Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F60 I1 R1	12	49.9	1742736	98.3%	1713109	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	100.0%	97.6 / NA	27	E224G (GAA→GGA)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (14/13); total (14/13)

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCT  >  NC_000913/3815741‑3816013

cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                                                                                                                         <  2:11630/140‑1 (MQ=255)

cAATAAACTGGCGATGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                                                                                                                         <  1:170138/140‑1 (MQ=255)

     aaCTGGCGCTGATATGGTTTCAGGCCTTCGCTCCTTATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑ccct                                                                                                                                  <  2:322223/140‑3 (MQ=255)

         ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTCCCAGCGCCGCCTCCTGGGTCGCTACCATGGATTCGATTCC‑C‑CCTCggg                                                                                                                               >  2:277292/1‑140 (MQ=255)

               gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCagag                                                                                                                         <  2:591761/140‑1 (MQ=255)

                 tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGcc                                                                                                                       <  2:205749/140‑1 (MQ=255)

                    ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCcaac                                                                                                                    >  2:745348/1‑140 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGGTT‑CAC‑CCTCGGGCGAGAGCCAACAAGATg                                                                                                              >  1:375670/1‑140 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATg                                                                                                              >  1:99225/1‑140 (MQ=255)

                               ttCGCTCCTCATCTTAGTTTTCAACAGACAAAAAATAGGAGACTCATCTGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  2:375670/140‑1 (MQ=255)

                                  gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                                                                                      >  2:831222/1‑140 (MQ=255)

                                              aCTTTTCTACAGACAAAGAAAAGGCGACTCAGCAGCCGCTTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                                                                          <  2:350991/140‑1 (MQ=255)

                                                  ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                      >  2:2033/1‑140 (MQ=255)

                                                   tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                     >  1:157853/1‑140 (MQ=255)

                                                     tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGTCAACAAGTTGAGTATCTCTTCTTGGGTGAACGGCTCg                                                                                   >  1:421311/1‑140 (MQ=255)

                                                     tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCACGGGCCAGGGCCAACAAGATGAGTAGCTCTGCATGGGTGAACGGCTCg                                                                                   >  2:302649/1‑140 (MQ=255)

                                                       cAGACAAAAAAAAGGTGACTCAGCAGTCGGCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                 <  1:403358/140‑1 (MQ=255)

                                                                   aGGCGACTCATCAGTTGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                     <  1:280105/140‑1 (MQ=255)

                                                                   aGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                     <  2:307805/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAACTCCGTTCGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATTAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:277292/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:99225/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:745348/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:793018/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCACTTCATGGGTGAACGGCTCGCATTCTGCCGTCCCCTg                                                                  >  1:338782/1‑140 (MQ=255)

                                                                                    ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                    >  1:96314/1‑140 (MQ=255)

                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                  >  2:496328/1‑140 (MQ=255)

                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCGTCTTCCGACCCCTGCAATTCAGTGATGCGCCCGTCTTCg                                         >  2:243493/1‑140 (MQ=255)

                                                                                                  tcgcgaGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CACCACGGGCCAGCGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCGGTCCCCTCGACTTCAATGATGCGCCCGTCCTCGGtc                                      <  2:499622/135‑1 (MQ=255)

                                                                                                      cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                  >  2:611078/1‑140 (MQ=255)

                                                                                                                      gTCGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                  <  2:157853/140‑1 (MQ=255)

                                                                                                                        cGCTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct                <  2:96314/140‑1 (MQ=255)

                                                                                                                          cTACAATGGATTCGATTCC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct              >  2:678663/1‑140 (MQ=255)

                                                                                                                                  gattcgattcC‑C‑CCTCGGGCCACAGTCAACAAGATGACTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga      <  1:243493/140‑1 (MQ=255)

                                                                                                                                     tcgattcC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                  <  1:634652/125‑1 (MQ=255)

                                                                                                                                     tcgattcC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                  >  2:634652/1‑125 (MQ=255)

                                                                                                                                      cgattcC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt  <  1:496328/140‑1 (MQ=255)

                                                                                                                                      cgattcC‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt  <  1:831222/140‑1 (MQ=255)

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑CCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCT  >  NC_000913/3815741‑3816013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A