Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,511,746 |
(A)8→9 |
coding (599/906 nt) |
yhfZ ← |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,511,738 | 1 | . | A | 94.8%
| 175.4
/ 2.7
| 58 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base A (27/28); total (28/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 |
GCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511603‑3511869
|
gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaa > 2:1010280/1‑139 (MQ=255)
cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa > 1:9282/1‑139 (MQ=255)
cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCACCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaa > 2:419590/1‑139 (MQ=255)
cccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTc < 1:1265734/139‑1 (MQ=255)
aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCACCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATg < 1:419590/139‑1 (MQ=255)
ggTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGAtttt < 1:373629/139‑1 (MQ=255)
tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCt > 2:1285156/1‑139 (MQ=255)
gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGAt < 2:9282/139‑1 (MQ=255)
ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATc > 2:60718/1‑139 (MQ=255)
ttttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATccgcc < 1:1010280/139‑1 (MQ=255)
gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:1120946/139‑1 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa < 1:40240/132‑1 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa > 2:40240/1‑132 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:79546/1‑139 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a < 1:291569/79‑1 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a > 2:291569/1‑79 (MQ=255)
aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt < 2:79546/139‑1 (MQ=255)
gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAGAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:839619/139‑1 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 2:1003440/127‑1 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 1:1003440/1‑127 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg > 1:183605/1‑139 (MQ=255)
gCGTTGGAAACTCTCGTGATAAGAGAGAGCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg < 2:1068894/139‑1 (MQ=255)
tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa > 1:1260360/1‑139 (MQ=255)
tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa > 2:972115/1‑139 (MQ=255)
ctctCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc < 1:60718/139‑1 (MQ=255)
tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACt > 1:1205896/1‑139 (MQ=255)
tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:566498/97‑1 (MQ=255)
tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:566498/1‑97 (MQ=255)
cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt > 1:929315/1‑139 (MQ=255)
cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt < 2:1260360/139‑1 (MQ=255)
cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:675315/1‑139 (MQ=255)
tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAc > 1:699953/1‑93 (MQ=255)
tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAc < 2:699953/93‑1 (MQ=255)
gATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:675315/139‑1 (MQ=255)
aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:1205896/139‑1 (MQ=255)
agagagATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGcc < 2:183605/139‑1 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:1227271/1‑128 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:1227271/128‑1 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 1:608627/1‑68 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:608627/68‑1 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:622141/83‑1 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:622141/1‑83 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:744013/1‑139 (MQ=255)
tCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 2:868944/1‑99 (MQ=255)
tCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:868944/99‑1 (MQ=255)
cGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc < 1:1025310/112‑1 (MQ=255)
cGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc > 2:1025310/1‑112 (MQ=255)
tCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg > 1:1083748/1‑139 (MQ=255)
gTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg < 2:1083748/139‑1 (MQ=255)
tCCACATCACTACCGCCAAAAAA‑AAACATCGGTCa > 1:528150/1‑35 (MQ=255)
tCCACATCACTACCGCCAAAAAA‑AAACATCGGTCa < 2:528150/35‑1 (MQ=255)
cTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:645998/1‑64 (MQ=255)
cTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc > 1:298944/1‑113 (MQ=255)
cTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:298944/113‑1 (MQ=255)
cTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 2:547292/1‑139 (MQ=255)
cTACCACCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:645998/64‑1 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc > 1:1028134/1‑53 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc < 2:1028134/53‑1 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:426484/1‑107 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:426484/107‑1 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:10582/1‑139 (MQ=255)
ccGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa < 1:27087/59‑1 (MQ=255)
ccGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa > 2:27087/1‑59 (MQ=255)
|
GCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511603‑3511869
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
