Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F0 I0 R1 81 326.4 10500568 93.1% 9776028 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,463,915 C→A 28.4% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,463,9150CA28.4% 145.7 / 30.1 70Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base A (18/3);  total (44/26)
Fisher's exact test for biased strand distribution p-value = 1.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATT  >  CP009273/2463775‑2464061
                                                                                                                                            |                                                                                                                                                   
gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                            <  1:2129993/150‑1 (MQ=255)
 aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAAAAAAGaaaaaaa                                                                                                                                           >  2:5039475/1‑147 (MQ=255)
   tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATTAAATAAATAAAATAAAAGAAAAAAATAgag                                                                                                                                         >  2:4289059/1‑148 (MQ=255)
    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                        <  2:3662786/150‑1 (MQ=255)
    atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                        <  2:1038467/150‑1 (MQ=255)
     tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                       <  1:1709138/150‑1 (MQ=255)
     tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTTTAAAAAAAATCAAATAAATAAAAACaaaaaccaa                                                                                                                                       >  2:4596379/1‑143 (MQ=255)
     tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTCTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                       <  1:1709184/150‑1 (MQ=255)
      ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTTTTTTTAAAAAAATTAAAATAAAAAAAAACAATatagatt                                                                                                                                      >  2:2720201/1‑144 (MQ=255)
        cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                                                                                                    <  1:521191/150‑1 (MQ=255)
         aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAAAAAAGACAATAAAGATGTaa                                                                                                                                   >  2:3031913/1‑150 (MQ=255)
         aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAATAAAAAAAGACAAAAACGCTGtta                                                                                                                                   >  2:3031929/1‑148 (MQ=255)
             ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTTAAATTATTAAAATAAAAAAATACAAAAAAGATTTAAATCa                                                                                                                               >  1:1605641/1‑150 (MQ=255)
                tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAAAAACAAAAAAAAGATGTaaaaaacaa                                                                                                                            >  2:3305656/1‑144 (MQ=255)
                     cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                       <  2:1490661/150‑1 (MQ=255)
                     cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                       <  2:1490373/150‑1 (MQ=255)
                          ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATTAAAAAATTAAAATAAAAAAATAAAAAATAGATTTAAATAACAAAGTTT‑TAAtt                                                                                                                 >  1:861825/1‑150 (MQ=255)
                          ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTTTTTATAAAATAAATAAAATAAAAAAAAACAAAAACCATTTAAATAAAAAAATTT‑AAAta                                                                                                                 >  1:5007927/1‑149 (MQ=255)
                          ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAATAAATAAAAAAAAAAAAAAAAAAAAAGATTTAAATCAAAAATTTT‑AAAtt                                                                                                                 >  1:3904546/1‑150 (MQ=255)
                                gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAATAATTAAAACAAATAAACACAAAAACCAGTTAAAGCACTAAGATT‑AAAtatagcgc                                                                                                           >  2:5171261/1‑143 (MQ=255)
                                gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAATAAATCAATAAAAAAACAATTTAAAAAAAAAAGTTT‑AAATTtcgcgc                                                                                                           >  2:5171328/1‑150 (MQ=255)
                                        taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAATACAAAACAGATGTAAAGCACAAAGTTT‑TAAATTCGCGCCaaaaaat                                                                                                   >  2:2505726/1‑145 (MQ=255)
                                                    aaagaaTTTTTTTGGATTGTATTTTTCGTTCGACATTTGCTTATAAATAACATTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATAAATAAAATAGCTATTTAAAGCACTAATTTT‑AATTTGCGCGCCAATCATGGCGCGCACAAGc                                                                                       <  1:5139681/149‑1 (MQ=255)
                                                     aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTTTTTTTAAAAAAAATAAAAAAAAAAAAAAAAAAAAAGAAGTAAAGAACAAAATTT‑TAATATTGCGCCACAAATGGCGCGcccaccca                                                                                      >  1:3307868/1‑146 (MQ=255)
                                                           ttatttGTAATTTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                                <  2:1501495/150‑1 (MQ=255)
                                                           ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                                <  2:1501489/150‑1 (MQ=255)
                                                               ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTAAAAAAAAAATAAAACAAAAAAATAAAAAATCCAAGTAAACACCAAAGTTTAAAATTGC‑CGCCAATAAAGGCGCGCCCAAACAATAAAAACaa                                                                            >  2:1527493/1‑150 (MQ=255)
                                                               ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAAAAATAAAGAAAAAATAGCTGTAAAGCACTAAGTTT‑AAAATTAGCGCCAAACATGGCGCGCCCAAACAATAAAACaaa                                                                            >  2:4164440/1‑150 (MQ=255)
                                                                      ttATTTTTCGTTCGACTTTTGATTATAAATAAAAGTCTTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAATAGCTATGTAAAGCAATATGTTT‑AATTTGCGCGCAAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                     <  1:240409/149‑1 (MQ=255)
                                                                      gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                     <  2:4457390/150‑1 (MQ=255)
                                                                      gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAAAAATACAAAATAGATGTAAATAACAAATTTT‑AAATTTAGCGCCAAAAAATGCGCGCACAAACAATAAAAACaaacaaaaa                                                                     >  2:2002934/1‑143 (MQ=255)
                                                                      gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAATTAAAATAAAAAAAAAAAAAATAGATTTAAAACAAAAAGTTT‑AAATATAGCGCCAAAAAATGCGCGCACAAGATATAAAAACaaacaaaat                                                                     >  2:2443304/1‑143 (MQ=255)
                                                                          ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAAAAATAAATAAAATATCGATTTTTATAAATAAGTTT‑AAATTTGGGGCCAAAAATGTGGGGCGCAAGCTATAATAAAAAACAAAtttata                                                                 >  2:5021474/1‑147 (MQ=255)
