Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F60 I1 R1	16	329.6	11292770	99.2%	11202427	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	94.8%	769.6 / 25.6	212	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (6/5); new base C (87/112); total (95/117)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815741‑3816012

cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgatt‑c                                                                                                                                          <  1:1119450/140‑1 (MQ=255)

  aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c                                                                                                                                      >  1:3904926/1‑140 (MQ=255)

    aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c‑cc                                                                                                                                   <  1:249522/140‑2 (MQ=255)

     aaCTGGCGCTGATATGGCTTCACGCCTTCGCCCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c‑cct                                                                                                                                  <  2:1512370/140‑3 (MQ=255)

      aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑c‑c‑cctc                                                                                                                                 <  1:1021454/140‑4 (MQ=255)

        tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCTTCGATAAAATGGAGTCGATT‑c‑c‑c‑cctcgg                                                                                                                               >  1:1578935/1‑135 (MQ=255)

         ggCGCTGATATGGTTTCATGTCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCggg                                                                                                                               >  1:5399385/1‑140 (MQ=255)

          gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGc                                                                                                                              <  2:5122883/140‑1 (MQ=255)

          gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGc                                                                                                                              <  2:4119911/140‑1 (MQ=255)

           cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGcc                                                                                                                             >  1:2846756/1‑140 (MQ=255)

             cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCag                                                                                                                           >  2:4566389/1‑140 (MQ=255)

               gATATGGTTTCATGCCTTCGCTTCTCATCTTGCTTTTCTTCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                         <  2:1867957/140‑1 (MQ=255)

               gATATGGTTTCATGCCTGCGCTTCTCATCTTTCTTTTCTTCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                         <  2:2048671/140‑1 (MQ=255)

               gATATGGTTTCATGCCTACGCTTCTCATCTTGCTTTTCTACAGACAAACAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                         <  2:2254427/140‑1 (MQ=255)

                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGc                                                                                                                        >  2:1155616/1‑140 (MQ=255)

                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGc                                                                                                                        >  2:5055121/1‑140 (MQ=255)

                 tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGcc                                                                                                                       >  2:4339402/1‑140 (MQ=255)

                  atGGTTTCCTGCCTTCCTTCCTAATCTTACCTTTCAACAGACAAAAAACAGGCGACTCATCACTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCca                                                                                                                      <  2:2659802/140‑1 (MQ=255)

                     gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCcaaca                                                                                                                   <  1:4566389/140‑1 (MQ=255)

                      tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTCCAATGGAATCGCTTAC‑C‑C‑CTCAGGCCAGAGCcaacaa                                                                                                                  >  1:2659802/1‑140 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGGTT‑CAC‑C‑CTCGGGCAAGAGCCAACAAGATg                                                                                                              >  1:2430312/1‑140 (MQ=255)

                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCATTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCagag                                                                                                                         >  2:5431420/1‑129 (MQ=255)

                           tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGa                                                                                                             >  1:4603632/1‑140 (MQ=255)

                            gCCTTCGCACCTCATCGTACTTTTCTACAGACAAACAAAGGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAg                                                                                                            <  2:2702154/140‑1 (MQ=255)

                             ccTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGt                                                                                                           <  2:2846756/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTGCTTTTCTACTGACTAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:2285613/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGTCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:5562328/140‑1 (MQ=255)

                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  2:234515/140‑1 (MQ=255)

                               ttCGCTCATCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         >  1:3542965/1‑140 (MQ=255)

                                tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGc                                                                                                        >  2:5635686/1‑140 (MQ=255)

                                 cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGct                                                                                                       <  1:647258/140‑1 (MQ=255)

                                      ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGTTg                                                                                                              <  2:3626295/128‑1 (MQ=255)

                                      ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATg                                                                                                              >  1:3626295/1‑128 (MQ=255)

                                      ctcATCTTACTTTTCTACAGAC‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         >  2:4626536/1‑132 (MQ=255)

                                       tcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                                                                                 >  1:5113092/1‑140 (MQ=255)

                                         aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CGC‑CTAGGGTCAGAGCCAACAAGATGAGTAGCTCTTCAT‑gg                                                                                              >  2:1954127/1‑140 (MQ=255)

                                              aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGaa                                                                                         >  1:4077970/1‑140 (MQ=255)

                                              aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGaa                                                                                         >  2:3848309/1‑140 (MQ=255)

                                                   tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCt                                                                                    >  1:1233261/1‑140 (MQ=255)

                                                   tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCt                                                                                    >  2:3074820/1‑140 (MQ=255)

                                                    cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTc                                                                                   >  2:3996458/1‑140 (MQ=255)

                                                    cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTc                                                                                   <  2:3967181/140‑1 (MQ=255)

                                                     tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCg                                                                                  >  1:345806/1‑140 (MQ=255)

                                                       cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGcc                                                                                <  1:4350866/140‑1 (MQ=255)

                                                         gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCtt                                                                              >  2:652764/1‑140 (MQ=255)

                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGcc                                                                                <  1:3329806/137‑1 (MQ=255)

                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGcc                                                                                >  2:3329806/1‑137 (MQ=255)

