Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,511,746 |
(A)8→9 |
coding (599/906 nt) |
yhfZ ← |
putative DNA‑binding transcriptional regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,511,738 | 1 | . | A | 95.2%
| 127.3
/ ‑0.6
| 42 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (25/15); total (26/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
CTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑C‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC > NC_000913/3511612‑3511871
|
ctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑C‑aaaaa‑aaaacat < 1:591293/139‑5 (MQ=255)
ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCa < 1:526829/139‑1 (MQ=255)
ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGCTGTAACCTCTCGTGATAAGAGAGATCGACGCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCa < 2:833399/139‑1 (MQ=255)
aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATg < 1:1006340/139‑1 (MQ=255)
ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATc > 1:859512/1‑139 (MQ=255)
tCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATcc > 2:434265/1‑139 (MQ=255)
tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATccgccg > 1:981900/1‑139 (MQ=255)
aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACgcgc > 1:530510/1‑139 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg > 2:905336/1‑119 (MQ=255)
cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:905336/119‑1 (MQ=255)
cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa < 2:796988/136‑1 (MQ=255)
cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa > 1:796988/1‑136 (MQ=255)
cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGt > 2:268081/1‑139 (MQ=255)
gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACCCGTTCCCAATGACTACCGCGT‑AAAAA‑AAGCATCGGGCATGATTTTCTCATCCGCGGAATGGTTATTCAGCCCCACGCGCTTCACGTTTGCGGa < 2:596465/139‑1 (MQ=255)
ctctCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc < 2:859512/139‑1 (MQ=255)
cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt > 2:547981/1‑139 (MQ=255)
gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 1:651715/139‑1 (MQ=255)
tGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc > 1:608401/1‑139 (MQ=255)
aaGAGAGATCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc < 2:27907/139‑1 (MQ=255)
agaTCGACTCGTTCCACATCACTACCGT‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg < 2:608401/139‑1 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa > 2:56118/1‑82 (MQ=255)
gaTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa < 1:56118/82‑1 (MQ=255)
aTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 1:1008646/127‑1 (MQ=255)
aTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 2:1008646/1‑127 (MQ=255)
aTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 1:114997/1‑139 (MQ=255)
aTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCC‑ACGTAg > 1:386889/1‑139 (MQ=255)
aTCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGAAAATCAACTGGTGCTCGCCAACGTa > 2:857998/1‑139 (MQ=255)
tCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc > 1:833137/1‑129 (MQ=255)
tCGACTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:833137/129‑1 (MQ=255)
cTCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgtgt > 1:465858/1‑139 (MQ=255)
tCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg > 2:123500/1‑139 (MQ=255)
tCGTTCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 1:356224/139‑1 (MQ=255)
tCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc > 1:68421/1‑139 (MQ=255)
tCCACATCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc > 2:180495/1‑139 (MQ=255)
acaTCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcccc < 2:68421/139‑1 (MQ=255)
caTCACTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCa > 1:268059/1‑139 (MQ=255)
aCTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTc > 2:591554/1‑139 (MQ=255)
cTACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 2:994578/1‑139 (MQ=255)
tACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCCCGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCCCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 2:50633/1‑139 (MQ=255)
tACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:775301/107‑1 (MQ=255)
tACCGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:775301/1‑107 (MQ=255)
tACCGC‑C‑AAAAAGAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:801090/1‑139 (MQ=255)
ccGC‑CAAAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTAACGTTTGCGGACTCCCCTTTACGGCAAATCAACTGGTCCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc > 2:530325/1‑139 (MQ=255)
|
CTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGC‑C‑AAAAA‑AAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC > NC_000913/3511612‑3511871
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
