Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
770 |
51.4 |
2439994 |
93.8% |
2288714 |
110.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,511,746 |
(A)8→9 |
coding (599/906 nt) |
yhfZ ← |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,511,738 | 1 | . | A | 94.7%
| 116.1
/ ‑0.3
| 38 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (21/15); total (22/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01 |
CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511601‑3511869
|
cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a > 2:582699/1‑139 (MQ=255)
cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa > 1:1082103/1‑139 (MQ=255)
cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa > 1:973083/1‑139 (MQ=255)
tctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca < 1:758469/139‑4 (MQ=255)
tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCt > 2:311263/1‑139 (MQ=255)
aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:973083/139‑1 (MQ=255)
aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACgcgc > 2:1140604/1‑139 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa > 2:367483/1‑96 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa < 1:367483/96‑1 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACCCGCtt > 2:317283/1‑139 (MQ=255)
cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:435950/117‑1 (MQ=255)
cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 1:435950/1‑117 (MQ=255)
cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGt > 1:710281/1‑139 (MQ=255)
cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:331981/113‑1 (MQ=255)
cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 1:331981/1‑113 (MQ=255)
tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:385936/1‑124 (MQ=255)
tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:385936/124‑1 (MQ=255)
cGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCgg > 1:1046286/1‑139 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg < 1:1046441/90‑1 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg > 2:1046441/1‑90 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg > 2:745213/1‑139 (MQ=255)
ctctCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc < 1:745213/139‑1 (MQ=255)
tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca < 2:421383/52‑4 (MQ=255)
tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaaaaaaca > 1:421383/1‑49 (MQ=255)
cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt > 1:426897/1‑139 (MQ=255)
gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 1:582699/139‑1 (MQ=255)
aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 1:311263/139‑1 (MQ=255)
agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:571984/72‑1 (MQ=255)
agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:234452/72‑1 (MQ=255)
agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:571984/1‑72 (MQ=255)
agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:234452/1‑72 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:990282/1‑139 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:1101074/1‑139 (MQ=255)
tCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:1073666/129‑1 (MQ=255)
tCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 1:1073666/1‑129 (MQ=255)
aCTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc < 1:540814/99‑1 (MQ=255)
aCTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc > 2:540814/1‑99 (MQ=255)
cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtgt > 1:362542/1‑139 (MQ=255)
cacaTCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGccc < 1:1140604/139‑1 (MQ=255)
tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTATGGCAAATCAACTGGTGCTc < 1:884060/110‑1 (MQ=255)
tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTATGGCAAATCAACTGGTGCTc > 2:884060/1‑110 (MQ=255)
tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:283858/1‑139 (MQ=255)
ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt < 2:21682/50‑1 (MQ=255)
ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTAt > 1:21682/1‑50 (MQ=255)
|
CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511601‑3511869
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
