Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F60 I1 R1
|
12 |
40.2 |
1378958 |
98.9% |
1363789 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,815,883 |
+C |
coding (667/687 nt) |
rph ← |
truncated RNase PH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,815,879 | 1 | . | C | 82.1%
| 84.0
/ 11.1
| 29 | coding (671/687 nt) | rph | truncated RNase PH |
| Reads supporting (aligned to +/- strand): ref base . (4/1); new base C (10/13); total (15/14) |
| Fisher's exact test for biased strand distribution p-value = 3.26e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC > NC_000913/3815741‑3816012
|
cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga‑t‑t‑c < 2:40633/140‑1 (MQ=255)
cAATAAACTGGCGCTAATATGGCTTGATGGCTTCGCTACTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga‑t‑t‑c < 1:329102/140‑1 (MQ=255)
cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGcc < 2:509148/140‑1 (MQ=255)
cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTGCTGCGTCGCTACAAGGGATTCGA‑T‑TCC‑CCCTCGGGcc > 1:203677/1‑140 (MQ=255)
gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCagag < 1:400588/140‑1 (MQ=255)
ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGCCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg < 2:398281/140‑1 (MQ=255)
ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg < 1:112088/140‑1 (MQ=255)
ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg < 1:348966/140‑1 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATg > 2:571720/1‑140 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CACCCTCGGGCCAGAGCCAACAAGATg > 1:253701/1‑140 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAAGGATTCGG‑T‑T‑CTCCATCGGGCCAGAGCCAACAAGATg > 1:232994/1‑140 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg > 2:365105/1‑136 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg < 1:365105/136‑1 (MQ=255)
ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAg < 1:552464/140‑1 (MQ=255)
gacagaTCTTACTTTTCTACAGTCAAAAAAAGGGCAACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt < 2:250660/135‑1 (MQ=255)
ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑CTCCCTCGGACCAAAGACAACAACATGAGTAGCTCTTCa > 1:205815/1‑140 (MQ=255)
tcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt < 2:253701/140‑1 (MQ=255)
cTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TCC‑C‑CCCTCGGGCCTGAGCCAACAAGAGGAGTAGCTCTTCATGGGTGAAc > 2:294021/1‑140 (MQ=255)
ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TAC‑CCCTCGGCCCATAGCAAACAAGATGAGTAACTCTTCATGGGTGAACg > 2:179536/1‑140 (MQ=255)
cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAACCAACAAGATGAGTAGCTCTTGATGGGTGAAcctgtc > 1:250660/1‑135 (MQ=255)
cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc > 1:363534/1‑140 (MQ=255)
cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc < 2:115381/140‑1 (MQ=255)
aaaaaaGGCGACTCACCAGTCGGCTGAAAAATCAGTTCGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc < 1:294021/140‑1 (MQ=255)
aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCATCATGGGGGAATGGATCGCCTGCTGCCGTCCCCTGCa > 1:212180/1‑140 (MQ=255)
gCCTTAAAAATCAGTTTCCCAGCGCCGCCTTATGCCTCGCTACAATGGCTTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg < 2:194301/140‑1 (MQ=255)
aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc > 2:586794/1‑140 (MQ=255)
gCCAGCGCCGCCTTCCGCGTCGCTACAATCGATTCGACT‑C‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat < 1:179536/140‑1 (MQ=255)
gccgccTTCTGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTTTGCCGTCCCCTGTACTGCAATGATGAGCCCTGCTTCGGTGATCACTAc > 2:318407/1‑140 (MQ=255)
tGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca < 1:603989/127‑1 (MQ=255)
tGCGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca > 2:603989/1‑127 (MQ=255)
cGTCGCTACAATGGATTCGA‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCGTCAGTCATCGCTACGTTCATGTCgg > 1:207360/1‑140 (MQ=255)
gattcga‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga < 1:571720/140‑1 (MQ=255)
gattcga‑t‑tcC‑CCCTCGGGCCACAGTCAAAAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga < 1:318407/140‑1 (MQ=255)
ttcga‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCTGTCATCAATACGTTCATGTCGATCTCTGCGGCAGAGt > 1:289052/1‑140 (MQ=255)
tcga‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCagaggc > 2:455942/1‑138 (MQ=255)
|
CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑T‑T‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC > NC_000913/3815741‑3816012
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
