Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F60 I1 R1 17 287.4 9819666 99.1% 9731289 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8791.C95.8% 714.8 / 16.3 193coding (671/687 nt)rphtruncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (6/2);  new base C (71/113);  total (77/116)
Fisher's exact test for biased strand distribution p-value = 6.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑T‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCT  >  NC_000913/3815741‑3816013
                                                                                                                                              |                                                                                                                                       
cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTAGCTACAATGgattcg‑a‑t‑t‑c                                                                                                                                        <  1:1002737/140‑1 (MQ=255)
       cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑T‑c‑cccctcg                                                                                                                                >  1:1094774/1‑136 (MQ=255)
       cTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑T‑c‑cccctcg                                                                                                                                >  1:4575678/1‑136 (MQ=255)
          gcgcTGATATGGTTTCATGCCTGCGGTCCCCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGc                                                                                                                              <  1:1875208/140‑1 (MQ=255)
           cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGcc                                                                                                                             >  1:3181150/1‑140 (MQ=255)
           cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGcc                                                                                                                             >  2:4713965/1‑140 (MQ=255)
               gATATGGTTTCATGCCTTCGCTCCTCATCTTCCTTTTCTCCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCagag                                                                                                                         <  2:1700520/140‑1 (MQ=255)
                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTTCGTGGCTGCAATGGATTCG‑A‑TAC‑C‑CCCTCGGGCCAGAGc                                                                                                                        >  2:1617932/1‑140 (MQ=255)
                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGc                                                                                                                        >  2:3928593/1‑140 (MQ=255)
                atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAACCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACATTGGATTCG‑A‑T‑T‑CACCCTCGGGCCAGAGc                                                                                                                        >  2:3805586/1‑140 (MQ=255)
                     gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCcaaca                                                                                                                   <  2:1143202/140‑1 (MQ=255)
                     gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCcaaca                                                                                                                   <  2:418603/140‑1 (MQ=255)
                       ttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg                                                                                                                 <  1:4178280/140‑1 (MQ=255)
                       ttCATGCCTTCGCTCCTAATCTAACTTTTCTACAGACAACAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAg                                                                                                                 <  2:1774142/140‑1 (MQ=255)
                        tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGa                                                                                                                >  2:1034055/1‑140 (MQ=255)
                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGTCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGc                                                                                                                              >  1:1039475/1‑124 (MQ=255)
                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACTATGAAATCG‑A‑T‑TCC‑CCCTCGGATCAGAGCCAACAAGATg                                                                                                              >  2:2196677/1‑140 (MQ=255)
                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATg                                                                                                              >  1:3832229/1‑140 (MQ=255)
                          aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCACCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGcc                                                                                                                       >  1:784111/1‑131 (MQ=255)
                            gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                            <  2:2922834/140‑1 (MQ=255)
                               ttCGCTCCTCATCTTGCTTTTCTACTGACAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:2016120/140‑1 (MQ=255)
                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  2:3376851/140‑1 (MQ=255)
                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  2:3059260/140‑1 (MQ=255)
                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:685298/140‑1 (MQ=255)
                               ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                         <  1:623578/140‑1 (MQ=255)
                                tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGc                                                                                                        >  2:4455966/1‑140 (MQ=255)
                                 cGCTCCTCATCTTACCTTTCTATAGCCAAGAAAAAGGGGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGct                                                                                                       <  1:1732849/140‑1 (MQ=255)
                                  gCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                                                                                      >  1:821143/1‑140 (MQ=255)
                                        cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                >  2:3692801/1‑140 (MQ=255)
                                        cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                                >  2:2878537/1‑140 (MQ=255)
                                         aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                                                                                      <  2:3521760/133‑1 (MQ=255)
                                         aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGctc                                                                                                      >  1:3521760/1‑133 (MQ=255)
                                           cTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                                                                             >  1:560031/1‑140 (MQ=255)
                                            ttACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                                                                            >  2:1099962/1‑140 (MQ=255)
                                              aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGTCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑AGT‑C‑C‑CCCTCGGGCTAGATCCAACAAGGTGAGAAGCTCTTCATGGGTGaa                                                                                          >  2:1536127/1‑140 (MQ=255)
                                               cTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                                                                         <  1:2878537/140‑1 (MQ=255)
                                               cTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                                                                         <  2:253812/140‑1 (MQ=255)
                                                ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                    >  2:1225940/1‑128 (MQ=255)
                                                  ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                      <  2:4800815/140‑1 (MQ=255)
                                                  ttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                      <  1:4190742/140‑1 (MQ=255)
                                                    cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTGGCTCTTGATGGGTGAACGGCTc                                                                                    >  1:1638437/1‑140 (MQ=255)
                                                    cTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                                                                                    <  1:3692801/140‑1 (MQ=255)
                                                     tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                   >  1:2477811/1‑140 (MQ=255)
                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTc                                                                              >  1:4865676/1‑140 (MQ=255)
                                                          aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAACTCTTCATGGGTGAACGGCTCGCCTTc                                                                              >  1:779685/1‑140 (MQ=255)
                                                            aaaaaaaaGGCGACTCATCAGTGGCCTTATAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                            <  1:2106281/140‑1 (MQ=255)
                                                            aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                            <  2:1094774/140‑1 (MQ=255)
                                                            aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                            >  1:2718563/1‑140 (MQ=255)
                                                            aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                                                                            <  1:4378710/140‑1 (MQ=255)
