Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA100.0% 495.5 / ‑6.7 148P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (72/72);  total (73/75)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 148 total aligned reads displayed.

ACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCA  >  NC_000913/4184309‑4184775
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
acacGGGTTCGAGAAGAAACGCGACGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                                                                                                                        <  1:202167/239‑1 (MQ=255)
      gTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑GACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                                                                                                                   <  2:612271/238‑1 (MQ=255)
        tcgggaagacatcgtaatggAAACGGCGAAAAACCCCCCAGGGCGCTGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGGGACAAGATGGCAGGTGGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTa                                                                                                                                                                                                                                   <  1:25808/217‑1 (MQ=255)
         catgaaaaaccgcgagtgcaaaCGCCGCAAAATCACCCAGGGTGTCGATCTGGCACCGGGCGTGCTGAAGATTGTTAGGGGATACCAGGCGGTTAAACGCCGTAGCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGTGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCT‑TACGATGAAAACGGTACGCCGGTAGACAACGTACTGAACCATCTGTGCGTACCGt                                                                                                                                                                                                                               <  1:209844/220‑1 (MQ=255)
         cGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                                                                                                                               <  1:139219/239‑1 (MQ=255)
                     cGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACa                                                                                                                                                                                                                   <  2:1137020/239‑1 (MQ=255)
                           gAAACGCCCCAAAATCCCCCAGGGCGACGTTTTGGCCCCGGGCGTGCTGAAGATTGTTAAGGTATAGCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGt                                                                                                                                                                                                              <  1:29726/238‑1 (MQ=255)
                              acgacgCAAAATCACCCAGGGCGACGATCTGGCCCCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGa                                                                                                                                                                                                          <  2:220864/239‑1 (MQ=255)
                               cgccgcAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                                                                                                                         <  2:1213543/239‑1 (MQ=255)
                               ccccgcAAAATCCCCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGAt                                                                                                                                                                                                         <  2:698773/237‑1 (MQ=255)
                                    caaatacACCCAGGGCGGCCGCCTGGCCCCAGGTGGGCTGGAGGTTGTTAATGTATACCTGGCGGTTAAACCCCGTGTCCAGCCGGGTGACAAGATGGCAGTGCGT‑CACGGTAACAAGGGTGAAATTGCTAAGATCAACCCGAGCGAAGAGATGCCT‑TACGATGAAAACGGTACGCCGGTAGACGTCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTAAGATACTCg                                                                                                                                                                                                    <  1:1211491/233‑1 (MQ=255)
                                       aaccccccAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                                                                                                                 <  2:497080/234‑1 (MQ=255)
                                            cccGGGGGGACCGTCGGGCCCCGGGGGTGCTGAAGGTTGTTAAGGGAGTTCGGGCGGTTAAATGGCGTTGCCAGCCTGTGGACAAGATGGCAGGTCGT‑ACCGGTAACAAGGGTGGAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTAAGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                                                                                                                            <  1:930990/239‑1 (MQ=255)
                                            cccAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                                                                                                                            <  2:454183/239‑1 (MQ=255)
                                            cccAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCGGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGAGCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                                                                                                                            <  2:429507/239‑1 (MQ=255)
                                                     acgaTCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGa                                                                                                                                                                                                          <  2:198842/216‑1 (MQ=255)
                                                     acgaTCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAAACCGATCGAAGATATGCCT‑TACGATGAAAACGGAACGCCGGTAGACAATGGACTGACCCAGCTGGGCGTACCGTCTTGATCGAAAAACGGTCAGACCCTCCAAACCCCCCTGGGGATg                                                                                                                                                                                   >  1:825780/1‑239 (MQ=255)
                                                                   ggCGTGCTGAGGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGAGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGAGGAAAACGGTACGCCGGTAGACACCGTACTGAACCCGATGGGCGGACCGTCTCGGATGAACATCGGTCAAACTCTCGAAACCCACCCGGGTACGGAATCGAAAGGGAt                                                                                                                                                                     >  1:1016887/1‑239 (MQ=255)
                                                                   ggCGTGCTGACGATTGTTAAGGTATATCTGGCGGTTACACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTAGT‑CACGGTAACAAGGGTGTACTTTCTAAGATCAACACGATCGACGATATGCCT‑TACGATGGAAACGGTACGCCGGTAGACATAGTCTTGACCAAACTAGGCGAACCGTGTCCCACGACCATCGGTCCGATACTCGCAACACGACCGGGGATGGCTGGGAAAggaac                                                                                                                                                                     >  1:1001152/1‑236 (MQ=255)
                                                                   ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATTAAAACGGTACGCCGGTAGACATCGAACTGAACCAGCTGGGCGTACCGTCTCGCATGACAATCGGTCAGCTCCTCGAAACCCACCTGGGTATGGCACCGAAAGGTAt                                                                                                                                                                     >  1:1199693/1‑239 (MQ=255)
