Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A45 F60 I1 R1	13	131.6	4491606	98.7%	4433215	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	97.0%	242.9 / ‑0.4	67	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (30/35); total (32/35)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑C‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815746‑3816012

aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑c‑cccct                                                                                                                                  <  1:527942/140‑3 (MQ=255)

  cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGACGCATTCTGCGTCGCTTCAATTGATTCG‑ATT‑c‑cccctcg                                                                                                                                >  2:622217/1‑136 (MQ=255)

  cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACACATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑c‑cccctcg                                                                                                                                >  1:1851925/1‑136 (MQ=255)

   tGGCGCTGATTTGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑c‑cccctcgg                                                                                                                               >  2:1905178/1‑135 (MQ=255)

     gcgcTGATATGGTTTCATGCCTGCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGc                                                                                                                             <  1:1090112/140‑1 (MQ=255)

      cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCAGCGGCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGcc                                                                                                                            >  2:813237/1‑140 (MQ=255)

       gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCa                                                                                                                           <  1:1494195/140‑1 (MQ=255)

        cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCag                                                                                                                          <  2:2034312/140‑1 (MQ=255)

        cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCGGCGTCGCGACAGTGGATTCGAATC‑C‑CCC‑TCGGGCCag                                                                                                                          >  1:572675/1‑140 (MQ=255)

          gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCagag                                                                                                                        <  1:361183/140‑1 (MQ=255)

          gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCagag                                                                                                                        >  1:2105446/1‑140 (MQ=255)

                  ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAg                                                                                                                <  2:2192586/140‑1 (MQ=255)

                   tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGa                                                                                                               >  1:908500/1‑140 (MQ=255)

                    cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGAt                                                                                                              >  2:148019/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCca                                                                                                                     >  1:1915846/1‑132 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCca                                                                                                                     <  2:1915846/132‑1 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  2:1159747/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:1425602/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGAGTCGCTACGATGGATTGG‑ATT‑C‑CCCATCGGACCAGAGCCAACAAGATg                                                                                                             >  2:595160/1‑140 (MQ=255)

                      tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGa                                                                                                            >  2:1774415/1‑140 (MQ=255)

                          ttCGCTCCTCATCTTTCTTTTCTACTGACTAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:882335/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTTCTTTTCTACCGACCAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:595160/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:1774415/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:1425602/140‑1 (MQ=255)

                            cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGct                                                                                                      >  1:2217773/1‑140 (MQ=255)

                                         aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:243767/125‑1 (MQ=255)

                                         aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        >  1:243767/1‑125 (MQ=255)

                                         aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCAAGACCCAACAAGATGAGCAGCTCTTCATGGGTGaa                                                                                         >  2:858739/1‑140 (MQ=255)

                                          cTTTTCTACAGACAACAAAAAGTCGTCTCTTCAGTCGGCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                                                                        <  1:1047438/140‑1 (MQ=255)

                                               cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                                                                   <  1:567720/140‑1 (MQ=255)

                                               cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                                                                   >  2:1598190/1‑140 (MQ=255)

                                                     aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                             >  2:1549673/1‑140 (MQ=255)

                                                     aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                             >  2:214585/1‑140 (MQ=255)

                                                     aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                             >  1:1190628/1‑140 (MQ=255)

                                                         aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                         <  1:148019/140‑1 (MQ=255)

                                                            aaaGGCGACTCATCAGAGGCCTTAAAAATCGGTTTCCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                      <  2:1039600/140‑1 (MQ=255)

                                                              aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    >  2:1885680/1‑140 (MQ=255)

                                                               ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAAGAAGATGTGCAGCGCTTGATGGGTGAGCGGCGCGCCTTCTGCCGTcccc                                                                   >  2:599978/1‑140 (MQ=255)

                                                               ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  1:1224587/1‑140 (MQ=255)

                                                               ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  1:1122825/1‑140 (MQ=255)

                                                                 cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:908500/140‑1 (MQ=255)

                                                                 cGACTCATCAGTCGCCTAAAAACTCAGTTTGCCAGTGCCACCTTCTGCTTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:741635/140‑1 (MQ=255)

                                                                 cGACTCATCAGTCGCATTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGTGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:599978/140‑1 (MQ=255)

                                                                   aCTCATCAGTCGCCTTATAAATCAGTTTGCCCGCGCCGCTTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:572675/140‑1 (MQ=255)

                                                                   aCTCATCAGTCGCCTTATAAATCAGTTTGCCAGCGCCGCATTCTGCGTAGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:988706/140‑1 (MQ=255)

                                                                   aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:2105446/140‑1 (MQ=255)

                                                                   aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  1:358413/1‑140 (MQ=255)

                                                                              gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  2:37201/1‑140 (MQ=255)

                                                                              gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    <  2:1428347/140‑1 (MQ=255)

                                                                               ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAGGGCTCGCGTTCGGCGGTCCCCGGCACTTCAATGATgc                                                   >  1:1062280/1‑140 (MQ=255)

                                                                               ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                   >  1:1779702/1‑140 (MQ=255)

                                                                                cTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                  <  1:1549673/140‑1 (MQ=255)

                                                                                 ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 >  1:1449462/1‑140 (MQ=255)

                                                                                   aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc                                               >  2:1581308/1‑140 (MQ=255)

                                                                                        tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                          <  1:214585/140‑1 (MQ=255)

                                                                                         cAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                         <  1:1159747/140‑1 (MQ=255)

                                                                                            tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:1785517/140‑1 (MQ=255)

                                                                                            tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:1449462/140‑1 (MQ=255)

                                                                                            tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:2217773/140‑1 (MQ=255)

                                                                                            tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:37201/140‑1 (MQ=255)

                                                                                               gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  2:1467483/140‑1 (MQ=255)

                                                                                               gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  2:1224587/140‑1 (MQ=255)

                                                                                                 cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 <  1:1598190/140‑1 (MQ=255)

                                                                                                   gcgcCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  2:1899434/140‑1 (MQ=255)

                                                                                                         ccTTCTGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                         <  2:1190628/140‑1 (MQ=255)

                                                                                                              tGCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGCGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                    >  1:1137184/1‑140 (MQ=255)

                                                                                                               gCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 <  1:361367/126‑1 (MQ=255)

                                                                                                               gCGTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 >  2:361367/1‑126 (MQ=255)

                                                                                                                 gTCGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                 >  1:1148977/1‑140 (MQ=255)

                                                                                                                   cGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGTTGCCCCCGTCTTCGGTAATCACTACGTTCATGTCGGtct               >  2:938204/1‑140 (MQ=255)

                                                                                                                   cGCTACAATGGATTCG‑ATTCC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCCGCCGTCCCCTGCCCTTCAATGATGCGCCCTTCTTCGGTGATCAGTACGTTCATGTCGGtct               >  2:807927/1‑140 (MQ=255)

                                                                                                                        cAATGGATTCG‑ATT‑CGCCC‑TCGTGCTAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCg          <  1:938204/140‑1 (MQ=255)

                                                                                                                             gattcg‑attcC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga     <  2:358413/140‑1 (MQ=255)

                                                                                                                                tcg‑attcC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  2:5172/1‑140 (MQ=255)

                                                                                                                                tcg‑attcC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  2:441976/1‑140 (MQ=255)

                                                                                                                                tcg‑attcC‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  2:1595460/1‑140 (MQ=255)

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑C‑CCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815746‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A