                                                              aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                          <  2:248235/140‑1 (MQ=255)
                                                              aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                          <  2:4092797/140‑1 (MQ=255)
                                                              aaaaaaGGCGACTCATCAGTCGCATTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                          <  1:4038544/140‑1 (MQ=255)
                                                                 aaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                       <  2:3181150/140‑1 (MQ=255)
                                                                   aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                     <  1:161307/140‑1 (MQ=255)
                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:4579678/140‑1 (MQ=255)
                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:789277/140‑1 (MQ=255)
                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:2796524/140‑1 (MQ=255)
                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:4713965/140‑1 (MQ=255)
                                                                      cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  2:3353716/140‑1 (MQ=255)
                                                                       gACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc                                                                 >  1:237337/1‑140 (MQ=255)
                                                                        aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                                >  1:4777393/1‑140 (MQ=255)
                                                                                gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  <  1:2608516/130‑1 (MQ=255)
                                                                                gTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                  >  2:2608516/1‑130 (MQ=255)
                                                                                   gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                     >  2:3098679/1‑140 (MQ=255)
                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                  >  1:1156730/1‑140 (MQ=255)
                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                  >  2:2657928/1‑140 (MQ=255)
                                                                                      ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                  >  2:599202/1‑140 (MQ=255)
                                                                                        aaaaaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGccc                                                >  1:531679/1‑140 (MQ=255)
                                                                                            aTCAGTTTGCCAGCGGCGCCTTGTGGGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                            <  1:1615556/140‑1 (MQ=255)
                                                                                            aTCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                            <  1:4224257/140‑1 (MQ=255)
                                                                                               aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                         >  2:3770644/1‑140 (MQ=255)
                                                                                                gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt                                            >  2:782747/1‑136 (MQ=255)
                                                                                                gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                        <  1:3770644/140‑1 (MQ=255)
                                                                                                gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCAGCCGTCCGCTGCACATCAATGATGCGCCCGTCTTCgg                                        >  1:1811413/1‑140 (MQ=255)
                                                                                                  ttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtc                                      <  2:1156730/140‑1 (MQ=255)
                                                                                                   tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCTTCGGt                                       <  1:3310090/138‑1 (MQ=255)
                                                                                                   tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATTATGCGCCCGTCTTCGGt                                       >  2:3310090/1‑138 (MQ=255)
                                                                                                   tGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                     <  1:3098679/140‑1 (MQ=255)
                                                                                                   tGCCAGCGCCGCCTTCTGCGTCCCTCCAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                     <  1:2364103/140‑1 (MQ=255)
                                                                                                   tGCCAGCGCCGCCTTCTCCGTCGCTACAATGGATTGG‑A‑TAC‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                     <  2:1811413/140‑1 (MQ=255)
                                                                                                    gCCAGCGCCGCCTTCAGCGTCGCTACAATCGATTCG‑ACT‑C‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                    <  1:1389142/140‑1 (MQ=255)
                                                                                                      cAGCGCCGCCTTCTGCGTCGCTGCACTGGTTTCG‑A‑T‑TAC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                  <  1:2196677/140‑1 (MQ=255)
                                                                                                          gccgccTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                 >  1:3894573/1‑137 (MQ=255)
                                                                                                          gccgccTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGCCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                 <  2:3894573/137‑1 (MQ=255)
                                                                                                            cgccTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGt                            <  2:860857/140‑1 (MQ=255)
                                                                                                              ccTTCTGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                          <  1:1232186/140‑1 (MQ=255)
                                                                                                                   tGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                     >  1:2881322/1‑140 (MQ=255)
                                                                                                                   tGCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                     >  1:2440407/1‑140 (MQ=255)
                                                                                                                    gCGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                    <  1:2616148/140‑1 (MQ=255)
                                                                                                                     cGTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCgg                   >  1:1122246/1‑140 (MQ=255)
                                                                                                                      gTCGCTACAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGt                  <  2:2422186/140‑1 (MQ=255)
                                                                                                                        cGCTACAACGGATTCGCG‑T‑G‑C‑CCCTCGGGCTTGCGTCAATAAGATGAGCAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCGCTGCATTTCAATGATGCGCTCGTCTTCGGTCATCACTACGTTCATGTCGGtct                <  2:2365797/140‑1 (MQ=255)
                                                                                                                            aCAATGGATTCG‑A‑T‑TCC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGTCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGc            <  2:821143/140‑1 (MQ=255)
                                                                                                                                 ggATTCG‑A‑T‑TCC‑CCCTCGGGCCTGAGCCAACTAGATGAGCAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCag       <  2:1857203/140‑1 (MQ=255)
                                                                                                                                  gattcg‑a‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga      <  2:190402/140‑1 (MQ=255)
                                                                                                                                  gattcg‑a‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga      >  2:1074546/1‑140 (MQ=255)
                                                                                                                                    ttcg‑a‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCTGTCATCAATACTTTAATGTCGGTCTCTGCGGCAGAGt    >  1:1857203/1‑140 (MQ=255)
                                                                                                                                    ttcg‑a‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGt    >  1:3744825/1‑140 (MQ=255)
                                                                                                                                     tcg‑a‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc   >  1:1079799/1‑140 (MQ=255)
                                                                                                                                      cg‑a‑t‑tcC‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt  <  2:788968/140‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                       
CAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑T‑T‑C‑CCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCT  >  NC_000913/3815741‑3816013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A