Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,463,915 C→A 11.5% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,463,9150CA11.5% 663.6 / 17.8 212Q436K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (63/114);  new base A (26/6);  total (90/122)
Fisher's exact test for biased strand distribution p-value = 2.86e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCG  >  CP009273/2463767‑2464063
                                                                                                                                                     |                                                                                                                                                    
     ttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAATAAAACAATAc                                                                                                                                                 >  2:2152246/1‑149 (MQ=255)
         aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                             <  2:2264570/149‑1 (MQ=255)
          atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                                                                                                            <  2:4816678/149‑1 (MQ=255)
           tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                                                                                                           <  1:2790614/149‑1 (MQ=255)
           tatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                                                                                                           <  1:2789655/149‑1 (MQ=255)
            atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                                                                                                          <  1:1677195/149‑1 (MQ=255)
            atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAAATAAAAGAAAAAAATACaaaatct                                                                                                                                          >  2:3808873/1‑144 (MQ=255)
                   ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                   <  1:3488472/149‑1 (MQ=255)
                      tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTTTAAAAAAATTAAAAAAAAAAAAAAAAAAAACGATGTaaaaaa                                                                                                                                >  1:1263196/1‑146 (MQ=255)
                      tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                                <  1:2681508/149‑1 (MQ=255)
                      tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAATAAATAAATACAAAATAGATTTAAATCa                                                                                                                                >  2:2593640/1‑149 (MQ=255)
                        tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                                                                                                              <  2:962484/149‑1 (MQ=255)
                             cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                         <  1:2319731/149‑1 (MQ=255)
                             cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                         <  1:4113340/149‑1 (MQ=255)
                             cATTTTTCGGGGAAGGATATAGAGAATTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                         <  1:4113296/149‑1 (MQ=255)
                               tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                                                                                                       <  1:2512312/149‑1 (MQ=255)
                               tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                                                                                                       <  1:98413/149‑1 (MQ=255)
                                 tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                     <  2:2376448/149‑1 (MQ=255)
                                       ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                               <  2:3323111/149‑1 (MQ=255)
                                            gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTTATTTATAAAATAAATCAAAAAAATCAAGACAATATCGATTTTAATCAATAAGTTTTAATTTAGCGCCaa                                                                                                          >  2:4573640/1‑149 (MQ=255)
                                             atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAGAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                                                                                                         <  2:2115203/149‑1 (MQ=255)
                                             atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAAATAAAATAAATAAAGACAAAAGAGATGTTAAGCACTAATTTTTAATTTAGCGCCact                                                                                                         >  1:795702/1‑147 (MQ=255)
                                               ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                       <  2:1640033/149‑1 (MQ=255)
                                               ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                       <  2:3214494/149‑1 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACTTAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                    <  2:716945/149‑1 (MQ=255)
                                                  gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                    <  2:3708798/149‑1 (MQ=255)
                                                            caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTGTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAGGTAAAGCACTAAGTTTAAATGGCGCGCCAATCATGGCGCGCACAAg                                                                                          <  2:523518/149‑1 (MQ=255)
                                                             aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAAACAATAAAATATAGAAGTAAATCACTAATTTTAAAATTAGCGCCAATCATGGCGCGCACACGc                                                                                         >  2:4712548/1‑149 (MQ=255)
                                                               gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACCGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                       <  2:1221548/149‑1 (MQ=255)
                                                                  tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAAATAAAAAAAAAAAAAAAAAAATAGATGTAAATAAAAAATTTTAAATTTGGCGGCAAGCAAGTGGCGCCCAACCTataa                                                                                    >  1:2163827/1‑149 (MQ=255)
                                                                        tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                              <  2:4325621/149‑1 (MQ=255)
                                                                        tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAAAAATTAAAATAAAAAAATAAAAAAACGATGTAAAGCAAAAATTTTAAATTTCGCGCCAAAAATGGCGCGCACAAACAATAAAAACaa                                                                              >  2:1060183/1‑149 (MQ=255)
                                                                        tGGAATGTATTTTTCGTTCGACCTTTGATTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                              <  1:4262936/149‑1 (MQ=255)
                                                                         ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                             <  2:504404/149‑1 (MQ=255)
                                                                           aaTGTATTTTTCGTTCGACCTTTGATTATAAATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAATATGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCt                                                                           <  1:542648/149‑1 (MQ=255)
