Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A38 F60 I1 R1	14	95.7	3260838	99.2%	3234751	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	98.7%	283.4 / ‑3.5	74	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (31/42); total (32/42)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815748‑3816012

cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑ccccctcg                                                                                                                               >  1:989562/1‑136 (MQ=255)

  ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCGTTCTGCGACGCGACAATGGATACG‑ATT‑ccccctcctg                                                                                                                             >  1:668303/1‑134 (MQ=255)

  ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTCCTGCGTCGCTACGATGGGTTCG‑ATTCCCCCTCggg                                                                                                                              >  2:516262/1‑140 (MQ=255)

   gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGc                                                                                                                             <  2:1053132/140‑1 (MQ=255)

   gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGc                                                                                                                             <  1:1142456/140‑1 (MQ=255)

    cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGcc                                                                                                                            >  2:1596892/1‑140 (MQ=255)

      cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGTATTCG‑ATTCCCCCTCGGGCCag                                                                                                                          >  1:585013/1‑140 (MQ=255)

      cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCag                                                                                                                          <  1:1509999/140‑1 (MQ=255)

      cTGATATGGTTTCATGCCTTCGCTCCTCATCTCACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCag                                                                                                                          <  2:1392/140‑1 (MQ=255)

        gATATGGTTTCATGCTTTCGCTACTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCagag                                                                                                                        <  1:532246/140‑1 (MQ=255)

        gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCagag                                                                                                                        <  1:1551238/140‑1 (MQ=255)

          tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGcc                                                                                                                      <  1:1592159/140‑1 (MQ=255)

          tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCG‑CTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCca                                                                                                                     <  2:56645/140‑1 (MQ=255)

          tatGGTTTCATGCCTGCGCTCCTCACCTTTCTTTTCTTCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGcc                                                                                                                      <  1:583465/140‑1 (MQ=255)

             ggTTTCATGCCTTCGTTCCTCATCTGACTTTTCTACATACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCcaac                                                                                                                   <  1:778666/140‑1 (MQ=255)

              gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCcaaca                                                                                                                  <  1:891959/140‑1 (MQ=255)

                 tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGa                                                                                                               >  1:808259/1‑140 (MQ=255)

                  cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGAt                                                                                                              >  2:1203329/1‑140 (MQ=255)

                   aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:1313313/1‑140 (MQ=255)

                   aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                             >  2:1608287/1‑140 (MQ=255)

                   aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                             >  2:1516589/1‑140 (MQ=255)

                   aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:1292363/1‑140 (MQ=255)

                    tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGa                                                                                                            >  2:362968/1‑140 (MQ=255)

                    tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGa                                                                                                            <  1:1608287/140‑1 (MQ=255)

                    tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGa                                                                                                            <  2:1445639/140‑1 (MQ=255)

                        ttCGCTCCTCATCTTGCTTTTCTACTGACTAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:676308/140‑1 (MQ=255)

                        ttCGCTCCTCATCTTACTTTTCTACAGACAATAAATAGGTGACTCATCCGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:644785/140‑1 (MQ=255)

                        ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:40749/140‑1 (MQ=255)

                        ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:261120/140‑1 (MQ=255)

                          cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCT‑ATTCCCCCTCGGGCCAGAGCCTACAAGATGAGTAGct                                                                                                      >  1:657416/1‑140 (MQ=255)

                          cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                                                                                      <  1:305381/140‑1 (MQ=255)

                               ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                                                                                 >  1:1316885/1‑140 (MQ=255)

                                 cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                               >  2:1406623/1‑140 (MQ=255)

                                    cTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                                                                            >  2:43633/1‑140 (MQ=255)

                                     ttACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                                                                           <  1:43633/140‑1 (MQ=255)

                                      tACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGa                                                                                          <  1:915763/140‑1 (MQ=255)

                                       aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGAATC‑CCCCTCGGGCGAGAGCCAACAAGATGAGGAGCTCTTCATGGGTGaa                                                                                         >  2:494900/1‑140 (MQ=255)

