Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F60 I1 R1	13	95.5	3214742	98.9%	3179379	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	95.3%	155.4 / ‑0.2	43	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (18/23); total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATT‑CCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815741‑3816012

cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGATCCCCATCTTACCTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattc‑gatt‑c                                                                                                                                       <  2:665909/140‑1 (MQ=255)

         ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCggg                                                                                                                              >  1:1217769/1‑140 (MQ=255)

         ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCATTCTGCGCCGCGACCATGGATAC‑GATTCCCCC‑TCggg                                                                                                                              >  1:776217/1‑140 (MQ=255)

          gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGc                                                                                                                             <  2:1104923/140‑1 (MQ=255)

              tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTACTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCaga                                                                                                                         <  1:492253/140‑1 (MQ=255)

              tGATATGGTTTCATGCCCTCGCGCCTCATCTCACTCTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCaga                                                                                                                         <  2:557973/140‑1 (MQ=255)

                    ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCcaac                                                                                                                   <  1:96215/140‑1 (MQ=255)

                       ttCATGCCTTCGCTCTTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAg                                                                                                                <  1:577826/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:229368/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCCACAGACAAAAAACAGGCGACTCATCTGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:649773/140‑1 (MQ=255)

                                 cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑AATT‑CCCCATCGAGCGAGAGCCGACAAGATGAGTAGct                                                                                                      >  1:475970/1‑140 (MQ=255)

                                    tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                   <  2:1225529/140‑1 (MQ=255)

                                           cTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                                                                            >  1:293871/1‑140 (MQ=255)

                                              aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAg                                                                                                           >  2:991701/1‑122 (MQ=255)

                                              aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAg                                                                                                           <  1:991701/122‑1 (MQ=255)

                                                  ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                     >  1:1369792/1‑140 (MQ=255)

                                                  ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                     <  1:1256106/140‑1 (MQ=255)

                                                    cTACAGACAAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                                                                   <  2:293871/140‑1 (MQ=255)

                                                     tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  >  1:178121/1‑140 (MQ=255)

                                                     tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTAGCCAGCGCCGCTTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  >  2:977004/1‑140 (MQ=255)

                                                      aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                 <  1:53460/140‑1 (MQ=255)

                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCTCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                             >  2:171664/1‑140 (MQ=255)

                                                                   aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    <  2:178121/140‑1 (MQ=255)

                                                                    ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTACCTCTTCAAGGGGGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  1:720777/1‑140 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGTCCGCGCCCCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:720777/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCTTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:977004/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                              >  1:878243/1‑127 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                              <  2:878243/127‑1 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCGCTGCa                                                               >  2:1489685/1‑140 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  2:803664/1‑140 (MQ=255)

                                                                                   gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    <  1:803664/140‑1 (MQ=255)

                                                                                    ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                   >  1:856239/1‑140 (MQ=255)

                                                                                     cTTAAAAATCAGTTTGCTAGCGCCGCCTTCTCCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                  <  2:425321/140‑1 (MQ=255)

                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAGGGCTCGCGTTCTGCCGGCCCCAGCACTTCAATGATgcgc                                                 >  2:515139/1‑140 (MQ=255)

                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTACTGCCGACCCCTGTACTTCAATGATgcgc                                                 >  1:521809/1‑140 (MQ=255)

                                                                                                 tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:856239/140‑1 (MQ=255)

                                                                                                   tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                    >  1:1076248/1‑140 (MQ=255)

                                                                                                        gcgcCGCCTTCTGCGTGTCTACCATGGATTCTAATG‑CCCT‑TCGGGCCAAAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  2:776217/140‑1 (MQ=255)

                                                                                                        gcgcCGCCTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  2:1217769/140‑1 (MQ=255)

                                                                                                          gccgccTTCTGCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTAc                             <  2:1369792/140‑1 (MQ=255)

                                                                                                                    gCGTCGCTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   <  1:1489685/140‑1 (MQ=255)

                                                                                                                          cTACAATGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct             >  1:1256102/1‑140 (MQ=255)

                                                                                                                               aTGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCAGTCATAGCTCATCACTCCGTTTGTGTCGGTCTCTGCgac        >  2:716632/1‑138 (MQ=255)

                                                                                                                                tGGATTC‑GATTCCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa       <  2:1256102/140‑1 (MQ=255)

                                                                                                                                    ttc‑gattcCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTACCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt   >  1:1304061/1‑140 (MQ=255)

                                                                                                                                     tc‑gattcCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCagaggc  >  2:1217518/1‑138 (MQ=255)

                                                                                                                                     tc‑gattcCCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACGACGATCCTGTCGGACTCTGCGGCAGAGTc  >  1:506604/1‑140 (MQ=255)

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTC‑GATT‑CCCC‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815741‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A