                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTc                                                                             >  2:5211463/1‑140 (MQ=255)

                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTc                                                                             >  2:5363291/1‑140 (MQ=255)

                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTc                                                                             >  1:5488461/1‑140 (MQ=255)

                                                            aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTg                                                                           <  2:5250495/140‑1 (MQ=255)

                                                               aaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGGTGAACGGCTCGCCTTCTGcc                                                                         <  1:3706753/140‑1 (MQ=255)

                                                                 aaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTc                                                                      <  1:4536747/140‑1 (MQ=255)

                                                                   aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    <  1:3074820/140‑1 (MQ=255)

                                                                    ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTATCTCTTCAG‑GGGAGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  1:1459776/1‑140 (MQ=255)

                                                                      cGACTCATCAGTCGCTTTAAAACTCCGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:1820769/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAACTCTGTTTGCCAGCGGCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:2204750/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGTGCCTCCTTCTGCTTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:2172599/140‑1 (MQ=255)

                                                                      cGACTCATCAGTCGCCTCAAAAATCAGTTTGCCAGTGCCCCCTTCTGCCTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1459776/140‑1 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:65988/138‑1 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:1817009/140‑1 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  1:5055121/140‑1 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  1:1155616/140‑1 (MQ=255)

                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  1:3848309/140‑1 (MQ=255)

                                                                           catcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCTT‑GGGTGAACGGGTCCCCTTCTACCGTCCCCTGCACtt                                                            >  1:2114383/1‑140 (MQ=255)

                                                                            atcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc                                                           >  1:4633239/1‑140 (MQ=255)

                                                                               aGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 >  1:2919922/1‑131 (MQ=255)

                                                                               aGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:2919922/131‑1 (MQ=255)

                                                                                gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg                                                       <  2:1052770/140‑1 (MQ=255)

                                                                                   gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  2:1266156/1‑140 (MQ=255)

                                                                                    ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc                                                   >  1:4178686/1‑140 (MQ=255)

                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 >  2:3719764/1‑140 (MQ=255)

                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 <  1:5254709/140‑1 (MQ=255)

                                                                                        aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc                                               <  1:4678137/140‑1 (MQ=255)

                                                                                              cAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                         <  2:4770958/140‑1 (MQ=255)

                                                                                              cAGTTTGCCAGCGCCGCCTTCTCCGTCGCTACAATCGATCCGATT‑C‑C‑CGCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                         <  2:1651500/140‑1 (MQ=255)

                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTGTGCCGTCGCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  1:1651500/1‑140 (MQ=255)

                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  1:443194/1‑140 (MQ=255)

                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCCCTTCAT‑GGGTGAACGGCTCGGCTTCTGCCGATCCCTGCACTTCAATGGTGCGCCCGTCTTCg                                        >  2:5570756/1‑140 (MQ=255)

                                                                                                gTTTGCCAGCGCCGCTTTCTGCTGCTCTAGAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAGCAAGATGAGTACCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       <  1:1767031/140‑1 (MQ=255)

                                                                                                 tttGCCAGCGCCGCCTTCTGCGCCGCTACAATGGACTCGTTTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:1578935/140‑1 (MQ=255)

                                                                                                    gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  2:345806/140‑1 (MQ=255)

                                                                                                        gcgcCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  1:3649199/140‑1 (MQ=255)

                                                                                                        gcgcCGCCTTCTGCGGCGCTACAATCGATCCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                               <  1:1884472/140‑1 (MQ=255)

                                                                                                          gccgccTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTAc                             <  1:1356128/140‑1 (MQ=255)

                                                                                                          gccgccTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTAc                             <  1:3996458/140‑1 (MQ=255)

                                                                                                              ccTTCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                         <  1:2865290/140‑1 (MQ=255)

                                                                                                                ttCTGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCAt                       <  1:2925197/140‑1 (MQ=255)

                                                                                                                   tGCGTCGCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                    >  2:4810191/1‑140 (MQ=255)

                                                                                                                        cGCTACAATGGATTCTATT‑C‑C‑CTCTTGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtct               <  1:2045158/140‑1 (MQ=255)

                                                                                                                         gCTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCATCGTTCATCACTACGTTCATGTCGGtctc              >  1:1967197/1‑140 (MQ=255)

                                                                                                                          cTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct             <  2:36633/140‑1 (MQ=255)

                                                                                                                          cTACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCACCCGTCTACGGGCAGCACTACGTTCATGTCGGTctct             >  1:2730366/1‑140 (MQ=255)

                                                                                                                           tACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTg            >  2:3908856/1‑140 (MQ=255)

                                                                                                                           tACAATGGATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTg            <  2:3529654/140‑1 (MQ=255)

                                                                                                                                 ggATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag      <  2:682906/140‑1 (MQ=255)

                                                                                                                                 ggATTCGATTCC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag      <  2:4077970/140‑1 (MQ=255)

                                                                                                                                     tcgattcC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  1:149909/1‑140 (MQ=255)

                                                                                                                                     tcgattcC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  2:4508421/1‑140 (MQ=255)

                                                                                                                                     tcgattcC‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  1:75436/1‑140 (MQ=255)

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑C‑C‑CTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAT‑GGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815741‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A