Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA100.0% 423.5 / ‑6.7 125P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (69/50);  total (74/51)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 126 total aligned reads displayed.

TGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAA  >  NC_000913/4184305‑4184777
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
tGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGGATATCTGTCGGTTAAACGCCGTATCCAGCCGGGTGACAAAATGGCCGGTCGTCCCGGGAACAAGGGTGTAA‑TTTCTAAGCTCAACCCGCACGACGATATGCCTTACGAGGCAAAAGGTAAGCCGGGAGAACTCGTACTGAACCCg                                                                                                                                                                                                                                             >  2:636208/1‑239 (MQ=255)
    acgagAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACACAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                                                                                                                         <  1:184754/235‑1 (MQ=255)
    acaccgggtaggaaaaaaaCCCGAAGCGAAACGCAGCAAACTTCCCCAGGGCGACGCACTGGCCCCGGGCGTTCGGAAGATTGGTAAGGTATATGTGGCGGTTAAACGCCGGATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                                                                                                                         <  2:600902/223‑1 (MQ=255)
            tagaaaaaaaacctgaggcaaaaCGCCGGAAAATCTCCCAGGGCGACGGGGTGGCACCGGGGGGGCGGAAGAGTTTTAAGGTATATCGGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                                                                                                                                  <  2:862667/218‑1 (MQ=255)
             cGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCg                                                                                                                                                                                                                                 >  1:46332/1‑238 (MQ=255)
                gaaaaaACTCGAAGCGAAACGCCGCAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtct                                                                                                                                                                                                                              <  1:731320/238‑1 (MQ=255)
                              cGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGGAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCCCGAATGAACATCgg                                                                                                                                                                                                                >  1:281209/1‑238 (MQ=255)
                                   cgccccAAAATCCCCCAGGGGTACGATCTGGCACCGGGGGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                                                                                                                          <  2:140408/239‑1 (MQ=255)
                                                cccGGGGTGGCGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCGGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCAc                                                                                                                                                                                            <  2:46332/239‑1 (MQ=255)
                                                     ggCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCCGGGCGAACCGTCCCGTATGAAAATCGGTCAAAACCC‑CGAAACCCACCCggg                                                                                                                                                                                       >  2:210567/1‑239 (MQ=255)
                                                          cgaTCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGGATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGAGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTAAGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAACCGGGGGTAACGCCTCGTATGAACATACGTAAAAGCCA‑CAAAACCCACCCgggacagg                                                                                                                                                                                  >  2:509658/1‑234 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCCGGACGTCACGGTAACAAGGGTGCAA‑TTTCTCAGCTCACCCCGCTCGAAGATATGCCTTACGATGAAACCAGTACGCCGGTATAAATCGCACTGAACACGCTGGGCGGACCGGCCAGTCGGCAAATAGTACAGATCGC‑CCAAACCCAACTGGGGATgggggcccaaggca                                                                                                                                                                      >  2:588214/1‑226 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCCACTGGGTATGGCGTCGAAAGGTaa                                                                                                                                                                     >  2:1010291/1‑238 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGGAt                                                                                                                                                                     >  1:223867/1‑239 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGACTGAAAATCGGTCAGATCCT‑CGAAACCCCCCTGGGTAGAGCGGCGAAAggga                                                                                                                                                                      >  1:548025/1‑236 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGGCTCGAATGAACATCGGTCAGATCTC‑CGAAACCCACCCGGGTATGGCCGGGAAAggaa                                                                                                                                                                      >  2:743081/1‑236 (MQ=255)
                                                                       ggCGTGCTGAAGATTGTTAAGGGATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAAACCGATCGAAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTCCTGAACCAACTGGGCGGCCCGCCTCGTATGAACATCGGTCAGATCCT‑CGAAACACACCTGGGCATgggagggaaggggaa                                                                                                                                                                     >  2:826911/1‑226 (MQ=255)
                                                                           tGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCGAATCCT‑CGAAACCCACCTGGGGATGGCGCGGAAAGGTATCgcc                                                                                                                                                                 >  1:409592/1‑237 (MQ=255)
                                                                           tGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCCCCTTGGCATGGCTGCGAAAGGGAtacgc                                                                                                                                                                 >  1:987026/1‑235 (MQ=255)
                                                                            gCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGGACTTAACCCGCTGGGCGTACAGTCTCGTATGAACATCGGTCAGATCCA‑CGAAACCCACCTGGGTATGGCTGGTAAAggaaagggcg                                                                                                                                                                >  2:132788/1‑231 (MQ=255)
                                                                               taaGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                                                                                                                              <  1:743081/237‑1 (MQ=255)
                                                                               gAAGTTTTTAAAGGTATATCGGGGGGTTAAACGCCGTAACCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                                                                                                                              <  1:313294/238‑1 (MQ=255)
                                                                                     tGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCa                                                                                                                                                       >  1:763914/1‑239 (MQ=255)
                                                                                       ttAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCaa                                                                                                                                                      >  1:609825/1‑238 (MQ=255)
                                                                                        tAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGAGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGGTGAAAACGGTACGCCGTAAGACATCGTACTGCACCAGCTCGGCGTCCCGTCTCGTATGACCACCGGTCAGAACCT‑CGAAACCCACACGGGTTTGGCTGATAAAgcactagcggaacaaaacaac                                                                                                                                                     >  2:402412/1‑218 (MQ=255)