                                                                           tttGGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                <  2:4920102/150‑1 (MQ=255)
                                                                              cgttcgACCTTTGCTTATACATAACATTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGAACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                             <  2:1416004/150‑1 (MQ=255)
                                                                              cgttcgACCTTTGCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                             <  2:1416003/150‑1 (MQ=255)
                                                                                             tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                              <  1:1596115/150‑1 (MQ=255)
                                                                                                   taaccttCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                        <  1:2841671/144‑1 (MQ=255)
                                                                                                          cGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                                 >  1:5149386/1‑150 (MQ=255)
                                                                                                             ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                              >  1:2013191/1‑150 (MQ=255)
                                                                                                             ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                              <  1:435445/150‑1 (MQ=255)
                                                                                                             ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                              <  1:3428788/150‑1 (MQ=255)
                                                                                                             ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                              <  1:433766/150‑1 (MQ=255)
                                                                                                                      ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                     >  1:745911/1‑150 (MQ=255)
                                                                                                                        ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                   >  1:4220050/1‑150 (MQ=255)
                                                                                                                         taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                  >  2:410333/1‑150 (MQ=255)
                                                                                                                           aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATCATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                >  1:1840195/1‑150 (MQ=255)
                                                                                                                             ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa              <  2:861825/150‑1 (MQ=255)
                                                                                                                             ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCCCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa              >  2:2906453/1‑150 (MQ=255)
                                                                                                                              taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGTCTATATCTCCTGATAGAATTGCGGTATTGACTATCaa             >  1:4179155/1‑150 (MQ=255)
                                                                                                                              taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa             >  1:1351381/1‑150 (MQ=255)
                                                                                                                              taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa             >  1:1351432/1‑150 (MQ=255)
                                                                                                                              taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa             >  1:1351442/1‑150 (MQ=255)
                                                                                                                              taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGGATTGACTATcca             >  2:157558/1‑148 (MQ=255)
                                                                                                                                atacAAAGAAATAAACACAATAGCGATGAAAAGCACTAAGTTT‑AAATTGCGCGCCAATCAGGGCCCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt           <  1:4816435/147‑1 (MQ=255)
                                                                                                                                  tCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc         <  1:5171328/150‑1 (MQ=255)
                                                                                                                                  tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAATTGc         >  2:3348936/1‑150 (MQ=255)
                                                                                                                                  tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc         <  1:5171261/150‑1 (MQ=255)
                                                                                                                                  tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc         >  2:3347235/1‑150 (MQ=255)
                                                                                                                                   cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc        >  1:2360523/1‑150 (MQ=255)
                                                                                                                                   cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc        >  1:2228338/1‑150 (MQ=255)
                                                                                                                                   cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc        >  2:2116494/1‑150 (MQ=255)
                                                                                                                                    aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc       >  1:3905733/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTATTGACTATCAAATGCCCt      >  2:2082185/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt      >  1:1501236/1‑150 (MQ=255)
                                                                                                                                     aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt      >  2:2081088/1‑150 (MQ=255)
                                                                                                                                       gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa    <  2:4330960/150‑1 (MQ=255)
                                                                                                                                        aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTCTCAAATGCCCTGAt   >  2:2644821/1‑150 (MQ=255)
                                                                                                                                        aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt   >  1:2507893/1‑150 (MQ=255)
                                                                                                                                         aaTCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAtt  >  2:3612670/1‑150 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
GAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTT‑AAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATT  >  CP009273/2463775‑2464061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A