Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
758 |
59.3 |
2864096 |
93.9% |
2689386 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,511,746 |
(A)8→9 |
coding (599/906 nt) |
yhfZ ← |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,511,738 | 1 | . | A | 96.1%
| 157.7
/ ‑0.6
| 51 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (26/23); total (27/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01 |
GCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAG > NC_000913/3511621‑3511870
|
gCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaa‑aaaacat > 2:407392/1‑126 (MQ=255)
gCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaaaa‑aaaacat < 1:407392/130‑5 (MQ=255)
aTGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTc < 1:999802/133‑1 (MQ=255)
aTGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTc > 2:999802/1‑133 (MQ=255)
ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATc > 2:1087780/1‑139 (MQ=255)
ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCGTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATc < 1:1094985/139‑1 (MQ=255)
tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATccgccg < 2:1358049/139‑1 (MQ=255)
gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:1061673/139‑1 (MQ=255)
gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:265738/139‑1 (MQ=255)
gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:1061673/1‑139 (MQ=255)
caccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGtt > 2:1054725/1‑139 (MQ=255)
aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:1221627/1‑139 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 2:1221692/1‑139 (MQ=255)
cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa > 1:739380/1‑139 (MQ=255)
aCATGGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGGACGGTTATCCAGCCCCACGCGCTTCACGttt > 2:598390/1‑139 (MQ=255)
tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg < 1:1054725/139‑1 (MQ=255)
tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc > 2:530365/1‑139 (MQ=255)
cAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCtt < 1:598390/139‑1 (MQ=255)
aTGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTa > 1:701735/1‑139 (MQ=255)
tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:334339/111‑1 (MQ=255)
tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc > 1:340800/1‑139 (MQ=255)
tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGCTTATCCAGCCCCAcg > 1:334339/1‑111 (MQ=255)
gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg > 2:252925/1‑139 (MQ=255)
tGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaa > 1:1255224/1‑139 (MQ=255)
tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc < 1:252925/139‑1 (MQ=255)
tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc > 1:495517/1‑139 (MQ=255)
gATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCt < 2:701735/139‑1 (MQ=255)
aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:530365/139‑1 (MQ=255)
aaGAGAGATTGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGGCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 1:1312204/139‑1 (MQ=255)
gagagaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCa < 1:1087780/139‑1 (MQ=255)
agagaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCACACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc > 1:1113520/1‑136 (MQ=255)
agagaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCAAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:1113520/136‑1 (MQ=255)
agaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCTACTGGTGCTCGCCAACg > 2:1205262/1‑139 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGAt < 2:1231535/58‑1 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGAt > 1:1231535/1‑58 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc > 1:1398581/1‑123 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc < 2:1398581/123‑1 (MQ=255)
aTCGCCTCGTTCCACATCACTACCGCCAAAAAA‑AAACTTCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:1036697/1‑139 (MQ=255)
aTCGACTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 1:722524/1‑139 (MQ=255)
cTCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtgt < 1:229265/139‑1 (MQ=255)
tCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 2:340800/139‑1 (MQ=255)
tCGTTCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 2:739380/139‑1 (MQ=255)
ttCCACATCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCCCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCgg > 2:1373542/1‑139 (MQ=255)
aTCACTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCaa > 1:1314723/1‑139 (MQ=255)
aCTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:681445/1‑109 (MQ=255)
aCTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:681445/109‑1 (MQ=255)
aCTACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTc < 2:722524/139‑1 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc > 1:733280/1‑94 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc < 2:733280/94‑1 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 2:769535/1‑110 (MQ=255)
tACCGCCAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 1:769535/110‑1 (MQ=255)
tACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAg > 1:685865/1‑139 (MQ=255)
gCC‑AAAAATAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt < 2:936350/71‑1 (MQ=255)
|
GCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAG > NC_000913/3511621‑3511870
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