                                                                                         aaGGTATCTCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                                                                                                                   <  1:980477/239‑1 (MQ=255)
                                                                                            gTATATCTGGCGGTTAACCGCCGTATCCAGCCTGGGGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGACGATATGCCTTACGCTGAAAACGGTCCGCCGGTAGCCATCGTACTGAACCACCTGGGCTTACAGTCTCGTCTGAAGCACGGACAGATCCC‑CGAAACACCCCTGGGGATGGCTCGTCAAGGTATCGGCCACAAAACCaaaccca                                                                                                                                                 >  2:21570/1‑233 (MQ=255)
                                                                                              atatCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                                                                                                                              <  1:1010291/239‑1 (MQ=255)
                                                                                               tatCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCCAAAAGACCAACGCCAt                                                                                                                                                >  1:944543/1‑236 (MQ=255)
                                                                                               tatCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAAATCCT‑CGAAACCCCCCTGGGTATGGCTGCGGAAGGCATCGGCGCCAAAATCAACCCCacccc                                                                                                                                             >  2:561254/1‑235 (MQ=255)
                                                                                                 tCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTTGGCGTACCCCCTCGTATGAACATCGGGCCGCTCCC‑CGAAACCCACCTGGGAATGGCTGCCGAAGGAATCGGCGACaaagtaaaaccccagtcaa                                                                                                                                           >  2:199571/1‑222 (MQ=255)
                                                                                                 tCTGGCGGTTAAACGCCATATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGGAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCGGGGCGTACCGTCTAGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGGATGCGTGAGAAAGGAATCGGGCACAAAATACACGCCacgacga                                                                                                                                           >  2:469391/1‑233 (MQ=255)
                                                                                                    ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                                                                                                                        <  1:199571/239‑1 (MQ=255)
                                                                                                    ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGCAACTAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCCCCTGGGTATGGCTGCGAAAGGGATCGGCTACAAGATGAACGCCATGATTAAAc                                                                                                                                        >  1:852401/1‑239 (MQ=255)
                                                                                                      cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                                                                                                                                    >  2:868095/1‑162 (MQ=255)
                                                                                                      cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                                                                                                                                    <  1:868095/162‑1 (MQ=255)
                                                                                                      cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGCCAAGATCAACGCCATGCTGAAAcag                                                                                                                                      >  1:52695/1‑239 (MQ=255)
                                                                                                      cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGGGTAATTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGGACCGTCTCGACTGCACATCGGTCAGATCCT‑CGAAACCCACCCTGGTATGGCTGCGCAAGGTCTCGGGGACCAGAACAACGCAACGCTGaaaaa                                                                                                                                       >  2:900967/1‑237 (MQ=255)
                                                                                                      cGGTTAAACGCCGTATCCAGCCTGGGGACAAGATGGCCGGTCGTCACGGAAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTAAGCCGGTAGACATCGTACTGAACCCGCTGGGCGTAACGTCTCGTATGAACATGGGACAAATCCTACGAAACCC‑CCCGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACCCCacaaagaaacac                                                                                                                                      >  2:624553/1‑228 (MQ=255)
                                                                                                       ggTTAAACGCCGTATCCAGCCTGGTGACAAGCTGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGACCCAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGTCGACCCGTCTCGTATGAACATCGGTCAGATCCT‑AGAAACCCACCTGGGTATGGCTCGGAAAGGGATCGTCGAGAAGATCaaagacacgccaaaaaaca                                                                                                                                     >  2:615281/1‑222 (MQ=255)
                                                                                                       ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                                                                                                                   <  1:1094446/209‑1 (MQ=255)
                                                                                                         ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCACGATCAACGCCACGCTGAAAcagcag                                                                                                                                   >  1:1043576/1‑239 (MQ=255)
                                                                                                                   tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAAATAAACCCCCAGCCGAAACACCAGCaaaaaaccg                                                                                                                         >  2:68691/1‑235 (MQ=255)
                                                                                                                     tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCACCCTGGGTATGGCTGCGAAAGGGATCGACGACAAGATCAACGCCATGCTGAAAAAGCAGCaagaagctcgg                                                                                                                       >  1:949925/1‑234 (MQ=255)
                                                                                                                         gCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTCGGCGTACCGTCTCGTATGAAACTCGGTCAGATCCT‑CGAAACCCACCTGGGTATGCTTGCGAAAGGGATCGGCGACAAAATAAAACCCCTCGTGAAACAGAAGCAAAAAGTCGCGaaa                                                                                                                    >  2:744902/1‑238 (MQ=255)
                                                                                                                          ccTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGCCAAGATCCACGCCAGCCTGAAACGGCACGCAGAAGTctcaaa                                                                                                                     >  1:714802/1‑231 (MQ=255)
                                                                                                                             ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg                                                                                                               >  1:777159/1‑239 (MQ=255)
                                                                                                                              gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATAGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGAATGGCTCCGAAAGGGATCGGCCACAAGATCAACGCCATACTTAAACAGCAGCAAGAAGTCCGGAAACGgcg                                                                                                               >  1:858858/1‑238 (MQ=255)
                                                                                                                                gaaaaGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg                                                                                                             <  1:931054/235‑1 (MQ=255)