                                                                   ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGGGTACCGTACCGTATGAACATCGGGAAGATCCTCTAAACCCACCTGGGAATGGCTGGGAAAGGCAt                                                                                                                                                                     >  2:1223073/1‑239 (MQ=255)
                                                                   ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAggga                                                                                                                                                                      >  2:849176/1‑236 (MQ=255)
                                                                   ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGCAGACATCGTACTGAACCAGCTGGGCGAACCGTCTCGTATGAACATCGGTCAGATCCCCGAAACCCACCTCGGTATGGCTGCGCAAGGGAt                                                                                                                                                                     >  1:841677/1‑239 (MQ=255)
                                                                   ggCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGCGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGGGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCCCGTATGAACATCGGTCAGATCCTCGGAACCCACCTGGGGATGGCTGCGAAAggaa                                                                                                                                                                      >  2:142169/1‑236 (MQ=255)
                                                                     cGTTCTGAAGTTTGTTAAGGTTTTTCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATc                                                                                                                                                                    <  1:304472/238‑1 (MQ=255)
                                                                          tggataaaataaggggtcattttgCGGATAAACGCCGTGCGCAGCCTGGTGACAAGAGGGCAGGTGGT‑CACGGTAAAAAGGGTAGAAGTTCAAAGATCAACCCGCACGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                                                                                                                              <  1:284209/216‑1 (MQ=255)
                                                                           gAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGCTCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTGGAAAACCACCTGGGAATGGCTGCGGAAGGAATCGGCGACa                                                                                                                                                             >  2:1012579/1‑239 (MQ=255)
                                                                                     aaGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                                                                                                                   <  2:850864/239‑1 (MQ=255)
                                                                                     aaGGTATATCTGGCGGTTAAACGCCGTAACCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGc                                                                                                                                                   <  2:616595/239‑1 (MQ=255)
                                                                                                  cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACACAATCAACGCCATGCTGAAAca                                                                                                                                       >  2:1155461/1‑238 (MQ=255)
                                                                                                  cGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCT‑GGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTCGGTATGGCTGGGAAAGGAATCGGCGACAAGATAAACACCAAGCGGAAAaaga                                                                                                                                     >  1:447203/1‑238 (MQ=255)
                                                                                                   ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATGGGCGCCAAAATCAACCCCATGCGTAAAcaga                                                                                                                                     >  2:443288/1‑238 (MQ=255)
                                                                                                   ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAAATCCTCGACACCCACCTGGGTATGGCTGCGAAAGGCATCGGCGACAAAATCAACGcttgtcccttttac                                                                                                                                      >  2:284209/1‑225 (MQ=255)
                                                                                                   cgTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcag                                                                                                                                      <  2:575788/237‑1 (MQ=255)
                                                                                                     ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                                                                                                                   >  2:165213/1‑239 (MQ=255)
                                                                                                       aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                                                                                                                 <  1:336910/175‑1 (MQ=255)
                                                                                                       aaaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                                                                                                                 >  2:336910/1‑175 (MQ=255)
                                                                                                        aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAAAGCAGCaa                                                                                                                                >  2:133133/1‑239 (MQ=255)
                                                                                                          cccctgagcagtccTGGTGAGCAGATGGGAGGATGTCCACGG‑AACAAGGGTTGAATTTCTAAAGTCACCCCGACCGAAGATACGCCC‑TACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTATCGTCGCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaaga                                                                                                                              <  1:671911/227‑1 (MQ=255)
                                                                                                             gggttCCAGCGCGGTGACAAGAGGGCAGGGCGT‑CACGGAAACAAGGGTGTAATTTCTAAGATGACACCGATCGAAGATAAGCCT‑TACGATGAAAACGGTACGCGGGTAGACATCGTACTGAACCAGCAGGGCGTACCGTCTCGTAGGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAGAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaagaag                                                                                                                            <  1:1301197/234‑1 (MQ=255)
                                                                                                               tATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                                                                                                                          >  2:116755/1‑238 (MQ=255)
