Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F60 I1 R1
|
20 |
86.3 |
2971396 |
99.1% |
2944653 |
139.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,815,883 |
+C |
coding (667/687 nt) |
rph ← |
truncated RNase PH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,815,879 | 1 | . | C | 88.2%
| 168.1
/ 14.0
| 51 | coding (671/687 nt) | rph | truncated RNase PH |
| Reads supporting (aligned to +/- strand): ref base . (3/3); new base C (19/26); total (22/29) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
TAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGA > NC_000913/3815744‑3816009
|
tAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑ccc > 1:11366/1‑140 (MQ=255)
aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑cccct < 2:995970/140‑3 (MQ=255)
aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCATACTTTTCGACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑cccct < 2:599859/140‑3 (MQ=255)
aaCTGGCGCTGATATGGTTTCAGGCCTTCGCTCCTTATCTTACTTTTCAACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑c‑cccct < 2:610529/140‑3 (MQ=255)
cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGcc > 1:1476562/1‑140 (MQ=255)
gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCa < 1:1258413/140‑1 (MQ=255)
tatGGTTTCATGCCTTCTCTCCCCATCTTACGTTTTTACGGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGcc < 2:618618/140‑1 (MQ=255)
atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCca < 2:940120/140‑1 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATg > 2:1329788/1‑140 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATg > 2:880589/1‑140 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATg > 1:1156208/1‑140 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CTCCC‑TC‑GGGCAAGAGCCAACAAGATg > 1:506672/1‑140 (MQ=255)
aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAGGGATTCGATT‑CTCCC‑TC‑GGGCCAGAGCCAACAAGATg > 1:545022/1‑140 (MQ=255)
tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGa < 2:1476562/140‑1 (MQ=255)
tGCCTTCGCTCCTCATCTTACTGTTCTACAGACAATAAACAGGCGGCTTATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGa < 2:588501/140‑1 (MQ=255)
ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAg > 1:1350279/1‑140 (MQ=255)
ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAg < 2:11366/140‑1 (MQ=255)
ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAg < 2:1155997/140‑1 (MQ=255)
ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAg < 2:26758/140‑1 (MQ=255)
ttCGCTCCTCATCTTAATTTTCGACAGACAGAAGATAGGCGACTCATCGGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAg < 2:710390/140‑1 (MQ=255)
tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGc < 2:1156208/140‑1 (MQ=255)
cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAg < 2:953184/135‑1 (MQ=255)
cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAg > 1:953184/1‑135 (MQ=255)
cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCCATT‑C‑CCCATC‑GAGCCAGAGCCAACAAGATGAGTAGct > 1:710390/1‑140 (MQ=255)
tcctcATCTTACTTTTCTACAGCCAAAAAAAGGGCAACTGATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCtt < 2:506672/140‑1 (MQ=255)
tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCtt < 1:776625/140‑1 (MQ=255)
tcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt > 2:95012/1‑140 (MQ=255)
aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa > 1:1385787/1‑140 (MQ=255)
aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa > 2:227764/1‑140 (MQ=255)
ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg > 2:317269/1‑140 (MQ=255)
ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc < 2:1115358/140‑1 (MQ=255)
cAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCCACAAGATGAGTAGCCCTTCATGGGTGAACGGCTCGcc > 1:640705/1‑140 (MQ=255)
cGACTCATCAGTCGCCTTAAAATTCAGTTTGCCAGCGCCTCCTTCTGCTTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGACGTCCCCTg < 2:468887/140‑1 (MQ=255)
cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg < 2:1350279/140‑1 (MQ=255)
cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg < 1:227764/140‑1 (MQ=255)
aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTGAAGGGTGAACGGTTCGCCTTCTGCCGTCCCCTGCa > 2:669016/1‑140 (MQ=255)
gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg > 1:1341646/1‑140 (MQ=255)
ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgc > 2:1304104/1‑140 (MQ=255)
ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCCTGGGTGAACGGCGCGCGTTCTGCCGACCCCAGCACTTCAATGATgcgc > 2:385345/1‑140 (MQ=255)
ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAATGGCCCGCCTTCTGCCGGCCCCCGCACTTCAATGATgcgc > 2:589390/1‑140 (MQ=255)
ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc > 2:1249906/1‑140 (MQ=255)
ttAAAAATCAGTTTGACAGCGCCGCCTTCCGCGTCGCCACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc < 1:385345/140‑1 (MQ=255)
tAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc < 1:95012/140‑1 (MQ=255)
aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑CTCCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc < 1:847534/140‑1 (MQ=255)
aTCAGTTTGCCAGCGCCGCCTTTTGCGTCGCCACAGTGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt < 2:651526/140‑1 (MQ=255)
aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt < 2:1424489/140‑1 (MQ=255)
aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt < 1:1329788/140‑1 (MQ=255)
tCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCtt < 1:317269/140‑1 (MQ=255)
tttGCCAGCGCCGCCTTGTGCGTCGCTACAAGGGATTCGTTTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt < 2:545022/140‑1 (MQ=255)
tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt > 2:1088160/1‑140 (MQ=255)
tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt < 2:1385787/140‑1 (MQ=255)
cgccTTCTGCGTCGCTACAATGGATTCGATTCC‑CCC‑TG‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt < 1:669016/140‑1 (MQ=255)
cTACAATGGATTCGATTCC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTctct > 1:1080575/1‑140 (MQ=255)
tGGATTCGATT‑C‑CCC‑CCTGGGCCAGAGCCAACAAGTTGAATAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa < 2:652193/140‑1 (MQ=255)
tGGATTCGATT‑C‑CCC‑CCGGGGCTAGAGCCAACATGATGAGTAGCTCTTTATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa < 2:640705/140‑1 (MQ=255)
tcgattcC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga > 2:817468/1‑137 (MQ=255)
tcgattcC‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga < 1:817468/137‑1 (MQ=255)
|
TAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATT‑C‑CCC‑TC‑GGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGA > NC_000913/3815744‑3816009
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
