Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F60 I1 R1	19	81.2	2769194	99.2%	2747040	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	94.9%	210.9 / 2.9	59	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (22/34); total (25/34)
Fisher's exact test for biased strand distribution p-value = 7.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815746‑3816012

aaCTGGCGCTGATATCGTTTCATGCGTTCCCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑cc‑cct                                                                                                                                  <  1:599138/140‑3 (MQ=255)

    ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCggg                                                                                                                              <  1:337187/140‑1 (MQ=255)

          gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCagag                                                                                                                        <  2:1236463/140‑1 (MQ=255)

           atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGcc                                                                                                                            >  1:1117205/1‑135 (MQ=255)

           atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGcc                                                                                                                            <  2:1117205/135‑1 (MQ=255)

                  ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAg                                                                                                                >  1:1193822/1‑140 (MQ=255)

                  ttCATGCCTTCGCTCCTCATCTTACATTTCTACAGAC‑GAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAg                                                                                                                <  1:503483/140‑1 (MQ=255)

                    cATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGAt                                                                                                              >  1:167964/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCag                                                                                                                          >  2:1042019/1‑128 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCag                                                                                                                          <  1:1042019/128‑1 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGTGTCGCTACTATGTATTCGATT‑C‑CC‑CTTCGGTACAGAGCCGACAAGATg                                                                                                             >  2:622159/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:9534/1‑140 (MQ=255)

                     aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:128664/1‑140 (MQ=255)

                      tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGa                                                                                                            <  1:1190039/140‑1 (MQ=255)

                         cTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:297145/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:723444/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:237109/140‑1 (MQ=255)

                          ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:331690/140‑1 (MQ=255)

                           tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGc                                                                                                                       <  1:807118/124‑1 (MQ=255)

                           tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGc                                                                                                                       >  2:807118/1‑124 (MQ=255)

                           tCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCCACAATGGATTCGTTT‑CACC‑C‑TCGGGACAGAGCCAACAAGATGAGTAGc                                                                                                       >  2:538626/1‑140 (MQ=255)

                                 ctcATCTTACTTTTCTAGAGAC‑CAAAAAAAGTCGAGTCACCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                                                                                 <  2:551585/140‑1 (MQ=255)

                                   cATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                               >  1:139974/1‑140 (MQ=255)

                                    aTCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                                                                              >  2:171068/1‑140 (MQ=255)

                                       ttACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                                                                           >  2:895226/1‑140 (MQ=255)

                                           ttttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                                                                                       >  1:214065/1‑140 (MQ=255)

                                              tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    >  1:210925/1‑140 (MQ=255)

                                              tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    >  2:1262337/1‑140 (MQ=255)

                                              tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCt                                                                                    >  1:1335812/1‑140 (MQ=255)

                                              tCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CCTC‑TCGGGCCGGAGACAAAAAGATGAGAAGCTCTTCATGGGTGAACGGCt                                                                                    >  2:542574/1‑140 (MQ=255)

                                                     aC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                             >  2:32997/1‑140 (MQ=255)

                                                        aaaaaaaaGGCGACTAATCAGTCGCCTTAAAAATCAGATTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                           <  1:622159/140‑1 (MQ=255)

                                                               aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    <  2:139974/140‑1 (MQ=255)

                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCt                                                                               <  1:1027841/128‑1 (MQ=255)

                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCt                                                                               >  2:1027841/1‑128 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAACTCCGTTTGCCAGCGGCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:506736/140‑1 (MQ=255)

                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1361439/140‑1 (MQ=255)

                                                                  cGACTCAGCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1209058/140‑1 (MQ=255)

                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCAAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  1:1300044/1‑140 (MQ=255)

                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCG‑CTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                   >  2:123993/1‑140 (MQ=255)

                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 >  1:119012/1‑140 (MQ=255)

                                                                                        aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                           <  2:839432/140‑1 (MQ=255)

                                                                                         tCAGTTTGCCAGCGCCGCCTTCTGCTTCGCTACAATGTATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt                                          <  1:542574/140‑1 (MQ=255)

                                                                                            gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATGCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       <  1:522354/140‑1 (MQ=255)

                                                                                               tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                    <  1:895226/140‑1 (MQ=255)

                                                                                               tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                    <  1:1262337/140‑1 (MQ=255)

                                                                                               tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                    <  2:1335812/140‑1 (MQ=255)

                                                                                                   aGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                <  2:119012/140‑1 (MQ=255)

                                                                                                   aGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                <  2:821367/140‑1 (MQ=255)

                                                                                                    gcgcCGCCTTCTGCGTCGCTACGATTGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  1:538626/140‑1 (MQ=255)

                                                                                                             tCTGCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATg                      <  2:210925/140‑1 (MQ=255)

                                                                                                                gCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   >  2:32235/1‑140 (MQ=255)

                                                                                                                gCGTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   <  2:1319792/140‑1 (MQ=255)

                                                                                                                  gTCGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                 <  2:1248342/140‑1 (MQ=255)

                                                                                                                    cGCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct               >  2:895485/1‑140 (MQ=255)

                                                                                                                     gCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtctt              >  1:1011177/1‑139 (MQ=255)

                                                                                                                     gCTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGACGGtctc              <  2:167964/140‑1 (MQ=255)

                                                                                                                      cTACAATGGATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct             <  2:128664/140‑1 (MQ=255)

                                                                                                                            tGGATTCGATTCC‑CC‑C‑TCGGGCAAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa       <  2:1300044/140‑1 (MQ=255)

                                                                                                                             ggATTCGATTCC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag      <  1:171068/140‑1 (MQ=255)

                                                                                                                              gattcgattcC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga     <  1:32235/140‑1 (MQ=255)

                                                                                                                                 tcgattcC‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  1:1381203/1‑140 (MQ=255)

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CC‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815746‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A