                                                                                                               tATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCCTGCTGAAACAGCAGCAAGAAGTc                                                                                                                          >  2:338788/1‑238 (MQ=255)
                                                                                                               tATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACACGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                                                                                                                          >  2:1151787/1‑238 (MQ=255)
                                                                                                                       cTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACt                                                                                                                  >  2:436919/1‑238 (MQ=255)
                                                                                                                         ggTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGCATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAACAGCAAGAAGTCGCaaaaacacg                                                                                                               >  1:186866/1‑234 (MQ=255)
                                                                                                                         ggTGAAAAGATGGCACGGTGT‑GACCGGAAAAAGGGTGTAATGTCTAAGATCAACCCGATCGGAGATATGCCT‑TACGATGAAAACGGTCAGTCGGTAGGCACTGTACTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg                                                                                                               <  1:851020/239‑1 (MQ=255)
                                                                                                                            gACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGcgcg                                                                                                             <  2:917919/238‑1 (MQ=255)
                                                                                                                                aGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCGGCGAAAGGTATCGGCGACCAGCTAAACCCCATCCTGGAAACGCAGCAAGAAACCGCGAAAATgccgcaatta                                                                                                        >  1:1230939/1‑231 (MQ=255)
                                                                                                                                   tGGCAGGTCGT‑CGCGGGAACAAGGGTGTAATTTCGTAGCTCAAACCGAACGAAGATAGGCCT‑TACGATGAAAAGGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc                                                                                                     <  1:248378/239‑1 (MQ=255)
                                                                                                                                    ggCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTCCGCGAATTCATc‑c                                                                                                   >  2:662500/1‑239 (MQ=255)
                                                                                                                                    ggCAGGGCTT‑CACGGTCACAAGGGTGGAATTTCGACGGATAACCCCATCGAAG‑GATTCCTGGTCTGGTAAAACGGTTCGCCGGTAGACATCGTACTGAACCAGGTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCGAGAAACCCACCTGGGTATGGCTGCTAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATc‑c                                                                                                   <  1:744011/239‑1 (MQ=255)
                                                                                                                                         gttgtgcACGGGAATAGGGGTGTAAATTAGAAGTTCAACCCGAACGAAGATTTGCCT‑TGCGATGCAAACGGTAAGCCGGTAGACATCGTACGGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCCGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAgcg                                                                                               <  1:991290/233‑1 (MQ=255)
                                                                                                                                           cgt‑cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATTGGCGACAAGATCAACGCCAGTCTGAAACAGCAGCAAGAATTCGCGAAACTGCGCGCATTCATC‑CAgcgtcc                                                                                            >  2:248378/1‑237 (MQ=255)
                                                                                                                                                      aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCt                                                                                   <  2:468120/238‑1 (MQ=255)
                                                                                                                                                            gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGgcgcg                                                                            >  2:207296/1‑238 (MQ=255)
                                                                                                                                                            gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGCATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCCCGAAACTGCGCGAATTCATC‑CAGCGTCCGTACGATCTGGgcgcg                                                                            >  2:647330/1‑238 (MQ=255)
                                                                                                                                                             tgtAATCTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACACACCTGGGTATGGCTGCGAAAGGCACCGGCGCCAAGATCAACCCCCTGCTGAAAACGCCGAAAGAAGTCCCGAAACGCCGCCAATTCATC‑CAgccgcgcaacagcaagggccggg                                                                           >  1:760780/1‑218 (MQ=255)
                                                                                                                                                              gtAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTg                                                                           >  2:909375/1‑238 (MQ=255)
                                                                                                                                                               tAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                                                                                                                      <  2:217345/131‑1 (MQ=255)
                                                                                                                                                                     cTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCATCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCCGCAAAAGATCGCGAAACTGCCCGAATTCATC‑CAGCGTGCGGACCACTTGGGCGCCGCC‑GTTcc                                                                   >  1:469094/1‑237 (MQ=255)
                                                                                                                                                                     cTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGAAACGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGGCAAAATGCCGGAATTCATC‑CAGCGTGCGTACGATCCGGGCGCTGAG‑GTACgc                                                                  >  1:913359/1‑238 (MQ=255)
                                                                                                                                                                             aaCCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCAACCTGGGTATGGCTGCGAAAGGTATCGGAGACACGATCAACCCCATGCTGAAACAGCAGCAAGAAAGCGGGAAACTGAGCGACTTAATCACAGC‑TGCGTAAGATCCGGGCGCTGCC‑GTGCggacacaagg                                                          >  1:863798/1‑230 (MQ=255)