                                                                                                                                gACAAGATGGCCGGTCGGCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg                                                                                                             <  1:561254/238‑1 (MQ=255)
                                                                                                                                        ggCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATcc                                                                                                    >  1:656342/1‑239 (MQ=255)
                                                                                                                                         gCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGAACAAGCAGCAAGAAGACGCGAAACTGCGCGAATTCATcc                                                                                                    >  1:69414/1‑238 (MQ=255)
                                                                                                                                          cAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCa                                                                                                   <  1:579939/238‑1 (MQ=255)
                                                                                                                                            ggTCTTCCCGGGAACAAGGGTTTAA‑TTTCTAAGATCAACCCGATCGAAGATAGGCCTTACGATGAAACCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcg                                                                                                <  2:609825/239‑1 (MQ=255)
                                                                                                                                             gtcgtcACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgc                                                                                               >  1:141599/1‑238 (MQ=255)
                                                                                                                                             gtcgtcACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAacgt                                                                                               >  1:142000/1‑239 (MQ=255)
                                                                                                                                              tcgtcACGGTAACAAGGGTTTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcg                                                                                                <  1:853860/237‑1 (MQ=255)
                                                                                                                                                gtcACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtgcg                                                                                            <  1:826911/239‑1 (MQ=255)
                                                                                                                                                gtcACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTAGCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtgcg                                                                                            <  1:21570/239‑1 (MQ=255)
                                                                                                                                                          cAAGGGTGTAA‑TTTCAAAGATCAACCCGATGGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgcgtgcg                                                                                            <  1:31928/229‑1 (MQ=255)
                                                                                                                                                               gtgtAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGAAGCAAGAAGTGGCGCAACTGCGCGAATTGATCCacggcacgaaggaactggggggg                                                                             >  2:941951/1‑217 (MQ=255)
                                                                                                                                                                 ttAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTa                                                                                          <  2:168621/223‑1 (MQ=255)
                                                                                                                                                                 gtAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCGGCGTGGGGACGATCTGGGCGCTGa                                                                           >  1:468705/1‑239 (MQ=255)
                                                                                                                                                                 gtAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTg                                                                            >  1:22698/1‑238 (MQ=255)
                                                                                                                                                                 gtAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTg                                                                            >  1:618502/1‑238 (MQ=255)
                                                                                                                                                                 gtAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGGCAAGATCAACGCCATGCTGAAACAGCCGCAACAAGTCGCGAAACAGCGCGAATTCATCCAGCGGGCGTACGATCTGGGGGCTgg                                                                           >  2:1042695/1‑238 (MQ=255)
                                                                                                                                                                 gtAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTACACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGGACGATCTGGGCGCTg                                                                            >  1:655901/1‑238 (MQ=255)
                                                                                                                                                                   aa‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGaaa                                                                                                                                                                                                 <  2:731534/119‑1 (MQ=255)
                                                                                                                                                                   aa‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGaaa                                                                                                                                                                                                 >  1:731534/1‑119 (MQ=255)
                                                                                                                                                                    a‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGGGACAAGATCACCGCCCTGCGGAAACCGCACCAAGAGATCGCGAAAATGAGCGAAATCATCCAGCCAGCCCACCAACTGGgcgagaccct                                                                        >  2:31928/1‑232 (MQ=255)
                                                                                                                                                                      tttCTTAGAACACTCCTATGGAAGATAGGCCTTCCGATGAAACCGGTACTCCGGTGGACAGCGTCCGGAACCAGCTGGGCGTACCGTCTCGTATGAAAATCGGTCAGATCCA‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGtt                                                                       <  2:933518/239‑1 (MQ=255)
                                                                                                                                                                      tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGGATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCCCGCGGAAACAGCAGCAAGAAGTCGGGAAACTGCGCGAATTCACCCAGCGGGCGTACGaatggggggcagagggt                                                                       >  2:269633/1‑223 (MQ=255)
                                                                                                                                                                                  aCCCGGTCGAAGATATGCCCTGCGATGAAAACGGTACGGCGGTAGACATCGTACTGAACCAGCTGGGCTTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCATCGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGtg                                                           <  1:745343/239‑2 (MQ=255)