                                                                             tttATTTTTCGTTCGACCTTTGCTTATACATAACATTCG‑TTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGTTGTAAAGAACTATTTTTAAATTGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCAACCTaa                                                                         <  1:1908945/147‑1 (MQ=255)
                                                                             tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAATAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTaa                                                                         <  1:5135676/149‑1 (MQ=255)
                                                                             tGTATTTTTCGTTCGACCTTTGCTTATAAATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTaa                                                                         <  2:3507056/149‑1 (MQ=255)
                                                                              gTATTTTTCTTTCGACCTTTTATTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAATAGCGTTTTAAAGAAATAATTTTAAATTGGGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                        <  1:489036/149‑1 (MQ=255)
                                                                              gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTTTTTTATAAAAAAATTAAAACAAAAAAATACAAAATCGATGTAAAACACAAAGTTTTAATTTAGCCCCAAACAATGCGCCCACACAAGATAATAACAACCaaaa                                                                        >  2:1506649/1‑148 (MQ=255)
                                                                              gTATTTTTCGTTCGACCTTTGCTTATAAATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                        <  2:4018901/149‑1 (MQ=255)
                                                                               tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                        <  2:2306330/149‑1 (MQ=255)
                                                                                       gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                               <  2:2258876/149‑1 (MQ=255)
                                                                                       gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                               <  2:4864721/149‑1 (MQ=255)
                                                                                       gttcgACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                               <  2:2705798/149‑1 (MQ=255)
                                                                                        ttcgACCTTTGCTTATAAATAACATTCT‑TTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                              <  1:4103203/149‑1 (MQ=255)
                                                                                         tcgACCTTTGATTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                             <  1:4182810/149‑1 (MQ=255)
                                                                                           gACCTTTGCTTATACAAAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                           <  1:2095526/149‑1 (MQ=255)
                                                                                             ccTTTGCTTATACATAACAGTCG‑TGTTTTTAATTTATAAAATAAATAAAACAAAAAAAGACAAAAGCGGTGTAAATCACAAACTTTAAATTGGGCGCCACACATGGCGCGCCCAAGCTAAAATAACAACCAAAATTATCCACCACTTag                                                         >  2:3979364/1‑149 (MQ=255)
                                                                                                ttGCTTATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                      <  1:729219/149‑1 (MQ=255)
                                                                                                   attATACATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                   <  1:2745255/148‑1 (MQ=255)
                                                                                                    ttATAAATAACATTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTGAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                                  <  1:712766/149‑1 (MQ=255)
                                                                                                     tataAATAACAGTCT‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                 <  1:2135765/149‑1 (MQ=255)
                                                                                                     tataAATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                 <  1:4730411/149‑1 (MQ=255)
                                                                                                      ataCATAACAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGCTATTTAAAGAACTAAGTTTAAATTGCGCGCAAATCATGGCGCGCACAAGATATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                                <  1:101195/149‑1 (MQ=255)
                                                                                                       taCATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtata                                               <  2:4545200/149‑1 (MQ=255)
                                                                                                            aaCAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                          <  1:4595056/149‑1 (MQ=255)
                                                                                                            aaCAGTCG‑TTTTTTTAATTTATAAAATAATTAAAAAAAAAAAATACAAAATCGATGTAAATAACTAATTTTTAATTTCGCGCCAATAATTGCGCGCACAACCAAAAAAAACAACCAAAATTCTCCACCTCGTAGAGTGAATaaataaac                                          >  2:2988398/1‑145 (MQ=255)
                                                                                                                  cG‑TTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCTATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                    <  1:972005/149‑1 (MQ=255)
                                                                                                                   g‑TTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                                   >  1:4141033/1‑149 (MQ=255)
                                                                                                                     tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                  <  1:623467/149‑1 (MQ=255)
                                                                                                                     tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                                  >  1:3069827/1‑149 (MQ=255)
                                                                                                                      ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                 <  2:2966302/149‑1 (MQ=255)
                                                                                                                       tttttAATTTATAAAATAATTCAAATAAATCAAGACAATAGCTTTGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAGGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                                <  1:1822826/149‑1 (MQ=255)
                                                                                                                        ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                               <  1:2593626/149‑1 (MQ=255)
                                                                                                                          ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                             <  1:2544121/149‑1 (MQ=255)
                                                                                                                             aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATCGAATTGCGGTa                          >  2:4527655/1‑149 (MQ=255)