                                                                                                                                                                              aacggATCGAAGATATGCAT‑TACGAAGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGtt                                                         <  2:1052281/235‑1 (MQ=255)
                                                                                                                                                                              aCCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAGGCCATGCTGAAAAAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGAGCGTACGATCTGGGCGCTGCC‑GTTCGACAGGAAGtt                                                         >  2:104552/1‑239 (MQ=255)
                                                                                                                                                                              aCCCGAACGAAGAAATGCCT‑TAAGATGAACACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAAATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGtt                                                         <  2:128643/239‑1 (MQ=255)
                                                                                                                                                                                 cGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCT‑GGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGAcc                                                     <  2:447203/239‑1 (MQ=255)
                                                                                                                                                                                  gATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGAcc                                                     <  2:841677/239‑1 (MQ=255)
                                                                                                                                                                                    tCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAACAGCAAGAAGGTGGCAAACTGTGCGAATTCATC‑CAGCGTGCGTACCGTCTGGGCGCTGCC‑GACCCTCACAAAGTTGAccgc                                                   >  1:597098/1‑237 (MQ=255)
                                                                                                                                                                                    tCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGTGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTg                                                   >  2:132105/1‑239 (MQ=255)
                                                                                                                                                                                       aaGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACCGCGCGAATTCATC‑AAGCGTGCGCACGCGCGGGGCGCGGAC‑GTTCGCAAGAAAAttacaccggga                                                >  1:466393/1‑230 (MQ=255)
                                                                                                                                                                                        aGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAGACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTgg                                                                                 <  2:140582/206‑1 (MQ=255)
                                                                                                                                                                                        aGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGt                                                <  2:900136/238‑1 (MQ=255)
                                                                                                                                                                                              gCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                                                                                                                               <  1:445001/91‑1 (MQ=255)
                                                                                                                                                                                              gCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGCAGCGGCCACAAGATCAACGCCATGCTCAACCAGCAGCAAGAAGTCGGGAAACTCCGCGAATTCCTC‑CAGCGGCCGCACGAGCTGGGCGCTGAC‑ATTCCACCGAAAGTTGACCCGAAGACATTCa                                         >  1:33305/1‑239 (MQ=255)
                                                                                                                                                                                               ccT‑TTCGGTGAAAACGGTCCCCCGGTAGACATCGTTCTGAACCAGCTGGGCGTACCGGCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCTACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTCCCTTCAg                                        <  1:1231818/239‑1 (MQ=255)
                                                                                                                                                                                               ccT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGGATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGCGAAACTGCGCGAATTCATC‑CAGCGTGGGGACGACCTGGGGGCTGAC‑GTTCGGCAGAAAGTTGACCTGAGAACCTTCAg                                        >  1:570424/1‑239 (MQ=255)
                                                                                                                                                                                                cctttgcactaatacggtcggggggTTGACACAGTTCTGAACCCAGTGGGCGTACCTGTTTGTAAGGACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTACCGGCAATAAGATCAACGACATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCGGAGTACCTTGAg                                        <  1:880764/216‑1 (MQ=255)
                                                                                                                                                                                                cT‑TACGATTAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAg                                        <  2:1015579/238‑1 (MQ=255)
                                                                                                                                                                                                cT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAg                                        <  2:1230939/238‑1 (MQ=255)
                                                                                                                                                                                                   tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAACCAGCAGCAAGAAGTCGCGAAACTGCGCGTATTCATC‑CAGCTGGCGGACGA‑CTCGGCGGCGTCGGTTCGTCAGACATGTGATCTGCGCCACTTCAGCGa                                     >  1:1148213/1‑239 (MQ=255)
                                                                                                                                                                                                   tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCCTC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGCCCTGAGTACCTTCcgcgc                                     >  2:84583/1‑238 (MQ=255)
                                                                                                                                                                                                   tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGa                                     >  2:1278059/1‑239 (MQ=255)
