7 3.3% coding (1570/2037 nt) yihQ putative sulpholipid alpha‑glucosidase; alpha‑glucosyl fluoride glucosidase
?NC_000913 4067734 = 83 (0.930)coding (1543/2037 nt) yihQ putative sulpholipid alpha‑glucosidase; alpha‑glucosyl fluoride glucosidase