                                                                                                                             aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                          >  2:2808387/1‑149 (MQ=255)
                                                                                                                             aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                          >  2:3717272/1‑149 (MQ=255)
                                                                                                                                 ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                      <  2:4215780/149‑1 (MQ=255)
                                                                                                                                 ataAAATAATTCAAAGAAATCAAAACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTATTGa                      >  1:4522245/1‑149 (MQ=255)
                                                                                                                                    aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                   >  2:1088225/1‑149 (MQ=255)
                                                                                                                                    aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                   >  2:3684348/1‑149 (MQ=255)
                                                                                                                                     aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                  >  1:3217979/1‑149 (MQ=255)
                                                                                                                                      ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACtctc                 >  2:3718053/1‑149 (MQ=255)
                                                                                                                                      ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                 >  1:2093103/1‑149 (MQ=255)
                                                                                                                                      ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                 >  1:2093110/1‑149 (MQ=255)
                                                                                                                                       taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                >  2:2854738/1‑149 (MQ=255)
                                                                                                                                        aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa               >  2:3777969/1‑149 (MQ=255)
                                                                                                                                         atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa              >  1:1921592/1‑149 (MQ=255)
                                                                                                                                         atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa              <  2:419249/149‑1 (MQ=255)
                                                                                                                                          ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATGGCGCGCACAAGCTCTAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGACTTGCGGTATTGACTATCAAAt             >  2:1953358/1‑149 (MQ=255)
                                                                                                                                          ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt             >  1:3201923/1‑149 (MQ=255)
                                                                                                                                          ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATGCCACCCTAGTTTCTCCTCCGCTTAGAGTGACTATATCTCCTGATAGAATGGCGGTCTTGACTCTCAAAt             >  2:3264603/1‑149 (MQ=255)
                                                                                                                                          ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt             >  2:4709099/1‑149 (MQ=255)
                                                                                                                                          ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt             <  1:2950125/149‑1 (MQ=255)
                                                                                                                                             aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc          >  2:4166056/1‑149 (MQ=255)
                                                                                                                                             aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc          >  2:1326723/1‑149 (MQ=255)
                                                                                                                                              aataaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc         <  1:3921224/146‑1 (MQ=255)
                                                                                                                                              aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc         <  1:501970/149‑1 (MQ=255)
                                                                                                                                              aaGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc         <  1:985816/149‑1 (MQ=255)
                                                                                                                                                gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg       >  2:563902/1‑149 (MQ=255)
                                                                                                                                                gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg       >  2:564030/1‑149 (MQ=255)
                                                                                                                                                gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg       >  2:1660018/1‑149 (MQ=255)
                                                                                                                                                 aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa      >  1:485884/1‑149 (MQ=255)
                                                                                                                                                  aaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt     >  2:4843798/1‑149 (MQ=255)
                                                                                                                                                   aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAtt    >  1:4382945/1‑149 (MQ=255)
                                                                                                                                                     cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg  <  2:4359543/149‑1 (MQ=255)
                                                                                                                                                     cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg  >  2:284167/1‑149 (MQ=255)
                                                                                                                                                     cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCg  >  1:4140420/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCG  >  CP009273/2463767‑2464063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A