                                                                                                                                                                                                   tACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTAGCGGCGACAAGATCAACCCCATGCTGAAACATCAGCCAGAAGCAGCGACACTGCGCGAATTCAGC‑CAGCGTGCGTACGATCTGGGGGCGGAC‑GTGCGTCCGAAAGGTGACCTGAg                                                 >  1:1279315/1‑227 (MQ=255)
                                                                                                                                                                                                     cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGCCAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGGGCGTACGATCTGGGGGCTACC‑GTTCGTCGGAAAGTTGACCTGAGTACCTTCAGCGATg                                   >  2:788470/1‑239 (MQ=255)
                                                                                                                                                                                                     aatggAAAACGGTCACCCGGTAGACATTGTACTGAACCAGCGGGGCGTACCTTCCCCTAGGAACATCGGTCAGATCCCCGAACCCCACCTGGGTATGGCTGCGAAAGGTAACGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATg                                   <  1:1278059/235‑1 (MQ=255)
                                                                                                                                                                                                        tGAAAACGGTACGGCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATgaa                                 <  2:1046624/238‑1 (MQ=255)
                                                                                                                                                                                                        tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAAGCCCTGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CCGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAATTGACGTAAGTACCTTCACCGagaagg                                >  1:188581/1‑234 (MQ=255)
                                                                                                                                                                                                        tGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATAGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGGGCGTACGATCTGGGGGCTGAC‑GTTCGCAAGGAGATTAACCTACGAACCATCAGCGAGgaaa                                >  1:1135838/1‑238 (MQ=255)
                                                                                                                                                                                                          aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGGGTTgaggaa                              >  2:1256981/1‑239 (MQ=255)
                                                                                                                                                                                                          aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTAAGCGATgaagaa                              >  2:802032/1‑239 (MQ=255)
                                                                                                                                                                                                          aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGCCGACAAGATCAACGCCATGATGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGAACTGGGCGCTAGC‑GTTCGGCAGAAAGTTGCACTTGGTAACTTCAGCGCTgacaga                              >  1:1015579/1‑235 (MQ=255)
                                                                                                                                                                                                                 tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGCAGAAGTTATGc                       >  2:1279700/1‑239 (MQ=255)
                                                                                                                                                                                                                         tAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCACCAAGAAGTCCCGAAACTGCGCGAATTCATC‑CAACCGACGTACCACCTGGGCGCTGAC‑GTTCGTCAGAAAGATTACCTCAGTACCTTCAGCGTTGAAAAAATAATgagcggcgcg               >  1:283983/1‑230 (MQ=255)
                                                                                                                                                                                                                               tcttacgggaCCAGCTGGGGGTACCGGTTCGTATTAAAAGCGGTCAGAACCCCGAAACCCCCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAACAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAAc         <  1:436919/230‑1 (MQ=255)
                                                                                                                                                                                                                               tCGTACTGAACCAGCTGGGGGTACCGTCTCGTATGACACTCGGTCAGATCCCCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGGCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAAc         <  1:662500/239‑1 (MQ=255)
                                                                                                                                                                                                                                cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCTTC‑CAGCGTGCGTACGAGCTGGGCGCTGAC‑GTTCGTCCGAAAGTTGACCTGAGTACCTTCAGGGTTGCAGAAGTTATGGGTCTGGCTGAAAAcc        >  2:649021/1‑239 (MQ=255)
                                                                                                                                                                                                                                cGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGCCTGGCTGaaaa          >  2:923903/1‑237 (MQ=255)
                                                                                                                                                                                                                                    cTGAACCAGGTTGGCCTACCGTCTCGTAAGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTgcg    <  2:1225952/239‑1 (MQ=255)
                                                                                                                                                                                                                                    cTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTgcg    <  2:69097/239‑1 (MQ=255)
                                                                                                                                                                                                                                      gAACCAGCGGGGCGTCCCGTCTCGTATGAACATCGGTCAGATCCTCTAAACCCTCCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGGCAGAAAGTTGACCTGAGTACCTTCAGCGCTGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCa  <  2:152873/239‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
ACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCGACGATCTGGCACCGGGCGTGCTGAAGATTGTTAAGGTATATCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGT‑CACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCT‑TACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC‑CAGCGTGCGTACGATCTGGGCGCTGAC‑GTTCGTCAGAAAGTTGACCTGAGTACCTTCAGCGATGAAGAAGTTATGCGTCTGGCTGAAAACCTGCGCA  >  NC_000913/4184309‑4184775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A