Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,746	(A)_8→9	coding (599/906 nt)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,738	1	.	A	100.0%	139.9 / NA	44	G203C (GGC→TGC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (24/20); total (24/20)

CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511601‑3511869

cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑a                                                                                                                                    <  1:645559/139‑1 (MQ=255)

  gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaa                                                                                                                                  >  1:363548/1‑139 (MQ=255)

            tAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaacatc                                                                                                                        <  2:472316/139‑6 (MQ=255)

                          tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGCTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCt                                                                                                           >  2:770402/1‑139 (MQ=255)

                          tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGATGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCt                                                                                                           >  2:480110/1‑139 (MQ=255)

                             gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaacat                                                                                                                         >  1:1015710/1‑117 (MQ=255)

                             gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaacat                                                                                                                         <  2:1015710/121‑5 (MQ=255)

                                   tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:583032/1‑139 (MQ=255)

                                        gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  2:221727/139‑1 (MQ=255)

                                        gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                             <  1:770402/139‑1 (MQ=255)

                                            ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  <  1:19456/130‑1 (MQ=255)

                                            ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                  >  2:19456/1‑130 (MQ=255)

                                                            cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGCTCGACTCGTTCCCCACCACTACCGCCA‑AAAAAAAACATCGGCCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                         >  1:1042896/1‑139 (MQ=255)

                                                              cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                       <  1:583032/139‑1 (MQ=255)

                                                              cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                       >  1:762887/1‑139 (MQ=255)

                                                                             tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc                                                        >  1:67501/1‑139 (MQ=255)

                                                                             tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc                                                        >  1:742077/1‑139 (MQ=255)

                                                                              aaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGcc                                                       >  2:1034381/1‑139 (MQ=255)

                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGa                                                                                                         <  1:712275/84‑1 (MQ=255)

                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGa                                                                                                         >  2:712275/1‑84 (MQ=255)

                                                                                      gTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                               <  2:67501/139‑1 (MQ=255)

                                                                                        tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa                                             >  2:681587/1‑139 (MQ=255)

                                                                                               tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACt                                      >  1:760423/1‑139 (MQ=255)

                                                                                                  cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt                                   >  2:403245/1‑139 (MQ=255)

                                                                                                   gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACTCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                  <  2:760423/139‑1 (MQ=255)

                                                                                                    tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                                 >  1:63886/1‑139 (MQ=255)

                                                                                                        aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  1:480110/139‑1 (MQ=255)

                                                                                                        aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTCATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  2:681629/139‑1 (MQ=255)

                                                                                                        aaGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTAATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                             <  1:681587/139‑1 (MQ=255)

                                                                                                         agagagATCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGcc                            <  2:327244/139‑1 (MQ=255)

                                                                                                            gagaTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAAc                         <  1:403245/139‑1 (MQ=255)

                                                                                                               aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGt                       >  2:500787/1‑139 (MQ=255)

                                                                                                               aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCa                                                                                                                    >  2:156322/1‑45 (MQ=255)

                                                                                                               aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCa                                                                                                                    <  1:156322/45‑1 (MQ=255)

                                                                                                               aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                      >  2:96336/1‑139 (MQ=255)

                                                                                                               aTCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                      >  2:1069489/1‑139 (MQ=255)

                                                                                                                tCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  2:604905/82‑1 (MQ=255)

                                                                                                                tCGACTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              >  1:604905/1‑82 (MQ=255)

                                                                                                                    cTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  1:243397/78‑1 (MQ=255)

                                                                                                                    cTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              >  2:243397/1‑78 (MQ=255)

                                                                                                                    cTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              >  2:561897/1‑78 (MQ=255)

                                                                                                                    cTCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  1:561897/78‑1 (MQ=255)

                                                                                                                     tCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg                <  2:63886/139‑1 (MQ=255)

                                                                                                                     tCGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg                <  1:1069489/139‑1 (MQ=255)

                                                                                                                      cGTTCCACATCACTACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc               <  1:915226/139‑1 (MQ=255)

                                                                                                                                   tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              >  2:7643/1‑63 (MQ=255)

                                                                                                                                   tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                              <  1:7643/63‑1 (MQ=255)

                                                                                                                                   tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  2:196305/1‑139 (MQ=255)

                                                                                                                                   tACCGCCA‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa  >  1:896525/1‑139 (MQ=255)

CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA  >  NC_000913/3511601‑3511869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A