Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F60 I1 R1	20	81.1	2806374	99.2%	2783923	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	95.2%	220.8 / 3.1	62	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (22/37); total (24/38)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TT‑CC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815743‑3816012

aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TT‑cc‑c                                                                                                                                      >  2:1055333/1‑140 (MQ=255)

    aCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TT‑cc‑c‑cctc                                                                                                                                 >  1:90078/1‑137 (MQ=255)

       ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGACAGCGCCGGCTTTTGCATCGCTACACTGGAGTCGA‑TTCCC‑C‑C‑TCGGGc                                                                                                                             >  2:456009/1‑140 (MQ=255)

        gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGc                                                                                                                             <  2:1208657/140‑1 (MQ=255)

        gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGc                                                                                                                             <  2:1229430/140‑1 (MQ=255)

         cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGc                                                                                                                             <  2:1258685/140‑1 (MQ=255)

         cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGcc                                                                                                                            >  1:1251779/1‑140 (MQ=255)

         cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGcc                                                                                                                            >  1:608350/1‑140 (MQ=255)

         cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCa                                                                                                                           >  2:344193/1‑140 (MQ=255)

           cTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCag                                                                                                                          <  2:1304752/140‑1 (MQ=255)

            tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCaga                                                                                                                         <  2:1020146/140‑1 (MQ=255)

              atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCAGGCCAGAGc                                                                                                                       >  1:1121545/1‑140 (MQ=255)

               tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGcc                                                                                                                      <  1:729582/140‑1 (MQ=255)

                  ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCcaac                                                                                                                   <  2:37724/140‑1 (MQ=255)

                  ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCcaac                                                                                                                   <  2:1081799/140‑1 (MQ=255)

                    tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCcaacaa                                                                                                                 >  1:1239123/1‑140 (MQ=255)

                    tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAg                                                                                                                <  1:344193/140‑1 (MQ=255)

                       cATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCag                                                                                                                          <  1:260365/128‑1 (MQ=255)

                       cATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCag                                                                                                                          >  2:260365/1‑128 (MQ=255)

                       cATGCCTTCGCTCCTCATCTTACTTATCTACAGAC‑AACAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGAt                                                                                                              <  1:410110/140‑1 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATTAGTTTGCCAGCGCCGCCTTCTGAGTCGCTACGATGTATTAGA‑TT‑CC‑C‑CGTCGGACCAGAGCCAACAAGATg                                                                                                             >  2:396751/1‑140 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  2:1136253/1‑140 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:976014/1‑140 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:1024910/1‑140 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:1240298/1‑140 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:129767/1‑140 (MQ=255)

                        aTGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:1334096/1‑140 (MQ=255)

                         tGCCTTCGCTCCTCATTGTACTCTTCTACAAGC‑AATAAACAGGCGACTTATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGa                                                                                                            <  2:403382/140‑1 (MQ=255)

                         tGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑GT‑CCGC‑C‑TCGGGCCCGAGCCAACAAGATGa                                                                                                            >  1:631169/1‑140 (MQ=255)

                          gCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAg                                                                                                           <  1:870271/140‑1 (MQ=255)

                           ccTTCGCTCCTCATCTTACTTTACTACAGAC‑TAAACAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGt                                                                                                          <  2:444927/140‑1 (MQ=255)

                             ttCGCTCCTCATCTTACTTTTCTACAGAC‑CAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:518637/140‑1 (MQ=255)

                             ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:881464/140‑1 (MQ=255)

                             ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:1239123/140‑1 (MQ=255)

                             ttCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCAGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:1121545/140‑1 (MQ=255)

                             ttCGCTCCTCATCTTACTTTTCTACAGAC‑‑AAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGc                                                                                                       >  2:12822/1‑140 (MQ=255)

                                gCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGctc                                                                                                     >  1:243622/1‑140 (MQ=255)

                                  tcctcATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                   <  2:45367/140‑1 (MQ=255)

                                            aCTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                                                                         >  2:1091170/1‑140 (MQ=255)

                                              ttttCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAATATCAGTAGCACTTCATGGGTGAACg                                                                                       >  1:655761/1‑140 (MQ=255)

                                                   tACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  >  1:1058890/1‑140 (MQ=255)

                                                     cAGAC‑AAAAAAAAGGCGCCTCAACAGTCGCCGTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                <  2:631169/140‑1 (MQ=255)

                                                       gAC‑AAAAAAAAGGCGACTCATCCGTCGCCTTAAAAATCACTTTGCCAGAGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                              <  2:653535/140‑1 (MQ=255)

                                                           aaaaaaaaTGCGGCTCATCAGTCGACTTAAGAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TG‑CC‑CGC‑TCGGGCCAGAGCCAACAAGAGGAGTAGCTCTTCATGGGGTAACTGCTCGACTTCTg                                                                           >  2:871660/1‑140 (MQ=255)

                                                           aaaaaaaaGGCGACTTATCAGTAGCATTACAAATTAGATTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                           <  1:396751/140‑1 (MQ=255)

                                                                  aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    <  2:243622/140‑1 (MQ=255)

                                                                     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                         >  1:282228/1‑132 (MQ=255)

                                                                     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                         <  2:282228/132‑1 (MQ=255)

                                                                     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:1136253/140‑1 (MQ=255)

                                                                     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:1209081/140‑1 (MQ=255)

                                                                     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1240298/140‑1 (MQ=255)

                                                                     cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1334096/140‑1 (MQ=255)

                                                                       aCTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCTTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:592357/140‑1 (MQ=255)

                                                                       aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  1:1288911/1‑140 (MQ=255)

                                                                                    cTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                  <  2:90078/140‑1 (MQ=255)

                                                                                     ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 >  2:1096755/1‑140 (MQ=255)

                                                                                              aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  1:718318/1‑140 (MQ=255)

                                                                                                tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:12822/140‑1 (MQ=255)

                                                                                                tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  2:1058890/140‑1 (MQ=255)

                                                                                                 ttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGAAAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                     <  1:871660/140‑1 (MQ=255)

                                                                                                   gCCAGCGCCGCCTTCGGCGTCGATATAATTGATTCGACTC‑CC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  1:456009/140‑1 (MQ=255)

                                                                                                     cAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 <  2:1251779/140‑1 (MQ=255)

                                                                                                           cgccTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                           <  1:1055333/140‑1 (MQ=255)

                                                                                                           cgccTTCTGCGTCGCTACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                           <  2:608350/140‑1 (MQ=255)

                                                                                                                          tACAATGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTg            <  2:1288911/140‑1 (MQ=255)

                                                                                                                               tGGATTCGA‑TTCCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa       <  2:1024910/140‑1 (MQ=255)

                                                                                                                                  attcga‑ttcCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCagag    >  1:30424/1‑140 (MQ=255)

                                                                                                                                   ttcga‑ttcCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt   >  2:909675/1‑140 (MQ=255)

                                                                                                                                    tcga‑ttcCC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCACCCGTCTTCGGTCATCTCTACGTTCATGTCAGTCTCTGCGGCAGAGTc  >  2:596433/1‑140 (MQ=255)

ATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGAC‑AAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGA‑TT‑CC‑C‑C‑TCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815743‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A