Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F107 I2 R1	9	28.1	973420	94.3%	917935	142.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,184,542	C→A	P1100Q (CCG→CAG)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,184,542	0	C	A	97.7%	121.9 / NA	44	P1100Q (CCG→CAG)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (27/16); minor base G (0/1); total (27/17)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  >  NC_000913/4184405‑4184681

ggCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTATCAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                        >  2:145579/1‑143 (MQ=255)

   ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGACAAGAAAACATGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                     >  1:332692/1‑143 (MQ=255)

      tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                  >  1:471918/1‑143 (MQ=255)

      tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATGTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGtgc                                                                                                                                  >  1:438960/1‑141 (MQ=255)

         aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcccg                                                                                                                               >  2:181834/1‑141 (MQ=255)

          cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTCACCAGGGTGTAATTTCTAAGATCAACCCGATCGACGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtc                                                                                                                              >  2:148008/1‑143 (MQ=255)

          cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtc                                                                                                                              >  2:53766/1‑143 (MQ=255)

            ccGTAACGAGCCTGGGGATAAGATGGGAGGGCGACACGGGAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                            <  1:104736/143‑1 (MQ=255)

            acGTATTCAGCCTCGTCATAATATGTCAGGTCTTCACTGTCACAATGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATCAATTCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                            <  1:141188/142‑1 (MQ=255)

                         ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACCTCGTACTGAACCAGCTCGGCGTACCGTCCCGTATGCACATCgg                                                                                                               >  2:163804/1‑143 (MQ=255)

                         ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCgg                                                                                                               <  2:434589/143‑1 (MQ=255)

                           tggcAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                             <  2:370325/140‑1 (MQ=255)

                           tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTAATGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                             >  2:159008/1‑143 (MQ=255)

                               aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGac                                                                                                         >  2:47948/1‑142 (MQ=255)

                               aaGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                         >  2:224366/1‑143 (MQ=255)

                                      cAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATAGGtaggacacgccaa                                                                                                  >  2:187601/1‑131 (MQ=255)

                                       aGGTCGTCAACGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                 <  1:429900/143‑1 (MQ=255)

                                          tcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATGCGTCAGATCCTCGAAAccc                                                                                              >  2:389949/1‑143 (MQ=255)

                                             tcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCGTAAGATGAGAGCGGTACGCCGGTAGGCATCGTGCTGAACCAGCTGGGCGTACCGTCTCGTATGACCATCGGTCAGAGCGTGGACACACAcc                                                                                           >  1:252488/1‑143 (MQ=255)

                                                              tagtttCGAAGGTCAACGCGATCGAAGATATGGCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                          <  1:196420/140‑1 (MQ=255)

                                                               aagttCGAAGATCAACCCGATCGAAGAGAGGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                         <  1:287334/140‑1 (MQ=255)

                                                               aaTTTCTAAGATCAATCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                         >  2:213927/1‑143 (MQ=255)

                                                               aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAATGTGACGTCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGCATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                         <  1:338303/143‑1 (MQ=255)

                                                                aTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGGATGGCTGCGGAAga                                                                        >  2:124187/1‑142 (MQ=255)

                                                                   tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCCGGGCGTCCCGTCTCGTATGAACATCGGTCAGATCCACGAAACCGACCTGGCCAGGGCTGCGaatgccac                                                                     >  2:138674/1‑137 (MQ=255)

                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCg                                                                   >  1:17256/1‑143 (MQ=255)

                                                                              cccGATCGAAGATATGCCTTACGATGAAAAAGGTACGCCGATAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGa                                                          >  1:92553/1‑143 (MQ=255)

                                                                                 gATCGAAGATATGCCTTACGATGAAAACGGTCCGCCGTCAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGACAGGCATACGCGGCAAGATCa                                                       >  2:149376/1‑143 (MQ=255)

                                                                                  aTCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGCTCaa                                                      >  1:92997/1‑143 (MQ=255)

                                                                                    cGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAGGGCTGCGCAAGGGATCGGCGACCAGATCAACg                                                    >  2:449693/1‑143 (MQ=255)

                                                                                          tatGACTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                              <  2:398992/143‑1 (MQ=255)

                                                                                               cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                         <  1:224366/143‑1 (MQ=255)

                                                                                                ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAc                                        >  2:19355/1‑143 (MQ=255)

                                                                                                   cGATGAAAACGGTACGCCGGTAGACATCGGACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                     <  2:200207/143‑1 (MQ=255)

                                                                                                      tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACagcagc                                  <  1:398516/143‑1 (MQ=255)

                                                                                                       gAAATCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAACGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                                 >  2:197815/1‑143 (MQ=255)

                                                                                                       gAAAACGGTACGCCGGTAGACATCGTACTGAACCGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAgcagca                                 <  2:197852/143‑1 (MQ=255)

                                                                                                        aaaaCGGTACGCCGGGAGACATCGTGCTGAACCAGCTGGGCGTACCGTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaa                                <  1:163804/143‑1 (MQ=255)

                                                                                                                  gCCGGTAGACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGa                      <  1:84278/143‑1 (MQ=255)

                                                                                                                          aCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcgc              <  1:159008/143‑1 (MQ=255)

                                                                                                                              cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt          <  1:419050/143‑1 (MQ=255)

                                                                                                                               gTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGCGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaaat         >  2:275787/1‑141 (MQ=255)

                                                                                                                                 aCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCa       >  1:400343/1‑143 (MQ=255)

                                                                                                                                      aCCAGCTGGGCGTACCGTCTCGTATGAACATCGATCAGATACTCGAAACCCACCTAGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAAGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTACGCGAATACAACCac  >  2:15966/1‑142 (MQ=255)

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAG  >  NC_000913/4184405‑4184681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A