                                       aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCAAACTAGCTGAGTAGCTCTTCATGGGTGaa                                                                                         >  1:753037/1‑140 (MQ=255)

                                         ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                                                                                       >  2:283372/1‑140 (MQ=255)

                                           ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                     >  1:352713/1‑140 (MQ=255)

                                           ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                     <  2:585013/140‑1 (MQ=255)

                                            tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    >  1:1081106/1‑140 (MQ=255)

                                            tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    <  1:362968/140‑1 (MQ=255)

                                             cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                                                                   >  1:1543136/1‑140 (MQ=255)

                                              tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  >  2:1485778/1‑140 (MQ=255)

                                                       aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                         >  2:956652/1‑140 (MQ=255)

                                                             ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  2:844456/1‑140 (MQ=255)

                                                             ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  1:1135953/1‑140 (MQ=255)

                                                             ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  2:1386497/1‑140 (MQ=255)

                                                               cGACTCATCAGTCGCTTTAAAAATCAGTTCGCCAGCGACGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:737218/140‑1 (MQ=255)

                                                               cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1316885/140‑1 (MQ=255)

                                                               cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:1496784/140‑1 (MQ=255)

                                                               cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:229554/140‑1 (MQ=255)

                                                               cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCACTTCATGCGTGGACGGCTCGCGTTCTGCCGTCCCCTg                                                                 >  1:495925/1‑140 (MQ=255)

                                                                 aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:1313313/140‑1 (MQ=255)

                                                                 aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:1404083/140‑1 (MQ=255)

                                                                           cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgat                                                     <  1:283372/140‑1 (MQ=255)

                                                                           cGCCTTAAAAATCAGTTTGCCAGCGCCGCCTCCTGTGTCTCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgat                                                     <  2:560437/140‑1 (MQ=255)

                                                                                    aaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTc                                            >  2:974467/1‑140 (MQ=255)

                                                                                     aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                           <  2:1543136/140‑1 (MQ=255)

                                                                                      tCAGTTTGCCAGCGCGGCCTTCTGCCTCGCTACAATGTATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                          <  1:490858/140‑1 (MQ=255)

                                                                                      tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                          <  1:801760/140‑1 (MQ=255)

                                                                                       cAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                         <  2:1081106/140‑1 (MQ=255)

                                                                                        aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:1315255/1‑140 (MQ=255)

                                                                                        aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:1147848/1‑140 (MQ=255)

                                                                                         gTTTGCCAGCGCCGCCATCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       <  2:988484/140‑1 (MQ=255)

                                                                                          tttGCCAGCGCCGCCTTTTGCGTCGCTACAACGGATTCG‑ATTCCCCCTAGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:495925/140‑1 (MQ=255)

                                                                                          tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      >  2:2802/1‑140 (MQ=255)

                                                                                          tttGCCAGCGCCGCCCTCTGCGACGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:494900/140‑1 (MQ=255)

                                                                                            tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                    >  2:1220471/1‑140 (MQ=255)

                                                                                               cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 <  1:1516589/140‑1 (MQ=255)

                                                                                                aGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                <  1:1485778/140‑1 (MQ=255)

                                                                                                    ccgccTTCTGCGTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCGGCCGTCCCATGCGCTTGAATGATGCCCCCGCTTTCGGTCATCACTACg                            >  2:744683/1‑140 (MQ=255)

                                                                                                               gTCGCTACAATGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                 <  2:808259/140‑1 (MQ=255)

                                                                                                                         tGGATTCG‑ATTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa       <  2:1011569/140‑1 (MQ=255)

                                                                                                                          ggATTCG‑ATTCCCCCTCGGGCCCGATCCATCAAGATGTGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag      <  1:516262/140‑1 (MQ=255)

                                                                                                                           gattcg‑attcCCCCTCGGGCCAAAGCCAACAAGATGAGTTGCTCTTCACGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga     <  2:657416/140‑1 (MQ=255)

                                                                                                                             ttcg‑attcCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt   >  1:296995/1‑140 (MQ=255)

                                                                                                                              tcg‑attcCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  1:207935/1‑140 (MQ=255)

CTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑ATT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815748‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A