                                                                                                                                                                                  aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGtt                                                           <  1:269633/239‑1 (MQ=255)
                                                                                                                                                                                   cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGACTTGGGCGCTGACGTTCGCCGGAAAGTTg                                                          >  1:692674/1‑239 (MQ=255)
                                                                                                                                                                                     ccaTCGAAGTTATGCCTTTCGGTGAAAACGGTACGGCGGTAGGCCTCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGAc                                                        <  2:52695/237‑1 (MQ=255)
                                                                                                                                                                                             gATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGAATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCCGCAAGTTGACCTGGGTa                                                 >  1:354410/1‑238 (MQ=255)
                                                                                                                                                                                                aagCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCtt                                             <  1:875677/237‑1 (MQ=255)
                                                                                                                                                                                                 tGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTc                                            >  1:121092/1‑239 (MQ=255)
                                                                                                                                                                                                  gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCCGAAAGTTGACCTGAGAACCTTc                                            >  1:395417/1‑238 (MQ=255)
                                                                                                                                                                                                  gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCa                                           >  1:203380/1‑239 (MQ=255)
                                                                                                                                                                                                  gCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCCTGCTGAAACAGCAGCAAGAAAGCGCGAAACTGGGCGAATTCATCCAGCGTGGGTACGATCTGGGCGCCGACGTTCGGCCAAAAGTTTAcccaaagaccgccc                                           >  2:491697/1‑227 (MQ=255)
                                                                                                                                                                                                    cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAg                                          <  1:916346/238‑1 (MQ=255)
                                                                                                                                                                                                       aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCTTGCGTACGACCTGGGCGCTGGCGTTCGCCAGCAAGTTAACCGTAGTCCCTTCAGCGac                                      >  1:423210/1‑238 (MQ=255)
                                                                                                                                                                                                       aCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAATGCGCGAATTCATCCAGCGGGCGGACGCTCTGGGCCATGAAGTTCCTCCGAAAGTTGACCGGGAGACCTTAAGCGaa                                      >  2:897007/1‑238 (MQ=255)
                                                                                                                                                                                                        cGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATg                                     <  1:636208/239‑1 (MQ=255)
                                                                                                                                                                                                        cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATg                                     <  1:897007/239‑1 (MQ=255)
                                                                                                                                                                                                            taaacCGGTACCGCGGTGAACATCGTACTGAACCAGCGGGGCTGACCGTCTCGTATGAACTTCGGTCAGATCCC‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATgaag                                  <  2:1013945/237‑1 (MQ=255)
                                                                                                                                                                                                                aCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGtt                             <  1:1053979/239‑1 (MQ=255)
                                                                                                                                                                                                                      cGCCGGTAGAACTGGTACGAAACCAGCTGGGGGTACCGTCTCGTATGAACAGCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGt                       <  1:458901/239‑1 (MQ=255)
                                                                                                                                                                                                                            tAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGCAACTGCGCGAATTCATCCCGCGTGCGTACGACCTGGGCGCTGACGTTCGTCAGAAAGTTGACCCGAGTCCCTTCAGCGGTGAAGAAGGTATGGGTccgtct                 >  2:201573/1‑234 (MQ=255)
                                                                                                                                                                                                                               aCATCCTATTGAACCAGTCGGGTGTACGGTCTCGTATGAAAATCGGTCAGATCCA‑CGAAACCCGCCTGGGTATGGCAGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGaa              <  2:69414/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tccttcTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAAc           <  2:409592/234‑1 (MQ=255)
                                                                                                                                                                                                                                  tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAAc           <  1:201573/239‑1 (MQ=255)
                                                                                                                                                                                                                                   cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAg                                          <  1:615635/207‑1 (MQ=255)
                                                                                                                                                                                                                                   cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAg                                          >  2:615635/1‑207 (MQ=255)
                                                                                                                                                                                                                                   cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGTTGAAGAAGTTATCCGTCTGGCTGAAAAcc          >  1:8877/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGaaaa            >  1:415924/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGGGCGTACGAACTGGGGGCTGACGTTCGTCAGAAAGTTGACCTGAGTCCCTTCCGCGATGAAGAAGTTATggcgcgggggaaaaaac          >  2:920134/1‑223 (MQ=255)
                                                                                                                                                                                                                                           aCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCaaa  <  1:1042695/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
TGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAA‑TTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCT‑CGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGCGTGCGTACGATCTGGGCGCTGACGTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCAAA  >  NC_000913/4184305‑4184777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A